Genotyping of MC4R gene (G923>T) and its association with productive performance and carcass traits in Ross 308
DOI:
https://doi.org/10.36103/faatqh91Keywords:
broiler chickens,, growth traits,, melanocortin-4 gene, , SNP.Abstract
This study was aimed to select MC4R gene as a candidate gene for growth and carcass traits in Ross 308. The productive characteristics were studied weekly for 42 days for 100 chicks numbered individually. Blood samples were collected from chickens at 21 days old, Genotypes of MC4R were obtained using PCR products sequence. In this study, DNA sequencing data for the MC4R gene was detected technique SNP(G923>T) (1050bp) with three genotype appeared as (GG- GT- TT). However, the results show that a highly significant differences (P<0.01) between the MC4R genotype and body weight, weight gain, leg length and drumstick weight, with a significantly differences (P<0.05) in all of breast depth, drumstick length, carcass weight, back weight, weight of wings and abdominal fat weight. There was no variation detected in body length, breast circumference, drumstick circumference, breast, Neck, Liver, Heart and Gizzard weight. The MC4R gene SNP (G923>T) could be considered as a candidate marker gene for the early chicken selection program for growth and some carcass traits. The abstract must be no longer than 250 words and must clearly emphasise the objective of the study.
Received: 16/5/2024
Accepted: 14/8/2024
Published: 30/6/2026
References
Abdulkareem, A.A., Faraj S.H., Majeed M.S., Putra, W.P., & Harahap, R.S. 2025. Association analysis of polymorphisms in PLIN1 gene with the body weight of ross308 broiler chickens. Advances in Animal and Veterinary Sciences. 13(7): 1394-1400 https://dx.doi.org/10.17582/journal.aavs/2025/13.7.1394.1400 DOI: https://doi.org/10.17582/journal.aavs/2025/13.7.1394.1400
Aboelhassan, DM., Darwish, HR., Mansour, H., Abozaid, H., Ghaly, IS., Radwan, HA., Hassan ER., & Farag, IM. 2023. Polymorphisms and expressions of ADSL, MC4R and CAPN1 genes and their effects on economic traits in Egyptian chicken breeds. Molecular Biology Reports. 10;51(1):4. https://doi.org/10.1007/s11033-023-08999-w DOI: https://doi.org/10.1007/s11033-023-08999-w
Aggag, S., & El-Sabrout, K. 2018. Polymorphism of the melanocortin receptor gene and its association with egg production traits in Lohmann Brown chickens. 50(1): 317-323. https://doi.org/10.2298/GENSR1801317A DOI: https://doi.org/10.2298/GENSR1801317A
Al-jburi, A., & Senkal, R. 2023. Detection of MC4R Gene Genotypes and a Relationship SNP13 to Productive and Reproductive Performance in Iraqi Local Goats and Shami Goats. IOP Conference Series: Earth and Environmental Science. 1252: 012130. https://doi.org/10.1088/1755-1315/1252/1/012130 DOI: https://doi.org/10.1088/1755-1315/1252/1/012130
Aviagen catalogue. 2022. broiler guide. Aviagen, www.aviagen.com.
Bo, H., Hung, N., Manh, T., Vinh, N., & Luc, D. 2022. Additive and Dominance Effects of MC4R and PIT1 Polymorphisms on Production and Carcass Traits in Duroc Pigs. Vietnam Journal of Agricultural Sciences. 5(4): 1638-1644. https://doi.org/10.31817/vjas.2022.5.4.03 DOI: https://doi.org/10.31817/vjas.2022.5.4.03
Cawley, NX., Li, Z., & Loh, Y.P. 2016. 60 YEARS OF POMC: biosynthesis, trafficking, and secretion of pro-opiomelanocortin-derived peptides. Journal of Molecular Endocrinology. 56: 77–97.
https://doi.org/10.1530/JME-15-0323. DOI: https://doi.org/10.1530/JME-15-0323
Chau, H., Tra, T. Huong, D.T. Hang, D.T. Hang, L., Tam, V.T., Dung, D., & Thong, H. 2022. Effect of dietary SID methionine on growth performance, carcass and meat quality, myogenic gene expression in fast and slow growing coloured chickens. Advances in Animal and Veterinary Sciences. 10(3):529–536. https://doi.org/10.17582/journal.aavs/2022/10.3.529.536 DOI: https://doi.org/10.17582/journal.aavs/2022/10.3.529.536
Dale, N. 1994. National Research Council Nutrient Requirements of Poultry - Ninth Revised Edition. The Journal of Applied Poultry Research. 3. 101-101. https://doi.org/10.1093/japr/3.1.101 DOI: https://doi.org/10.1093/japr/3.1.101
Dores, R.M., & Lecaude, S. 2005. Trends in the evolution of the proopiomelanocortin gene. Gen Comp Endocrinol, 142(1-2):81-93. https://doi.org/10.1016/j.ygcen.2005.02.003 DOI: https://doi.org/10.1016/j.ygcen.2005.02.003
Duncan, D.B. 1955. Multiple Rang and Multiple F-test. Biometrics. 11: 4-42. DOI: https://doi.org/10.2307/3001478
Fathi, M., Hashemi, A., Elyasei, G., & Ghobayi, Z. 2018. Association analysis between genetic diversity of the melanocortin4 receptor (MC4R) gene and production traits in Turkey. Genetika, 50 (2): 617-626. https://doi.org/10.2298/GENSR1802617F DOI: https://doi.org/10.2298/GENSR1802617F
Fontanesi, L., Dall Olio, S., Spaccapaniccia, E., Scotti, E., Fornasini, D., Frabetti, A., & Russo, V.. 2012. A single nucleotide polymorphism in the rabbit growth hormone (GH1) gene is associated with market weight in a commercial rabbit population. Livestock Science. 147 (1-3): 84–88.
https://doi.org/10.1016/j.livsci.2012.04.006. DOI: https://doi.org/10.1016/j.livsci.2012.04.006
Hekmat, H., & Abdulkareem, A. 2026. Genetic Polymorphism of ASB15 gene and Its Relationship with Productive Performance, Growth Traits, and Skeletal Muscles in Rose 308 broiler chicken Chickens. University of Thi-Qar Journal of Agricultural Research, 15(1): 410-420. https://doi.org/10.54174/ebsx5618 DOI: https://doi.org/10.54174/ebsx5618
Hosnedlova, B., Vernerova, K., Kizek, R., Bozzi, R., Kadlec, J., Curn, V., Kouba, F., Fernandez, C., Machander, V., & Horna, H. 2020. Associations Between IGF1, IGFBP2 and TGFß3 Genes Polymorphisms and Growth Performance of Broiler Chicken Lines. Animals, 10(5): 800. https://doi.org/10.3390/ani10050800 DOI: https://doi.org/10.3390/ani10050800
Khumpeerawat, P., Duangjinda, M., & Phasuk, Y. 2021. Factors affecting gene expression associated with the skin color of black-bone chicken in Thailand. Poultry science, 100 (11): 101440. https://doi.org/10.1016/j.psj.2021.101440 DOI: https://doi.org/10.1016/j.psj.2021.101440
Li, C., & Li, H. 2006. Association of MC4R gene polymorphisms with growth and body composition traits in chicken. Asian Australasian Journal of Animal Sciences. 19:763–768. https://doi.org/10.5713/ajas.2006.763. DOI: https://doi.org/10.5713/ajas.2006.763
Musa, H. H., Chen, G. H., Cheng, J. H., Li B. C., & Mekki. D. M. 2006. study on carcass characteristics of chicken breeds raised under the intensive condition. International Journal of Poultry Science. 5 :530-533. DOI: 10.3923/ijps.2006.530.533 DOI: https://doi.org/10.3923/ijps.2006.530.533
Nie, Q., Fang, M., Xie, L., Zhou, M., Liang, Z., Luo, Z., Wang, g., Bi,W., Liang, C., Zhang, W., & Zhang, X. 2008. The PIT1 gene polymorphisms were associated with chicken growth traits. BMC Genetics. 9, 20 https://doi.org/10.1186/1471-2156-9-20 DOI: https://doi.org/10.1186/1471-2156-9-20
Radwan, H., Ateya, A., Abo Elfadl, E., Sakr, Sh., Darwish, R., & El- Desoky, A.. 2023. Efficiency of leptin gene polymorphisms in the evaluation of the growth performance and carcass measurements of v-line and Baladi black rabbits. Genetika, 55 (1): 319-338. https://doi.org/10.2298/GENSR2301319R. DOI: https://doi.org/10.2298/GENSR2301319R
SAS. 2012. Statistical Analysis Software. User’s Guide for Personal Computers, Release 6.12. SAS Institute Inc. Cary, NC, USA.
Schaefer, L. R. 2004. Application of random regression models in animal breeding. Livest. Prod. Sci., 16. 335-348. https://doi.org/10.1016/S03016226(03)00151-9
Sharma, P, Bottje, W., & Okimoto, R. 2008. Polymorphisms in uncoupling protein, melanocortin 3 receptor, melanocortin 4 receptor, and proopiomelanocortin genes and association with production traits in a commercial broiler line. The Journal of Poultry Science . 87(10), 2073–2086. https://doi.org/10.3382/ps.2008-00060. DOI: https://doi.org/10.3382/ps.2008-00060
Sun, J., Tan, X., Yang, X., Bai, L., Kong, F., Zhao, G., Wen, J., & Liu, R. 2022. Identification of candidate genes for meat color of chicken by combing selection signature analyses and differentially expressed genes. Genes, 13(2):307. https://doi.org/10.3390/genes13020307 6. DOI: https://doi.org/10.3390/genes13020307
Thu, N. T., Hung, L. T., Lan, L. T., Phong, N. H., Loc, H. T., Anh, L. H., & Ngu, N. T. 2021. The Association between POU1F1 gene polymorphisms and growth as well as Carcass Traits of Noi Native Chickens. The Journal of Advanced Veterinary Research, 11(1): 36-40. https://advetresearch.com/index.php/AVR/article/view/625
Wang, Y., Y. Su, X. Jiang, Y. Liu, X. Li, Z. Zhang, H. Du & Q. Zhu. 2009. Study on association of single nucleotide polymorphism of MC3R and MC4R genes with carcass and meat quality traits in chicken. The Journal of Poultry Science, 46: 180-187. https://doi.org/10.2141/JPSA.46.180 DOI: https://doi.org/10.2141/jpsa.46.180
Xuemei, Q., Ning, L., Xuemei, D., Xingbo, Z., Qingyong, M., & Xiuli, W. 2006. The single nucleotide polymorphisms of chicken melanocortin-4 receptor (MC4R) gene and their association analysis with carcass traits. Science in China Series C: Life Science, 49(6): 560-566.
https://doi.org/10.1007/s11427-006-2029-7 DOI: https://doi.org/10.1007/s11427-006-2029-7
Yi, T., Yang, LK., Ruan, GL., Yang, DQ., & Tao, Y.X. 2018. Melanocortin-4 receptor in swamp eel (Monopterus albus): Cloning, tissue distribution, and pharmacology. Gene. 15(678):79-89 https://doi.org/10.1016/j.gene.2018.07.056 DOI: https://doi.org/10.1016/j.gene.2018.07.056
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