PROTECTIVE ROLE OF TOPSIN M 70 FUNGICIDE AND SILICON OXIDE AGAINST WHITE MOLD DISEASE ON TWO CULTIVARS OF EGGPLANT
DOI:
https://doi.org/10.36103/9hvbs808Keywords:
Area under disease progress curve, Induced resistance, Silicon oxide, Topsin M 70, Sclerotinia sclerotiorumAbstract
This study was conducted at the Plant Protection Directorate / Technical Departments Complex in 2023 to identify the causal agent of white mold disease and evaluate the effectiveness of the fungicide Topsin M 70 and silicon oxide in controlling disease progression, infection rate, and severity. The results indicated that the Sclerotinia sclerotiorum (SS5) isolate exhibited the highest pathogenicity, with an infection rate of 100% and a severity of 82.5%. DNA electrophoresis analysis of the SS5 isolate revealed a single band with a molecular weight of 550 bp, showing 100% similarity to globally registered isolates in the NCBI gene bank. The nucleotide sequences were registered under accession number OR497817. The application of nano-silicon oxide (0.001 g/L), conventional silicon oxide (3 g/L), and the chemical fungicide Topsin M 70 (1 g/L) as foliar sprays on Solanum melongena cultivars Wasam and Barcelona exhibited protective effects. The chemical fungicide provided complete disease suppression, recording 0% infection rate and severity in both cultivars. Nano-silicon oxide resulted in an infection rate of 13.87% and severity of 5.44%, while conventional silicon oxide recorded 63.16% infection rate and 7.43% severity, compared to the control, which showed 50% infection rate and 25.69% severity. The area under the disease progression curve (AUDPC) was 130.95 and 143.21 in the control treatment, but it dropped to 0% with the application of the chemical fungicide.
Received: 27/12/2024
Accepted: 5/3/2025
Published: 30/6/2026
References
Abdelrhim, A. S., Mazrou, Y. S., Nehela, Y., Atallah, O. O., El-Ashmony, R. M., & Dawood, M. F. (2021). Silicon dioxide nanoparticles induce innate immune responses and activate antioxidant machinery in wheat against Rhizoctonia solani. Plants, 10(12), 2758. https://doi.org/10.3390/plants10122758 DOI: https://doi.org/10.3390/plants10122758
Abdul-Karim, E. K., Aljarah, N. S., & Ali, H. A. A. (2023). Molecular characterization of Neoscytalidium spp. the cause of wilting of branches and blackening of the stem. IOP Conference Series: Earth and Environmental Science, 1262(3), 032032. https://doi.org/10.1088/1755-1315/1262/3/032032 DOI: https://doi.org/10.1088/1755-1315/1262/3/032032
Al-Juboory, H. H., & Al-Kubaisei, A. K. (2023). Morphologically and molecularly identification of the fungus that causes white mold disease on eggplant and cucumber. IOP Conference Series: Earth and Environmental Science, 1252(1), 012008. https://doi.org/10.1088/1755-1315/1252/1/012008 DOI: https://doi.org/10.1088/1755-1315/1252/1/012008
AL-Karaawi, K. Z., & Alwaily, D. S. (2022). Isolation and diagnosis of Sclerotinia sclerotiorum (Lib) Debary fungus that causes white mold disease on eggplant plants in Iraq and the effectiveness of some elements of biological control in inhibiting it in vitro. International Journal of Special Education, 37(3), 2865–2879. https://doi.org/10.14704/nq.2022.20.10.NQ55144
Almagro, L., Gomez Ros, L. V., Belchi-Navarro, S., Bru, R., Ros Barcelo, A., & Pedreno, M. A. (2009). Class III peroxidases in plant defence reactions. Journal of Experimental Botany, 60(2), 377–390. https://doi.org/10.1093/jxb/ern277 DOI: https://doi.org/10.1093/jxb/ern277
Al-Shujairi, K. A., Albehadlli, H. K., Kamaluddin, Z. N., Al-Abedy, A. N., & Al-Taey, D. K. (2022). Genetic variation among some Sclerotinia sclerotiorum isolates causing white mold disease in eggplants (Solanum melongena). International Journal of Agricultural and Statistical Sciences, 18(1), 399–407. https://connectjournals.com/03899.2022.18.399
Bai, S., Zhang, M., Tang, S., Li, M., Wu, R., Wan, S., Chen, L., Wei, X., & Li, F. (2024). Research progress on benzimidazole fungicides: A review. Molecules, 29(6), 1218. https://doi.org/10.3390/molecules29061218 DOI: https://doi.org/10.3390/molecules29061218
Freitas, C. D., Costa, J. H., Germano, T. A., Rocha, R. D. O., Ramos, M. V., & Bezerra, L. P. (2024). Class III plant peroxidases: From classification to physiological functions. International Journal of Biological Macromolecules, 263(1), 130306. https://doi.org/10.1016/j.ijbiomac.2024.130306 DOI: https://doi.org/10.1016/j.ijbiomac.2024.130306
Hammerschmidt, R., Nuckles, E. M., & Kuc, J. (1982). Association of enhanced peroxidase activity with induced systemic resistance of cucumber to Colletotrichum lagenarium. Physiological Plant Pathology, 20(1), 73–82. DOI: https://doi.org/10.1016/0048-4059(82)90025-X
Hasan, K. A., Soliman, H., Baka, Z., & Shabana, Y. M. (2020). Efficacy of nano-silicon in the control of chocolate spot disease of Vicia faba L. caused by Botrytis fabae. Egyptian Journal of Basic and Applied Sciences, 7(1), 53–66. https://doi.org/10.1080/2314808X.2020.1727627 DOI: https://doi.org/10.1080/2314808X.2020.1727627
Kovacs, S., Kutasy, E., & Cashbook, J. (2022). The multiple role of silicon nutrition in alleviating environmental stresses in sustainable crop production. Plants, 11(9), 1223.
https://doi.org/10.3390/plants11091223 DOI: https://doi.org/10.3390/plants11091223
Melo, B. S., Voltan, A. R., Arruda, W., Lopes, F. A. C., Georg, R. C., & Ulhoa, C. J. (2019). Morphological and molecular aspects of sclerotial development in the phytopathogenic fungus Sclerotinia sclerotiorum. Microbiological Research, 229, 126326. https://doi.org/10.1016/j.micres.2019.126326 DOI: https://doi.org/10.1016/j.micres.2019.126326
O’Sullivan, C. A., Belt, K., & Thatcher, L. F. (2021). Tackling control of a cosmopolitan phytopathogen: Sclerotinia. Frontiers in Plant Science, 12, 707509. https://doi.org/10.3389/fpls.2021.707509 DOI: https://doi.org/10.3389/fpls.2021.707509
Ordonez-Valencia, C., Ferrera-Cerrato, R., Quintanar-Zuniga, R. E., Flores-Ortiz, C. M., Guzman, G. J. M., Alarcon, A., Larsen, J., & Garcia-Barradas, O. (2015). Morphological development of sclerotia by Sclerotinia sclerotiorum: A view from light and scanning electron microscopy. Annals of Microbiology, 65, 765–770.
https://doi.org/10.1007/s13213-014-0916-x DOI: https://doi.org/10.1007/s13213-014-0916-x
Pandey, V. P., Awasthi, M., Singh, S., Tiwari, S., & Dwivedi, U. N. (2017). A comprehensive review on function and application of plant peroxidases. Biochemistry & Analytical Biochemistry, 6(1), 308. https://doi.org/10.4172/2161-1009.1000308 DOI: https://doi.org/10.4172/2161-1009.1000308
Prasannath, K. (2017). Plant defense-related enzymes against pathogens: A review. AGRIEAST: Journal of Agricultural Sciences, 11(1), 38. https://doi.org/10.4038/agrieast.v11i1.33 DOI: https://doi.org/10.4038/agrieast.v11i1.33
Prova, A., Akanda, A. M., Islam, S., & Hossain, M. M. (2018). Characterization of Sclerotinia sclerotiorum, an emerging fungal pathogen causing blight in hyacinth bean (Lablab purpureus). The Plant Pathology Journal, 34(5), 367–380. https://doi.org/10.5423/PPJ.OA.02.2018.0028 DOI: https://doi.org/10.5423/PPJ.OA.02.2018.0028
Rajeshkumar, S. (2019). Antifungal impact of nanoparticles against different plant pathogenic fungi. In Nanomaterials in plants, algae and microorganisms (pp. 197–217). https://doi.org/10.1016/B978-0-12-811488-9.00010-X DOI: https://doi.org/10.1016/B978-0-12-811488-9.00010-X
Rosa-Martínez, E., Adalid-Martínez, A. M., García-Martínez, M. D., Mangino, G., Raigón, M. D., Plazas, M., Gramazio, P., Prohens, J., & Vilanova, S. (2022). Fruit composition of eggplant lines with introgressions from the wild relative S. incanum: Interest for breeding and safety for consumption. Agronomy, 12(2), 266. https://doi.org/10.3390/agronomy12020266 DOI: https://doi.org/10.3390/agronomy12020266
Salih, Y. A., & Al-Mansoury, B. A. R. A. H. (2021). Evaluation of the efficiency of some bioagents and their interaction with the fungicide Topsin-M in the controlling eggplant root rot disease caused by Fusarium oxysporum. International Journal of Agricultural & Statistical Sciences, 17, 2153–2162. https://connectjournals.com/03899.2021.17.2153
Sharma, M., & Kaushik, P. (2021). Biochemical composition of eggplant fruits: A review. Applied Sciences, 11(15), 7078. https://doi.org/10.3390/app11157078 DOI: https://doi.org/10.3390/app11157078
Smolinska, U., & Kowalska, B. (2018). Biological control of the soil-borne fungal pathogen Sclerotinia sclerotiorum: A review. Journal of Plant Pathology, 100, 1–12. https://doi.org/10.1007/s42161-018-0023-0 DOI: https://doi.org/10.1007/s42161-018-0023-0
Wang, L., Ning, C., Pan, T., & Cai, K. (2022). Role of silica nanoparticles in abiotic and biotic stress tolerance in plants: A review. International Journal of Molecular Sciences, 23(4), 1947. https://doi.org/10.3390/ijms23041947 DOI: https://doi.org/10.3390/ijms23041947
Wilson, G. W. T., & Williamson, M. M. (2008). Topsin-M: The new benomyl for mycorrhizal-suppression experiments. Mycologia, 100(4), 548–554. https://www.researchgate.net/publication/23298313 DOI: https://doi.org/10.3852/08-024R
Zanatta, T. P., Kulczynski, S. M., Guterres, C. W., Fontana, D. C., Meira, D., Ceolin, E. L., Balem, E., Trevisan, M., Paraginski, J. A., & Buffon, P. A. (2019). Morphological and pathogenic characterization of Sclerotinia sclerotiorum. Journal of Agricultural Science, 11(8), 302. https://doi.org/10.5539/jas.v11n8p302 DOI: https://doi.org/10.5539/jas.v11n8p302
Zhang, Q., Wang, J., Liu, M., Ma, X., Bai, Y., Chen, Q., Sheng, S., & Wang, F. (2023). Nano-silicon triggers rapid transcriptomic reprogramming and biochemical defenses in Brassica napus challenged with Sclerotinia sclerotiorum. Journal of Fungi, 9(11), 1108. https://doi.org/10.3390/jof9111108 DOI: https://doi.org/10.3390/jof9111108
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