IDENTIFICATION OF FUSARIUM OXYSPORUM F.SP. NIVEUM RACES CAUSING VASCULAR WILT DISEASE IN WATERMELON PLANTS IN WESTERN IRAQ

Authors

  • Taha D. Kazem Al-Aradi Department of Plant Protection, College of Agricultural Engineering Sciences, University of Baghdad, Iraq
  • Tariq A. Kareem Department of Plant Protection, College of Agricultural Engineering Sciences, University of Baghdad, Iraq

DOI:

https://doi.org/10.36103/yxbvrk17

Keywords:

Citrullus lanatus (Charleston Gray), Fusarium oxysporum, Molecular, Pathogenicit

Abstract

This study aimed to identify and classify races of Fusarium oxysporum f.sp. niveum, the causal agent of Fusarium wilt in watermelon, using precise molecular methods. Nine fungal isolates were collected from different agricultural regions in Iraq and identified using Polymerase Chain Reaction (PCR) techniques with specialized primers targeting specific regions of the fungal DNA. After molecular confirmation, the pathogenicity of these isolates was tested on five hybrids of the Charleston Gray watermelon cultivar in a laboratory experiment, helping to assess the pathogenic effects of the isolates. The results showed significant variation in the pathogenic abilities of the isolates, with Race 3 being the most aggressive, displaying infection rates ranging from 80-100% and infection severity between 76.6-96.5%. This study highlights the importance of using molecular techniques for accurately and rapidly identifying pathogenic races, which can aid in developing effective disease management strategies and reducing agricultural crop losses.

References

Amaradasa, B.S., K. Beckham, N. Dufault, T. Sanchez, T.S. Ertek, F. Iriarte, M. Paret, & P. Ji. 2018. First report of Fusarium oxysporum f. sp. niveum Race 3 causing wilt of watermelon in Florida, USA. Plant Disease 102, (5): 1029. https://doi.org/10.1094/PDIS-10-17-1649-PDN. DOI: https://doi.org/10.1094/PDIS-10-17-1649-PDN

Bruton, B.D., W.W. Fish, & D.B. Langston. 2008. First report of fusarium wilt caused by Fusarium oxysporum f. sp. niveum Race 2 in Georgia watermelons. Plant Disease 92, .(6): 983.

https://doi.org/10.1094/PDIS-92-6-0983B. DOI: https://doi.org/10.1094/PDIS-92-6-0983B

Chang, W., H. Li, H. Chen, F. Qiao, & H. Zeng. 2020. Identification of mimp-associated effector genes in Fusarium oxysporum f. sp. cubense Race 1 and Race 4 and virulence confirmation of a candidate effector gene. Microbiological Research 232:(126375). https://doi.org/10.1016/j.micres.2019.126375. DOI: https://doi.org/10.1016/j.micres.2019.126375

Elmstrom, G.W., & D.L. Hopkins. 1981. Resistance of watermelon cultivars to fusarium wilt. Plant Disease 65, .(10): 825–827. https://doi.org/10.1094/PD-65-825. DOI: https://doi.org/10.1094/PD-65-825

Fernández-Herrera, E., T.E. González-Soto, I. I. R.B.I. Iliana, & R. Bustos. 2021. Fusarium oxysporum f. sp. niveum: causal agent of vascular withering of watermelon. Agro Productividad 14, . (5). https://doi.org/10.32854/agrop.v14i05.1924. DOI: https://doi.org/10.32854/agrop.v14i05.1924

Freeman, S., & R.J. Rodriguez. 1993. A Rapid inoculation technique for assessing pathogenicity of Fusarium oxysporum f. sp. niveum and F. o. melonis on Cucurbits. Plant Disease 77, .(12): 1198–1201. DOI: https://doi.org/10.1094/PD-77-1198

Fulton, J.C., M.A. Cullen, K. Beckham, T. Sanchez, Z. Xu, P. Stern, G. Vallad, G. Meru, C. McGregor, & N.S. Dufault. 2021. A contrast of three inoculation techniques used to determine the race of unknown Fusarium oxysporum f. sp. niveum Isolates. (JoVE) Journal of Visualized Experiments,u.(176): e63181.

https://doi.org/10.3791/63181. DOI: https://doi.org/10.3791/63181

Guadet, J., J. Julien, J. F. Lafay, & Y. Brygoo. 1989. Phylogeny of some fusarium species, as determined by large-subunit rRNA sequence comparison. Molecular Biology and Evolution 6, .(3): 227–242. https://doi.org/10.1093/oxfordjournals.molbev.a040548. DOI: https://doi.org/10.1093/oxfordjournals.molbev.a040548

Hirano, Y., & T. Arie. 2006. PCR-Based differentiation of Fusarium oxysporum ff. sp. Lycopersici and Radicis-Lycopersici and Races of F. oxysporum f. sp. Lycopersici. Journal of General Plant Pathology 72. 273–283. DOI: https://doi.org/10.1007/s10327-006-0287-7

Hudson, O., J. C. Fulton, A.K. Dong, N.S. Dufault, & M. E. Ali. 2021. Fusarium oxysporum f. sp. niveum molecular diagnostics past, present and future. International Journal of Molecular Sciences 22, .(18): 9735. https://doi.org/10.3390/ijms22189735. DOI: https://doi.org/10.3390/ijms22189735

Kadhim, J.H., A.E. Mohammed, S.A.A.J. Allwbawi, & A.A. Mohammed. 2019. The effect of MgSO4 on behavior of the pathogenic fungus, Fusarium solani and the rate of seedlings damping-off disease on sesame. Biochemical and Cellular Archives 19, .(2): 3047–3053. https://doi.org/10.35124/bca.2019.19.2.3047.

Keinath, A.P., V.B. DuBose, M.M. Katawczik, & W.P. Wechter. 2020. Identifying races of Fusarium oxysporum f. sp. niveum in South Carolina recovered from watermelon seedlings, plants, and field soil, Plant Disease, 104, .(9): 2481–2488. https://doi.org/10.1094/PDIS-11-19-2385-RE. DOI: https://doi.org/10.1094/PDIS-11-19-2385-RE

Kleczewski, N. M., & D.S. Egel. 2011. A Diagnostic Guide for Fusarium Wilt of Watermelon. Plant Health Progress 12, .(1): 27.

https://doi.org/10.1094/PHP-2011-1129-01-DG. DOI: https://doi.org/10.1094/PHP-2011-1129-01-DG

Kyriacou, M.C., D. I. Leskovar, G. Colla, & Y. Rouphael. 2018. Watermelon and melon fruit quality: the genotypic and agro-environmental factors implicated. Scientia Horticulturae 234: 393–408. DOI: https://doi.org/10.1016/j.scienta.2018.01.032

Lal, D., D. Dev, S. Kumari, S. Pandey, Aparna, N. Sharma, S. Nandni, R. K. Jha, & A. Singh. 2024. fusarium wilt pandemic: current understanding and molecular perspectives. Functional & Integrative Genomics 24, .(2): 41. https://doi.org/10.1007/s10142-024-01319-w DOI: https://doi.org/10.1007/s10142-024-01319-w

Manivannan, A., E.S. Lee, K. Han, H.E. Lee, & D. S. Kim. 2020. Versatile nutraceutical potentials of watermelon—A modest fruit loaded with pharmaceutically valuable phytochemicals. Molecules 25, .(22):5258. https://doi.org/10.3390/molecules25225258. DOI: https://doi.org/10.3390/molecules25225258

Martyn, R. D. 2014. Fusarium wilt of watermelon: 120 years of research. In Horticultural Reviews, 42,(No) 349–442. https://doi.org/10.1002/9781118916827.ch07. DOI: https://doi.org/10.1002/9781118916827.ch07

Martyn, R.D., & B.D. Bruton. 1989. An initial survey of the United States for races of Fursarium oxysporum f. sp. niveum. HortScience 24, .(4): 696–698. https://doi.org/10.21273/HORTSCI.24.4.696. DOI: https://doi.org/10.21273/HORTSCI.24.4.696

Martyn, R.D., & D. Netzer. 1991. Resistance to Races 0, 1, and 2 of fusarium wilt of watermelon in Citrullus sp. PI-296341-FR. HortScience 26, .(4): 429–432. DOI: https://doi.org/10.21273/HORTSCI.26.4.429

Najem, Hanan W., & T.A. Kareem. 2018. Morphological and molecular identification of Monosporascus cannonballus causal agent of melon root rot and plant decline in Iraq. J. Bio. Env. Sci. 13, .(6): 83–88.

Naz, A., M.S. Butt, M.T. Sultan, M.M.N. Qayyum, & R.S. Niaz. 2014. Watermelon lycopene and allied health claims. EXCLI Journal 13(No): 650.

Niu, X., X. Zhao, K.S. Ling, A. Levi, Y. Sun, & M. Fan. 2016. The fonsix6 gene acts as an avirulence effector in the Fusarium oxysporum f. sp. niveum-Watermelon Pathosystem. Scientific Reports 6, .(1): 28146. https://doi.org/10.1038/srep28146. DOI: https://doi.org/10.1038/srep28146

Petkar, A., K. Harris-Shultz, H. Wang, M.T. Brewer, L. Sumabat, & P. Ji. 2019. Genetic and phenotypic diversity of Fusarium oxysporum f. sp. niveum populations from watermelon in the southeastern United States. PloS One 14,.(7): e0219821. https://doi.org/10.1371/journal.pone.0219821. DOI: https://doi.org/10.1371/journal.pone.0219821

Rahman, M.Z., K. Ahmad, Y. Siddiqui, N. Saad, T.G. Hun, E.M. Hata, O. Rashed, M.I. Hossain, & A.B. Kutawa. 2021. First report of fusarium wilt disease on watermelon caused by Fusarium oxysporum f. sp. niveum in Malaysia. Plant Disease 105, .(12): 4169. https://doi.org/10.1094/PDIS-04-21-0780-PDN. DOI: https://doi.org/10.1094/PDIS-04-21-0780-PDN

Soleha, S., A. Muslim, S. Suwandi, S. Kadir, & R. Pratama. 2022. The identification and pathogenicity of fusarium oxysporum causing acacia seedling wilt disease. Journal of Forestry Research 33, .(2): 711–719. https://doi.org/10.1007/s11676-021-01355-3. DOI: https://doi.org/10.1007/s11676-021-01355-3

Tan, C.K., W. Sun, D. Borovac, & N. Tansu. 2016. Large optical gain AlInN-Delta-GaN quantum well for deep ultraviolet emitters. Scientific Reports 6, .(1): 22983. https://doi.org/10.1038/srep22983. DOI: https://doi.org/10.1038/srep22983

Vargas-Arispuro, I., I.I. Ramírez-Bustos, A.A. Arratia-Castro, D. Bárcena-Santana, & E. Fernández-Herrera. 2023. First report of Fusarium oxysporum f. sp. niveum Race 1 as causal agent of vascular wilt of watermelon in Mexico. Revista Chapingo. Serie Horticultura 29, .(3): 47–57. https://doi.org/10.5154/r.rchsh.2022.11.014. DOI: https://doi.org/10.5154/r.rchsh.2022.11.014

Zhou, X.G., & K.L. Everts. 2003. Races and inoculum density of Fusarium oxysporum f. sp. niveum in commercial watermelon fields in Maryland and Delaware. Plant Disease 87, (6): 692–698. https://doi.org/10.1094/PDIS.2003.87.6.692. DOI: https://doi.org/10.1094/PDIS.2003.87.6.692

Downloads

Published

2026-06-30

Issue

Section

Articles

How to Cite

Al-Aradi , T., & Kareem, T. (2026). IDENTIFICATION OF FUSARIUM OXYSPORUM F.SP. NIVEUM RACES CAUSING VASCULAR WILT DISEASE IN WATERMELON PLANTS IN WESTERN IRAQ . IRAQI JOURNAL OF AGRICULTURAL SCIENCES, 57(6), 1771-1782. https://doi.org/10.36103/yxbvrk17