SOME BIOCHEMICAL ATTRIBUTES IN SEMINAL PLASMA OF IRAQI BUFFALO BULLS AND THEIR RELATION TO THE SEMEN QUALITY

Authors

  • K. S. Musa
  • T. A. Abdulkareem

DOI:

https://doi.org/10.36103/nrfkex70

Keywords:

cholesterol, triglycerides, semen, fertility prediction, Buffalo.

Abstract

This study was undertaken to figure out the relationship between some biochemical characteristics in seminal plasma with the semen quality of Iraqi buffalo bulls (Bubalus bubalis). Semen was diluted with the Tris extender and divided based on the sperm cell's individual motility into two groups. The first group included good-semen quality (GS; n=3), whereas, the second group included poor-semen quality (PS; n=5). The differences between GS and BS groups in all seminal biochemical attributes lacked significance.  A negative and significant (P≤0.05) correlation coefficient was noticed between cholesterol concentration and both principal and terminal sperms abnormality (r= -0.52), and total abnormalities (r= -0.53). A positive and significant (P≤0.05) correlation coefficient was shown between cholesterol concentration and normal sperm percentage (r= 0.53). Moreover, there was a negative and significant correlation (P≤0.05) between the triglyceride concentration and each of the percentages of individual sperm motility (r = -0.50) and of live sperm (r = -0.51).  It can be concluded that some biochemical characteristics of seminal plasma can be used to predict the semen quality and fertility of Iraqi buffalo bulls.

References

Abdulkareem, T.A. 2013a. Some hematological and blood biochemical profiles of Iraqi riverine buffaloes (Bubalus bubalis) during different gestation period. J. Buffalo Sci. (India), 2, 78-84. https://doi.org/10.6000/1927-520X.2013.02.02.4

Abdulkareem, T. A. 2013. Some hematological and blood biochemical attributes of Iraqi riverine buffaloes (Bubalus bubalis) around calving and post-partum period. Al-Anbar J. Vet. Sci., 6 (1),143-150

Abdulkareem, T. A. and O. H. Al-Zaidi. 2018. Effect of adding aqueous extract of Melissa officinalis leaves and some other antioxidants to milk–based extender on post-cooling and post-cryopreservative sperm's individual motility and live sperm percentage of Holstein bulls. Al-Anbar J. Vet. Sci., 11 (1), 37-53

Abdulkareem, T. A., K. H. Sultan and M. S. Noon. 2017. The synergistic effect of some antioxidants added to Tris extender on semen freezability of Holstein bulls following different cryopreservation periods. Al-Anbar J. Vet. Sci., 10 (1), 1-9

Abdulkareem, T. A., S. M. Eidan, M. A. Ishak, S. A. M. Al-Sharifi, M. A. Alnimr, C. W. Passavant, J. R. Branen and R. G. Sasser. 2012. Pregnancy- specific protein B (PSPB), progesterone and some biochemical attributes concentrations in the fetal fluids and serum and its relationship with fetal and placental characteristics of Iraqi riverine buffalo (Bubalus bubalis) Anim. Reprod. Sci., 130, 33- 41. https://doi.org/10.1016/j.anireprosci.2012.01.002

Abdulkareem, T. A., S. M. Eidan, F. K. Alsaidy and N. K. Al-Hassani. 2023. Effect of pre-and post-mating vitamin AD3E treatment on reproductive performance of Awassi ewes. Iraqi Journal of Agricultural Sciences, 54(2), 431-437. https://doi.org/10.36103/ijas.v54i2.1717

Al-Helal, A. M. A. 2023. Detection of Some Biomarkers in the Seminal Plasma and Effect of Adding Resveratrol to the Different Types of Extenders on Semen Quality of the Iraqi Buffalo Bulls Reared in the Artificial Insemination Center After Cooling and Post-cryopreservation for Different Periods. Ph.D. Thesis, College of Agricultural Engineering Sciences, University of Baghdad

Alhelal, A. M. and T. A. Abdulkareem. 2023. Effect of adding resveratrol to soybean-lecithin extender on some semen attributes of buffalo bulls. Iraqi Journal of Agricultural Sciences, 54 (4): 1074-1083. https://doi.org/10.36103/ijas.v54i4.1797

Alhelal, A. M. and T. A. Abdulkareem. 2024. Ameliorating post-thawed semen of buffalo bulls using a milk-based extender supplemented with resveratrol. Iraqi Journal of Agricultural Sciences, 55 (Special Issue), 186-194

Al-Saedi, A. J. A. and T. A. Abdulkareem. 2022. Comparison of semen quality for three lines of Holstein bulls. 1. Some immediate and microscopic characteristics. Iraqi Journal of Agricultural Sciences, 53 (4), 752-759. https://doi.org/10.36103/ijas.v53i4.1585

Almadaly, E. A., A. B. S. Abdel-Salam, F. M. Sahwan, K. A. Kahilo, T. K. Abouzed, and W. B. El-Domany. 2022. Fertility-associated biochemical components in seminal plasma and serum of buffalo (Bubalus bubalis) bulls. Front Vet Sci., 9,‏ 1043379. https://doi.org/10.3389/fvets.2022.1043379

AL-Nuaimi, A. J., T. A. Abdulkareem, F. F. Ibrahim, Z. A. Humade and F. A. Hussein. 2020. effect of adding Olea europaea and Rosmarinus officinalis aqueous extracts and calcium chloride to Tris extender on post-cryopreservative sperm’s cell individual motility and live sperm percentage for low semen quality of Holstein bulls. ‏ Biochem. Cell. Arch., https://doi.org/10.35124/bca.2020.20.1.519

Al-Nuaimi, A. J. and Abdulkareem, T. A. 2020. Effect of adding Olea europaea and Rosmarinus officinalis aqueous extracts and calcium chloride to Tris extender on post-cryopreserved sperms abnormality percentage for low semen quality of Holstein bulls. Biochem. Cell. Arch., 20 (1): 493-498. https://doi.org/10.35124/bca.2020.20.1.493

Bucci, D., E. Giaretta, M. Spinaci, G. Rizzato, G. Isani, B. Mislei, G. Mari, C. Tamanini and G. Galeati. 2016. Characterization of alkaline phosphatase activity in seminal plasma and in fresh and frozen– thawed stallion spermatoz-oa. Theriogenology, 85(2), 288-295.‏ https://doi.org/10.1016/j. theriogenology.2015.09.007

Cardozo, A., M. Fernandez, F. Forcada, A. Abecia, T. Muino- Blanco and A. Cebrian-Perez. 2006. Monthly variation in ovine seminal plasma proteins analyzed by 2D SDS-PAGE. Theriogenology, 66 (4), 841-850. https://doi.org/10.1016/j.theriogenology.2006.01.058

Chavda, B. P., K. B. Vala, V. K. Singh, G. B. Solanki and S. G. Prajapati. 2022. Caffeine supplementation in semen extender protects buffalo spermatozoa from cryodamage. Int. J. Vet. Sci. and Biotech., 18 (4), 1-5. https://doi.org/10.48165/ijvsbt.18.4.01

Divyashree, B. C., and S. C. Roy. 2018. Species-specific and differential expression of BSP-5 and other BSP variants in normozoospermic and asthenozoospermic buffalo (Bubalus bubalis) and cattle (Bos taurus) seminal plasma. Theriogenology, 106, 279-286. https://doi.org/10.1016/j.theriogenology.2017.10.014

Dixit, S., V. Pandey, D. K. Swain, R. Nigam, A. Sharma, D. Sharma and P. Singh. 2016. Seminal plasma and sperm membrane proteins of buffalo and cattle bulls. Buffalo Bulletin,35 (3), 437-443.‏ https://kuojs.lib.ku.ac.th/index.php/BufBu/article/view/1122

Eidan, S. M. 2016. Effect on post-cryopreserved semen characteristics of Holstein bulls of adding combinations of vitamin C and either catalase or reduced glutathione to Tris extender. Anim. Reprod. Sci., 167, 1-7. https://doi.org/10.1016/j.anireprosci.2016.01.014

Eidan, S.M., A.J. Al-Nuaimi, O.A. Abd Sultan, F.F. Ibrahim, T.A. Abdulkareem, and W.E. Lateef 2020. Effect of adding α-lipoic acid on some post-cryopreserved semen characteristics of Holstein bulls. Plant Archives, 20(2), 11-16.

Eidan, S. M., Al-Nuaimi, A. J., Sultan, O. A. A., Ibrahim, F. Fpost-COVID-19., Abdulkareem, T. A. and Lateef, W. Y. 2020. Effect of adding α-lipoic acid on some-cryopreserved semen characteristics of Holstein bulls. Plant Archives, 20: 11-16

Eidan, S.M., R. I. Khalil and A.F. Naser. 2024. Some of fatty acid and semen characteristics of Holstein bulls as influenced by different sperm freezability. Iraqi J. Agric. Sci.,55(2), [In Press].

Eidan, S. M. and S. A. Khudhir. 2023. Association between ATP1A1 gene polymorphisms with semen characteristics in Holstein bulls Iraqi Journal of Agricultural Sciences, 54 (2), 330-337. https://doi.org/10.36103/ijas.v54i2.1706

El-Sayed, R. A., A. El-Badry, M. Abu-Ahmed and A. M. Ghallab. 2014. Studying the lipids, antioxidants and enzymatic profiles in seminal plasma and blood serum of buffalo bulls in relation to fresh semen quality. J. Egypt. Vet. Med. Assoc., 14 (1), 147-161

Ezz, M. A., M. Hussein, A. Eldesouky, M. Badr, A. Balboula and S. M. Zaabel. 2017. The effect of cholesterol loaded cyclodextrins on post-thawing quality of buffalo semen in relation to sperm DNA damage and ultrastructure. Biol. Reprod.,17 (1), 42-50.‏ https://doi.org/10.1016/j.repbio.2016.12.001

Hassan, M. S., and S. M. Eidan. 2021. Effect of swim-up and glass wool techniques, with adding antioxidants to Tris extender on improving post-cryopreserved some semen attributes of low semen quality for Holstein bulls. Iraqi Journal of Agricultural Sciences, 52 (3), 552-563. https://doi.org/10.36103/ijas.v52i3.1342

Hassan, M. S., S. M. Eidan, F. F. Ibrahim and K. J. Yahya. 2021. Effect of swim-up and glass wool techniques, with adding antioxidants to Tris extender on improving post-cryopreserved total sperm characteristics in straw and freezability percentage for low semen quality of Holstein bulls. Iraqi Journal of Agricultural Sciences, 52 (4), 885-895. https://doi.org/10.36103/ijas.v52i4.1396

Iskandar, H., G. Andersson, H. Sonjaya, R. L. Arifiantini, S. Said, H. Hasbi, T. Maulana and A. Baharun. 2023. Protein identification of seminal plasma in Bali bull (Bos javanicus). Animals, 13(3), 514. https://doi.org/10.3390/ani13030514

Kadoom, A. K., F. E. El-Keraby, A. F. Ashour, and E. M. El-Siefy. 2016. Seminal plasma proteins as potential markers of semen characteristics in buffalo and bovine bulls. J. Anim. Poult. Prod.,7(8), 287-291. https://doi.org/10.21608/JAPPMU.2016.48715

Khoshvaght, A., A. Towhidi, A. Zare-shahneh, M. Noruozi, M. Zhandi, N. D. Davachi and R. Karimi. 2016. Dietary n-3 PUFAs improve fresh and post-thaw semen quality in Holstein bulls via alteration of sperm fatty acid composition. Theriogenology, 85 (2), 807-812. https://doi.org/10.1016/j.theriogenology. 2015.10.023

Longobardi, V., G. Albero, C. De Canditiis, A. Salzano, A. Natale, A. Balestrieri. and B. Gasparrini. 2017. Cholesterol-loaded cyclodextrins prevent cryocapacitation damage in buffalo (Bubalus bubalis) cryopreserved sperm. Theriogenology,89:359-364. https://doi.org/10.1016/j. theriogenology. 2016.09.048

Lu, J. C., J. Jing, Q. Yao, K. Fan, G. H. Wang, R. X. Feng and B. Yao. 2016. Relationship between lipids levels of serum and seminal plasma and semen parameters in 631 Chinese sub-fertile men. PLoS One, 11(1), e0146304.‏ https://doi.org/10.1371/journal.pone.0146304

Luo, I. X., X. Ren, S. Huang, Y. Li, Q. Xue, D. Shi and X. Li. 2023. Seminal plasma lipid profiles of differential cryotolerance of semen in Mediterranean buffalo bulls. Reprod. Domest. Anim., 58 (4), 481-485. https://doi.org/10.1111/rda.14308

K. S. Musa and T. A. Abdulkareem. 2023. Protein profiles in seminal plasma of Iraqi buffalo bulls (Bubalus bubalis) associated with fresh and cryopreserved semen quality. IOP Conf. Ser.: Earth Environ. Sci. 1262: 072095

Sharma, L., V. Pandey, R. Nigam, A. Saxena and D. K. Swain. 2015. Association of semen attributes and seminal plasma proteins of buffalo bulls. J. Anim. Res., 5(1), 119-123. https://doi.org/10.5958/2277-940X.2015.00020.0

Srivastava, N. and M. Pande 2017. Semen Analysis: An Overview. Protocols in Semen Biology (Comparing Assays): 1-6. https://doi.org/10.1007/978-981-10-5200-2_1

Srivastava, N., S. K. Srivastava, S. K. Ghosh, A. Kumar, P. Perumal and A. Jerome. 2013. Acrosome membrane integrity and cryocapacitation are related to cholesterol content of bull spermatozoa. Asian Pac. J. Anim. Reprod., 2(2), 126-131. https://doi.org/10.1016/S2305-0500(13)60132-3.

Sultan, O. A. A. and S. M. Eidan. 2020. Association of CD9 gene with semen quality of Holstein bulls: 1. Fresh semen. Biochem. Cell. Arch., 20 (1), 2721-2725. https://doi.org/10.35124/bca.2020.20.1.2721

Tapaloaga, D., M. K. H. AL-dulaimi, and P. R. Tapaloaga. 2018. Quality and quantity Parameters In buffalo semen production. scientific papers: Series d, animal Science- The International Session Of Scientific Communications Of The Faculty Of Animal Science, ISSN-L., 61(1):‏ 2285-5750.

Turaja, K. I. B., R. S. Vega, T. A. Saludes, A. G. Tandang, J. A. N. Bautista, A. J. Salces and C. M. Rebancos. 2019. Influence and total antioxidant capacity of non-enzymatic antioxidants on the quality and integrity of extended and cryopreserved semen of Murrah buffalo (Bubalus bubalis). Philippine J. Sci., 148 (4), 619-626.

Vale, W. G., G. N. Purohit, M. Y. Miyasaki and M. Gaur. 2014. Semen characteristics and artificial insemination in the buffalo. In: Bubaline Theriogenology. G. N. Purohit (Ed.), Chapter 29, International Veterinary Information Service, Ithaca, New York, USA. www.ivis.org pp, 524-559

Velho, A. L. C., E. Menezes, T. Dinh, A. Kaya, E. Topper, A. A. Moura and E. Memili. 2018. Metabolomic markers of fertility in bull seminal plasma. PLOS ONE, 13 (4), e0195279. https://doi.org/10.1371/journal. pone.0195279

Willforss, J., J. M. Morrell, S. Resjö, T. Hallap, P. Padrik, V. Siino, and P. Humblot. 2021. Stable bull fertility protein markers in seminal plasma. J. Proteom., 236, 104135.‏ https://doi.org/10.1016/j.jprot. 2021.104135

Zhu, Z., W. Zhang, R. Li and W. Zeng. 2022. Reducing the glucose level in pre-treatment solution improves post-thaw boar sperm quality. Front. Vet. Sci., 9, 856536. https://doi.org/10.3389/fvets.2022.856536

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Published

2024-02-25

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How to Cite

K. S. Musa, & T. A. Abdulkareem. (2024). SOME BIOCHEMICAL ATTRIBUTES IN SEMINAL PLASMA OF IRAQI BUFFALO BULLS AND THEIR RELATION TO THE SEMEN QUALITY . IRAQI JOURNAL OF AGRICULTURAL SCIENCES, 55(1), 402-412. https://doi.org/10.36103/nrfkex70

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