ENHANCING THE ACCUMULATION OF CERTAIN ACTIVE COMPOUNDS IN ALOE VERA GEL UNDER THE EFFECT OF VERMICOMPOST AND FOLIAR SPRAY WITH SPIRULINA ALGAE EXTRACT
DOI:
https://doi.org/10.36103/08nd6t74Keywords:
aloe plant, biofertilizer ,, organic fertilizer, secondary compoundsAbstract
The experiment was conducted at the Agricultural Research Station A, College of Agricultural Engineering Sciences, University of Baghdad – Al-Jadriya, on aloe vera plants during the spring season of 2023, starting from April 19, 2023, to October 19, 2023. The objective was to investigate the individual and interactive effects of soil application of vermicompost and foliar spraying with Spirulina algae extract on the content of certain elements, carbohydrates, protein, and some bioactive compounds within the Aloe plant. The experiment was implemented according to a Randomized Complete Block Design (RCBD) with a Split-plot arrangement and three replicates. The results indicated the superiority of the two-way interaction treatment involving the addition of 15 tons ha⁻¹ of vermicompost to the soil in conjunction with foliar spraying of Spirulina extract at a concentration of 8 g L⁻¹ in the content of the dried leaves for each of the following: nitrogen percentage (0.463 %), phosphorus (0.133 %), potassium (2.79 %), calcium (215.7 ppm), total carbohydrates (5.65 %), protein (2.89 %), and both aloin compound (271 mg plant⁻¹) and aloinmodin compound (172.57 mg plant⁻¹), as well as phenylalanin and cinnamic acids (39.73 mg 100 g⁻¹ and 54.47 parts per million, respectively), total phenols (136.90 mg 100 g⁻¹ of dried gel), and total flavonoids (106.5 mg 100 g⁻¹ of dried gel).
Received: 27/4/2025
Accepted: 9/7/2025
Published: 31/7/2026
References
Al Dayel, M. F., and F. El Sherif. 2022. Spirulina platensis foliar spraying Curcuma longa has improved growth, yield, and curcuminoid biosynthesis gene expression, as well as curcuminoid accumulation. Horticulturae; 8 (6): 469. https://doi.org/10.3390/horticulturae8060469 DOI: https://doi.org/10.3390/horticulturae8060469
Al-Naimi, J. H.. 2010. Treatment with Fruit Trees, Shrubs, and Forests. Baghdad, Iraq: Al-Hourra Publishing House.
Al-Rawi,M.K, and A. A.M. Khalaf Allah. 2000. Design and Analysis of Agricultural Experiments. 2nd ed. Mosul, Iraq: Dar Al-Kutub and Publishing, University of Mosul.
Al-Ubadi, F. A. H., and N. A. Al-Zubai. 2017. Applications in Statistics and Analysis of Agricultural Experiments. University of Baghdad, College of Agriculture, p. 200.
Anastasia, E. G.; I. F. Illias; J. J. D. Maksimovic; V. M. Maksi and B. D. Zivanovic. 2012. The effects of plant growth regulators on growth, yield and phenolic profile of lentil plants. Journal of food composition and analysis 28: 46–53. https://doi.org/10.1016/j.jfca.2012.05.002. DOI: https://doi.org/10.1016/j.jfca.2012.06.005
Anitha, L.; G. S. Bramari, and P. Kalpana. 2016. Effect of supplementation of Spirulina platensis to enhance the zinc status in plants of Amaranthus gangeticus, Phaseolus aureus and tomato. Advances in bioscience and biotechnology 7 (6): 289–299. https://doi.org/10.4236/abb.2016.76027. DOI: https://doi.org/10.4236/abb.2016.76027
Association of Official Analytical Chemists(AOAC). 1980. Official Methods of Analysis. 13th ed. AOAC International.
Chiang, S.; K. B. Swamy;T. W. Hsu; Z. T. Tsai;H. H. Lu; D. Wang and H. K. Tsai. 2012. Analysis of the association between transcription factor binding site variants and distinct accompanying regulatory motifs in yeast. Gene 491 (2): 237–245. https://doi.org/10.1016/j.gene.2011.09.034. DOI: https://doi.org/10.1016/j.gene.2011.08.028
DataApex. 2015. Clarity Chromatography Software (Version 5.0) [Computer software]. Prague, Czech Republic: DataApex Ltd.
El Sherif, F.; N. Albotnoor; Y. K. Yap; A. Meligy, and S. Khattab. 2020. Enhanced bioactive compounds composition in Lavandula officinalis in-vitro plantlets using NaCl and Moringa oleifera, Aloe vera and Spirulina platensis extracts. Industrial crops and products 157: 112890. https://doi.org/10.1016/j.indcrop.2020.112890. DOI: https://doi.org/10.1016/j.indcrop.2020.112890
Gholami, H.; M. J. Saharkhiz;F. R. Fard;A. Ghani, and F. Nadaf. 2018. Humic acid and vermicompost increased bioactive components, antioxidant activity and herb yield of chicory (Cichorium intybus L.). Biocatalysis and agricultural biotechnology 14: 286–292. https://doi.org/10.1016/j.bcab.2018.07.004 DOI: https://doi.org/10.1016/j.bcab.2018.03.021
Godlewska, K.; I. Michalak; P. Pacyga; S. Baśladyńska and K. Chojnacka. 2019. Potential applications of cyanobacteria: Spirulina platensis filtrates and homogenates in agriculture. World journal of microbiology and biotechnology 35: 1–18. https://doi.org/10.1007/s11274-019-2653-6. DOI: https://doi.org/10.1007/s11274-019-2653-6
Habibatni, S.; A. F. Zohra; H. Khalida;S. Anwa; I. Mamsi and N. A. A. Ali. 2017. In vitro antioxidant, xanthine oxidase-inhibitory and in vivo anti-inflammatory, analgesic, antipyretic activity of Onopordum acanthium. International journal of phytomedicine 9 (1): 92–100. https://doi.org/10.5138/09750185.2030. DOI: https://doi.org/10.5138/09750185.2030
Hussain, N.; T. Abbasi, and S. A. Abbasi. 2020. Evaluating the fertilizer and pesticidal value of vermicompost generated from a toxic and allelopathic weed Ipomoea. Journal of the Saudi Society of Agricultural Sciences 19: 43–50. https://doi.org/10.1016/j.jssas.2018.05.005. DOI: https://doi.org/10.1016/j.jssas.2018.05.005
Joslyn, M. A. 1970. Methods in Food Analysis. 2nd ed. Academic Press.
Kim, D. M.;J. Y. Jung;H. K. Lee; Y. S. Kwon and Y. M. Choi. 2021. Determination and profiling of secondary metabolites in Aloe vera, Aloe arborescens, and Aloe saponaria. Biomedical journal of scientific & technical research 40: 32555–32563. https://doi.org/10.26717/BJSTR. DOI: https://doi.org/10.26717/BJSTR.2021.40.006499
Laouini, S. E. and Mohammed R. Q. 2017. Phytochemical screening, in vitro antioxidant and antibacterial activity of Rumex vesicarius L. extract. Scientific study & research: Chemistry & chemical engineering, biotechnology, food industry 18 (4): 367–376.
Logaranjan, K.; T. Devasena, and P. Kannaiyan. 2013. Quantitative detection of aloin and related compounds present in herbal products and Aloe vera plant extract using HPLC method. American journal of analytical chemistry 4 (10): 600–605. https://doi.org/10.4236/ajac.2013.410071. DOI: https://doi.org/10.4236/ajac.2013.410071
López-Padrón, I.; L. Martínez-González;G. Pérez-Domínguez; Y. Reyes-Guerrero; M. Núñez-Vázquez, and J. Cabrera-Rodríguez. 2020. Algae and their uses in agriculture: An update. Cultivos tropicales 41 (10). https://doi.org/10.1016/j.culttropica.2020.10.005.
Mahmud, M.;S. Ramasamy; R. Othman;R. Abdullah and J. Syafawati Yaacob. 2019. Effect of vermicompost application on bioactive properties and antioxidant potential of MD2 pineapple fruits. Agronomy 9 (2): 97. https://doi.org/10.3390/agronomy9020097. DOI: https://doi.org/10.3390/agronomy9020097
Nematian, A.;M. Vanaei; J. Tomovska; M. A. Shariati, and M. Kaviani. 2012. Effects of plant density and vermicompost on the rate of two active substances, aloenin and aloe emodin of Aloe vera (Aloe barbadensis Mill.). International journal of pharmaceutical research & allied sciences 4 (2): 132–137.
Prabha, M. L.; I. A. Jayraaj; R. Jayraaj and D. S. Rao. 2007. Effect of vermicompost on growth parameters of selected vegetable and medicinal plants. Asian journal of microbiology 9: 321–326.
Priyadarshani, A. M. B. and N. Rath. 2012. Production of vitamin B12 by microorganisms: A review. International journal of bio-technology and research 2 (1): 1–14.
Rasmus, D.L.; V. Hecke; J., Jørgensen; H. Bukh;I. Christian; et al. 2018. High-throughput analysis of amino acids in plant materials by single quadrupole mass spectrometry. Plant Methods, 14(1), Article 4. https://doi.org/10.1186/s13007-018-0277-8. DOI: https://doi.org/10.1186/s13007-018-0277-8
Roy, S.; K. Arunachalam;D. B. Kumar and A. Arunachalam. 2010. Effect of organic amendments of soil on growth and productivity of three common crops viz. Zea mays, Phaseolus vulgaris and Abelmoschus esculentus. Applied soil ecology 45: 78–84. https://doi.org/10.1016/j.apsoil.2010.02.004. DOI: https://doi.org/10.1016/j.apsoil.2010.02.004
Salman, S. A.and Z. M. Abdul-Qader. 2022. Effect of harvest dates, mycorrhiza, and some biostimulants on some characteristics of vegetative and root growth and some components of volatile oil from the active compounds in the sweetscented geranium plant (Pelargonium graveolens L. Herit). Iraqi journal of agricultural research 26 (1): 35–55. http://www.ijarmoa.gov.iq. DOI: https://doi.org/10.65814/ijar.2022.26.1.35
Scriver, C. R.; A. L. Beaudet;W. S. Sly, and D. Valle, eds. 2001. The Metabolic And Molecular Bases of Inherited Disease. 8th ed., Vols. 1–4. New York: McGraw-Hill.
Sreenivasan, E. 2018. Handbook of Vermicomposting Technology. The Western India Plywoods Ltd.
Yanni, Y. G.;A. A. Elashmouny and A. Y. Elsadany. 2020. Differential response of cotton growth, yield and fiber quality to foliar application of Spirulina platensis and urea fertilizer. Asian journal of advances in agricultural research 12 (1): 29–40. https://doi.org/10.9734/ajaar/2020/v12i130086. DOI: https://doi.org/10.9734/ajaar/2020/v12i130072
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