ROLE OF MELATONIN, BORON AND CHLORELLA VULGARIS ON THE ANTIOXIDANT ENZYMES OF DATE PALMS IN A DESERT ENVIRONMENT
DOI:
https://doi.org/10.36103/d9mvbw42Keywords:
biofertilizer, catalase, combating desertification, peroxidase, polyphenolAbstract
This study aimed to evaluate the effects of melatonin, boron, and Chlorella vulgaris algae on the antioxidant enzyme activity and nutrient status of date palm trees under environmental stress conditions. The study was conducted during the growing season in 2024 at Fadak Date Palm Station, located in the Karbala desert, on Phoenix dactylifera L. ' var. Sayer' trees. With A total of 54 uniforms were seven -year -old palms chosen for the experiment. Melatonin was applied to three concentrations (0, 50 and 100 mg L⁻1) as foliar spray, while boron (in the form of boric acid) were sprayed at two concentrations (0 and 500 mg L⁻1). In addition, Clorella vulgaris algae were incorporated in the soil in three concentrations (0, 100 and 200 ml L⁻1). The results demonstrated that melatonin and boron play an important and dynamic role in increasing the activity of antioxidant enzymes (peroxidase, catalase, and polyphenol oxidase) under drought-induced stress conditions, which improves the nutrition and physical condition of the palms and eventually reflected in fruit quality. In addition, the soil application of Chlorella vulgaris at varying levels increased sharply nitrogen and potassium content in palm leaves. These factors combined alleviate environmental pressures on date palms in desert conditions.
Received: 16/4/2025
Accepted: 13/7/2025
Published: 31/7/2026
References
Abinandan, S., Subashchandrabose, S. R., Venkateswarlu, K., & Megharaj, M. (2019). Soil microalgae and cyanobacteria: the biotechnological potential in the maintenance of soil fertility and health. Critical reviews in biotechnology, 39(8), 981-998. https://doi.org/10.1080/07388551.2019.1654972 DOI: https://doi.org/10.1080/07388551.2019.1654972
Ahmad, S., Wang, G. Y., Muhammad, I., Farooq, S., Kamran, M., Ahmad, I., ... & Zhou, X. B. (2022). Application of melatonin-mediated modulation of drought tolerance by regulating photosynthetic efficiency, chloroplast ultrastructure, and endogenous hormones in maize. Chemical and Biological Technologies in Agriculture, 9(1), 5.
https://doi.org/10.1186/s40538-021-00272-1 DOI: https://doi.org/10.1186/s40538-021-00272-1
Al-Khafaf, S., Al-Shiraqui, R. M. K., and Shabana, H. R. 1998. Proceedings of the First International Conference on Date Palms. Al-Ain, U.A.E,pp:247.
https://www.actahort.org/books/736/index.htm
Arredondo, G., & Bonomelli, C. (2023). Effect of three boron concentrations in soil on growth and physiology in sweet cherry trees. Plants, 12(6), 1240. https://doi.org/10.3390/plants12061240 DOI: https://doi.org/10.3390/plants12061240
Al-Asally, M. E., and S.H.Al-Hijemy.2023. Response of bio-stimulator and foliar spray of nano-nitrogen on growth characteristics of two mandarin seedlings. IOP Conference Series: Earth and Environmental Science 1158 (4): 042046.
DOI: 10.1088/1755-1315/1158/4/042046 DOI: https://doi.org/10.1088/1755-1315/1158/4/042046
Al-Hassany, H. A., & Abbood, M. R. (2021). Effect of Boron Spray and Pollination Methods on" Zahdi" Date Palm Yield and Fruits Quality. Annals of the Romanian Society for Cell Biology, 25(6), 10203-10208.
http://annalsofrscb.ro/index.php/journal/article/download/7381/5481/13166
Abdel-Karim, H.A., Nehad, M.A., El-Rouby, K.M. and Roshdy, K.A., 2015. Effect of foliar application of Boron and Zinc on fruit set, yield and some fruit characteristics of Fuerte avocado. Research Journal of Pharmaceutical, Biological and Chemical Sciences, Vol. 6, No. 5, 443-449 http://rjpbcs.com/pdf/2015_6(5)/[62].pdf
Altaf, M. A., Shahid, R., Ren, M. X., Naz, S., Altaf, M. M., Khan, L. U., ... & Ahmad, P. (2022). Melatonin improves drought stress tolerance of tomato by modulating plant growth, root architecture, photosynthesis, and antioxidant defense system. Antioxidants, 11(2), 309.
https://doi.org/10.3390/antiox11020309 DOI: https://doi.org/10.3390/antiox11020309
Alvarez, A. L., Weyers, S. L., Goemann, H. M., Peyton, B. M., & Gardner, R. D. (2021). Microalgae, soil and plants: A critical review of microalgae as renewable resources for agriculture. Algal Research, 54, 102200.
https://doi.org/10.1016/j.algal.2021.102200 DOI: https://doi.org/10.1016/j.algal.2021.102200
Arnao, M. B., & Hernández‐Ruiz, J. (2021). Melatonin as a regulatory hub of plant hormone levels and action in stress situations. Plant Biology, 23, 7-19.
https://doi.org/10.1111/plb.13202 DOI: https://doi.org/10.1111/plb.13202
Back, K. (2021). Melatonin metabolism, signaling and possible roles in plants. The Plant Journal, 105(2), 376-391.
https://doi.org/10.1111/tpj.14915 DOI: https://doi.org/10.1111/tpj.14915
Çakirsoy, I., Miyamoto, T., & Ohtake, N. (2022). Physiology of microalgae and their application to sustainable agriculture: A mini-review. Frontiers in Plant Science, 13, 1005991.
https://doi.org/10.3389/fpls.2022.1005991 DOI: https://doi.org/10.3389/fpls.2022.1005991
Chang, Q., Zhang, L., Chen, S., Gong, M., Liu, L., Hou, X., & Sun, Y. (2023). Exogenous melatonin enhances the yield and secondary metabolite contents of Prunella vulgaris by modulating antioxidant system, root architecture and photosynthetic capacity. Plants, 12(5), 1129.
https://doi.org/10.3390/plants12051129 DOI: https://doi.org/10.3390/plants12051129
Dineshkumar, R., Kumaravel, R., Gopalsamy, J., Sikder, M. N. A., & Sampathkumar, P. (2018). Microalgae as bio-fertilizers for rice growth and seed yield productivity. Waste and biomass valorization, 9(5), 793-800.
https://doi.org/10.1007/S12649-017-9873-5 DOI: https://doi.org/10.1007/s12649-017-9873-5
Ding, F., Liu, B., & Zhang, S. (2017). Exogenous melatonin ameliorates cold-induced damage in tomato plants. Scientia Horticulturae, 219, 264-271.
https://doi.org/10.1016/j.scienta.2017.03.029 DOI: https://doi.org/10.1016/j.scienta.2017.03.029
El-Kosary, S., Rashedy, A., El-Sharabasy, S., & Abd Allah, A. M. (2023). Applications of Calcium Boron or Potassium Phosphite Combined with Different Fruit Thinning Styles to Enhance Fruit Quality of Barhee date palm. Egyptian Journal of Chemistry, 66(3), 427-438.
https://doi.org/10.21608/EJCHEM.2022.141561.6195 DOI: https://doi.org/10.21608/ejchem.2022.141561.6195
Faisal, H. A., Abdullah, A. S. A., & Hzaa, A. Y. L. (2024). Response of Date Palm Trees (Phoenix dactylifera L.) to Treatment with Boron and Silica Sol Nutrient Solution under Saline Growing Conditions. Diyala Agricultural Sciences Journal, 16(2), 70-83.
doi: https://doi.org/10.52951/dasj.24160206 DOI: https://doi.org/10.52951/dasj.24160206
Goth, L. (1991). A simple method for determination of serum catalase activity and revision of reference range. Clinica chimica acta, 196(2-3), 143-151.
https://doi.org/10.1016/0009-8981(91)90067-m DOI: https://doi.org/10.1016/0009-8981(91)90067-M
Haynes, R. J. (1980). A comparison of two modified Kjeldahl digestion techniques for multi‐element plant analysis with conventional wet and dry ashing methods. Communications in Soil Science and Plant Analysis, 11(5), 459-467.
https://doi.org/10.1080/00103628009367053 DOI: https://doi.org/10.1080/00103628009367053
Hu, E., Liu, M., Zhou, R., Jiang, F., Sun, M., Wen, J., & Wu, Z. (2021). Relationship between melatonin and abscisic acid in response to salt stress of tomato. Scientia Horticulturae, 285, 110176.
https://doi:10.1016/j.scienta.2021.110176 DOI: https://doi.org/10.1016/j.scienta.2021.110176
Imran, M., Aaqil Khan, M., Shahzad, R., Bilal, S., Khan, M., Yun, B. W., ... & Lee, I. J. (2021). Melatonin ameliorates thermotolerance in soybean seedling through balancing redox homeostasis and modulating antioxidant defense, phytohormones and polyamines biosynthesis. Molecules, 26(17), 5116.
https://doi.org/10.3390/molecules26175116 DOI: https://doi.org/10.3390/molecules26175116
Ismail, I. A., Abo-El-Ez, A. T., Gad-Elkarim, M. R., & Hussien, M. A. (2022). Effect of different levels of irrigation and micronutrient fertilization on productivity of Seewy date palms. Journal of Sohag Agriscience (JSAS), 7(2), 20-31. (https://doi.org/10.21608/jsasj.2022.284246) DOI: https://doi.org/10.21608/jsasj.2022.284246
Jabbar, S. H., & Hassan, Z. A. (2020). Effect of spraying date of gibberellic acid and boron on some physical characteristics of palm trees CV. khadhrawi. Plant Archives (09725210),
20(1).
http://www.plantarchives.org/20-1/435-442%20(5750).pdf.
Kraiser, T., Gras, D. E., Gutiérrez, A. G., González, B., & Gutiérrez, R. A. (2011). A holistic view of nitrogen acquisition in plants. Journal of experimental botany, 62(4), 1455-1466.
https://doi.org/10.1093/jxb/erq425 DOI: https://doi.org/10.1093/jxb/erq425
Kumar, A., S.Mishra, V. Bahadur, and N.R. Ravindra. 2024. Effect of foliar spray of boron and zinc on flowering, fruit set and quality of olive (Olea europaea L.). International Journal of Advanced Biochemistry Research; 8(6): 129-135. DOI: https://doi.org/10.33545/26174693.2024.v8.i6b.1276
(https://doi.org/10.33545/26174693.2024.v8.i6b.1276)
Li, Y., Zhang, L., Yu, Y., Zeng, H., Deng, L., Zhu, L., & Wang, Y. (2022). Melatonin-induced resilience strategies against the damaging impacts of drought stress in rice. Agronomy, 12(4), 813.
https://doi.org/10.3390/agronomy12040813 DOI: https://doi.org/10.3390/agronomy12040813
Li, Z., Su, X., Chen, Y., Fan, X., He, L., Guo, J., & Yang, Q. (2021). Melatonin improves drought resistance in maize seedlings by enhancing the antioxidant system and regulating abscisic acid metabolism to maintain stomatal opening under PEG-induced drought. Journal of Plant Biology, 64(4), 299-312.
https://doi.org/10.1007/s12374-021-09297-3 DOI: https://doi.org/10.1007/s12374-021-09297-3
Liu, Y., Riaz, M., Yan, L., Zeng, Y., & Cuncang, J. (2019). Boron and calcium deficiency disturbing the growth of trifoliate rootstock seedlings (Poncirus trifoliate L.) by changing root architecture and cell wall. Plant Physiology and Biochemistry, 144, 345-354. DOI: https://doi.org/10.1016/j.plaphy.2019.10.007
(https://doi.org/10.1016/j.plaphy.2019.10.007)
Marri, C., Frazzoli, A., Hochkoeppler, A., & Poggi, V. (2003). Purification of a polyphenol oxidase isoform from potato (Solanum tuberosum) tubers. Phytochemistry, 63(7), 745-752. https://doi.org/10.1016/S0031-9422(03)00353-4 DOI: https://doi.org/10.1016/S0031-9422(03)00353-4
Mitter, E. K., Tosi, M., Obregón, D., Dunfield, K. E., & Germida, J. J. (2021). Rethinking crop nutrition in times of modern microbiology: innovative biofertilizer technologies. Frontiers in Sustainable Food Systems, 5, 606815.
https://doi.org/10.3389/fsufs.2021.606815 DOI: https://doi.org/10.3389/fsufs.2021.606815
Nosheen, S., Ajmal, I., & Song, Y. (2021). Microbes as biofertilizers, a potential approach for sustainable crop production. Sustainability, 13(4), 1868.
https://doi.org/10.3390/su13041868 DOI: https://doi.org/10.3390/su13041868
Pereira, G. L., Siqueira, J. A., Batista-Silva, W., Cardoso, F. B., Nunes-Nesi, A., & Araújo, W. L. (2021). Boron: more than an essential element for land plants?. Frontiers in Plant Science, 11, 610307.
https://doi.org/10.3389/fpls.2020.610307 DOI: https://doi.org/10.3389/fpls.2020.610307
Ödemiş, B., & Uncu, S. (2022). Determining effects of foliar boron applications on yield and fruit quality of apricot trees for reducing water stress. Mustafa Kemal Üniversitesi Tarım Bilimleri Dergisi, 27(1), 47-60. https://doi.org/10.37908/mkutbd.1009679 DOI: https://doi.org/10.37908/mkutbd.1009679
Sadak, A., & Şensoy, S. (2022). Utilization of microalgae [Chlorella vulgaris Beyerinck (Beijerinck)] on plant growth and nutrient uptake of garden cress (Lepidium sativum L.) grown in different fertilizer applications. International Journal of Agriculture Environment and Food Sciences, 6(2), 240-245.
https://doi.org/10.31015/jaefs.2022.2.6 DOI: https://doi.org/10.31015/jaefs.2022.2.6
Sadiq, S. M. (2023, November). Effect of foliar spraying with boron and brassinolide on the growth and flowering of gladiolus. In IOP Conference Series: Earth and Environmental Science (Vol. 1259, No. 1, p. 012060). IOP Publishing.
https://doi.org/10.1088/1755-1315/1259/1/012060 DOI: https://doi.org/10.1088/1755-1315/1259/1/012060
Sajid, M.,A. Basit, S.T. Shah, A. Khan, I. Ullah, M. Bilal, M.S. Khan, and W. Khan. 2024. Enhancing the quality and fruit yield of sweet cherry (Prunus avium) cultivars by foliar application of boron. Applied Fruit Science 66 (2): 485–494. DOI: 10.1007/s10341-023-01023-2 DOI: https://doi.org/10.1007/s10341-023-01023-2
Sayed, H.F. and A.M. Gomaa.2024. evaluating the impact of spraying nano potassium and nano boron on productivity and fruit quality of medjool Date Palm. Journal of Plant Production, 15(7):p.373-378. https://doi: 10.21608/jpp.2024.284306.1331 DOI: https://doi.org/10.21608/jpp.2024.284306.1331
Sharafi, Y. and Raina, M., 2021. Effect of Boron on Pollen Attributes in Different Cultivars of Malus domestica L. Y. Sharafi, M. Raina. National Academy Science Letters, 44(3), pp.189-194. https://doi.org/10.1007/s40009-020-00986-0 DOI: https://doi.org/10.1007/s40009-020-00986-0
Shireen, F., Nawaz, M. A., Chen, C., Zhang, Q., Zheng, Z., Sohail, H., ... & Bie, Z. (2018). Boron: functions and approaches to enhance its availability in plants for sustainable agriculture. International journal of molecular sciences, 19(7), 1856.
https://doi.org/10.3390/ijms19071856 DOI: https://doi.org/10.3390/ijms19071856
Stal, L. J. 2015. Nitrogen Fixation in Cyanobacteria. John Wiley & Sons.pp:31
https://doi.org/10.1002/9780470015902.a0021159.pub2 DOI: https://doi.org/10.1002/9780470015902.a0021159.pub2
Tiwari, R. K., Kumar, R., Lal, M. K., Kumar, A., Altaf, M. A., Devi, R., ... & Aftab, T. (2023). RETRACTED ARTICLE: Melatonin-Polyamine Interplay in the Regulation of Stress Responses in Plants. Journal of Plant Growth Regulation, 42(8), 4834-4850.https://doi.org/10.1007/s00344-022-10717-y DOI: https://doi.org/10.1007/s00344-022-10717-y
Trivedi, P., Delgado-Baquerizo, M., Anderson, I. C., & Singh, B. K. (2016). Response of soil properties and microbial communities to agriculture: Implications for primary productivity and soil health
indicators. Frontiers in plant science, 7, 990.
https://doi.org/10.3389/fpls.2016.00990 DOI: https://doi.org/10.3389/fpls.2016.00990
Uysal, O., Uysal, F. O., & Ekinci, K. (2015). Evaluation of microalgae as microbial fertilizer. European Journal of Sustainable Development, 4(2), 77-77.
https://doi.org/10.14207/ejsd.2015.v4n2p77 DOI: https://doi.org/10.14207/ejsd.2015.v4n2p77
Vera-Maldonado, P., Aquea, F., Reyes-Díaz, M., Cárcamo-Fincheira, P., Soto-Cerda, B., Nunes-Nesi, A., & Inostroza-Blancheteau, C. (2024). Role of boron and its interaction with other elements in plants. Frontiers in Plant Science, 15, 1332459.
https://doi.org/10.3389/fpls.2024.1332459 DOI: https://doi.org/10.3389/fpls.2024.1332459
Whitaker, J. R., and R.A. Bernhard. 1972. Experiment for an Introduction to Enzymology. The Wibber Press, USA. DOI:10.2307/1291957 DOI: https://doi.org/10.2307/1291957
Walli, S., Hafiz, I.A., Khan, R.I. et al. Zinc and Boron Application at Different Phenological Stages Alleviates Tree Growth, Fruit Yield and Quality of Sweet Orange Cv. ‘Blood Red’. Gesunde Pflanzen 74, 385–396 (2022). https://doi.org/10.1007/s10343-021-00616-9 DOI: https://doi.org/10.1007/s10343-021-00616-9
Zeng, W., S. Mostafa, Z. Lu, and B. Jin. 2022. Melatonin-mediated abiotic stress tolerance in plants. Frontiers in Plant Science 13: 847175.
https://doi.org/10.3389/fpls.2022.847175 DOI: https://doi.org/10.3389/fpls.2022.847175
Zhang, L., C.Sun, H. Tian, J. Xu, and X. Wu. 2024. Foliar spraying of boron prolongs preservation period of strawberry fruits by altering boron form and boron distribution in cell. Frontiers in Plant Science 15: 1457694.
https://doi.org/10.3389/fpls.2024.1457694 DOI: https://doi.org/10.3389/fpls.2024.1457694
Zittis, G., M. Almazroui, P. Alpert, P. Ciais, W. Cramer, Y. Dahdal, , ... & J. Lelieveld.2022. Climate change and weather extremes in the Eastern Mediterranean and Middle East. Reviews of geophysics, 60(3), e2021RG000762. DOI: https://doi.org/10.1029/2021RG000762
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