THE EFFECT OF BIOFERTILIZATION, ORGANIC NUTRIENT SPRAYING AND GLUTAMIC ACID ON SOME LETTUCE TRAITS
DOI:
https://doi.org/10.36103/2xrwk829Keywords:
azospirillum, Lactuca sativa, reduced mineral fertilizer, mycorrhizaAbstract
A field experiment was conducted over two agricultural seasons, in a field of the Organic Fertilizers Project located in the Bani Zaid district of Shatra, under the Directorate of Agriculture of Dhi Qar. The factorial experiment included three factors conducted within a Randomized Complete Block Design (R.C.B.D) with three replications, 36 treatments per replicate. The study aimed to evaluate the effects of reducing mineral fertilizer application by 50% on lettuce (Lactuca sativa L.) growth, focusing on the combined impact of biofertilization with mycorrhiza and Azospirillum, along with foliar applications of an organic nutrient solution (Forteorgan) and glutamic acid. Conducted over two agricultural seasons (2022-2023 and 2023-2024). The results indicated that the combined treatment of mycorrhiza and Azospirillum inoculation, coupled with foliar applications of 4 ml L-1 Forteorgan and 500 mg L-1 glutamic acid, significantly enhanced leaf nitrogen percent in both seasons. Additionally, this combination improved leaf phosphorus and potassium percent, as well as total chlorophyll concentration, suggesting that integrating biofertilizers with reduced mineral fertilizer application can effectively promote nutrient uptake and photosynthesis in lettuce plants.
Received: 26/8/2024
Accepted: 29/12/2024
Published: 30/6/2026
References
Ahammed, G. J., & Hajiboland, R. (2024). Introduction to arbuscular mycorrhizal fungi and higher plant symbiosis: characteristic features, functions, and applications. In Arbuscular mycorrhizal fungi and higher plants: fundamentals and applications (pp. 1-17). Singapore: Springer Nature Singapore. doi.org/10.1007/978-981-99-8220-2_1. DOI: https://doi.org/10.1007/978-981-99-8220-2_1
Ahmed, Z. F., Alnuaimi, A. K., Askri, A., & Tzortzakis, N. (2021). Evaluation of Lettuce (Lactuca sativa L.) production under hydroponic system: Nutrient solution derived from fish waste vs. Inorganic nutrient solution. Horticulturae, 7(9), 292. doi.org/10.3390/horticulturae7090292. DOI: https://doi.org/10.3390/horticulturae7090292
Al-Atawi, N. H. M. (2021(. Effect of Two Types of Mycorrhizal Fungi, Number of Sprays of Liquid Humic Acid, and Concentrations of Licorice Extract on the Growth and Yield of Okra (Abelmoschus esculentus L.). Ph.D. Dissertation. College of Agriculture. Al-Muthanna University. Iraq. pp: 126.
Al-Chalabi, A. T. M., Ibraheem, F. F., Hussein, A. M., & Alsawaf, A. (2023, December). Comparison the effect of an organic nutrient (Nektafol) under production and open field condition on the growth and yield of lettuce. In IOP Conference Series: Earth and Environmental Science (Vol. 1262, No. 4, p. 042003). IOP Publishing. DOI:10.1088/1755-1315/1262/4/042003 DOI: https://doi.org/10.1088/1755-1315/1262/4/042003
Ali, N. S., M. M. Alawi, & N. H. Majeed (2022). Rhizosphere Management and Agricultural Sustainability. University of Baghdad. College of Agricultural Engineering Sciences. p. 325. Iraq.
Fall, A. F. G. Nakabonge. J. Ssekandi et al. 2023. combined effects of indigenous arbuscular mycorrhizal fungi (AMF) and NPK fertilizer on growth and Yields of Maize and Soil Nutrient Availability. J. Sustainability. Vol. 15 (3): DOI: https://doi.org/10.3390/su15032243
pp: 2243.. doi.org/10.3390/su15032243.
Al-Mashhadany, A. H. (2023). Effect of fertilizers starter solutions on growth and production of broccoli (Brassica oleracea var. italica). Research on Crops, 24(1),119-122. doi: 10.31830/2348-7542.2023.ROC-11155 DOI: https://doi.org/10.31830/2348-7542.2023.ROC-11155
Al-Mharib, M. Z., Attalah, A. M., & Ali, A. B. (2019). Effect of adding humic acid and phosphate fertilizer levels on growth and yield of lettuce. Journal of Agriculture and Veterinary Science, 12(4), 12-15.
https://doi.org/10.9790/2380-1204011215.
Al-Asady, M. H. S. (2019). GenStat For Analyzing Agricultural Experiments. Al-Qasim Green University – Agriculture College. Dar-Alwarith printing & publishing. pp: 304.
Alshegaihi, R. M., Alatawi, A., & Alenezi, M. A. (2024). Ameliorative effects of plant growth promoting rhizobacteria and arbuscular mycorrhizal fungi on cu stress in maize (Zea mays L.) with a focus on oxidative damage, antioxidant responses, and gene expression. Journal of soil science and plant nutrition, 24(2), 2437-2455. DOI: https://doi.org/10.1007/s42729-024-01662-8
doi.org/10.1007/s42729-024-01662-8.
AL-Ubaidy, R. M., and A. K. N. AL-Zaidy. (2017). Effect of adding wheat peat and spraying with extract and organic nutrient vegeamino in red cabbage leaves content of certain nutrients. IOSR Journal of Agriculture and Veterinary Science 10 (8): 6–10. doi:10.9790/2380-1008030610.
Al-Ubaidy, R. M., H. H. Al-Umrani, and L. A. Al-Shmarmy. 2020. Response of celery to addition of nitrogen and spray with salicylic acid in the leaves content of nutrients. IOP Conference Series: Earth and Environmental Science 553: 012007. DOI:10.1088/1755-1315/553/1/012007. DOI: https://doi.org/10.1088/1755-1315/553/1/012007
Al-Zaidi, R. J. A. (2021). Effect of Fish Farm Wastewater and Spraying with hornwort extract on growth and yield of Lettuce. Master’s Thesis. College of Agricultural Engineering Sciences. University of Baghdad. Iraq. pp : 90.
Ammar, G. A. G. (2024). Plant beneficial symbionts: charming members in the phytomicrobiome community. future perspectives of medical, Pharmaceutical and Environmental Biotechnology 1 (1): 19–30. doi: 10.21608/fpmpeb.2024.266342.1007. DOI: https://doi.org/10.21608/fpmpeb.2024.266342.1007
Avio, L., Sbrana, C., Giovannetti, M., & Frassinetti, S. (2017). Arbuscular mycorrhizal fungi affect total phenolics content and antioxidant activity in leaves of oak leaf lettuce varieties. Scientia Horticulturae, 224, 265-271. doi.org/10.1016/j.scienta.2017.06.022. DOI: https://doi.org/10.1016/j.scienta.2017.06.022
Balcı, G. (2023). The Effect of the Application of Mycorrhiza on Vegetative Growth, Mineral Element Intake, and Some Biochemical Characteristics of Strawberry Seedlings under Lime Stress. Horticultural Studies, 40(2), 62-71. https://doi.org/10.16882/hortis.1330523. DOI: https://doi.org/10.16882/hortis.1330523
Bhantana, P., Rana, M. S., Sun, X. C., Moussa, M. G., Saleem, M. H., Syaifudin, M., ... & Hu, C. X. (2021). Arbuscular mycorrhizal fungi and its major role in plant growth, zinc nutrition, phosphorous regulation and phytoremediation. Symbiosis, 84(1), 19-37. doi.org/10.1007/s13199-021-00756-6. DOI: https://doi.org/10.1007/s13199-021-00756-6
Cela, F., Avio, L., Giordani, T., Vangelisti, A., Cavallini, A., Turrini, A., ... & Incrocci, L. (2022). Arbuscular mycorrhizal fungi increase nutritional quality of soilless grown lettuce while overcoming low phosphorus supply. Foods, 11(22), 3612. https://doi.org/10.3390/foods11223612. DOI: https://doi.org/10.3390/foods11223612
Chatzistathis, T., V. Kavvadias, T. Sotiropoulos, & I. E. Papadakis. (2021). Organic fertilization and tree orchards. Journal of Agriculture 11 (8): 692. doi.org/10.3390/agriculture11080692. DOI: https://doi.org/10.3390/agriculture11080692
Da Silva Oliveira, C. E., Jalal, A., Vitória, L. S., Giolo, V. M., Oliveira, T. J. S. S., Aguilar, J. V., & Filho, M. C. M. T. (2023). Inoculation with Azospirillum brasilense strains AbV5 and AbV6 increases nutrition, chlorophyll, and leaf yield of hydroponic lettuce. Plants, 12(17), 3107. DOI: https://doi.org/10.3390/plants12173107
doi.org/10.3390/plants12173107
Dakora, F. D.(2003). Defining new roles for plant and rhizobial molecules in sole and mixed plant cultures involving symbiotic legumes. New Phytologist, 158(1),39-49. https://doi.org/10.1046/j.1469-8137.2003.00725.x. DOI: https://doi.org/10.1046/j.1469-8137.2003.00725.x
De Gregorio, M. A., Zengin, G., Alp-Turgut, F. N., Elbasan, F., Ozfidan-Konakci, C., Arikan, B., & Lucini, L. (2023). Glutamate, humic acids and their combination modulate the phenolic profile, antioxidant traits, and enzyme-inhibition properties in lettuce. Plants, 12(9), 1822. https://doi.org/10.3390/ plants12091822. DOI: https://doi.org/10.3390/plants12091822
Ekinci, M., Yıldırım, E., Turan, M., & Kul, R. (2020). Organic Fertilizers Improved Plant Growth and Mineral Content of Lettuce (Lactuca sativa L.). Erciyes Tarım ve Hayvan Bilimleri Dergisi, 3(2), 1-5.
Franzoni, G., Cocetta, G., & Ferrante, A. (2021). Effect of glutamic acid foliar applications on lettuce under water stress. Physiology and Molecular Biology of Plants, 27(5), 1059-1072. doi.org/10.1007/s12298-021-00984-6. DOI: https://doi.org/10.1007/s12298-021-00984-6
Frasetya, B., Taofik, A., & Nasrulloh, L. (2021, March). The effectiveness of the application method of liquid organic fertilizer in different concentration on lettuce. In IOP Conference Series: Earth and Environmental DOI: https://doi.org/10.1088/1755-1315/694/1/012024
Downloads
Published
Issue
Section
License
Copyright (c) 2026 Ayad Ali Hunaish Al-Samah , Ridha Mustafa Abdul-Hussein Al-Ubaidy

This work is licensed under a Creative Commons Attribution 4.0 International License.

2.jpg)
