CLINICAL AND MOLECULAR STUDY OF UPPER RESPIRATORY TRACT INFECTIONS CAUSED BY KLEBSIELLA PNEUMONIAE IN CATS
DOI:
https://doi.org/10.36103/606thc10Keywords:
Feline, PCR, phylogenetic, respiratory infection,, IraqAbstract
The study was included clinical and molecular study of Klebsiella pneumoniae from upper respiratory tract of cats in 150 (with and without) respiratory signs of (60 local breed and 90 different breed) and both sexes (male70 and female 80) with different ages including more than one year ( 85) and less than one year (65) attended in veterinary clinics and Baghdad veterinary hospital in Baghdad city at the period from October 2023 to September 2024. The 150 nasal swabs were cultured on macConkey and Klebsiella chrom agar, then the bacteria were examined by Gram stain with different biochemical and antimicrobial study using Vitek-2 analysis. In addition, the molecular detection of 16S r RNA gene of K. pneumoniae. The results of clinical study showed increasing with signs of fever (76.2%) and sneezing (61.9%) for K. pneumoniae. The total isolation rate was 21 (14%). The DNA of bacteria was extracted and the positive products (21) of K. pneumoniae was successfully amplified by using specific primers at 260 bp of 16S r RNA gene of K. pneumoniae. The isolates of K. pneumoniae were highly resistant to Ampicillin (71.4%) and Piperacillin (66.6%) while were sensitive (100%) to Ceftolozan. The infection rate of bacteria was significantly increased at age group less than one year and females. A significant increase was observed in Himalaya than other breeds. The highest percentage of infections by K. pneumonia appeared in December (76.9%) . The results of sequencing of the (5) isolates of K. pneumoniae showed homology with strains in gene bank. The results of phylogenetic study revealed that the local isolates of K. pneumoniae of variable origins, these confirm their importance as primary pathogens of many diseases due to their widespread in the environment and their ability to infect humans and different animals.
Received: 16/1/2025
Accepted: 27/4/2025
Published: 31/7/2026
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