ROLE OF KAOLIN ON HEMTOLOGICAL, BIOCHEMICAL AND SURVIVAL RATE OF CYPRINUS CARPIO CHALLENGED WITH PESUYDOMONAS AERUGINOSA
DOI:
https://doi.org/10.36103/ijas.v54i2.1723Keywords:
Fish, Blood, Total protein, MortalityAbstract
In present study, we assessed the role of kaolin [(Al2Si2O5(OH)4), an inert clay], for treatment of common carp, Cyprinus carpio experimentally infected with Pesudomonas aeruginosa. Fish were experimentally challenged with P. aeruginosa (LD50= 2±0.2×109 CFU/ml), in untreated water or water treated with addition of kaolin at levels of 4, 6 and 8g/l (G1, G2 and G3 respectively). Over the 5‐day course of kaolin treatment led to significantly (P ≤ 0.05) improved of survival (100%) in treated groups (G2 and G3) as compared to untreated fish (infected group; 75% survival). Considerable changes were observed in blood parameters, leucocyte count recorded significant increase (P≤0.05) in G2 and G3 relative to C- and C+ groups. Erythrocyte count, Hb content and HT(%) reported significant decrease (P≤0.05) in all treated groups (G1, G2 and G3) related to C- group. Globulin level registered significantly increased (P≤0.05) in G2 and G3 than C+ group. The current study strongly recommends that treatment of common carp infected with kaolin at 6 and 8g/l improves survival, hematological and biochemical profile, against P. aeruginosa. Future complete molecular studies are required before application of kaolin as a treatment in aquaculture.
References
Ackerman, U. 1992. Blood Proteins. Essential of Human Physiology. 1992. Churchhill Livingstone, New York, pp: 32–35
Agarwal, S.P., R. Khanna, R. Karmarkar, M.K. Anwer, and R.K. Shilajit. 2007. A review, Phytother. Res. 21: 401–405
Ajayi, T., L.R. Almond, T. Sawa, T. and J.P. Wienerkronish. 2003. Clinic Microbiology. 204p.
Al-Rudainy, A.J., S.A. Mustafa and M.A. Abdulaziz. 2014. Toxic effects of mercuric chloride on DNA damage, hematological parameters and histopathological changes in common carp, Cyprinus carpio. Iraq J. Vet Med. 38(2):87-94
Ardura, A., A. R. Linde and E. Garcia-Vazquez. 2013.Genetic detection of Pseudomonas spp. in commercial amazonian fish. Int. J. Environ. Res. Public Health. 10: 3954–3966.
Ashour, A. A., N. M. Salman, S. A. Mustafa and R. O. Nemah. 2019. Evaluation of hydrogen peroxide on controlling saprolegniasis in common carp, Cyprinus carpio L. Biochem. Cell. Arch. 19(2): 4247-4252
Barton, M.D. 2000. Antibiotic use in animal feed and its impact on human health. Nutr. Res. Rev. 13: 279–299
Beck, B. H., L. M. Barnett, B.D. Farmer, E. Peatman and D. Carter. 2015. Kaolinitic clay protects against Flavobacterium columnare infection in channel catfish Ictalurus punctatus (Rafinesque). J. Fish Dis. 38(3): 241–248
Boranic, M. 2000. What a Physician Should Know About Zeolites (in Croatian). LijecnickyVjesnik: 122: 292–298
Danabas, D.T. 2011. Effects of zeolite (Clinoptilolite) on some water and growth parameters of rainbow trout (Oncorhynchus mykiss Walbaum,1792). Dig. J. Nanomater. Biostruct. 6: 1111–1116
Divyagnaneswari, M., R. Christybapita and R.D. Michael. 2007. Enhancement of nonspecific immunity and disease resistance in Oreochromis mossambicus by Solanum trilobatum leaf fractions, Fish Shellfish Immunol. 23: 249–259
Dominy, N.J., E. Davoust and M. Minekus. 2004. Adaptive function of soil consumption: an in vitro study modelling the human stomach and small intestine. J. Exp. Biol. 207: 319–324
Doumas, B.T., W.A. Waston and H.G. Brigg,. 1971. Albumin standards and the measurement of serum albumin with bromocresol green, Clin. Chem. Acta. 31:87–96
Eissa I.A.M., H.I. Derwa, M.M. Ismail, M.M. El-Lamie and T.M. Elsayed. 2015. Use of kaolin for protection against flavobacteriosis in Oreochromis niloticus (SCVMJ, XX 1: 307-314
Eya, J.C., A. Parsons, J. Haile and P. Jagidi. 2008. Effects of dietary zeolites (bentonite and mordenite) on the performance Juvenile rainbow trout Onchorhynchus myskis. Austra. J. Basic App. Sci. 2(4): 961-967
Gram L., H.H. Huss. 2000. Fresh and Processed Fish and Shellfish. In: Lund B.M., Baird-Parker A.C., Gould G.W., editors. The Microbiological Safety and Quality of Foods. Chapman and Hall; London, UK. pp: 472–506.
Gram L., L. Ravn, M. Rasch, J.B. Bruhn, A.B. Christensen and M. Givskov. 2002. Food spoilage-interactions between food spoilage bacteria. Int. J. Food Microbiol. 78:79–97.
Jawahar, S., A. Nafar, K. Vasanth, M.S. Musthafa, J. Arockiaraj, C. Balasundaram and R. Harikrishnan. 2016. Dietary supplementation of zeolite on growth performance, immunological role, and disease resistance in Channa striatus against Aphanomyces invadans, Fish Shellfish Immunol. 51: 161–169
Kanyilmaz, M. and N. Tekellioğlu. 2016. Effects of dietary zeolite levels on some blood parameters of gilthead sea bream (Sparus aurata) juveniles, J. Aquacult. Engin. Fish. Res. 2: 119–127
Lamont, I. L. and L.W. Martin. 2003. Identification and characterization of novel pyoverdine synthesis genes in Pseudomonas aeruginosa. Microbiology. 149: 833–842
Lichenstein, H.S. D.E. Lyons, M.M. Wurfel, D.A. Johnson, M.D. McGinley, J.C. Leidli, D.B. Trollinger, J.P. Mayer, S.D. Wright and M.M. Zukowski. 1994. Afamin is a new member of the albumin, alpha-fetoprotein, and vitamin Dbinding protein gene family, J. Biol. Chem. 269: 18149–18154
Liu, M.Z., X.J. Leng, X.Q. Li, C.W. Xiao and D.R. Chen. 2011. Effects of azomite on growth performance, intestinal digestive enzyme activities and serum nonspecific immune of grass carp (Ctenopharyngodon idellus), J. Zhejiang Univ. 37: 312–318
Liu, A.J., X.J. Leng, X.Q. Li, L.P. Wang, Y.X. Luo and R.J. Zhu. 2009. Effects of azomite on growth, intestinal structure and non-specific immunity of tilapia (Oreochromis niloticus x O. aureus). China J. Anim. Nutri. 21: 1006–1011
Mac Faddin, J. F. 1985. Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria. (Williams and Wilkins, 1985).
Mizuno, S., Y. Sasaki , N. Omoto and K.Imada. 2004. Elimination of adhesiveness in the eggs of shishamo smelt Spirinchus lanceolatus using kaolin treatment to achieve high hatching rate in an environment with a high iron concentration. Aquaculture. 242: 713–726
Mustafa, S. A., S.J. Davies and A.N. Jha. 2012. Determination of hypoxia and dietary copper mediated sub-lethal toxicity in carp, Cyprinus carpio, at different levels of biological organisation. Chemosphere. 87(4): 413–422.
Mustafa, S. A., S.S. Karieb, S.J. Davies and A.N. Jha. 2015. Assessment of oxidative damage to DNA, transcriptional expression of key genes, lipid peroxidation and histopathological changes in carp Cyprinus carpio L. following exposure to chronic hypoxic and subsequent recovery in normoxic conditions. Mutagenesis. 30(1): 107–116.
Mustafa, S.A., A. J. Al-Rudainy. 2021. Impact of mercury chloride exposure on some of immunological and biochemical assays of common carp, Cyprinus carpio Iraqi J. Agric. Sci.52(3):547-551. https://doi.org/10.36103/ijas.v52i3.1341
Musthafa, M.S., A.R.J. Ali, A.R.H. Ali, M.J. Mohamed, M. War, M.S. Naveed,M.K. Al-Sadoon, B.A. Paray, K. Umaa Rani, J. Arockiaraj, C. Balasundaram, and R. Harikrishnan. 2016. Effect of shilajit enriched diet on immunity, antioxidants, and disease resistance in Macrobrachium rosenbergii (de Man) against Aeromonas hydrophila. Fish Shellfish Immunol. 57: 293–300
Nyenje M.E., C.E. Odjadjare, L. Odjadjare, N.F. Tanih, E. Green and R.N. Ndip. 2012. Foodborne pathogens recovered from ready-to-eat foods from roadside cafeterias and retail outlets in Alice, Eastern Cape Province, South Africa: Public Health Implications. Int. J. Environ. Res. Public Health. 9: 2608–2619.
Oyewo, E.B., M.A. Akanji, M.O. Iniaghe and P.B. Fakunle. 2012. Toxicological implications of aqueous leaf extract of Andrographis paniculata in wistar rat, Nat. Sci. 10: 91–108
Phillips, T.D. 1999. Dietary clay in the chemoprevention of aflatoxin-induced disease. Toxicolog. Sci. 52: 118–126
Roberts, R. J. 2012. Fish Pathology. Wiley, Blackwell , Hoboken.4th ed.83p.
Suhail, R. N., S.A. Mustafa and E. AL-Obodi. 2022. Efficiency of silver nanoparticles as antibacterial against Aeromonas hydrophila isolated from infected common carp. Iraqi J. Agric. Sci. 53(3): 589–597. https://doi.org/10.36103/ijas.v53i3.1568
Tan, C.G., X.Q. Li, X.J. Leng, X.G. Su, L. Chen, B. Liu, F. Ma, X.Q. Cai and T. Guo. 2014. Effects of supplemental azomite in diets on growth, immune function and disease resistance of white shrimp (Litopenaeus vannamei). Aquacult. Nutr. 20: 324–331.
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