EFFECT OF KAOLIN ON BIOCHEMICAL PARAMETERS AGAINST AEROMONAS HYDROPHILA IN CYPRINUS CARPIO
DOI:
https://doi.org/10.36103/5tch9p19Keywords:
albumin, carp, blood, total proteinAbstract
The present study aimed to evaluate the role of kaolin on hematological and biochemical profiles of common carp, Cyprinus carpio infected with Aeromonas hydrophila, A total of 180 of common carp were collected 40 g ; 60 fish were used to determine the median lethal dose LD50 of A. hydrophila, )LD50 was 1.57 × 106 CFU/ml) for 15 days. 120 fish were randomly distributed into six treatment groups (10 fish/treatment in duplicate) as follows: C- served as control negative group (without infection and treatment); C+ act as control positive group (infected with A. hydrophila but not treatment with Kaolin); T1, T2, T3 and T4 were intraperitoneally (IP) injected with A, hydrophila (at dose of 0.1 ml) and then treated with Kaolin at concentrations of 2, 4, 6 and 8 g/L respectively. In hematological indices, the experimental groups of carp (C+, T1, T2, T3 and T4) showed a significant (P<0.01) decrease in the number of erythrocytes, Hb content and PCV% compared to C- group. While, WBC numbers showed a significant (P<0.01) increase in all experimental carp groups relative to the C- group. As far as biochemical profile are concerned, the experimental groups of carp (C+, T2, T3 and T4) reported a significant (P<0.05) increase in total protein compared to C- group. In Globulin, Groups (T2, T3 and T4) showed a significant (P<0.01) increase compared to C- group. In conclusion, the treatment of common carp with Kaolin particularly at concentration of 8 g/L (T4) reduced the effect of A. hydrophila.
References
. Abdulathem, A. A. and A. J. Al-Rudainy. 2021. Effect of dietary zeolite on ammonia toxicity and on some of blood parameters in common carp Cyprinus carpio. Iraqi Journal of Agricultural Sciences, 52(5):1276–1283. https://doi.org/10.36103/ijas.v52i5.1465
Abdulathem, A.A. and A.J. Al-Rudainy. 2021. Effect of zeolite on ammonia toxicity and on some of blood parameters in common carp Cyprinus carpio. Iraqi Journal of Agricultural Sciences, (4): 918–924. https://doi.org/10.36103/ijas.v52i4.1400
Acharya, G. and P. K.Mohanty. 2018. Effect of sex on haemocytobiochemical profiling of silver tiger fish (Datnioides polota Hamilton, 1822). Comp. Clin. Patholo. 27(5):1335-1342.
Al-Aboudi, H. J. , A. J. Al-Rudainy and A. Abid Maktof. 2022. Accumulation of lead and cadmium in tissues of Cyprinus carpio collected from cages of Al-Gharraf River / Thi Qar / Iraq. Iraqi Journal of Agricultural Sciences, 53(4):819-824. https://doi.org/10.36103/ijas.v53i4.1594
Al-Rudainy, A.J. , S. A. Mustafa, A. A. Ashor and M. D. Bader. 2023. Role of kaolin on hematological, biochemical and survival rate of Cyprinus carpio challenged with Pesuydomonas aeruginosa. Iraqi Journal of Agricultural Sciences, 54(2): 472- 477. https://doi.org/10.36103/ijas.v54i2.1723
Al-Tamimi, M.S. and A. J. Al-Rudainy.2024. Effect of kaolin on hematological and biochemical parameters of Cyprinus carpio L. against copper sulfate toxicity. Iraqi Journal of Agricultural Sciences, 55(Special Issue):217-224. https://doi.org/10.36103/ijas.v55iSpecial.1900
Caponi, N., G.C. Collazzo, S.L. Jahn, G.L. Dotto, M.A. Mazutti and E.L. Foletto. 2017. Use of brazilian kaolin as a potential low-cost adsorbent for the removal of malachite green from colored effluents. Mat. Res. 20:14-22.
Carretero, M. I. and M. Pozo. 2010. Clay and non-clay minerals in the pharmaceutical and cosmetic industries Part II. Active Ingredients. Appl. Clay Sci. 47(3-4): 171-181.
Duffin, C. J., R.T.J. Moody and C.Gardner-Thorpe. 2013. A History of Geology and Medicine. Geolo. Soc. London. Special Publ. 375:1-6.
Duma, B.T. and H.G. Biggs. 1972. Standard Methods of Clinical Chemistry. Education of Academic Press. New York. 13:215-220.
Elwafai, N. A., F. I. El-Zamik, S. A. M. Mahgoub and A. Atia. 2020. Isolation of Aeromonas bacteriophage Avf07 from fish and its application for biological control of multidrug resistant local Aeromonas veronii afs2. Zagazig J. Agric. Res. 47(1): 179-197.
Faik , H.H. and S.A.Mustafa. 2023. Role of vitamin C and E on genotoxicity , hematological and biochemical investigation in Cyprinus carpio following zinc oxide nanoparticles exposure. Iraqi Journal of Agricultural Sciences. 54(3): 716-723. https://doi.org/10.36103/ijas.v54i3.1752
FAO. Food and Agriculture Organization (2016) .The State of World Fisheries and Aquaculture, Contributing to Food Security and Nutrition for All, Food and Agriculture Organization of the United Nations, Rome, Italy. http://www.fao.org/3/a-i5555e.pdf
Fernández-Bravo, A. and M.J.Figureueras. 2020. An update on the genus Aeromonas: Taxonomy, epidemiology, and pathogenicity. Micro-Organisms. 8(1): 129.
Harikrishnan, R., M.N.Rani and C.Balasundaram.2003. Hematological and biochemical parameters in common carp, Cyprinus carpio L. following herbal treatment for Aeromonas hydrophila infection. Aquaculture. 221(1-4): 41-50.
Hrubec, T. C., J. L. Cardinale and S. A. Smith. 2016.Hematology and plasma chemistry reference intervals for cultured tilapia (Oreochromis hybrid). Vet. Clin. Patholo. 29 (1):7-12.
Jha, A.K., A.K. Pal, S. Sahu and S.C. Mukherjee. 2007. Haemato-immunological responses to dietary yeast RNA, w-3fatty acid and b-carotene in Catla catla juveniles. Fish and Shellfish Immunol. 23: 917–927.
Kumar, S., R.P. Raman, P.K. Pandey, S. Mohanty, A. Kumar and K. Kumar. 2013. Effect of orally administered azadirachtin on non-specific immune parameters of goldfish Carassius auratus (Linn., 1758) and resistance against Aeromonas hydrophila. Fish and Shellfish Immunol. 34(2): 564-573.
Lapirova, T.B. and E.A.Flerova. 2013. Sravnitel’nyj Analiz Nekotoryh Immunofiziologicheskih Parametrov Krovi Shchuki Esox Iucius (L.) sudaka Stizostedion Lucioperca (L.). Vestnik Astrahanskogo Gosudarstvennogo Tekhnicheskogo Universiteta. Seriya: Rybnoe Hozyajstvo. 1:140-145.
Muslem, E. T., S.A.Sabeeh and W.A.A. Jheel. 2021. Detection of aerolysin and hemolysin genes in Aeromonas hydrophila isolates from in Al-Diwaniyah City, Iraq. Iranian J. Ichthyolo. 8: 392-396.
Mussab, K.A. and S.A.Mustafa. 2023. Efficiency of silver zeolite on hematology and survival rate in Cyprinus carpio infected with Saprolegnia Spp.. Iraqi Journal of Agricultural Sciences.55(Special Issue):225-232. https://doi.org/10.36103/ijas.v55iSpecial.1901
Mustafa , S. and A. J. Al-Rudainy. 2021. Impact of mercury chloride exposure on some of immunological and biochemical assays of common carp, Cyprinus carpio. Iraqi Journal of Agricultural Sciences. 52(3):547-551. https://doi.org/10.36103/ijas.v52i3.1341
Muthappa, N. A., S.Gupta, S.Yengkokpam, D.Debnath, N. Kumar, A.K.Pal and S.B.Jadhao. 2014. Lipotropes promote immunobiochemical plasticity and protect fish against low-dose pesticide-induced oxidative stress. Cell Stress and Chaperones. 19(1): 61-81.
Osman, H. A. M., N.G. Fadel and A.T.Ali. 2009. Biochemical and histopathological alterations in catfish, Clarias gariepinus infected with trypanosomiasis with special reference to immunization. Egyptain J. Comp. Patholo. Clin. Patholo. 22(3):164-181.
Patriche, T., N. Patriche, E. Bocioc and M.T. Coada. 2011. Serum biochemical parameter of farmed carp (C.carpio).Int. J. Bioflux Soc. 4(2):131-140.
Piotrowska, M. and M. Popowska. 2014. The prevalence of antibiotic resistance genes among Aeromonas species in aquatic environments. Ann. Microbiolo. 64(3): 921-934.
SAS 2012. Statistical Analysis System, User's Guide Statistical. Version 9.1th ed. SAS. Inst. Inc. Cary, N.C. USA.
Seth, N. and K.K.Saxena. 2003. Hematological responses in a freshwater fish channa punctatus due to fenvalerate. Bull. Environ. Cont. Toxicolo. 71(6): 1192-1199.
Siwicki, A. K., D.P. Anderson and J. Antychowicz. 1993. Nonspecific defence mechanisms assay in fish I; Phagocytic index, adherence and phagocytic ability of neutrophils (NBT test) and myeloperoxidase activity test fish disease diagnosis and preventions method. Wydawnictwo Instytutu Rybactwa Strodladowego. 95-104.
Suhail,R.N., S. A. Mustafa and E. E. Al-Oboddi.2022. Efficiency of silver nanoparticles as antibacterial against Aeromonas hydrophila isolated from infected common carp. Iraqi Journal of Agricultural Sciences. 53(3):589-597. https://doi.org/10.36103/ijas.v53i3.1568
Thrall, M. A., D.C.Bker, T.W.Camobell, D.Dinicola,M.J.Fettman,E.D.Lassen,A.Reber and G.Weiser . 2006. Veterinary Hematology and Clinical Chemistary. Lippincott Williams, Philadelphia. 214-219.
Witeska, M., K. Lugowska and E. Kondera. 2016. Reference values of hematological parameters for juvenile (Cyrpinus carpio). Bull. European Assoc. Fish Patholo. 36 (4): 169-180.
Yildirim‐Aksoy, M., H. Mohammed, E. Peatman, S.A. Fuller and B.H.Beck. 2018. Influence of kaolin clay on Aeromonas hydrophila growth, chemotaxis, and virulence to channel catfish. North Am. J. Aquac. 80(4): 427-435.
Downloads
Published
Issue
Section
License
Copyright (c) 2024 IRAQI JOURNAL OF AGRICULTURAL SCIENCES
![Creative Commons License](http://i.creativecommons.org/l/by-nc-nd/4.0/88x31.png)
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.