ESTIMATION OF THE WHOLE FLAVONOID, ANTIOXIDANT, ANTI BACTERIAL CHALLENGE CONCERNING VIOLA ODORATA (BANAFSHA) METHANOLIC EXTRACT.

Abstract

The objective concerning this study was to decide aggregation flavonoid content (TFC) primarily based of flavonoid-aluminum chloride (AlCl₃) and antioxidant activity by spectrophotometric method and determination of against bacterial action in Viola odorata (natural plant) utilizing reaction surface philosophy. Homegrown prescription or restorative plants have prompted the disclosure of various new medications, then non-tranquilize substances. Among it flora are kinds of the range group Viola has a region including family violaceae then accommodates round 19 species of comparable sort. Viola odorata (Banafsha) has considerable cell antioxidant properties. The among vitro records evidently portrayed the most cancers prevention agent adequacy of methanolic extricate, which was tried. spectrophotometrically strategy is a vital procedure to decide add up to total flavonoids, and cancer prevention agent action (reductive capacity and DPPH radical foragers action). Results showed that Viola odorata has high flavonoid substance which was (341± 0.280 µg/ml) in addition to tremendous antioxidant in a concentration dependant manner (ranged from 0.170 to 0.498 for 0.02 and 0.64 mg/ml respectively for reductive ability and 70.60 to 87.03 for 0.125 and 0.500 mg/ml in DPPD radical scavenging activity) and antibacterial in opposition to (Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli or Streptococcus).