formulation diet, molecular technology, nutrition, optimum growth.


Traditional eel farmers face the problem of poor quality and high price of commercial glass eel diet, this feed has produced poor fish performance, as well. The lack of essential amino acids may be the cause. Molecular methods are used to determine the rapid response of glass eels to a formulated diet. The purpose of this study was to determine how the effect of adding lysine to commercial feed affects the expression of genes related to growth hormone GH. Pasta feed was given to glass eels (1600 heads) which were cultured for 60 days. The cylindrical plastic ponds were framed with steel and covered with HDPE plastic (300μm) in size {(3.14cm) x (85cmx85cm) x (60cm)} filled with water 907,460 L ≈ 0.91 m3. Real time RT-PCR results in GH suggest that the level of lysine (2%) added to the diet has strong effect on GH gene expression during eight weeks cultivation.


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Aksnes, A., B., Hope, E., Jönsson, B.T., Björnsson, and S., Albrektsen, 2006. Size-fractionated fish hydrolysate as feed ingredient for rainbow trout (Oncorhynchus mykiss) fed high plant protein diets. I: growth, growth regulation and feed utilization. Aquaculture: 261 (1), 305–317

Alam, M.S., S., Teshima, S., Koshio, M., Ishikawa, O., Uyan, L.H.H., Hector, and R.M., Fady, 2005. Supplemental effects of coated methionine and/or lysine to soy protein isolate diet for juvenile kuruma shrimp, Marsupenaeus Japonicus. Aquacult. 248, 13–19

Al-Barzinji, Y. M. 2022. Molecular analysis of fecgh gene in hamdani sheep breed in iraqi kurdistan region. Iraqi Journal of Agricultural Sciences, 53(1), 1-8.

Al-Azzawy, M. A. N., and M. S. Al-Khshali, 2018. Relationship of growth hormone gene with some of productive traits of common carp Cyprinus carpio. L. Iraqi Journal of Agricultural Science, 49(6), 1011-1017.

Alissianto, Y. R., Z. A., Sandriani, and B. S. Rahardja, 2018, April. The effect of amino acid lysine and methionine addition on feed toward the growth and retention on mud crab (Scylla serrata). In IOP Conference Series: Earth and Environmental Science (Vol. 137, No. 1, p. 012059). IOP Publishing.

Aristasari, E., R. A., Nur‘Aini, W., Nopita, M., Lamid, and M. A. Al-Arif, 2020, February. The growth, protein content, and fatty acid of catfish meat (pangasius sp.) With the addition of different lysine doses in commercial feed. In IOP Conference Series: Earth and Environmental Science (Vol. 441, No. 1, p. 012018). IOP Publishing.

Bicudo, Á.J.A., Sado, R.Y., Cyrino, J.E.P., 2009. Dietary lysine requirement of juvenile pacu Piaractus mesopotamicus (Holmberg, 1887). Aquaculture 297, 151–156

Brown, M. E. 1957. Experimental Studies on Growth in Fish Physiology, M. E. Brown (Ed). New York, Acad. Press, 1: 361-400. 8.Collins, M.D. and G.R Gibson. 199

Cao H, RJ, Zhou QW, Wei CG, Li and JF. Gui 2011. Molecular characterization of the growth hormone in Chinese sturgeon and its expression during embryogenesis and early larval stages. J Appl Ichthyol. 27:501–504

Chen, D., J., Liu, W., Chen, S., Shi, W., Zhang, and L. Zhang, 2015. Expression and Ontogeny of Growth Hormone (GH) in The Protogynous Hermaphroditic Ricefield Eel (Monopterus albus). Fish Physiol Biochem. 41 : 1515–1525

De Jesus EGT, Ayson FG, Amemiya Y, Hyodo S, Hirano T, Kawauchi H. 2002. Milk fish (chanos chanos) growth hormone cDNA cloning and mRNA expression in embryo and early larval stages. Aquaculture. 208:177–188.

Degani, G., I. Tzchori., S. Yom Din., D. Goldberg. and K. Jackson. 2003. Growth differences and growth hormone expression in male and female European Eels [Anguilla Anguilla (L.)]. General and Comparative Endocrinology Academic Press, 134: 88-93

Elsahookie, M. M., S. H., Cheyed, and A. A. Dawood, 2021. An overview on mechanism of gene expression regulation. Iraqi Journal of Agricultural Sciences, 52(2), 454-460.

Farah, K., I. R., Gunawan, G. B., Putra, W. P., Lokapirnasari, M., Lamid, E. D., Masithah, and T. Nurhajati, 2018, April. Effect of earthworm (Lumbricus rubellus) in feed formulation to improve fatty acids profile in eel (Anguilla bicolor) meat. In IOP Conference Series: Earth and Environmental Science (Vol. 137, No. 1, p. 012032). IOP Publishing.

Hassaana, M.S., A.I.M. El-Sayedb, M.A. Soltanb, M.M. Iraqib, A.M. Godaa, S.J. Daviesc, E.R. El-Haround, and H.A. Ramadan. 2019. Partial dietary fish meal replacement with cotton seed meal and supplementation with exogenous protease alters growth, feed performance, hematological indices and associated gene expression markers (GH, IGF-I) for Nile tilapia, Oreochromis niloticus

Jatmiko, P. C., Madinah, N. A., & Nurhajati, T. 2018, April. The effect of earthworms (Lumbricus rubellus) in feed formulation on growth and retention of eel (Anguilla bicolor). In IOP Conference Series: Earth and Environmental Science (Vol. 137, No. 1, p. 012033). IOP Publishing.

Khalida, A., N. I. D. N., Agustono, and W. P. Lokapirnasari, 2017. Penambahan lisin pada pakan komersial terhadap retensi protein dan retensi energi ikan bawal air tawar (Colossoma Macropomum). Jurnal Ilmiah Perikanan Dan Kelautan, 9(2), 98-106

Kamal, N.S.M .2022. Effect of dietary protected lysine supplementation on milk yield, composition and some blood and rumen parameters in local ewes. Iraqi Journal of Agricultural Sciences, 53(6), 1317-1324.

Li WS, Chen D, AO, Wohg and HR. Lin 2005. Molecular cloning, tissue distribution, and ontogeny of mRNA expression of growth hormone in orange-spotted grouper (Epinephelus coioides) Gen Comp Endocrinol. 2005;144:78–89. [PubMed] [Google Scholar]

Lestari, P., Suprapto, D., & Mahasri, G. (2020, February). Isolation and identification of bacteria in gastrointestinal of eel (Anguilla bicolor) that has potential as probiotic. In IOP Conference Series: Earth and Environmental Science (Vol. 441, No. 1, p. 012148). IOP Publishing.

Mustafa, S. A., A. J., Al-Rudainy, and J. K. Al-Faragi, 2019. Assessment of hydrogen peroxide on histopathology and survival rate in common carp, Cyprinus carpio L. infected with saprolegniasis. Iraqi Journal of Agricultural Science, 50(2), 697-704.

Ozaki, Y., H., Fukada, H., Tanaka, H., Kagawa, H., Ohta, S., Adachi, A., Hara, and K.Yamauchi, 2006. Expression of Growth Hormone Family and Growth Hormone Receptor During Early Development in The Japanese Eel (Anguilla Japonica). Comparative Biochemistry and Physiology. 145 : 27–34

Sukardi, P., N.A., Prayogo, T., Winanto, .S., Siregar, and T. AHarisam, 2018. Nursery I: The effect of stocking density on the performance of glass eels, Anguilla bicolor in the biofloc system. E3S Web of Conferences, 47, 02009

Setiyawan, D., S. H., Samara, and M. A. A. Arif, 2019, February. Effect of lysine in addition to commercial feed on crude protein and the energy digestibility of gourami (Osphronemus gouramy). In IOP Conference Series: Earth and Environmental Science (Vol. 236, No. 1, p. 012067). IOP Publishing.

Tantri, A. F., B. S., Rahardja, and A. Agustono, 2019. Penambahan lisin pada pakan komersial terhadap retensi protein dan retensi energi udang galah (macrobrachium rosenbergii). Journal of Aquaculture and Fish Health, 5(2), 78–84.

Zhou, C. P., B. Liu, J. Xie, X. P. Ge, P. Xu, Q. L. Zhou, L. K. Pan, and R. L. Chen. 2013. Effect of dietary carbohydrate level on growth performance, blood chemistry, hepatic enzyme activity, and growth hormone gene expression in Wuchang bream (Megalobrama amblycephala). Journal of Aquaculture - Bamidgeh, IJA_65.2013.882, 8 pages

Zhong, H., Y., Zhou, S., Liu, M., Tao, Y., Long, Z., Liu, C., Zhang, W., Duan, J., Hu, C., Song, and Y. Liu, 2012. Elevated Expressions of GH/IGF axis genes in triploid crucian carp. General and Comparative Endocrinology. 178 : 291–300.




How to Cite

Norman Arie Prayogo, Slamet Budi Prayitno, Tjahyo Winanto, Taufan Harisam, Ren Fitriadi, Baruna Kusuma and Purnama Sukardi (2023) “LYSINE REQUIREMENT IN THE DIET OF GLASS EELS, ANGUILLA BICOLOR, AGAINST GENE EXPRESSION ASSOCIATED WITH GROWTH HORMONE ”, IRAQI JOURNAL OF AGRICULTURAL SCIENCES, 54(4), pp. 1125–1130. doi: 10.36103/ijas.v54i4.1805.