MOLECULAR DIAGNOSIS OF NEMATODE WORMS PARABRONEMA SKRJABINI IN CAMELS (Camelus dromedaries) IN IRAQ
DOI:
https://doi.org/10.36103/ijas.v53i3.1567Keywords:
Parabronema skrjabini, Nematoda, phylogenic tree, camels, IraqAbstract
The current study was designed for analysis of the pattern of the nucleotide sequence of the tissue DNA isolates based on the ITS2,28SrDNA gene by the traditional polymerase chain reaction. All amplicons were well suited for the prepared primer with size 873 bp . and identical ratio ranged 87.2- 98% of the same species, as included a high similarity of the isolates taken camels in Iran and Iraq related to Parabronema skrjabini . Phylogenic tree inferred the degree of relatedness between 28SrDNA sequences deposited in the international nucleotide bank sequence database (NCBI). The sequence of Parabronema skrjabini was recorded in the Genbank under accession number MT742154.1.
References
Bekele, T., 2002. Epidemiological studies on gastrointestinal helminths of dromedary (Camelus dromedarius) in semi-arid lands of eastern Ethiopia. Veterinary parasitology. 105(2): 139-152
Borji, H.;G. Razmi ; A.R. Movassaghi ;A.G. Naghibi and M. Maleki ,2010. A study on gastrointestinal helminths of camels in Mashhad abattoir, Iran. Iranian Journal of Veterinary Research. 11: 174-179.
Bowman, D.D. 2009.Georgis’ Parasitology for Veterinarians.9th Ed. Westline industrial drive St. Louis, Missouri, pp.142
Chhabra, M.B. and S.K Gupta, 2006. Parasitic diseases of camels-an update, 2. Helminthoses. Journal of Camel Practice and Research. 13(2): 81–87
Faraj,A.A; B. F. Hade and A. M. Al- Amery,2019. Conventional and molecular study Babesia spp. of natural infection in dragging horses at some areas of Baghdad city, Iraq. Iraqi Journal of Agricultural Sciences.50(3):909- 915
Faraj,A.A and A. M. Al- Amery,2020. Microscopic and molecular diagnosis of Ascaridia spp. in domestic pigeons (Columba livia domestica) in Baghdad city ,Iraq. Iraqi Journal of Agricultural Sciences .51(4):1220-1225
Hasheminasab, S.S., 2015. Molecular characterization of the first internal transcribed spacer of rDNA of Parabronema skrjabini for the first time in sheep. Annals of Parasitology. 61(4): 241-246
Hasheminasab, S.S.;F. Jalousian; and B. Meshgi, 2016. Molecular and morphological characterization of Parabronema skrjabini of sheep and goats at three different geographical zones in Iran. Annals of Parasitology. 62(1), 55–61
Kassai ,T. 1999. Veterinary Helminthology, Butterworth-Heineman Publishing, Oxford, UK.: pp 222
Magzoub, M.; O. Omer ;E. Haroun and O. Mahmoud , 2000. Effect of season on gastrointestinal nematode infection in Saudi Arabian camels (Camelus dromedarius). Journal of Camel Practice and Research, 7: 107-108
Otranto D.;E. Tarsitano ;D. Traversa ;A. Giangaspero ;F. De Luca and V. Puccini , 2001. Differentiation among three species of bovine Thelazia (Nematoda: Thelaziidae) by polymerase chain reaction-restriction fragment length polymorphism of the first internal transcribed spacer ITS-1 (rDNA). International Journal for Parasitology. 31: 1693-1698
Swai, E. S.;W. Moshy; D. Mshanga; L. Lutatina and S. Bwanga, 2011. Intestinal parasitic infections of camels in the agro and pastoral areas of northern Tanzania. Vet Res. 4(2); 34-8
Sweeney, T.;J.P. Hanrahan; M.T. Ryan and B. Good, B. 2016. Immunogenomics of gastrointestinal nematode infection in ruminants–breeding for resistance to produce food sustainably and safely. Parasite immunology. 38(9): 569-586
Tafti, M.; A. Sazmand; S. Hekmatimoghaddam and I Moobedi, 2013. Gastrointestinal helminths of camels (Camelus dromedarius) in the center of Iran. Trop Biomed. 30: 56-61
Traversa D.,A. Giangaspero ;R. Iorio ;D. Otranto ;B. Paoletti and R. Gasser , 2004. Semi-nested PCR for the specific detection of Habronema microstoma or Habronema muscae DNA in horse faeces. Parasitology. 129: 733-739
Zajac, A.M. and T.A. Gipson, 2000. Multiple anthelmintic resistance in a goat herd. Veterinary Parasitology. 87(1-2): 163-172.
Zhang ,X.D.;X.Y. Yang ; L.R. Yang ; L.C. Li ;H.T. Zuo ;R.H. Na ;Z.G. Zhao and J.J Wang, 2009. Cloning and sequence analysis of the rDNA-ITS of Parabronema skrjabini . Chinese Journal of Animal and Veterinary Sciences 5: 26-30.
Downloads
Published
Issue
Section
License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
How to Cite
2.jpg)
https://orcid.org/0000-0002-5774-5906 2.jpg)
