MOLECULAR CHARACTERIZATION OF M. PHASEOLINA AND ITS MANAGEMENT USING AGROCHEMICALS AND T. HARZIANUM
DOI:
https://doi.org/10.36103/4p59fb72Keywords:
chitosan, salicylic acid, M. phaseolina, T. harzianum , sunflower, PCRAbstract
Systemic Acquired Resistance (SAR) induced by agrochemicals of chitosan (CH) and salicylic acid (SA) at (25, 50 and 100 ppm) , in addition to a biocontrol agent of T. harzianum (Th) at 4 ×106 were examined against M. phaseolina the causal agent of charcoal rot of sunflower. The results depended on estimation of diseases severity and microsclerotia density in the soil. Thus, the seeds immersion in CH 75 ppm for 6 h., gave the highest and considerable reduction (p=0.05) in disease severity by 48.25% and reduced microsclerotia survived in the soil up to 70%. Application of SA at 50 and 75 ppm proved an obvious reduction of charcoal rot severity by up to 39% and 37% for both concentrations, respectively and not varied with Th. The results also confirmed that CH at 75 ppm revealed significant reduction 40.63% in disease severity and similarized with SA at same concentration. However, the lowest dose of SA at 25 ppm realized the highest reduction of micro sclerotia density by 80.28 % compared to 74.91% when used CH at 75 ppm. For molecular identification of a pathogen Polymerase Chain Reaction (PCR) using ITS4 and ITS5 universal primers were applied to amplify and sequence of DNA for six isolates of M. phaseolina viz., OL901219, OL636051, OL901220, OL901204, OL636050 and OL636053 compared for identity of rDNA sequence according to NCBI GenBank databases by BLAST mode and the results showed the entire similarity ratio reached to 100%
References
Aegerter B.J., T.R. Gordon and R.M. Davis 2000. Occurrence and pathogenicity of fungi associated with melon root rot and vine decline in California. Plant Disease 84, 224– 30. https://doi.org/10.1094/PDIS.2000.84.3.224
Alaa El-Den, H. R., G. M. Abd El-Wahab andS. A. Masoud. 2022. Using salicylic acid, folic acid and/or mancozeb in controlling tomato early blight biotic stress and their effects on growth, yield, fruit quality, and stress-related enzymes. Iraqi Journal of Agricultural Sciences, 53(6):1548-1559. https://doi.org/10.36103/ijas.v53i6.1670
Al-Atrushy, Sh. M. M. 2021. Effect of foliar application of zink and salicylic acid on vegetative growth and yield characteristic of halawani grape cultivar (Vitis vinifera L.) .Iraqi Journal of Agricultural Sciences. 52(4):989-998. https://doi.org/10.36103/ijas.v52i4.1410.
Aljassani, I. F., M. R. M. Alqaisi, and A. J. Al-Ahbabi 2022. Effect of the hydrogen peroxide and salicylic acid on induction the sod gene expression of date palm (Phoenix dactylifera l.) as defense factor against salinity stress. Iraqi Journal of Agricultural Sciences, 53(5):1099- 1106. https://doi.org/10.36103/ijas.v53i5.1622.
Al-Khafaji, A. M. H. H., and K. D. H. Al-Jubouri. 2023. Upgrading growth, yield, and folate levels of lettuce via salicylic acid and spirulina, vermicompost aqueous extracts, Iraqi Journal of Agricultural Sciences, 54(1):235-241. https://doi.org/10.36103/ijas.v54i1.1696
Arfan M., H.R. Athar and M. Ashraf 2007. Does exogenous application of salicylic acid through the rooting medium modulate growth and photosynthetic capacity in two differently adapted spring wheat cultivars under salt stress. J. Plant Physiol., 6(4): 685-694. • DOI: 10.1016/j.jplph.2006.05.010
Babu B. K., S. Mesapogu ,A. Sharma, S. R Somasani and D. K. Arora 2011 . Quantitative real-time PCR assay for rapid detection of plant and human pathogenic Macrophomina phaseolina from field and environmental samples. Mycologia 103, 466–473.
DOI: 10.3852/10-181
Babu B. K., A. K. Saxena A. K. Srivastava and D. K Arora 2007. Identification and detection of Macrophomina phaseolina by using speciesspecific oligonucleotide primers and probe. Mycologia 99, 797–803. DOI: 10.3852/mycologia.99.6.797
Bashasab R., and M. S. Kuruvinashetti 2007. Genetic variability of sorghum charcoal rot pathogen (Macrophomina phaseolina) assessed by random DNA markers. Plant Pathol. . 23, 45–50. DOI:10.5423/PPJ.2007.23.2.045
Bautista S., M. Hernández-López, E. Bosquez-Molina, and C. L. Wilson 2003 . Effects of chitosan and plant extracts on growth of Colletotrichum gloeosporioides, anthracnose levels and quality of papaya fruit. Crop Protection, 22(9):1087–1092. https://doi.org/10.1016/S0261-2194(03)00117-0
Benhamou N. and G. Theriault 1992. Treatment with chitosan enhances resistance of tomato plants to crown and root rot pathogen Fusarium oxyspornm f.sp. radices –Iycopersici. Physiological and Molecular Plant Pathology, 4:33-25. https://doi.org/10.1016/0885-5765(92)90047-Y
Bornet A. and P.L. Teissedre 2008. Chitosan, chitin and glucan and chitin effects on minerals (iron, lead, cadmium) and organic (ochratoxin A) contaminants in wines. European Food Research and Technology 226: 681-689. DOI 10.1007/s00217-007-0577-0
Bosch S.M., J. Penuelas and J. Llusia 2007. A deficiency in salicylic acid alters isoprenoid accumulation in water-stressed transgenic Arabidopsis plants. Plant Sci., 172(4): 756-762. DOI: 10.1016/j.plantsci.2006.12.005
Bressano M., M. L. Giachero, M. L.Celina and A. D. Daniel 2010. An in vitro method for examining infection of soybean roots by Macrophomina phaseolina. Physiological and Molecular Plant Pathology (74), 201-204. https://doi.org/10.1016/j.pmpp.2009.12.003
Chen Z., H. Silva and D. F. Klessing 1993. Active oxygen species in the induction of plant systemic acquired resistance by Salicylic acid. Sci 262: 1883-1885.
DOI: 10.1126/science.8266079
Cho's M.H., H.K. No's, and P. Witoon 2008. Chitosan Treatments Affect Growth and Selected Quality of Sunflower Sprouts. Journal of Food Science, Vol. 73, Issue 1, S70-S77. DOI: 10.1111/j.1750-3841.2007.00607.x
Cloud G. L., and J. C. Rupe 1991. Comparison of three media for enumeration of sclerotia of Macrophomina phaseolina. Plant Dis. 75:771-772. DOI: 10.1094/PD-75-0771
Delaney T. P., L. Friedrich and J. A. Ryals 1999. Arabidopsis signal transduction mutant defection in chemically and biologically induced disease resistance. Proceeding of the National Academy of Sciences USA, 92: 6602-6606. DOI:10.1073/pnas.92.14.6602
Delaney T. P., S. Uknes and B. Vernooij 1995. A center role of salicylic acid in plant disease resistance. Science, 266: 1247-1250. DOI: 10.1126/science.266.5188.1247
Dhingra O. D., and J. B. Sinclair 1978. Biology and Pathology of Macrophomina phaseolina. Minas Gerais: Universidade Federal de Viçosa: Impresa Universitaria. ID: 86210279
Durner J., J. Shah and D.F. Klessing 1997. Salicylic acid and disease resistance in plants. Trends in plant Science.2:266-274. https://doi.org/10.1016/S1360-1385(97)86349-2
Durrant W. E. and X. Dong, 2004. Systemic acquired resistance. Ann. Rev Phytopathol., 42:185-209.
DOI: 10.1146/annurev.phyto.42.040803.140421
Edmunds M. 1964. Eolid Mollusca from Jamaica, with descriptions of two new genera and three new species. Bulletin of Marine Science. 14:1-32. ID.285958
EI Ghaouth A., J. Arul and A. Asselin 1992. Antifungal activity of chitosan on two postharvest pathogens of strawberry fruits. Phytopathology, 28:398- 402.
DOI: 10.1094/Phyto-82-398
El- Hai K. M. A., M. El-Metwally, S. M. El-Baz and A. M. Zeid 2009. The Use of Antioxidants and Microelements for Controlling Damping-Off Caused by Rhizoctonia solani and Charcoal Rot Caused by Macrophomina phaseolina on Sunflower. Plant-Pathology-Journal-1812-5425.
DOI: 10.3923/ppj.2009.79.89
Gaddeyya G., P.S. Niharika, P. Bharathi and P.K.R. Kumar 2012. Isolation and identification of soil mycoflora in different crop fields at Salur Mandal. AdvAppl Sci Res., 3:2020-2026. ISSN: 0976-8610
Ghosh T., M. K. Biswas, C. Guin, and P. Roy 2018. A review on characterization, therapeutic approaches and pathogenesis of Macrophomina phaseolina. Plant Cell Biotechnol. Mol. Biol. 19, 72–84. ISSN: 0972-2025
Harman G.E. 2011. Multifunctional fungal plant symbionts: new tools to enhance plant growth and productivity. New Phytologist 189: 647- 649.
DOI: 10.1111/j.1469-8137.2010.03614.x
Hassan A. A., W. K. Ahmed and A. R. M. Al-Qaissic, 2020. Molecular Identification and biological control of Macrophomina phaseolina the causal agent of charcoal rot disease on sunflower plant. Proceedings of the 8th Scientific and 2nd International Conference on Agricultural Research. https://www.researchgate.net/publication/349830221
Hassan, A. A. and W. K. Ahmed 2015. Evaluation of compost and biological control efficiency agents in charchol rot disease caused by Macrophomina phaseolina (Tassi) codi on corn. Tikrit University Journal of Agricultural Sciences 15(3), 77 - 90. DOI:10.1007/s10327-016-0669-4
Hayat S, A. and B. A. Ahmad 2007.Salicylic acid: Biosynthesis, metabolism and physiological role in plants. A Plant Hormone. Springer, Dordrecht, pp 1-14. ID: 82786047
James C. 1971. A manual of Assessment Keys for Plant Diseases. Canada Dep. Agric., Pupl. 1458. ISBN: 0-89054-081-0
Jana T., T. R. Sharma, R. D. Prasad and D. K. Arora 2003. Molecular characterization of Macrophomina phaseolina and Fusarium species by a single primer RAPD technique. Microbiol. Res. 158, 249–257. 10.1078/0944-5013-00198
Javaid A. and R. Munir 2012. Bioassay Guided Fractionation of Withania Somnifera for the Management of Chickpea Blight Pathogen Ascochyta Rabiei. Int. J. Agric. Biol. 14: 797-800. WOS:000309210600019
Katiyar D., A. Hemantaranjan and B. Singh 2015. Chitosan as a promising natural compound to enhance potential physiological responses in plant: a review. Indian Journal of Plant Physiology. 20:1-9. DOI:10.1007/s40502-015-0139-6
Khan S.N. 2007. Macrophomina phaseolina as causal agent for charcoal rot of sunflower. Mycopathologia 5: 111–118. ID: 83222020
Khan W., P.Balakrishnan and D.L. Smith, 2003. Photosynthetic responses of corn and soybean to foliar application of salicylates. J. Plant Physiol., 160(5): 485-492.
DOI: 10.1078/0176-1617-00865
Kumar M.N.V.R. 2000 .A review of chitin and chitosan applications. Reactive and Functional Polymers 46: 1-27. http://dx.doi.org/10.1016/S1381-5148(00)00038-9
Lodha S. and R. Mawar 2019.Population dynamics of Macrophomina phaseolina in relation to disease management: A review. J. Phytopathol. 168:1–17. https://doi.org/10.1111/jph.12854
Lu, C., B. Song, H. Zhang, Y. Wang, and X. Zheng 2015. Rapid diagnosis of soybean seedling blight caused by Rhizoctonia solani and soybean charcoal rot caused by Macrophomina phaseolina using LAMP assays. Phytopa- thology 105:1612-1617. DOI: 10.1094/PHYTO-01-15-0023-R
Maleck, K., A. Levine and T. Eulgem 2000. The transcriptome of Arabidopsis thalrana during systemic ecquired resistance. Nature Genetics, 26: 403-410.
DOI: 10.1038/82521
Mauch-Mani B. and J.P. Metraux 1998. Salicylic acid and systemic acquired resistance to pathogen attack. Annals of Botany, 82: 535-540. https://doi.org/10.1006/anbo.1998.0726
Mayék-Pérez, N., C.López-Castañeda, M. González-Chavira , R. Garcia-Espinosa , J. Acosta-Gallegos, O.M. de la Vega and et al. 2001. Variability of Mexican isolates of Macrophomina phaseolina based on pathogenesis and AFLP genotype. Physiological and Molecular Plant Pathology 59:257-264. https://doi.org/10.1006/pmpp.2001.0361
Mengistu A., J. R. Smith, J. D. Ray and N. Bellaloui 2011. Seasonal progress of charcoal rot and its impact on soybean productivity. Plant Dis. 95:1159-1166. DOI: 10.1094/PDIS-02-11-0100
Mihail J. D. and S. M. Alcorn 1982. Quantitative recovery of Macrophomina phaseolina sclerotia from soil. Plant Dis. 66:662-663.
https://doi.org/10.1094/PD-66-662
Moharekar S. T., S. D. Lokhande , T. Hara , R. Tanaka , A. Tanaka and P. D. Chavan 2003.Effect of salicylic acid on chlorophyll and carotenoid contents of wheat and moong seedlings. Photosynthetica 41: 315-317. DOI:10.1023/B:PHOT.0000011970.62172.15
Mustafa, H.N, Al –Ogaidi I. 2023. Efficacy of zinc sulfide- chitosan nanoparticles against bacterial diabetic wound infection. Iraqi Journal of Agricultural Sciences, 54(1):1- 17. https://doi.org/10.36103/ijas.v54i1.1671
Ohta S., I. Ohsawa, K. Kamino, F. Ando and H. Shimokata 2004. Mitochondrial ALDH2 deficiency as an oxidative stress. Ann. NY Acad. Sci. 1011, 36– 44.
DOI: 10.1007/978-3-662-41088-2_4
Papavizas, G. C. and N. G. Klag 1975. Isolation and guantitative determination of Macrophomina phaseolina from soil. Phytopathology, 65: 182-187.
DOI: 10.1094/Phyto-65-182 .
Popova, L., T. Pancheva and A. Uzunova 1997 .Salicylic acid: Properties, Biosynthesis and Physiological role.Bul G. J. Physiol .23(1-2):85-93. ID: 18553457
Rad, Z. M., H. Nourafcan1, N. Mohebalipour, A. Assadi, and S. Jamshidi 2021. Effect of salicylic acid foliar application on phytochemical composition, antioxidant and antimicrobial activity of Silybum marianum. Iraqi Journal of Agricultural Sciences, 52(1):63-69. https://doi.org/10.36103/ijas.v52i1.1236
Ramamoorthy V., R. Viswanathan, T. Raguchander, V. Prakasam and R. Samiyappan 2001. Induction of systemic resistance by plant growth promoting rhizobacteria in crop plants against pests and diseases. Crop Protection 20: 1-11. https://doi.org/10.1016/S0261-2194(00)00056-9
Ramezani R., M. Ali, R. Hassan, E. Hamid and K. Masoumeh 2008. The effect of rosa damascena essential oil on the amygdala electrical kindling seizures in rat. Pakistan Journal of Biological Sciences, 11: 746-751. DOI: 10.3923/pjbs.2008.746.751
Ramírez M.A., A.T. Rodriguez, L. Alfonso and C. Peniche 2010. Chitin and its derivatives as biopolymers with potential agricultural applications. Biotecnologia Aplicada 27: 270-276.
ISSN 1027-2852
Raskin I. 1992a. Role of salicylic inplants. Annu. Rev. Plant. Physiology. 43:439-463. http://dx.doi.org/10.1146/annurev.pp.43.060192.002255
Raskin I. 1992.b. Salicylate a new plant hormone. Plant physiology. 99:799-803. doi: 10.1104/pp.99.3.799
Rayatpanah S., S. Nanagulyan , S. Alavi , E. Yasari , A. Prof , O. Carvil , et al. 2009. Phenotypic ariations of isolates of Macrophomina phaseolina from different hosts in Northern Iran. Austr. . Basic ppl. Sci. 3, 2908–2913. https://www.researchgate.net/publication/286782050
Reddy P.L.N., B.S. Babu, A. Radhaiah and A. Sreeramulu 2014. Screening, identification and isolation of cellulolytic fungi from soils of Chittoor District. India. Int J Curr Microbiol Appl Sci., 3: 761-771.
ID: 208726989
Rivas-San Vicente M. and J. Plasencia 2011. Salicylic acid beyond defence: its role in plant growth and development. Journal of Experimental Botany 62 (10): 3321-3338. DOI: 10.1093/jxb/err031
Saleh, A., Ahmed, H., Todd, T., Travers, S., Zeller, K., Leslie, J. and et al. 2010. Relatedness of Macrophomina phaseolina isolates from tallgrass prairie, maize, soybean and sorghum. Mol. Ecol. 19, 79–91. DOI: 10.1111/j.1365-294X.2009.04433.x
Shakirova F. M., A. R. Sakhabutdinov, D. R. Fatkhutdinova and M. V. Bezrukova 2003. Alicylic acid prevents the damaging action of stress factors on wheat plants. Bulg. J. PLANT Physiol., Special Issue , 314–319. ID: 5911401
Srivastava, A.K., T. Singh, T.K. Jana and D.K. Arora 2001. Microbial colonization of Macrophomina phaseolina and suppression of charcoal rot of chickpea. In Microbes and Plants; Sinha, A., Ed.; Vedamse Books (P) Ltd.: New Delhi, India, pp. 269–319. https://doi.org/10.1079/cabicompendium.32134
Sticher L., M. Mauch and J. P. Band Metraux 1997. Systemic Acquired Resistance. Annual Review of phytology, 35: 235 – 270. DOI: 10.1146/annurev.phyto.35.1.235
Su G., S.O. Suh, R.W. Schneider and J.S. Rusin 2001. Host specialization in the charcoal rot fungus, Macrophomina phaseolina. Phytopathology 92:120–126.
DOI: 10.1094/PHYTO.2001.91.2.120
Sundanshan, N.R., Ho over D.G. and Knorr D. 1992. Antibacterial action of chitosan. Food Biotechnology, 6:257-272. http://dx.doi.org/10.1080/08905439209549838
Taiz L. and E. Zeiger 2002. Plant Physiology. Sunderland, MA: Sinauer. pp 690. doi:10.1093/aob/mcg079
Uzunova A. N. and L. P. Popova 2000. Effect of salicylic acid on leaf anatomy and chloroplast ultrastructure of barley plants. Photosynthetica, 38:243- 250. DOI: 10.1023/A:1007226116925
Vandemark G., O. Martnez, V. Pecina and M.J. Alvarado 2000. Assessment of genetic relationships among isolates of Macrophomina phaseolina technique and two different methods of analysis. Mycologia, 92:656–664. http://dx.doi.org/10.4067/S0718-58392012000100007
Wagan, K.H., M.I. Khaskheli, J.D. Hajano and A.G. Lanjar 2019. Population density and aggressiveness of Macrophomina phaseolina isolates from Sindh, Pakistan. Sarhad Journal of Agriculture, 35(2): 400-407.
DOI | http://dx.doi.org/10.17582/journal.sja/ 2019/ 35. 2. 400.407
White, T.J. 1990. Amplification and Direct Sequencing of Fungal Ribosomal RNA Genes for Phylogenetics. In: PCR Protocols, a Guide to Methods and Applications, 315-322. https://www.scirp.org/reference/ReferencesPapers?ReferenceID=1356194
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