GROWTH PERFORMANCE AND MICROBIAL POPULATION OF BROILER CHICKEN FED DIFFERENT LEVELS OF GRAPE POMACE MEAL
DOI:
https://doi.org/10.36103/5z6xhd24Keywords:
Feed alternatives, Intestinal health, Poultry dietAbstract
This study evaluated the effect of adding different levels of grape pomace meal on growth performance and microbial population of broilers dietary. A total of 275 one-day-old ROSS 308 chicks were randomly allotted into five treatments. Each treatment had five replicates, with 11 birds per replicate. The control birds were fed a diet consisting primarily of corn and soybean meal. The four treatments were fed a similar diet with the corn meal partially substituted by 2, 4, 6, and 8% grape pomace meal. Results showed no significant effect of grape pomace meal was observed on body weight and weight gain at days 24 and 35. The birds fed an 8% grape pomace meal diet reported higher (P<0.01) feed intake compared with other treatments. The feed conversion ratio increased (P<0.05) by 6% and 8% grape pomace meal diets. The population of E. coli and total bacteria declined (P<0.01), whereas the number of Lactobacillus spp inclined (P<0.01) with increasing the amount of grape pomace meal in the diets. In conclusion, the study indicated that feeding grape pomace meal up to 6% showed no deleterious effects on the growth performance, and the number of Lactobacillus spp was increased.
Received: 16/11/2024
Accepted: 9/2/2025
Published: 30/9/2026
References
Alhotan, R. A., 2021. Commercial poultry feed formulation: Status, challenges, and future expectations. World's Poultry Science Journal, 77(2):279–299. https://doi.org/10.1080/00439339.2021.1885849
Bennato, F., A. Di Luca, C. Martino, A. Ianni, E. Marone, L. Grotta, S. Ramazzotti, A. Cichelli, and G. Martino, 2020. Influence of grape pomace intake on nutritional value, lipid oxidation, and volatile profile of poultry meat. Foods, 9(4):508. https://doi.org/10.3390/foods9040508
Beres, C., G. N. S. Costa, I. Cabezudo, N. K. da Silva-James, A. S. C. Teles, A. P. G. Cruz, M. C. Mellinger-Silva, C. Tonon, L. M. C. Cabral, and S. P. Freitas, 2017. Towards integral utilization of grape pomace from winemaking process: A review. Waste Management, 68:581–594. https://doi.org/10.1016/j.wasman.2017.07.017
Borkar, V. D., A. B. Motghare, S. S. Bawaskar, and B. R. Wankhade, 2021. Studies on feeding of azolla meal on growth performance of Kadaknath poultry. International Journal of Fauna and Biological Studies, 8(1):105–108. https://doi.org/10.22271/23940522.2021.v8.i1b.801
Chedea, V. S., L. M. Palade, D. E. Marin, R. S. Pelmus, M. Habeanu, M. A. Gras, G. C. Pistol, and I. Taranu, 2018. Intestinal absorption and antioxidant activity of grape polyphenols. Nutrients, 10(5):588. https://doi.org/10.3390/nu10050588
Chee, S. H., P. Iji, M. Choct, L. L. Mikkelsen, and A. Kocher, 2010. Characterisation and response of intestinal microflora and mucins to manno-oligosaccharide and antibiotic supplementation in broiler chickens. British Poultry Science, 51:368–380. https://doi.org/10.1080/00071668.2010.499431
Chikwanha, O. C., E. Raffrenato, V. Muchenje, H. T. Musarurwa, and C. Mapiye, 2018. Varietal differences in nutrient, amino acid and mineral composition and in vitro rumen digestibility of grape (Vitis vinifera) pomace from the Cape Winelands vineyards in South Africa and impact of preservation techniques. Industrial Crops and Products, 118:30–37. https://doi.org/10.1016/j.indcrop.2018.03.040
Correddu, F., M. F. Caratzu, M. F. Lunesu, S. Carta, G. Pulina, and A. Nudda, 2023. Grape, pomegranate, olive, and tomato by-products fed to dairy ruminants improve milk fatty acid profile without depressing milk production. Foods, 12(4):865. https://doi.org/10.3390/foods12040865
de Carvalho Tavares, I. M., M. B. M. de Castilhos, M. A. Mauro, A. M. Ramos, R. T. de Souza, S. Gómez-Alonso, E. Gomes, R. Da-Silva, I. Hermosín-Gutiérrez, and E. S. Lago-Vanzela, 2019. BRS Violeta (BRS Rúbea × IAC 1398-21) grape juice powder produced by foam mat drying. Part I: Effect of drying temperature on phenolic compounds and antioxidant activity. Food Chemistry, 298:124971. https://doi.org/10.1016/j.foodchem.2019.124971
De Oliveira, C. O., A. A. P. Roll, F. M. Medeiros Gonçalves, D. C. N. Lopes, and E. G. Xavier, 2021. Olive pomace for the feeding of commercial poultry: Effects on performance, meat and eggs quality, haematological parameters, microbiota and immunity. World's Poultry Science Journal, 77(2):363–376. https://doi.org/10.1080/00439339.2021.1894409
Duncan, D. B., 1955. Multiple range and multiple F tests. Biometrics, 11:1–42. https://doi.org/10.2307/3001478
Dwyer, K., F. Hosseinian, and M. Rod, 2014. The market potential of grape waste alternatives. Journal of Food Research, 3(2):91–106. https://doi.org/10.5539/jfr.v3n2p91
Galanakis, C. M., 2012. Recovery of high added-value components from food waste: Conventional, emerging technologies and commercialized applications. Trends in Food Science and Technology, 26:68–87. https://doi.org/10.1016/j.tifs.2012.03.002
Goñi, I., A. Brenes, C. Centeno, A. Viveros, F. Saura-Calixto, A. Rebolé, I. Arija, and R. Estevez, 2007. Effect of dietary grape pomace and vitamin E on growth performance, nutrient digestibility, and susceptibility to meat lipid oxidation in chickens. Poultry Science, 86:508–516. https://doi.org/10.3382/ps.2006-00227
Gowman, A. C., M. C. Picard, A. Rodriguez-Uribe, M. Misra, H. Khalil, M. Thimmanagari, and A. K. Mohanty, 2019. Physicochemical analysis of apple and grape pomaces. BioResources, 14(2):3210–3230. https://doi.org/10.15376/biores.14.2.3210-3230
Gungor, E., A. Altop, and G. Erener, 2021. Effect of raw and fermented grape pomace on the growth performance, antioxidant status, intestinal morphology, and selected bacterial species in broiler chicks. Animals, 11(2):364. https://doi.org/10.3390/ani11020364.
Hernández-González, J.C., A. Martínez-Tapia, G. Lazcano-Hernández, B.E. García-Pérez, and N.S. Castrejón-Jiménez, 2021. Bacteriocins from lactic acid bacteria. a powerful alternative as antimicrobials, probiotics, and immunomodulators in veterinary medicine. Animals, 11(979), pp.1-17. https://doi.org/10.3390/ani11040979.
Kalli, E., I. Lappa, P. Bouchagier, P.A. Tarantilis, and E. Skotti, 2018. Novel application and industrial exploitation of winery by-products. Bioresources and Bioprocessing, 5(1), pp.1-21. https://doi.org/10.1186/s40643-018-0212-2.
Koshchaev, I., K. Mezinova, A. Ryadinskaya, O. Tatyanicheva, and N. Ordina.2020. Various sources of methionine in broiler chicken rations. E3S Web of Conferences, 210, 06009. https://doi.org/10.1051/e3sconf/202021006009h.
Kumanda, C., V. Mlambo, and C.M. Mnisi, 2019. From landfills to the dinner table: Red grape pomace waste as a nutraceutical for broiler chickens. Sustainability, 11(7), p.1931. https://doi.org/10.3390/su11071931.
Lagha, A.B., B. Haas, M. Gottschalk, and D. Grenier, 2017. Antimicrobial potential of bacteriocins in poultry and swine production. Veterinary Research, 48(1), pp.1-12. https://doi.org/10.1186/s13567-017-0451-0.
Matoo, F.A., G.A. Beat, M.T. Banday, and T.A.S. Ganaie, 2001. Performance of broilers fed on apple pomace diets supplemented with enzyme(s). Indian Journal of Animal Nutrition, 18(4), 349-352.
Moncalvo, A., L. Marinoni, R. Dordoni, G. Duserm Garrido, V. Lavelli, and G. Spigno, 2016. Waste grape skins: Evaluation of safety aspects to produce functional powders and extracts for the food sector. Food Additives and Contaminants: Part A, 33(7), pp.1116-1126. https://doi.org/10.1080/19440049.2016.1188582.
Muhlack, R.A., R. Potumarthi, and D.W. Jeffery, 2018. Sustainable wineries through waste valorisation: A review of grape marc utilisation for value-added products. Waste Management, 72, pp.99-118. https://doi.org/10.1016/j.wasman.2017.10.020.
NRC (National Research Council), 1994. Nutrient requirements of poultry. National Academies Press. https://doi.org/10.1093/japr/3.1.101.
Oliveira, M., F. Rodrigues, and B. Sarmento, 2021. Grape pomace: A comprehensive review on potential application as a natural source of bioactive compounds in skin health. Nutrients, 13(8), 2552. https://doi.org/10.3390/nu13082552.
Patterson, J.A., and K.M. Burkholder, 2003. Application of prebiotics and probiotics in poultry production. Poultry Science,82(4),627-631. https://doi.org/10.1093/ps/82.4.627.
Peixoto, C.M., M.I. Dias, M.J. Alves, R.C. Calhelha, L. Barros, S.P. Pinho, and I.C. Ferreira, 2018. Grape pomace as a source of phenolic compounds and diverse bioactive properties. Food Chemistry, 253, pp.132-138. https://doi.org/10.1016/j.foodchem.2018.01.062
Royce, L.A., P. Liu, M.J. Stebbins, B.C. Hanson, and L.R. Jarboe, 2013. The damaging effects of short chain fatty acids on Escherichia coli membranes. Applied Microbiology and Biotechnology, 97(18), pp.8317-8327. https://doi.org/10.1007/s00253-013-4923-1.
Sánchez-Pérez, S., O. Comas-Basté, J. Rabell-González, M.T. Veciana-Nogués, M.L. Latorre-Moratalla, and M.C. Vidal-Carou, 2018. Biogenic amines in plant-origin foods: Are they frequently underestimated in low-histamine diets? Foods, 7(12), p.205. https://doi.org/10.3390/foods7120205.
SAS, 2012. Statistical Analysis System, User’s Guide: Statistical. Version 9. SAS Institute Inc., Cary, NC, USA.
Sies, H., and D.P. Jones, 2020. Reactive oxygen species (ROS) as pleiotropic physiological signalling agents. Nature Reviews Molecular Cell Biology, 21(7), pp.363-383. https://doi.org/10.1038/s41580-020-0222-2.
Song, J., K. Xiao, Y.L. Ke, L.F. Jiao, C.H. Hu, Q.Y. Diao, B. Shi, and X.T. Zou, 2014. Effect of a probiotic mixture on intestinal microflora, morphology, and barrier integrity of broilers subjected to heat stress. Poultry Science, 93, 581-588. https://doi.org/10.3382/ps.2013-03671.
Taşeri, L., M. Aktaş, S. Şevik, M. Gülcü, G.U. Seçkin, and B. Aktekeli, 2018. Determination of drying kinetics and quality parameters of grape pomace dried with a heat pump dryer. Food Chemistry, 260, pp.152–159. https://doi.org/10.1016/j.foodchem.2018.03.122.
Thirumalaisamy, G., J. Muralidharan, S. Senthilkumar, R. Hema Sayee, and M. Priyadharsini, 2016. Cost-effective feeding of poultry. International Journal of Science, Environment and Technology, 5(6), pp. 3997-4005. https://doi.org/10.13140/RG.2.1.1276.0882.
Van den Abbeele, P., J. Ghyselinck, M. Marzorati, A. Villar, A. Zangara, C.R. Smidt, and E. Risco, 2020. In vitro evaluation of prebiotic properties of a commercial artichoke inflorescence extract revealed bifidogenic effects. Nutrients, 12(6):1552.https://doi.org/10.3390/nu12061552.
Vilela, A., I. Cruz, I. Oliveira, A. Pinto, and T. Pinto, 2022. Sensory and nutraceutical properties of infusions prepared with grape pomace and edible-coated dried—minced grapes. Coatings, 12:443. https://doi.org/10.3390/coatings12040443.
Wang, C., F. Zhao, Z. Li, X. Jin, X. Chen, Z. Geng, ... and C. Zhang, 2021. Effects of resveratrol on growth performance, intestinal development, and antioxidant status of broilers under heat stress. Animals, 11(5), p.1427. https://doi.org/10.3390/ani11051427.
Yang, J., X. Hou, P.S. Mir, and T.A. McAllister, 2010. Anti-Escherichia coli O157:H7 activity of free fatty acids under varying pH. Canadian Journal of Microbiology, 56(3):263-267. https://doi.org/10.1139/W09-136.
Yu, J., and M. Ahmedna, 2013. Functional components of grape pomace: Their composition, biological properties and potential applications. International Journal of Food Science and Technology, 48(2):221-237. https://doi.org/10.1111/j.1365-2621.2012.03081.x.
Zeaiter, Z., M.E. Regonesi, S. Cavini, M. Labra, G. Sello, and Di P.Gennaro, 2019. Extraction and characterization of inulin-type fructans from artichoke wastes and their effect on the growth of intestinal bacteria associated with health. BioMed Research International, 2019,:1-12. https://doi.org/10.1155/2019/9785746.
Zhang, C., K.K. Chen, X.H. Zhao, and Z.Y. Geng, 2018. Protective effects of resveratrol against high ambient temperature-induced spleen dysplasia in broilers through modulating splenic redox status and apoptosis. Journal of the Science of Food and Agriculture, 98,:5409–5417. https://doi.org/10.1002/jsfa.8892.
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