HEMATOLOGICAL AND MOLECULAR DETECTION OF THEILERIA ANNULATA FROM THI-QAR GOVERNORATE AND THE MARSHES CATTLE ISOLATE

Authors

  • Mohanad Abbas Talab College of Veterinary Medicine, University of Baghdad
  • Inam Badr Falih College of Veterinary Medicine, University of Baghdad

DOI:

https://doi.org/10.36103/xrkja676

Keywords:

marshes, pcr, tropical theileriosis, cox-1 gene, cbc

Abstract

This study aims to investigate Theileria annulata infection in cattle herds in Thi-qar governorate and the Marshes. The 115 blood samples were collected from cattle of different ages and sexes in the Dhi-qar governorate and the marshes in Iraq. Microscopic examination showed that the prevalence of Theileria in cattle was 43.4%, based on the microscopic examination of blood samples stained with Giemsa stain. Analysis of some blood parameters: RBCs, HGBPCV, MCV, MCH, MCHC, RDW-CV, RDW-SD, PLT, WBCs, Lymph, and Granulocytes revealed significant differentiation due to Theileria annulata infection.  Conventional PCR analysis of blood samples targeting the tams-1 and cox-1 genes revealed 69.56% positivity for T. annulata. The cox-1 gene (1200 bp) and tams-1 gene (441 bp) sequences were deposited in GenBank under accession numbers (LC852773.1, LC852774.1, LC852775.1) and (LC852776.1, LC852777.1, LC852778.1), respectively. Phylogenetic analysis showed that the tams-1 gene of the isolates shared high homology and evolutionary relationships with strains from the Netherlands, Scotland, India, China, South Africa, and Japan, indicating genetic conservation across regions.

Received: 16/1/2025

Accepted: 30/4/2025

Published: 31/8/2026

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Published

2026-08-31

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Vol. 57 No. 8 (2026)

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Copyright (c) 2026 Mohanad Abbas Talab , Inam Badr Falih

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How to Cite

Abbas Talab , M., & Badr Falih , I. (2026). HEMATOLOGICAL AND MOLECULAR DETECTION OF THEILERIA ANNULATA FROM THI-QAR GOVERNORATE AND THE MARSHES CATTLE ISOLATE . IRAQI JOURNAL OF AGRICULTURAL SCIENCES, 57(8), 2148-2162. https://doi.org/10.36103/xrkja676