ASSOCIATION BETWEEN ATP1A1 GENE POLYMORPHISMS WITH SEMEN CHARACTERISTICS IN HOLSTEIN BULLS
DOI:
https://doi.org/10.36103/ijas.v54i2.1706Keywords:
gene, sperms quality, bulls, covid-19 pandemic.Abstract
This study was conducted to identify the polymorphism of ATP1A1 gene, distribution ratios, and its association with semen characteristics of Holstein bulls. Twenty-five Holstein bulls were used currently. Blood samples were collected via venipuncture to separate the DNA and identify the polymorphism of the ATP1A1 gene. Semen was collected using an artificial vagina for fresh evaluation. Results were showed the change of cytosine (C) nucleotide at site 15738 to adenine (A) at the exon 17 (SNP C15738A) of the ATP1A1 gene. Two polymorphisms were obtained according to this change, the first is wild (CC; allele frequency 0.84), and the second is heterogeneous (CA; allele frequency 0.16). The CC polymorphism of the ATP1A1 gene exhibited a highly significant (p<0.01) superiority for ejaculate volume, sperm concentration, and mass activity, and for sperm’s cell individual motility (p<0.05), compared with the CA polymorphism of Holstein bulls. The other semen characteristics did not differ between the two mentioned polymorphisms. This polymorphism exhibited obvious semen characteristics despite stress conditions of feed shortage and poor management practices accompanied with the covid-19 pandemic. This gene can be used to predict the fertility and stress resistance of bulls before they reach sexual maturity and its reflection on the economic return of breeding these bulls at the artificial insemination centers.
References
Al-Dulaimi, M. Th. S. 2018. Relationship of Genetic Diversity of the Gene to Heat Shock and Seasonal Changes in Sexual Behavior and Sperm Semen Characteristics in Bulls. M.Sc. Thesis,College of Agriculture - University of Diyala
Al-Dulaimy, M.Th.S., S.M. Eidan and S.H.Al Azzawi. 2019. Genetic polymorphisms of heat shock protein gene (HSP90AA1)and association with characteristics of heat tolerance bulls. Biochem. Cell. Arch., 19(1): 1605-1611
AL-Nuaimi, A.J. and T.A. Abdulkareem. 2020. Effect of adding olea europaea and rosmarinus officinalis aqueous extracts and calcium chloride to Tris extender on post-cryopreservative sperms cell individual motility and live sperm percentage for low semen quality of Holstein bulls. Biochem. Cell. Arch. 20(1): 493-498
AL-Nuaimi, A.J., T.A. Abdulkareem, F.F. Ibrahim, Z.A. Humade and F.A. Hussein. 2020. Effect of adding olea europaea and rosmarinus officinalis aqueous extracts and calcium chloride to soybean lecithin extender on post-cryopreservative sperms abnormality percentage for low semen quality of Holstein bulls. Biochem. Cell. Arch. 20(1): 519-524
Al-Saedi, A.J.A., T. A. Abdulkareem .2022. Comparison of semen quality for three lines of Holstein bulls: 1. some immediate and microscopic characteristics. Iraqi Journal of Agricultural Sciences,53(4): 752-759. https://doi.org/10.36103/ijas.v53i4.1585
Bajagai, Y. S. 2011. Global climate change and its impacts on dairy cattle. Nepalese Vet. J., 30: 2-16
Barendse, W., A. Reverter, R.J. Bunch, B.E. Harrison, W. Barris and M.B. Thomas. 2007. A validated whole-genome association study of efficient food conversion in cattle. Genetics, 176(3): 1893-1905
Bearden, H. J., J. Fuquay and S.T. Willard. 2004. Semen and its components. In: Applied Animal Reproduction. 6th edn., Pearson Prentice Hall Publisher, pp. 173-182
Clarke,R.J., M. Catauro, H.H. Rasmussen and H-J. Apell. 2013. Quantitative calculation of the role of the Na+,K+-ATPase in thermogenesis. Biochimica et Biophysica Acta, 1827:1205–1212
Coulter, G.H and J.P. Kastelic. 1999. Management programme for developing bulls. In: J. L. Howard and R Smith, editors, Current Veterinary Therapy-Food Animal Practices 4. W.B. Saunders Co., pp: 127-136
Eidan, S.M., 2016. Effect on post-cryopreserved semen characteristics of Holstein bulls of adding combinations of vitamin C and either catalase or reduced glutathione to Tris extender. Animal Reproduction Science, 167:1-7
Eidan, S. M., F.F. Ibrahim, T.A. Abdulkareem and K.F. Hurish. 2017a. Effect of post foot and mouth disease infection on some semen attriutes and blood biochemical parameters of Holstein bulls. I.J.S.N., 8(3): 557-560
Eidan, S. M., R.I. Khalil and Z.H. Ali. 2017b. Effect of melatonin implantation on Semen quality Holstein bulls:1-Ejaculate volume and Total normal morphology sperm. Al-anbar Vet. Sci.,10(1): 145-153.
Hamad, A.W.R.,H.I. Al-Daghistani,W.D. Shquirat, M. Abdel-Dayem and M. Al-Swaifi. 2014. Sodium, potassium, calcium and copper levels in seminal plasma are associated with sperm quality in fertile and infertile men. Biochem pharmacol, 3(4):1-7
Han, Y. and F. Penagaricano. 2016. Unravelling the genomic architecture of bull fertility in Holstein cattle. BMC Genetics, 17: 143-154
Harchegani, B .A., A. Irandoost, M. Mirnamniha, H. Rahmani, E. Tahmasbpour and A. Shahriary. 2019. Possible mechanisms for the effects of calcium deficiency on male infertility. Int. J. Fertil. Steril., 12(4):267-272
Hassan M. S and S.M. Eidan. 2021. Effect of swim-up and glass wool techniques, with adding antioxidants to Tris extender on improving post cryopreserved some semen attributes of low semen quality of Holstein bulls. Iraqi Journal of Agricultural Sciences,52 52(3):552-563.https://doi.org/10.36103/ijas.v52i3.1342
Hassan, M. S., S.M. Eidan, F.F. Ibrahim and K.J. Yahya. 2021. Effect of swim-up and glass wool techniques, with adding antioxidants to tris extender on improving post cryopreserved total characteristics straw and freezability percent for low semen quality of Holstein bulls. Iraqi Journal of Agricultural Sciences,52(4):885-895.
Hoffman, J. F., A. Wickrema, O. Potapova, M. Milanick and D.R. Yingst. 2002. Na pump isoforms in human erythroid progenitor cells and mature erythrocytes. Proc. Natl. Acad. Sci. U.S.A. 99: 14572–14577.
Kashyap, N., P. Kumar, B. Deshmukh, S. Bhat, A. Kumar, A. Chauhan, B. Bhushan, G. Singh and D. Sharma. 2015. Association of ATP1A1 gene polymorphism with thermotolerance in Tharparkar and Vrindavani cattle. Veterinary World, 8(7): 892-897
Kaya,A., Birler,S., L. Enwell and E. Memmli. 2014. Determination of sperm morphology. In Animal Andrology: Theories and Applications by P.J. Chenoweth and S.P. Lorton.CAB International.pp:34-47
Landim-Alvarenga, F.C., J.K. Graham, M.A. Alvarenga and E.L. Squires. 2004. Calcium influx into equine and bovine spermatozoa during in vitro capacitation, Anim. Reprod.,1(1):96-105.
Leushuis, E. V. J.W. Steeg, P. Steures, S. Repping, P.M. Bossuyt and M.A. Blankenstein. 2010. Reproducibility and reliability of repeated semen analyses in male partners of sub-fertile couples. Fertil. Steril., 94: 2631–2635
Liu, Y.X., X. Zhou, D.Q. Li, Q.W. Cui and G.L. Wang.2010. Association of ATP1A1 gene polymorphism with heat tolerance traits in dairy cattle. Gen. Mol. Res., 9(2):891-896
Massanyi, P., R.Toman, J. Trandzik, P. Nad, M. Skalicka and B. Korenekova. 2004. Concentration of copper, zinc, iron, cadmium, lead and nickel in bull, ram, boar, stallion and fox semen. Trace Elements and Electrolytes, 21(1):45-49
Mohamed, O.A and T.A. Abdulkareem. 2020. Some post-cryopreserved semen characteristics of Holstein bulls as influenced by adding aquoeus extract of urtica dioica and date palm pollen powder to tris extender. Plant Archives, 20 (1):461-467
Mohamed, O.A. and T.A. Abdulkareem. 2020. Some post-cryopreserved semen characteristics of Holstein bulls as influenced by adding aqueous extract of urtica dioica and date palm pollen powder to tris extender. Plant Archives, 20(1):461-467.
Moura, A. A and E. Memili. 2016. Functional aspects of seminal plasma and sperm proteins and their potential as molecular markers of fertility. Anim. Reprod. 13:191-199
Pivovarov, A.S., F. Calahorro and R. Walker. 2019. Na+/K+ pump and neurotransmitter membrane receptors. Invertebrate Neuroscience , 19:1-16
Poulsen, H., F. Hilbers and M.V. Clausen. 2017. The Structure and Function of the Na,K-ATPase isoforms in health and disease. Frontiers in Physiology, 8: 371-387
Rahman, M.B . 2013. Stress responses in bovine spermatozoa and their consequences for in vitro fertilization. The thesis of Doctor of Philosophy (PhD) in Veterinary Sciences, Faculty of Veterinary Medicine, Ghent University. Belgium
Setchell, B.P. 2014. Semen and its constituents in: Animal Andrology:Theories and Applications by P.J.Chenoweth and S.P.Lorton.CAB International.pp:3-7
Singh,A.K., S.K. Rajak, P. Kumar, S. Kerketta and R.K. Yogi. 2018. Nutrition and bull fertility: A review. Journal of Entomology and Zoology Studies, 6(6): 635-643
Sultan, O.A.A and S.M. Eidan. 2020a. Association of CD9 gene with semen quality of Holstein bull : 1Fresh semen. Biochem. Cell. Arch., 20(1): 2721-2725
Sultan, O.A.A and S.M.Eidan. 2020b. Association of CD9 gene with semen quality of Holstein bull : 2-Post-cryopreservative semen. Biochem. Cell. Arch., 20( 1): 2697-2701.
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