BROILER GENOTYPING AND ALLILIC FREQUNCY ANALYSIS BY PCR-RFLP FOR IGF1 AND IGF2 GENES
DOI:
https://doi.org/10.36103/ijas.v53i5.1637Keywords:
HinfI, HinfI, Amino Acids.Abstract
This study was aimed to investigate Broiler chickens using PCR-RFLP (IGF1 and IGF2 genes). HinfI and Hsp92II restriction enzymes were used to amplify and digest 793-bp and 1146-bp fragments of the IGF1 gene, respectively. TT, TC, and CC genotypes were found in both genes in two different T and C allele subgroups. IGF1 had a higher prevalence of the C-allele (0.86) than IGF2, whereas IGF2 had a higher prevalence of the T-allele (0.5611).
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