EVALUTION OF THE BEST VACCINAL ROUTES AGAINST NEWCASTLE IN THE PRODUCTION STAGE OF LAYING HENS
DOI:
https://doi.org/10.36103/ijas.v54i3.1757Keywords:
ISSA brown, NDV, IgG, IgA, IFN-γ, ELISA.Abstract
Newcastle disease (ND) is a paramyxovirus-based infectious, highly contagious, and pathogenic avian viral disease. Despite the widespread use of ND vaccinations, ND remains a danger to poultry breeders worldwide. The specific goal of this study was to identify the best vaccination route against ND in the layer hens at production stage following oily vaccine. One hundred chickens at 30 weeks of age were collected from layer flocks (ISSA brown) and randomly divided into four groups. The groups received the following vaccinations: G1: Chicks were vaccinated two doses against ND by (La Sota strain) through drinking water at (30 and 40) weeks. G2: Chicks were vaccinated two doses against ND by (La Sota strain) through cross spray at (30 and 40) weeks. G3: Chicks were vaccinated two doses against ND by (La Sota strain) through intraocular at (30 and 40) weeks. G4: Chicks were not vaccinated and consider as control group. All groups challenge with virulent Newcastle virus isolates in a dose ELD50 105 at 50 weeks. To measure the (IgG, IgA, and IFN-γ) against ND, blood samples were taken at 35, 45, and 55 weeks of age. According to the results of this experiment, the third group, followed by the second group, produced the highest mean (IgG, IgA, and IFN-γ) titres among the vaccinated groups, while the first group produced the lowest titres when compared to the control negative (fourth) group, which recorded the lowest immune response and highly decrease in eggs production. The results were showed that intraocular vaccination with a live vaccine provides layer hens with a higher level of homogenous protection against vvNDV than spraying or drinking water vaccination.
References
Abdulwahid, M. T. 2015. Effect of injection hatching eggs with Newcastle disease vaccine and different doses of vitamin E on some productive traits and immune response of broilers. The Iraqi Journal of Veterinary Medicine. 39(2): 98–107
Ahmed, A.I. and M. Odisho. 2018. Isolation identification and pathotyping of Newcastle disease virus form naturally infected chickens in Iraqi Kurdistan region. Iraqi Journal of Agricultural Sciences. 49(1):132-141
Ahmed, K.A., V.K. Saxena, A. Ara, K.B. Singh, N.R. Sundaresan, M. Saxena and T.J. Rasool. 2007. Immune response to Newcastle disease virus in chicken lines divergently selected for cutaneous hypersensitivity. Int. J. Immun. 34 (5): 445–455
Al-Azawy, A. K. 2019. Isolation and Identification of Wild Isolate of Newcastle Disease Virus from Broiler Farm in Diyala Province: Virological and Histopathological Study: Amer Khazaal Al-Azawy, Karim Sadun Al-Ajeeli and Alaa Ismail. The Iraqi Journal of Veterinary Medicine, 42(2): 41–49
Alazzawi, R.I. and E. J. Khammas. 2021. Efficacy of oregano oil, citrus oil, and digestrom® P.E.P. on Newcastle disease infection in broilers. Iraqi Journal of Agricultural Sciences. 53(3):598-603
Bwala, D. G., S. Clift, N. M. Duncan, S. P. R. Bisschop and F. F. Oludayo. 2012. Determination of the distribution of lentogenic vaccine and virulent Newcastle disease virus antigen in the oviduct of SPF and commercial hen using immunohistochemistry. Research In Vet. Sci. 93(4): 520–528
Dalgaard, T.S., L.R. Norup, A.R. Pedersen, K.J. Handberg, P.H. Jorgensen and H.R. Juul-Madsen. 2010. Flow cytometric assessment of chicken T cell-mediated immune responses after Newcastle disease virus vaccination and challenge Vaccine. 28(2): 4506-4514
de Wit, J.J. and J.K. Cook. 2014. Factors influencing the outcome of infectious bronchitis vaccination and challenge experiments. Avian Pathol. 43(5): 485-497
Eze, C. P., J. O. A. Okoye, I. O. Ogbonna, W. S. Ezema, D. C. Eze, E. C. Okwor and E.A. Salihu. 2014. Comparative study of the pathology and pathogenesis of a local velogenic Newcastle disease virus infection in ducks and chickens. International Journal of Poultry Science. 13(1): 52–61
Ezema, W. S., J. O. A. Okoye and J. A. Nwanta. 2009. La Sota vaccination may not protect against the lesions of velogenic Newcastle disease in chicken. Tropical Animal Health and Production, 41(6): 474–477
Igwe, A. O., J. I. Ihedioha and J. O. A. Okoye. 2018. Changes in serum calcium and phosphorus levels and their relationship to egg production in laying hens infected with velogenic Newcastle disease virus. Journal of Applied Animal Research. 46(1): 523–528
Irena, L., H. Mazija, M. Šimpraga, I. Štoković, A. Tajana and V. Aleksandar. 2008. Effects of various application routes of Newcastle disease vaccine on specific antibody titres in ostriches. Acta. Vet. 58(2-3): 159-165
Kafi, M.A., M. B. Rahman, M.M. Amin, M.R. Islam, M.M. Rahman and M.K. Rahman. 2003. Comparative serological responses and protection conferred by vaccination with V4HR and BCRDV in chicken. Bang. J. Vet. Med. 1(1): 25-27
Khalifeh, M., M. Amawi, E. Abu-Basha, I.B. Yonis. 2009. Assessment of humoral and cellular-mediated immune response in chickens treated with tilmicosin, forfenicol, or enrofoxacin at the time of Newcastle disease vaccination. Poult. Sci. 88(4):2118–24
Miller, P. J., C. I. Afonso, J. E. Attrache, K. M. Dorsey, S. C. Courtney, Z. Guo and D. R. Kapczynski. 2013. Effect of Newcastle disease virus vaccine antibodies on the shedding and transmission of challenge virus. Developmental and Comparative Immunology. 41(4): 505–513
Munir, K., M. A. Muneer, A. Tiwari, R. M. Chaudhry and S. Muruganandan. 2007. Effects of polyether ionophores on the protective immune responses of broiler chickens against Angara disease and Newcastle disease viruses. Vet. Res. Commun. 31(6): 909–929
Mushtaq, T.B., H.A. Sahar, H.S. Raed and A.A. Amer 2017. Isolation and identification of the Newcastle disease virus from field outbreaks in broiler and layer flocks in Iraq. The Iraqi Journal of Veterinary Medicine. 41(1):23-27
Mustafa, M.M., F. Karadas and I.T. Tayeb. 2021. Adding different levels of turmeric powder and curcumin in the diet on broiler performance, carcass traits, immunity and gut morphology of broiler chicken under normal and heat stress condition. Iraqi Journal of Agricultural Sciences. 52(2):512-526
Okoroafor, O. N., P. C. Eze, W. S. Ezema, C. Nwosu, C. Okorie-Kanu, P. C. Animoke and J. O. A. Okoye. 2018. La Sota vaccination may not protect against virus shedding and lesions of velogenic viscerotropic Newcastle disease in commercial turkeys. Tropical Animal Health and Production. 50(2): 345–351
Rue, C.A., L. Susta, I. Cornax, C.C. Brown, D.R. Kapczynski, D.L. Suarez, D.J. King, P.J. Miller and C.L. Afonso. 2011. Virulent Newcastle disease virus elicits a strong innate immune response in chickens. J. Gen. Virol. 92(20): 931–939
Sae Silva, M. S., L. Susta, K. Moresco and D. E. Swayne. 2016. Vaccination of chickens decreased Newcastle disease virus contamination in eggs. Avian Path. 45(8):38–45
Salam, R., A. Aslam, S.A. Khan, K. Saeed and G. Saleem. 2003. Effect of different routes of vaccination against Newcastle disease on lymphoid organs of broilers. Pakistan Vet. J. 23(2): 78 -83
SAS. 2012. Statistical Analysis System, User's Guide. Statistical. Version 9.1th ed. SAS. Inst. Inc. Cary. N.C. USA
Sasipreeyajan, J. and N. Chansiripornchai. 2007. Comparative efficacy of various routes of vaccination of live C -2 strain Newcastle disease vaccine in chickens with no Newcastle disease antibodies. J Thai Vet Med Assoc. 58(3): 48 -57
Sasipreeyajan, J., T. Pohuang and N. Sirikobkul. 2012. Efficacy of different vaccination programs against Newcastle disease virus challenge in broiler chickens. Thai. J. Vet. Med. 42(4): 431 -437
Sharma, J.M. S. Rautenslein, D.E. Swayne, J.R. Glisson, L.R. McDougald, L.K. Nolan, D.L. Suarez and V.L. Nair. 2013. Diseases of Poultry (13th ed.), Host factors for D. R. Blackwell Publishing Ltd., Ames. IA. 22(4):61-68
Tizard, I.R. 2013. Veterinary immunology. 9th ed. St. Louis: Elsevier. 34(12): 551-559
Türker, S., E. Onur and Y. Ozer. 2004. Nasal route and drug delivery systems. Pharm World Sci. 26(3): 137–142
Ugwoke, M.I., R.U. Agu, N. Verbeke and R. Kinget. 2005. Nasal mucoadhesive drug delivery: Background, applications, trends and future perspectives. Adv. Drug Deliv. Rev. 57: 1640–1665
Waheed, U., M. Siddique, M. Arshad, M. Ali and A. Saeed. 2013. Preparation of Newcastle disease vaccine from VG/GA strain and its evaluation in commercial broiler chicks. Pakistan J. Zool. 45(11):339–44
Zeljko, C., N. Gordana, J. Mladen, B. Kreso and G. M. Hrvoje, 2021. Immunogenicity of Newcastle disease virus strain ZG 1999 HDS applied oculonasally or by means of nebulization to day-old chicks. Vol. 100, Iss. 4, Poult. Sci. 100(22):101-112.
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