EVALUATION OF THE BIOLOGICAL EFFECT SYNTHESIZED ZINC OXIDE NANOPARTICLES ON PSEUDOMONAS AERUGINOSA

Authors

  • M. A. Alaa Alden
  • L. A. Yaaqoob

DOI:

https://doi.org/10.36103/ijas.v53i1.1502

Keywords:

ZnO nanoparticles, antimicrobial activity, prodigiosin.

Abstract

This study was aimed to demonstrate the biosynthesis procedure of Zinc oxide nanoparticles (ZnO NPs) using Prodigiosin pigment which in turn produced from particular environmental bacteria isolates Serratiamarcescensas a stabilizing and reducing agent. Additionally, the synthesis conditions were precisely taken into consideration as a pH of 7 and a temperature of 50°C alongside a concentration of prodigiosin of 12 mg/ml with 5mg precursor of zinc acetate in deionized distilled water (DDW) 50ml. Biosynthesized ZnO nanoparticles have presented many applications such as catalysis, biosensing, anticancer, and biomedical, etc. The optimum condition for ZnO biosynthesis was characterized through several techniques such as UV-Vis, AFM, XRD, FT-IR, and FE-SEM. In particular, a cut-off phenomenon of the biological synthesized ZnO was found at around 280 nmusing UV-Vis, while spherical shape particles were noticed using FE-SEM techniques. Also, the AFM analysis revealed that ZnO NPs an average diameter size of 45.02 nm.And the effect of ZnO NPs on bacteriaPseudomonas aeruginosaon an inhibition zone 29mm.

References

A.C. Gales, P.L. Torres, D.S. Vilarinho, R.S. Melo, C.F. Silva, R.F.Cereda and Carbapenem.2004.Resistant Pseudomonas aeruginosa outbreak in an intensive care unit of a teaching hospital, Brazilian Journal of Infectious Diseases, 8: 267-271

A.A. Ramos, A. Azqueta, C. Pereira-Wilson and A.R. Collins.2010. Polyphenolic compounds from Salvia species protect cellular DNA from oxidation and stimulate DNA repair in cultured human cells, Journal of agricultural and food chemistry, 58 :7465-7471

B. Forbes, D. Sahm, and A. Weissfeld.2002. Laboratory methods for detection of antibacterial resistance, Bailey & Scott1s Diagnostic Microbiology, 230-231

D. Ferguson. 2008. A study of clinical strains of Pseudomonas aeruginosa and the investigation of antibiotic resistance mechanisms in the multidrug resistant strain PA13, in, Dublin City University

E.A. Menezes, L.A. Silveira, F.A. Cunha, M.d.S. Cavalcante, A.B. Teixeira, I.R.N. Oliveira and M.N.C. Salviano.2003. Perfil de resistência aos antimicrobianos de Pseudomons isoladas no Hospital Geral de Fortaleza, Rev. bras. anal. clin, 177-180

E.Bergogne-Berezin.2004. Pseudomonas and miscellaneous gram-negative bacilli, Infectious diseases, 2 : 2203-2226

E. Rokhsat, O. Akhavan.2016. Improving the photocatalytic activity of graphene oxide/ZnO nanorod films by UV irradiation, Applied Surface Science, 371 : 590-595

E.M. Tretter, J. M. Berger. 2012. Mechanisms for defining supercoiling set point of DNA gyrase orthologs II. The shape of the gyra subunit C-terminal domain (CTD) is not a sole determinant for controlling supercoiling efficiency, Journal of Biological Chemistry, 287 : 18645-18654

E.Y. Salih, M.F.M. Sabri, S.T. Tan, K. Sulaiman, M.Z. Hussein, S.M. Said and C.C. Yap.2019. Preparation and characterization of ZnO/ZnAl 2 O 4-mixed metal oxides for dye-sensitized photodetector using Zn/Al-layered double hydroxide as precursor, Journal of Nanoparticle Research, 21 :55

E.Y. Salih, M.F.M. Sabri, M.H. Eisa, K. Sulaiman, A. Ramizy, M.Z. Hussein and S.M. Said, Mesoporous ZnO/ZnAl2O4 mixed metal oxide-based Zn/Al layered double hydroxide as an effective anode material for visible light photodetector, Materials Science in Semiconductor Processing, 121 105370

E.Y. Salih, M.F.M. Sabri, K. Sulaiman, M.Z. Hussein, S.M. Said, R. Usop, M.F.M. Salleh and M.B.A. Bashir.2018. Thermal, structural, textural and optical properties of ZnO/ZnAl2O4 mixed metal oxide-based Zn/Al layered double hydroxide, Materials Research Express, 5 : 116202

E.Y. Salih, M.F.M. Sabri, M.Z. Hussein, K. Sulaiman, S.M. Said, B. Saifullah and M.B.A. Bashir. 2018. Structural, optical and electrical properties of ZnO/ZnAl 2 O 4 nanocomposites prepared via thermal reduction approach, Journal of Materials Science, 53 : 581-590

F. Malega, I. Indrayana, E. Suharyadi. 2018. Synthesis and characterization of the microstructure and functional group bond of fe3o4 nanoparticles from natural iron sand in Tobelo North Halmahera, Jurnal Ilmiah Pendidikan Fisika Al-Biruni, 7 :13-22

G. Xiong, U. Pal, J. Serrano, K. Ucer and R. Williams.

Photoluminesence and FTIR study of ZnO nanoparticles: the impurity and defect perspective, physica status solidi c, 3 :3577-3581

I. Machado, J. Graça, H. Lopes, S. Lopes and M.O. Pereira.2013. Antimicrobial pressure of ciprofloxacin and gentamicin on biofilm development by an endoscope-isolated Pseudomonas aeruginosa, ISRN biotechnology

Jayarambabu, N., Kumari, B. S., Rao, K. V., and Prabhu Y. T. 2014. Germination and growth characteristics of mungbean seeds (Vignaradiata L.) affected by synthesized zinc oxide nanoparticles. International Journal of Current Engineering and Technology, 4(5), 2347-5161

K. Shim, M. Abdellatif, E. Choi, D. Kim.2017. Nanostructured ZnO films on stainless steel are highly safe and effective for antimicrobial applications, Applied microbiology and biotechnology, 101: 2801-2809

L. Zhang, Y. Jiang, Y. Ding, N. Daskalakis, L. Jeuken, M. Povey, A.J. O’Neill and D.W. York.2010. Mechanistic investigation into antibacterial behaviour of suspensions of ZnO nanoparticles against E. coli, Journal of Nanoparticle Research, 12 :1625-1636

M. Abd Al-Mayali, E.D. Salman.2020. Bacteriological and Molecular Study of Fluoroquinolones Resistance in Pseudomonas aeruginosa Isolated From Different Clinical Sources, Iraqi Journal of Science, 2204-2214

N. Hussein.2019. Detection of the antibacterial activity of AgNPs biosynthesized by Pseudomonas aeruginosa, Iraqi Journal of Agricultural Science, 50 : 617-625

N.K. Abbas, A.A. Al-Attraqchi.2020. Antimicrobial Activities of Green Biosynthesized Iron Oxide Nanoparticles Using F. Carica Fruit Extract, Indian Journal of Forensic Medicine & Toxicology, 14 : 2181-2187

N. Al-Gbouri, A. Hamzah.2018. Evaluation of phyllanthus emblica extract as antibacterial and antibiofilm against biofilm formation bacteria, Iraqi Journal of Agricultural Sciences, 49:142-151

P.K. Stoimenov, R.L. Klinger, G.L. Marchin, K.J. Klabunde.2002. Metal oxide nanoparticles as bactericidal agents, Langmuir, 18 : 6679-6686

P. Edwards, andW. H. Ewing. 1972. Identification of Enterobacteriaceae, Burgess Publishing Co., J. CLIN. MICROBIOL. neonatal meningitis. J. Neurosurg, 52 : 547-552

R. Saravanan, V. Gupta, V. Narayanan, A. Stephen.2014. Visible light degradation of textile effluent using novel catalyst ZnO/γ- Mn2O3, Journal of the Taiwan Institute of Chemical Engineers, 45 :1910-1917

R. Salomoni, P. Léo, A. Montemor, B. Rinaldi and M. Rodrigues.2017. Antibacterial effect of silver nanoparticles in Pseudomonas aeruginosa, Nanotechnology, science and applications, 10 : 115.

R.P. Williams, C.L. Gott, J.A. Green.1961.STUDIES ON PIGMENTATION OF SERRATIA MARCESCENS V.: Accumulation of Pigment Fractions with Respect to Length of Incubation Time1, Journal of bacteriology, 81 :376

R. Facklam.1973. Comparison of several laboratory media for presumptive identification of enterococci and group D streptococci, Applied microbiology, 26 : 138-145

S. Azizi, M. Ahmad, M. Mahdavi, S. Abdolmohammadi.2013. Preparation, characterization, and antimicrobial activities of ZnO nanoparticles/cellulose nanocrystal nanocomposites, BioResources, 8 : 1841-1851

S.H. Korji.2012. Inhibition of Nitrate Reducutase Production from Gram-Negative Bacteria Using Zizyphus Spina-Christi Extract and Comparing with some Antibiotics, Iraqi Journal of Agricultural Science, 43 : 144-150

V. Kononenko, N. Repar, N. Marušič, B. Drašler, T. Romih and S. Hočevar, Drobne.2017. Comparative in vitro genotoxicity study of ZnO nanoparticles, ZnO macroparticles and ZnCl2 to MDCK kidney cells: Size matters, Toxicology in Vitro, 40 : 256-263

W.-C. Chen, W.-J. Yu, C.-C. Chang, J.-S. Chang, S.-H. Huang, C.-H. Chang, S.-Y. Chen, C.-C. Chien, C.-L. Yao and W.-M. Chen.2013. Enhancing production of prodigiosin from Serratia marcescens C3 by statistical experimental design and porous carrier addition strategy, Biochemical engineering journal, 78 : 93-100.

Downloads

Published

2022-02-23

Issue

Section

Articles

How to Cite

Alaa Alden, M. A. ., & Yaaqoob, L. A. . (2022). EVALUATION OF THE BIOLOGICAL EFFECT SYNTHESIZED ZINC OXIDE NANOPARTICLES ON PSEUDOMONAS AERUGINOSA. IRAQI JOURNAL OF AGRICULTURAL SCIENCES, 53(1), 27-37. https://doi.org/10.36103/ijas.v53i1.1502

Similar Articles

1-10 of 186

You may also start an advanced similarity search for this article.