BIOLOGICAL ACTIVITY OF PURIFIED MANNOPROTEIN FROM BAKER’S YEAST AGAINST PSEUDOMONAS AERUGINOSA
DOI:
https://doi.org/10.36103/50htkd15Keywords:
amino acids,, antibiofilm activity, , FE-SEM,, FTIR,, mannoproteins.Abstract
This study aimed to evaluate the biological activity of mannoprotein purified from baker's yeast Saccharomyces cerevisiae available in local markets. The mannoprotein was extracted, purified and the emulsifying activity was detected at each step. The emulsifying activity of crude mannoprotein was 67.85%. The crude mannoprotein was partially purified by precipitation using cold ethanol and the emulsifying efficiency reached to 75%. Then it was completely purified using gel filtration chromatography with Sephadex G-100 gel, the purified mannoprotein had 37.61 mg/ml protein and 32.93 mg/ml carbohydrate with emulsifying activity 87.55%. Mannoprotein was characterized using FTIR and amino acid analysis which showed that the mannoprotein was glycoprotein. The antibacterial and antibiofilm activity of mannoprotein against Pseudomonas aeruginosa isolates from different clinical sources was tested and show very high effect in the reduction of bacterial growth with rate reached to 98.4%. Also, the mannoprotein inhibited the biofilm formation for bacterial isolates at different rates, the highest rate was 60.09%, while the rates of biofilm degradation were lower and reached to 21.12%. FE-SEM examination showed a reduction in biofilm formation.
Received: 22/10/2024
Accepted: 26/2/2025
Published: 30/5/2026
References
Alcantara, V. A., Pajares, I. G., Simbahan, J. F., Villarante, N. R. & Rubio, M. L. D. (2010). Characterization of biosurfactant from Saccharomyces cerevisiae 2031 and evaluation of emulsification activity for potential application in bioremediation. Philippine. Agric. Sci. 93(1), 22-30.
Assunção Bicca, S., Poncet-Legrand, C., Williams, P., Mekoue Nguela, J., Doco, T., & Vernhet, A. (2022). Structural characteristics of Saccharomyces cerevisiae mannoproteins: Impact of their polysaccharide part. Carbohydrate Polymers, 277, 118758. https://doi.org/10.1016/j.carbpol.2021.118758
Bakir, G., Dahms, T. E. S., Martin-Yken, H., Bechtel, H. A., & Gough, K. M. (2024). Saccharomyces cerevisiae cell wall remodeling in the absence of Knr4 and Kre6 revealed by Nano-Fourier Transform Infrared Spectroscopy. Applied Spectroscopy. 78(4), 355–364. https://doi.org/10.1177/00037028231213658
Barth, A. (2000). The infrared absorption of amino acid side chains. Progress in Biophysics and Molecular Biology. 74(3-5), 141–173. https://doi.org/10.1016/s0079-6107(00)00021-3
Bonner, P.L.R. (2019). Protein Purification. 2th ed. pp: 249-287. Taylor and Francis Group. London.
Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254.
Bzducha Wróbel, A., Farkaš, P., Chraniuk, P., Popielarz, D., Synowiec, A., Pobiega, K., & Janowicz, M. (2022). Antimicrobial and prebiotic activity of mannoproteins isolated from conventional and nonconventional yeast species-the study on selected microorganisms. World Journal of Microbiology & Biotechnology. 38(12), 256. https://doi.org/10.1007/s11274-022-03448-5
Chopra, L., Singh, G., Kumar Jena, K., & Sahoo, D. K. (2015). Sonorensin: A new bacteriocin with potential of an anti-biofilm agent and a food biopreservative. Scientific Reports. 5,13412. https://doi.org/10.1038/srep13412
De Meutter, J. & Goormaghtigh, E. (2021). Amino acid side chain contribution to protein FTIR spectra: impact on secondary structure evaluation. European Biophysics Journal : EBJ. 50(3-4), 641–651.
Dhivya, H., Balaji, S., & Madhan Rand Srv, A. (2014). Production of amphiphilic surfactant molecule from Saccharomyces cerevisiae Mtcc 181 and its protagonist in nanovesicle synthesis. Int. J. Pharma Sci. 3(11),16-23.
Drozdova, P., Gurkov, A., Saranchina, A., Vlasevskaya, A., Zolotovskaya, E., Indosova, E., Timofeyev, M., & Borvinskaya, E. (2024). Transcriptional response of Saccharomyces cerevisiae to lactic acid enantiomers. Applied Microbiology and Biotechnology. 108(1), 121. https://doi.org/10.1007/s00253-023-12863-z
Dubois, N., Gilles, K. A., Hamilton, J. K., Rebers, P. A., & Smith, F. (1956). Colorimetric method for detection of sugars and related substances. Anal. Chem. 28, 350-356.
Elsaygh, Y. A., Gouda, M. K., Elbahloul, Y., Hakim, M. A., & El Halfawy, N. M. (2023). Production and structural characterization of eco-friendly bioemulsifier SC04 from Saccharomyces cerevisiae strain MYN04 with potential applications. Microbial Cell Factories. 22(1), 176. https://doi.org/10.1186/s12934-023-02186-z
Ghanegolmohammadi, F., Okada, H., Liu, Y., Itto-Nakama, K., Ohnuki, S., Savchenko, A., Bi, E., Yoshida, S. & Ohya, Y. (2021). Defining functions of mannoproteins in Saccharomyces cerevisiae by high-dimensional morphological phenotyping.
Journal of Fungi (Basel, Switzerland). 7(9), 769. https://doi.org/10.3390/jof7090769
Ghosh, S., Upadhay, A., Singh, A., & Kumar, A. (2010). Investigation of antimicrobial activity of silver nano particle loaded cotton fabrics which may promote wound healing. International Journal of Pharma and Bio Sciences. 1(3),1-10.
Giovani, G., Canuti, V. & Rosi, I. (2010). Effect of yeast strain and fermentation conditions on the release of cell wall polysaccharides. Int. J. Food Microbiol. 137(2-3), 303-307.
Gonzalez-Ramos, D. & Gonzalez, R. (2006). Genetic determinants of the release of mannoproteins of enological interest by Saccharomyces cerevisiae. J. Agric. Food. Chem. 54(25), 9411-9416.
Hutti-Kaul, R. & Mattiasson, B. (2003). Isolation and Purification of Proteins. 6th ed. pp: 515-548. Marcel Dekker. New York.
Ibrahim, A. H. (2022). Link between some virulnce factors genes and antibacterial resistance of Pseudomonas aeruginosa. Iraqi Journal of Agricultural Sciences. 53(5), 985-993.
https://doi.org/10.36103/ijas.v53i5.1612
Idrees, M., Mohammad, A. R., Karodia, N., & Rahman, A. (2020). Multimodal role of amino acids in microbial control and drug development. Antibiotics (Basel, Switzerland). 9(6), 330. https://doi.org/10.3390/antibiotics9060330
Jaffar, N., Ishikawa, Y., Mizuno, K., Okinaga, T., & Maeda, T. (2016). Mature biofilm degradation by potential probiotics: aggregatibacter actinomycetemcomitans versus Lactobacillus spp. Plos One. 11(7), e0159466. https://doi.org/10.1371/journal.pone.0159466
Kadhem, B.Q., Essa, R. H., & Mahmood, N. N. (2019). Antimicrobial activity of a bioemulsifiеr produced by Saccharomyces cerevisiae. J. Univ. Garmian. 6 (1),546- 554.
Karaca, B., Haliscelik, O., Gursoy, M., Kiran, F., Loimaranta, V., Söderling, E., & Gursoy, U. (2022). Analysis of chemical structure and antibiofilm properties of exopolysaccharides from Lactiplantibacillus plantarum EIR/IF-1 postbiotics. Microorganisms. 10. 2200. 10.3390/microorganisms10112200.
Le, N. M. T., So, K. K., Chun, J., & Kim, D. H. (2024). Expression of virus-like particles (VLPs) of foot-and-mouth disease virus (FMDV) using Saccharomyces cerevisiae. Applied Microbiology and Biotechnology. 108(1), 81. https://doi.org/10.1007/s00253-023-12902-9
Liu, H. Z., Liu, L., Hui, H., & Wang, Q. (2014). Structural characterization and antineoplastic activity of Saccharomyces cerevisiae mannoprotein. International Journal of Food Properties. 18(2), 359–371. https://doi.org/10.1080/10942912.2013.819364
Magengelele, M., Malgas, S. & Pletschke, B. (2023). Bioconversion of spent coffee grounds to prebiotic mannooligosaccharides – an example of biocatalysis in biorefinery. RSC Advances. 13, 3773-3780.
Mnif, I. & Ghribi, D. (2015). High molecular weight bioemulsifiers, main properties and potential environmental and biomedical applications. World J. Microbiol. Biotechnol. 31(5), 691-706.
Mujumdar, S., Bhandari, T. & Atre, C. (2014). Industrial and biological applications of bioemulsifIers: A mini review. J. Biomed and Pharm. Res, 4 (4), 26-35.
Novačić, A., Vučenović, I., Primig, M., & Stuparević, I. (2020). Non-coding RNAs as cell wall regulators in Saccharomyces cerevisiae. Critical Reviews in Microbiology. 46(1), 15–25. https://doi.org/10.1080/1040841X.2020.1715340
Pérez-Través, L., Lopes, C. A., González, R., Barrio, E., & Querol, A. (2015). Physiological and genomic characterisation of Saccharomyces cerevisiae hybrids with improved fermentation performance and mannoprotein release capacity. International Journal of Food Microbiology. 205, 30–40. https://doi.org/10.1016/j.ijfoodmicro.2015.04.004
Rasheed, H. GH. & Haydar, N. H. (2023). Purification, characterization and evaluation of biological activity of mannoprotein produced from Saccharomyces cerevisiae by. Iraqi Journal of Agricultural Sciences. 54(2), 347-359. https://doi.org/10.36103/ijas.v54i2.1709
Saleh, A. Y. A. (2021). Effect of Mannoprotein Extracted from Saccharomyces Cerevisiae Yeast to Some Pathogenic Bacteria. M.Sc. Thesis, College of Basic Education - Al-Mustansiriya University.
Salman, J. A.S. & Kareem, A. J. (2021). Antibacterial and anti virulence factors of purified dextran from Lactobacillus gasseri against Pseudomonas aeruginosa. Jordan Journal of Biological Sciences. 14(1), 191-197.
Salman, J. A.S. (2013). Antibacterial activity of silver nanoparticles synthesized by Lactobacillus spp. against Methicillin Resistant Staphylococcus aureus. International Journal of Advanced Research. 1, 178-184.
Salman, Z. J., Salman, J. A.S., & Aziz, R. A. (2024). Determination of multi drug resistance (MDR) Pseudomonas aeruginosa isolated from clinical sources. Journal of the College of Basic Education. 1(Special Iss.), 92-108.
Snyman, C., Mekoue Nguela, J., Sieczkowski, N., Divol, B., & Marangon, M. (2023). Characterization of mannoprotein structural diversity in wine yeast species. Journal of Agricultural and Food Chemistry. 71(49), 19727–19738. https://doi.org/10.1021/acs.jafc.3c05742
Su, L., Feng, Y., Wei, K., Xu, X., Liu, R., & Chen, G. (2021). Carbohydrate-based macromolecular biomaterials. Chemical reviews. 121(18), 10950–11029. https://doi.org/10.1021/acs.chemrev.0c01338
Walencka E, Wieckowska-Szakiel, M., Rozalska, S., Sadowska, B., & Rozalska, B. (2007). A surface-active agent from Saccharomyces cerevisiae influences staphylococcal adhesion and biofilm development. Zeitschrift Fur Naturforschung C. 62(5–6),433–438.
https:// doi.org/ 10. 1515/ znc- 2007-5- 618
Wang, Y., Haqmal, M. A., Yue-dong Liang, Y. D., Muhammad, I., Zhao, X. O., Elken, E. M., Gao, Y. H., Jia, Y. U., He, C. G., Wang, Y. M., Kong, L. C., & Ma H. X. (2022). Antibacterial activity and cytotoxicity of a novel bacteriocin isolated from Pseudomonas sp. strain 166. Microbial Biotechnology. 15 (9), 2337–2350.
Warraich A.A., Mohammed, A.U.R., Gibson, H., Hussain, M., & Rahman, AS. (2021). Acidic amino acids as counterions of ciprofloxacin: Effect on growth and pigment production in Staphylococcus aureus NCTC 8325 and Pseudomonas aeruginosa PAO1. Plos One. 16(4), e0250705. https://doi.org/10.1371/journal.pone.0250705
Warraich, A. A., Mohammed, A. R., Perrie, Y., Hussain, M., Gibson, H., & Rahman, A. (2020). Evaluation of anti-biofilm activity of acidic amino acids and synergy with ciprofloxacin on Staphylococcus aureus biofilms. Scientific Reports. 10(1), Article 9021. https://doi.org/10.1038/s41598-020-66082-x
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