IDENTIFICATION OF FUSARIUM OXYSPORUM F.SP. NIVEUM RACES CAUSING VASCULAR WILT DISEASE IN WATERMELON PLANTS IN WESTERN IRAQ
DOI:
https://doi.org/10.36103/yxbvrk17Keywords:
Citrullus lanatus (Charleston Gray), Fusarium oxysporum, Molecular, PathogenicitAbstract
This study aimed to identify and classify races of Fusarium oxysporum f.sp. niveum, the causal agent of Fusarium wilt in watermelon, using precise molecular methods. Nine fungal isolates were collected from different agricultural regions in Iraq and identified using Polymerase Chain Reaction (PCR) techniques with specialized primers targeting specific regions of the fungal DNA. After molecular confirmation, the pathogenicity of these isolates was tested on five hybrids of the Charleston Gray watermelon cultivar in a laboratory experiment, helping to assess the pathogenic effects of the isolates. The results showed significant variation in the pathogenic abilities of the isolates, with Race 3 being the most aggressive, displaying infection rates ranging from 80-100% and infection severity between 76.6-96.5%. This study highlights the importance of using molecular techniques for accurately and rapidly identifying pathogenic races, which can aid in developing effective disease management strategies and reducing agricultural crop losses.
Received: 22/8/2024
Accepted: 6/11/2024
Published: 30/6/2026
References
Amaradasa, B.S., K. Beckham, N. Dufault, T. Sanchez, T.S. Ertek, F. Iriarte, M. Paret, & P. Ji. 2018. First report of Fusarium oxysporum f. sp. niveum Race 3 causing wilt of watermelon in Florida, USA. Plant Disease 102, (5): 1029. https://doi.org/10.1094/PDIS-10-17-1649-PDN. DOI: https://doi.org/10.1094/PDIS-10-17-1649-PDN
Bruton, B.D., W.W. Fish, & D.B. Langston. 2008. First report of fusarium wilt caused by Fusarium oxysporum f. sp. niveum Race 2 in Georgia watermelons. Plant Disease 92, .(6): 983.
https://doi.org/10.1094/PDIS-92-6-0983B. DOI: https://doi.org/10.1094/PDIS-92-6-0983B
Chang, W., H. Li, H. Chen, F. Qiao, & H. Zeng. 2020. Identification of mimp-associated effector genes in Fusarium oxysporum f. sp. cubense Race 1 and Race 4 and virulence confirmation of a candidate effector gene. Microbiological Research 232:(126375). https://doi.org/10.1016/j.micres.2019.126375. DOI: https://doi.org/10.1016/j.micres.2019.126375
Elmstrom, G.W., & D.L. Hopkins. 1981. Resistance of watermelon cultivars to fusarium wilt. Plant Disease 65, .(10): 825–827. https://doi.org/10.1094/PD-65-825. DOI: https://doi.org/10.1094/PD-65-825
Fernández-Herrera, E., T.E. González-Soto, I. I. R.B.I. Iliana, & R. Bustos. 2021. Fusarium oxysporum f. sp. niveum: causal agent of vascular withering of watermelon. Agro Productividad 14, . (5). https://doi.org/10.32854/agrop.v14i05.1924. DOI: https://doi.org/10.32854/agrop.v14i05.1924
Freeman, S., & R.J. Rodriguez. 1993. A Rapid inoculation technique for assessing pathogenicity of Fusarium oxysporum f. sp. niveum and F. o. melonis on Cucurbits. Plant Disease 77, .(12): 1198–1201. DOI: https://doi.org/10.1094/PD-77-1198
Fulton, J.C., M.A. Cullen, K. Beckham, T. Sanchez, Z. Xu, P. Stern, G. Vallad, G. Meru, C. McGregor, & N.S. Dufault. 2021. A contrast of three inoculation techniques used to determine the race of unknown Fusarium oxysporum f. sp. niveum Isolates. (JoVE) Journal of Visualized Experiments,u.(176): e63181.
https://doi.org/10.3791/63181. DOI: https://doi.org/10.3791/63181
Guadet, J., J. Julien, J. F. Lafay, & Y. Brygoo. 1989. Phylogeny of some fusarium species, as determined by large-subunit rRNA sequence comparison. Molecular Biology and Evolution 6, .(3): 227–242. https://doi.org/10.1093/oxfordjournals.molbev.a040548. DOI: https://doi.org/10.1093/oxfordjournals.molbev.a040548
Hirano, Y., & T. Arie. 2006. PCR-Based differentiation of Fusarium oxysporum ff. sp. Lycopersici and Radicis-Lycopersici and Races of F. oxysporum f. sp. Lycopersici. Journal of General Plant Pathology 72. 273–283. DOI: https://doi.org/10.1007/s10327-006-0287-7
Hudson, O., J. C. Fulton, A.K. Dong, N.S. Dufault, & M. E. Ali. 2021. Fusarium oxysporum f. sp. niveum molecular diagnostics past, present and future. International Journal of Molecular Sciences 22, .(18): 9735. https://doi.org/10.3390/ijms22189735. DOI: https://doi.org/10.3390/ijms22189735
Kadhim, J.H., A.E. Mohammed, S.A.A.J. Allwbawi, & A.A. Mohammed. 2019. The effect of MgSO4 on behavior of the pathogenic fungus, Fusarium solani and the rate of seedlings damping-off disease on sesame. Biochemical and Cellular Archives 19, .(2): 3047–3053. https://doi.org/10.35124/bca.2019.19.2.3047.
Keinath, A.P., V.B. DuBose, M.M. Katawczik, & W.P. Wechter. 2020. Identifying races of Fusarium oxysporum f. sp. niveum in South Carolina recovered from watermelon seedlings, plants, and field soil, Plant Disease, 104, .(9): 2481–2488. https://doi.org/10.1094/PDIS-11-19-2385-RE. DOI: https://doi.org/10.1094/PDIS-11-19-2385-RE
Kleczewski, N. M., & D.S. Egel. 2011. A Diagnostic Guide for Fusarium Wilt of Watermelon. Plant Health Progress 12, .(1): 27.
https://doi.org/10.1094/PHP-2011-1129-01-DG. DOI: https://doi.org/10.1094/PHP-2011-1129-01-DG
Kyriacou, M.C., D. I. Leskovar, G. Colla, & Y. Rouphael. 2018. Watermelon and melon fruit quality: the genotypic and agro-environmental factors implicated. Scientia Horticulturae 234: 393–408. DOI: https://doi.org/10.1016/j.scienta.2018.01.032
Lal, D., D. Dev, S. Kumari, S. Pandey, Aparna, N. Sharma, S. Nandni, R. K. Jha, & A. Singh. 2024. fusarium wilt pandemic: current understanding and molecular perspectives. Functional & Integrative Genomics 24, .(2): 41. https://doi.org/10.1007/s10142-024-01319-w DOI: https://doi.org/10.1007/s10142-024-01319-w
Manivannan, A., E.S. Lee, K. Han, H.E. Lee, & D. S. Kim. 2020. Versatile nutraceutical potentials of watermelon—A modest fruit loaded with pharmaceutically valuable phytochemicals. Molecules 25, .(22):5258. https://doi.org/10.3390/molecules25225258. DOI: https://doi.org/10.3390/molecules25225258
Martyn, R. D. 2014. Fusarium wilt of watermelon: 120 years of research. In Horticultural Reviews, 42,(No) 349–442. https://doi.org/10.1002/9781118916827.ch07. DOI: https://doi.org/10.1002/9781118916827.ch07
Martyn, R.D., & B.D. Bruton. 1989. An initial survey of the United States for races of Fursarium oxysporum f. sp. niveum. HortScience 24, .(4): 696–698. https://doi.org/10.21273/HORTSCI.24.4.696. DOI: https://doi.org/10.21273/HORTSCI.24.4.696
Martyn, R.D., & D. Netzer. 1991. Resistance to Races 0, 1, and 2 of fusarium wilt of watermelon in Citrullus sp. PI-296341-FR. HortScience 26, .(4): 429–432. DOI: https://doi.org/10.21273/HORTSCI.26.4.429
Najem, Hanan W., & T.A. Kareem. 2018. Morphological and molecular identification of Monosporascus cannonballus causal agent of melon root rot and plant decline in Iraq. J. Bio. Env. Sci. 13, .(6): 83–88.
Naz, A., M.S. Butt, M.T. Sultan, M.M.N. Qayyum, & R.S. Niaz. 2014. Watermelon lycopene and allied health claims. EXCLI Journal 13(No): 650.
Niu, X., X. Zhao, K.S. Ling, A. Levi, Y. Sun, & M. Fan. 2016. The fonsix6 gene acts as an avirulence effector in the Fusarium oxysporum f. sp. niveum-Watermelon Pathosystem. Scientific Reports 6, .(1): 28146. https://doi.org/10.1038/srep28146. DOI: https://doi.org/10.1038/srep28146
Petkar, A., K. Harris-Shultz, H. Wang, M.T. Brewer, L. Sumabat, & P. Ji. 2019. Genetic and phenotypic diversity of Fusarium oxysporum f. sp. niveum populations from watermelon in the southeastern United States. PloS One 14,.(7): e0219821. https://doi.org/10.1371/journal.pone.0219821. DOI: https://doi.org/10.1371/journal.pone.0219821
Rahman, M.Z., K. Ahmad, Y. Siddiqui, N. Saad, T.G. Hun, E.M. Hata, O. Rashed, M.I. Hossain, & A.B. Kutawa. 2021. First report of fusarium wilt disease on watermelon caused by Fusarium oxysporum f. sp. niveum in Malaysia. Plant Disease 105, .(12): 4169. https://doi.org/10.1094/PDIS-04-21-0780-PDN. DOI: https://doi.org/10.1094/PDIS-04-21-0780-PDN
Soleha, S., A. Muslim, S. Suwandi, S. Kadir, & R. Pratama. 2022. The identification and pathogenicity of fusarium oxysporum causing acacia seedling wilt disease. Journal of Forestry Research 33, .(2): 711–719. https://doi.org/10.1007/s11676-021-01355-3. DOI: https://doi.org/10.1007/s11676-021-01355-3
Tan, C.K., W. Sun, D. Borovac, & N. Tansu. 2016. Large optical gain AlInN-Delta-GaN quantum well for deep ultraviolet emitters. Scientific Reports 6, .(1): 22983. https://doi.org/10.1038/srep22983. DOI: https://doi.org/10.1038/srep22983
Vargas-Arispuro, I., I.I. Ramírez-Bustos, A.A. Arratia-Castro, D. Bárcena-Santana, & E. Fernández-Herrera. 2023. First report of Fusarium oxysporum f. sp. niveum Race 1 as causal agent of vascular wilt of watermelon in Mexico. Revista Chapingo. Serie Horticultura 29, .(3): 47–57. https://doi.org/10.5154/r.rchsh.2022.11.014. DOI: https://doi.org/10.5154/r.rchsh.2022.11.014
Zhou, X.G., & K.L. Everts. 2003. Races and inoculum density of Fusarium oxysporum f. sp. niveum in commercial watermelon fields in Maryland and Delaware. Plant Disease 87, (6): 692–698. https://doi.org/10.1094/PDIS.2003.87.6.692. DOI: https://doi.org/10.1094/PDIS.2003.87.6.692
Downloads
Published
Issue
Section
License
Copyright (c) 2026 Taha D. Kazem Al-Aradi , Tariq A. Kareem

This work is licensed under a Creative Commons Attribution 4.0 International License.

2.jpg)
