EFFECT OF REPRODUCTIVE STAGES ON SOME BLOOD METABOLITES IN THE SHOW AND RACEHORSE MARES IN IRAQ

Authors

  • M. A. Abed
  • T. A. Abdulkareem
  • W. A. Saleh

DOI:

https://doi.org/10.36103/s2gw6m75

Keywords:

biomarkers, fatty acids, NEFAs, pregnancy, horses.

Abstract

This study explored the effect of different reproductive stages on some blood metabolic biomarkers of the show and racehorse mares in Iraq. We selected eighteen show mares and fifteen racehorse mares and divided them into three categories: last month of pregnancy, post-foaling, and first month of pregnancy. Blood samples were collected twice via jugular venipuncture, and blood biochemical metabolic biomarkers were evaluated by gas chromatography (GC) and high liquid chromatography (HPLC). A significant (P≤0.05) increase was observed for the concentration of serum glucose and creatine of pregnant show horse mares in the last month compared to the remaining stages. Serum fatty acid concentrations were numerically higher in the last month than in the remaining stages. Showhorse mares were higher than racehorse mares for all stages. The differences observed in serum non-esterified fatty acids for all stages and the show and racehorse mares lacked significance. The racehorse mares exhibited higher (P≤0.05) serum concentrations of total protein than those of the showhorse mares. For both types, serum total protein was higher (P≤0.05) in the first and last month than in the post-foaling period. In conclusion,  some blood metabolites differ according to reproductive stages and mare types and can considered for diagnosing diseases and determining nutritional requirements for each type and reproductive stage.

References

1. Abdulkareem, T. A., R. I. Khalil and M. A. O. Al-Ezzi. 2024. Effect of sperms freezability on some semen attributes and amino acids concentrations in seminal plasma of Holstein bulls. Iraqi J. Agric. Sci., 55 (2), 665-674. https://doi.org/10.36103/pfgrpt81

2. Abdulkareem, T.A., S. M. Eidan, A. M. H. Shubber, F. F. Ibrahim, M. D. Ali and O. A. Mohammed. 2020. Reference Physiological Values in Different Animal Species. Iraqi Media Network Publisher, pp. 66-67.

3. Abdullah, M. A. N., M. Ismail and E. M. Sari. 2021. Comparison of the reproductive performance of Gayo and Thoroughbred derivatives horses in Gayo Lues Regency. Advances in Biological Sciences Research, 20. Proceedings of the International Conference on Improving Tropical Animal Production for Food Security (ITAPS 2021), 159-162. https://doi.org/10.2991/absr.k.220309.033

4. Abeni, F., A. D. Prà, G. Bertin and L. Calamari. 2013. Serum protein fraction in mature horses and relationship with metabolic and hematological parameters. J. Equine Vet. Sci., 33, 905-911. https://doi.org/10.1016/j.jevs.2013.01.006

5. AL-Gebouri, F. G., S. M. Eidan. 2024. Metabolites and semen characteristics in different bulls fertility, Iraqi J. Agric. Sci. 55(Special Issue) : 206-216. https://doi.org/10.36103/ijas.v55iSpecial.1899

6. Alwaeli, S. N. and S. M. Eidan. 2024. Synergistic effect of serum filtration techniques and antioxidants added to extender of Iraqi buffalo semen. Iraqi. J. Agric. Sci., 55 (1), 413-421. https://doi.org/10.36103/kc809146

7. Ayad, A., O. Besseboua, S. Aissanou, M. Stefaniuk-Szmukier, K. Piórkowska, A. D. Musiat, B. Długosz, A. Kozłowska and K. Ropka-Molik. 2022. Profiling of genetic markers useful for breeding decision in Selle Francais horse. J. Equine Vet. Sci., 116, 104059. https://doi.org/10.1016/j.jevs.2022.104059

8. Barrelet, A. and S. Ricketts. 2002. Haematology and blood biochemistry in the horse: a guide to interpretation. In Practice, 24(6),318-327. https://doi.org/10.1136/inpract.24.6.318

9. Bis-Wencel, H., L. Saba, and M. Kowaleczko. 2009. Effect of recreational and therapeutic use of horse on chosen hematological parameters of half-bred saddle horse. Annales Universitatis Mariae Curie-Skłodowska. Sectio EE: Zootechnica, 27(2), 19-24.

10. Burlikowska, K., M. Bogusławska-tryk, R. Szymeczko, and A. Piotrowska. 2015. Haematological and biochemical blood parameters in horses used for sport and recreation. Journal of Central European Agriculture, 16 (4), 370-382. https://doi.org/10.5513/jcea.v16i4.3806

11. Ciesla, A., M. Pluta, and P. Maj, 2017. Horse behavior pattern at holiday time subject to its utilization form and morphological type. Acta Scientiarum Polonorum. Zootechnica, 16(4), 5-10. https://doi.org/10.21005/asp.2017.16.4.01

12. Davies Morel, M. C. G. 2022. Selection of the mare and stallion for breeding. In: Equine Reproductive Physiology, Breeding and Stud Management. Chapter 11, 4th edn, CABI Digital Library. pp.101-125. https://doi.org/10.1079/9781780644424.0101

13. Eidan, S. M., R. I. Khalil and A. F. Naser. 2024. Some fatty acids and semen characteristics of Holstein bulls as influenced by different sperm freezability. Iraqi J. Agric. Sci., 55 (2), 675-682. https://doi.org/10.36103/cg6xrf46

14. Fedorka, C.E., B.A. Ball, M.A. Wynn, M.E. McCormick, K.E. Scoggin, A. Esteller-Vico, T.E. Curry, L.A. Kennedy, E.L. Squires, and M.H. Troedsson. 2021. Alterations of circulating biomarkers during late term pregnancy complications in the horse part II: steroid hormones and alpha-fetoprotein. J. Equine Vet. Sci., 99, 103395. https://doi.org/10.1016/j.jevs.2021.103395

15. Galantino-Homer, H. L. J. B. Engiles. 2013. Insulin resistance and laminitis in broodmares. J. Equine Vet. Sci. 33(10), 844-846. http://dx.doi.org/10.1016/j.jevs.2013.08.011

16. Gibbs, P.G., G.D. Potter, B.D. Nielsen, D.D. Householder, and W. Moyer. 1995. Scientific principles for conditioning race and performance horses. The Professional Animal Scientist, 11(4), 195-203. https://doi.org/10.15232/S1080-7446(15)31903-3

17. Gündüz, M. C., G. Kaşikci and B. Ekis. 2008. Follicular and steroid hormone changes in Arabian mares in the postpartum period. Anim. Reprod. Sci., 109 (1-4), 200-205. https://doi.org/10.1016/j.anireprosci.2007.10.010

18. Hinchcliff, K.K., A. J. Kaneps and R. J. Geor. 2014. Exercise testing in the field. In: Equine Sports Medicine and Surgery. Chapter 3, 2nd edn., Saunders Elsevier: Philadelphia, PA, USA.

https://doi.org/10.1186/s41043-015-0018-9

19. Kabaran, S. and H. T. Besler. 2015. Do fatty acids affect fetal programming? J. Health Popul. Nutr., 33(1),1–9. https://doi.org/10.1186/s41043-015-0018-9

20. Kronfeld, D. S., K. H. Treiber and R. J. Geor. 2005. Comparison of nonspecific indications and quantitative methods for the assessment of insulin resistance in horses and ponies. J. Am. Vet. Med. Assoc. 226(5), 712-719. http://dx.doi.org/10.2460/javma.2005.226.712

21. Lawson, E.F., Grupen, C.G., Baker, M.A., Aitken, R.J., Swegen, A., Pollard, C.L. and Gibb, Z., 2022. Conception and early pregnancy in the mare: lipidomics the unexplored frontier. Reprod. Fertil., 3(1), R1-R18. https://doi.org/10.1530/RAF-21-0104

22. Masko, M., A. Chałabis-Mazurek, U. Sikorska, A. Ciesielska, Ł. Zdrojkowski and M. Domino. 2024. Monthly and pregnancy-related concentration of Cu and Zn in serum of mares in an equine breeding herd. Agriculture, 14 (1), 35. https://doi.org/10.3390/agriculture14010035

23. Meira, C.D., J.C. Ferreira, E.S.M. Silva, and F.S. Ignácio. 2012. Developmental aspects of early pregnancy in mares. Anim. Reprod., 9(3), 166-172.

24. Mousquer, M. A., A. B. Pereira, I. S. Finger, H. C. Franz, A. J. Torres, V. Muller and C. E. W. Nogueira. 2019. Glucose and insulin curve in pregnant mares and its relationship with clinical and biometric features of newborn foals. Pesq. Vet. Bras. 39(9), 764-770. https://doi.org/10.1590/1678-5150-PVB-6227

25. Musa. K. S. and T. A. Abdulkareem. 2024. Some biochemical attributes in seminal plasma of Iraqi buffalo bulls and their relation to the semen quality, Iraqi Journal of Agricultural Sciences. 55(1) : 402-412. https://doi.org/10.36103/nrfkex70

26. Nidl, C., J. O. Merlino, E. A. Lopez, A. E. Monreal, G. Aquirre, J. A. Rangel and C. Venegas. 2017. Effect of age, gender and season on hematological parameters in quarter horses. J. Vet. Sci. Med. Diagn., 6 (2), 1-4.

27. Nogueira, G. D. P., R. C. Barnabe, J. C. Bedran-de-castro, A. F. Moreira, W.R. Fernandes, R. M. S. Mirandola and D. L. Howard. 2002. Serum cortisol, lactate and creatinine concentrations in Thoroughbred fillies of different ages and states of training. Braz. J. vet. Res. Anim. Sci., São Paulo, 39 (1), 54-57.

28. Orr, C. 2016. Relationships Among Genetic and Physiological Biomarkers, and Horse Characteristics. University of Arkansas.

29. Piccione, G., F. Arfuso, F. Fazio, M. Bazzano, and C. Giannetto. 2014. Serum lipid modification related to exercise and polyunsaturated fatty acid supplementation in jumpers and thoroughbred horses. J. Equine Vet. Sci., 34(10), 1181-1187. https://doi.org/10.1016/j.jevs.2014.07.005

30. Popova, M., R. Malinova, V. Nikolov, B. Georgiev, P. Taushanova and M. Ivanova. 2020. Influence of the breed and age on hematological and biochemical indicators of mares from purebred from Arabian and East Bulgarian breeds. Scientific Papers. Series D. Animal Science, 63(1), 67-73.

31. Purohit, G.N. 2019. Intra-partum conditions and their management in mare. J. Livest. Sci, 2, 20-37.

32. Robyn, J., L. Plancke, B. Boshuizen, C. de Meeûs, M. de Bruijn and C. Delesalle. 2017. Substrate use in horses during exercise - the ‘fasted’ compared to the postprandial state. Vlaams Diergeneeskundig Tijdschrift, 2017, 86. https://doi.org/10.21825/vdt.v86i5.16166

33. SAS. 2012. Statistical Analysis System, User's Guide. Statistical. Version 9.1th ed. SAS. Inst. Inc. Cary. N.C. USA.

34. Silver, M. and Fowden, A.L. 1982. Uterine prostaglandin F metabolite production in relation to glucose availability in late pregnancy and a possible influence of diet on time of delivery in the mare. J. Reprod. Fert., 32 (Suppl.), 511-519 .

35. Swegen, A. 2021. Maternal recognition of pregnancy in the mare: does it exist and why do we care? Reproduction, 161 (6), R139-R155. https://doi.org/10.1530/REP-20-0437

36. Szarska, E. 2003. Investigations of blood parameters for evaluation of health status and training effects in race and sport horses. AR Wrocław, Poland, pp. 115.

37. Terblanche, H. M. and L. Maree. 1981. Plasma progesterone levels in the mare during the estrous cycle and pregnancy. J. S. Afr. Vet. Assoc., 52(3),181-185.

38. Wagner, L. H., J. Aurich, M. Melchert, C. T. C. Okada, C. Gautier, M. Caps, S. Claaßen and C. Aurich. 2023. Low progesterone concentration in early pregnancy is detrimental to conceptus development and pregnancy outcome in horses. Anim. Reprod. Sci., 257, 107334. https://doi.org/10.1016/j.anireprosci.2023.107334

39. WAHO. 2022. World Arabian Horse Organization. www.waho.org.

40. Wiles, K., K. Bramhame, P. T. Seed, C. Nelson-Piercy, L. Lightstone and L. C. Chappell. 2019. Serum creatinine in pregnancy: A systematic review. Kidney Int. Rep., 4, 408–419. https://doi.org/10.1016/j.ekir.2018.10.015.

41. Witkowska-Piłaszewicz, O., P. Baska, M. Czopowicz, M. Zmigrodzka, E. Szarska, J. Szczepaniak, Z. Nowak, A. Winnicka and A. Cywinska. 2019. Anti-inflammatory state in Arabian horses introduced to the endurance training. Animals 9, 616. https://doi.org/10.3390/ani9090616

42. Zeng, X., S. Li, L. Liu, S. Cai, Q. Ye, B. Xue, X. Wang, S. Zhang, F. Chen, C. Cai, F. Wang and X. Zeng. 2023. Role of functional fatty acids in modulation of reproductive potential in livestock. Journal of Animal Science and Biotechnology, 14, 24. https://doi.org/10.1186/s40104-022-00818-9

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Published

2025-01-30

How to Cite

M. A. Abed, T. A. Abdulkareem, & W. A. Saleh. (2025). EFFECT OF REPRODUCTIVE STAGES ON SOME BLOOD METABOLITES IN THE SHOW AND RACEHORSE MARES IN IRAQ. IRAQI JOURNAL OF AGRICULTURAL SCIENCES, 56(Special), 111-122. https://doi.org/10.36103/s2gw6m75

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