IN VITRO GAS PRODUCTION OF SOME ROUGHAGES USING DIFFERENT LEVELS OF AWASSI LAMBS AND BUFFALOES RUMEN FLUID
DOI:
https://doi.org/10.36103/ey8e7r69Keywords:
methane gas, metabolizable energy, net energy for milk production, wild reedsAbstract
This study was conducted at the College of Agricultural Engineering Sciences/university of Baghdad. Were used wild reeds and barley straw inoculation with cumulative levels of rumen fluid, Which aimed to calculate in vitro total and methane gas production using five treatments, as follows:-
T1: 1 ml of buffalo rumen fluid + 9 ml of lamb rumen fluid.
T2: 2 ml of buffalo rumen fluid + 8 ml of lamb rumen fluid.
T3: 4 ml of buffalo rumen fluid + 6 ml of lamb rumen fluid.
T4: 6 ml of buffalo rumen fluid + 4 ml of lamb rumen fluid.
T5: 8 ml of buffalo rumen fluid + 2 ml of lamb rumen fluid.
The results showed a highly significant decreases (P≤0.01) in total gas and methane production for the treatments T1, T2, and T3 after 24, 48, and 72 hr. of incubation periods compared with, T4 and T5 for wild reed and barley straw samples. The results also showed a highly significant decreases (P≤0.01) in the production of total gas and methane production after, 48 hr. of incubation periods of barley straw samples compared with wild reeds. The results also showed a highly significant increases (P≤0.01) in the values of the organic matter digestibility, metabolizable energy, and net energy for milk production for treatments T4 and T5 compared to treatments T1, T2, and T3 after 24 hr. of incubation period. Conclude be that addition buffalo rumen fluid didn't decrease of total gas and methane production in in vitro, so this means that buffalo are less efficient than sheep in utilizing feed energy.
Received: 2/8/2023
Accepted: 6/12/2023
Published: 30/5/2026
References
AL-Bayati, M., & Hassan, A. (2018). Effects of in vitro supplementation of mulberry leaf flavonoids on microbial flora, methanogenesis and fermentative products in rumen fluid of sheep. Journal of Research in Ecology, 6, 2067–2077.
Alemu, A. W., Ominski, K. H., & Kebreab, E. (2011). Estimation of enteric methane emissions trends (1990–2008) from Manitoba beef cattle using empirical and mechanistic models. Canadian Journal of Animal Science, 91(2), 305–321. https://doi.org/10.4141/cjas2010-009
Al-Nadawi, M. F. M., & Al-Samarrai, W. H. (2024). Inoculation of the rumen fluid of Awassi lambs with the rumen fluid of Iraqi buffalo and its effect on the in vitro digestibility coefficient of the dry and organic matter of barley straw and some characteristics of the rumen fluid. Journal of Global Innovations in Agricultural Sciences, 12(1). [In press].
Al-Nadawi, M. F. M., & Al-Samarrai, W. H. (2024). Impact of inoculation of rumen fluid of Awassi lambs with ruminal fluid of Iraqi buffalo on in vitro digestion of Iraqi wild reeds and some ruminal fluid characteristics. Journal of Global Innovations in Agricultural Sciences, 12(1), 1–6. https://doi.org/10.22194/JGIAS/24.1190
Al-Samaraee, W. H., Ahmed, A. A., Hussein, H. Z., & Alwaeli, S. N. (2019). Effect of Trichoderma harzianum on chemical composition and in vitro digestibility of crop residues. Plant Archives, 19(2), 3623–3628.
Alwaeli, S., Al-Samaraae, W., & Al-Saady, Y. (2017). Effect of barley straw treatment with probiotic on some productive characteristics. Research Journal of Pharmaceutical, Biological and Chemical Sciences, 8(6), 308–314.
Belanche, A., de la Fuente, G., & Newbold, C. J. (2015). Effect of progressive inoculation of fauna-free sheep with Holotrich protozoa and total-fauna on rumen fermentation, microbial diversity and methane emissions. FEMS Microbiology Ecology, 91(3), 1–10. https://doi.org/10.1093/femsec/fiu026
Benchaar, C., Pomar, C., & Chiquette, J. (2001). Evaluation of dietary strategies to reduce methane production in ruminants: A modelling approach. Canadian Journal of Animal Science, 81(4), 563–574. https://doi.org/10.4141/A00-119
Cao, Y., Takahashi, T., Horiguchi, K. I., Yoshida, N., & Cai, Y. (2010). Methane emissions from sheep fed fermented or non-fermented total mixed ration containing whole-crop rice and rice bran. Animal Feed Science and Technology, 157(1-2), 72–78. https://doi.org/10.1016/j.anifeedsci.2010.02.004
Cao, Y., Cai, Y., Takahashi, T., Yoshida, N., Tohno, M., Uegaki, R., Nonaka, K., & Terada, F. (2011). Effect of lactic acid bacteria inoculant and beet pulp addition on fermentation characteristics and in vitro ruminal digestion of vegetable residue silage. Journal of Dairy Science, 94(8), 3902–3912. https://doi.org/10.3168/jds.2010-3623
Caro, D., Davis, S. J., Bastianoni, S., & Caldeira, K. (2014). Global and regional trends in greenhouse gas emissions from livestock. Climatic Change, 126, 203–216. https://doi.org/10.1007/s10584-014-1197-x
Chang, J., Peng, S., Yin, Y., Ciais, P., Havlik, P., & Herrero, M. (2021). The key role of production efficiency changes in livestock methane emission mitigation. AGU Advances, 2(2), 1–16. https://doi.org/10.1029/2021AV000391
Duncan, D. B. (1955). Multiple range and multiple F tests. Biometrics, 11(1), 1–42.
Fievez, V., Babayemi, O. J., & Demeyer, D. (2005). Estimation of direct and indirect gas production in syringes: A tool to estimate short chain fatty acid production that requires minimal laboratory facilities. Animal Feed Science and Technology, 123, 197–210. https://doi.org/10.1016/j.anifeedsci.2005.05.001
Gerber, P. J., Steinfeld, H., Henderson, B., Mottet, A., Opio, C., Dijkman, J., Falcucci, A., & Tempio, G. (2013). Tackling climate change through livestock: A global assessment of emissions and mitigation opportunities. Food and Agriculture Organization of the United Nations (FAO).
Haque, M. N. (2018). Dietary manipulation: A sustainable way to mitigate methane emissions from ruminants. Journal of Animal Science and Technology, 60(1), 1–10. https://doi.org/10.1186/s40781-018-0175-7
Hassan, A. (2014). Effect of different levels of molasses on nutritive value and in vitro gas production of sesbania (Sesbania sesban) silage. Jordan Journal of Agricultural Sciences, 10(2), 388–397.
Hassan, A. A., & Abd Alah, M. A. (2018). Effect of adding linseed oil in vitro gas and methane production and some fermentation characters. Egyptian Journal of Applied Science, 33(3), 52–66.
Hassan, A. A., & Abd Alah, M. A. (2020). Effect of adding different levels of bisr date powder in total gas and methane production and in vitro digestibility. Plant Archives, 20(1), 907–914.
Hassan, A. A., & Irhaeem, M. M. (2016). Effect of sun flower or yellow corn oils supplementation to the diet on in vitro gas production. Egyptian Journal of Sheep and Goats Sciences, 11(3), 1–8.
Hassan, A. A., & Al-Samaraee, W. H. (2020). Improvement nutritive value of oyster mushrooms (Pleurotus pulmonarius) residues silage by urea or poultry litter supplementation. Biochemical and Cellular Archives, 20(2), 4957–4961. https://connectjournals.com/03896.2020.20.4957
Hassan, A. A., & Alsamraee, W. H. (2015). Effect of probiotic and urea on nutritive value of malva and barley silage. Proceedings of the 2nd International Conference on Sustainable Agriculture and Environment (2nd ICSAE), 405–410.
Hegarty, R. S., Alcock, D., Robinson, D. L., Goopy, J. P., & Vercoe, P. E. (2010). Nutritional and flock management options to reduce methane output and methane per unit product from sheep enterprises. Animal Production Science, 50(12), 1026–1033. https://doi.org/10.1071/AN10104
Hindrichsen, I. K., Wettstein, H. R., Machmüller, A., & Kreuzer, M. (2006). Methane emission, nutrient degradation and nitrogen turnover in dairy cows and their slurry at different milk production scenarios with and without concentrate supplementation. Agriculture, Ecosystems & Environment, 113(1-4), 150–161. https://doi.org/10.1016/j.agee.2005.09.004
Hussein, D. A., & Hassan, A. A. (2019). Effect of addition of urea to the barley straw for production of gases in vitro. Al-Anbar Journal of Veterinary Sciences, 12(2), 30–37. https://doi.org/10.37940/AJVS.2019.12.2.4
Islam, M., & Lee, S. S. (2019). Advanced estimation and mitigation strategies: A cumulative approach to enteric methane abatement from ruminants. Journal of Animal Science and Technology, 61(3), 122–137. https://doi.org/10.5187/jast.2019.61.3.122
Johnson, K. A., & Johnson, D. E. (1995). Methane emissions from cattle. Journal of Animal Science, 73(8), 2483–2492. https://doi.org/10.2527/1995.7382483x
Kadhim Al-ghazali, B. N., & Muhammad, S. F. (2022). Effect of using different levels of nano-zinc oxide with date by products on in vitro digestibility, fermentation characteristics and gas production. Journal of Karbala for Agricultural Sciences, 9(2), 12–21. https://www.iasj.net/iasj/download/8c8684ee46f2c5b2
Keady, T. W. J., Marley, C. M., Scollan, N. D., Kuoppala, K., Rinne, M., & Vanhatalo, A. (2012). Grass and alternative forage silages for beef cattle and sheep: Effects on animal performance. Proceedings of the XVI International Silage Conference, 2(4), 152–165.
Kim, E. J., Newbold, C. J., & Scollan, N. D. (2011). Effect of water-soluble carbohydrate in fresh forage on growth and methane production by growing lambs. Advances in Animal Biosciences, 2(2), 270–278.
Króliczewska, B., Pecka-Kiełb, E., & Bujok, J. (2023). Strategies used to reduce methane emissions from ruminants: Controversies and issues. Agriculture, 13(3), 602. https://doi.org/10.3390/agriculture13030602
Külling, D. R., Dohme, F., Menzi, H., Sutter, F., Lischer, P., & Kreuzer, M. (2002). Methane emissions of differently fed dairy cows and corresponding methane and nitrogen emissions from their manure during storage. Environmental Monitoring and Assessment, 79, 129–150. https://doi.org/10.1023/A:1020248700255
Kuttar, A. H., Ali, A. A., & Rashid, M. H. (2017). Effect of adding castor oil in the production of total gas and methane production and some fermentation characteristics in in vitro. Al-Anbar Journal of Veterinary Sciences, 10(2), 165–172.
Kuttar, A. H., Majid, H. R., & Hassan, A. A. (2018). Effect of adding linseed oil on in vitro gas and methane production and some fermentation characteristics. European Academic Research, 6(1), 59–74.
Martin, C., Morgavi, D. P., & Doreau, M. (2010). Methane mitigation in ruminants: From microbe to the farm scale. Animal, 4(3), 351–365. https://doi.org/10.1017/S1751731109990620
McDermott, K., Lee, M. R., McDowall, K. J., & Greathead, H. M. (2020). Cross inoculation of rumen fluid to improve dry matter disappearance and its effect on bacterial composition using an in vitro batch culture model. Frontiers in Microbiology, 11, 1–18. https://doi.org/10.3389/fmicb.2020.531404
Menke, K. H., Raab, L., Salewski, A., Steingass, H., Fritz, D., & Schneider, W. (1979). The estimation of the digestibility and metabolizable energy content of ruminant feeding stuffs from the gas production when they are incubated with rumen liquor in vitro. The Journal of Agricultural Science, 93(1), 217–222.
Menke, K. H. (1988). Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Animal Research and Development, 28, 7–55.
Milich, L. (1999). The role of methane in global warming: Where might mitigation strategies be focused? Global Environmental Change, 9(3), 179–201.
Mohammad, S. F., & Alsahaf, E. F. H. (2022). Addition of garlic powder to the ration mixture and its effect on digestion coefficient of dry and organic matter, total gas and methane in vitro production. International Journal of Health Sciences, 6(S4), 5908–5914. https://doi.org/10.53730/ijhs.v6nS4.9467
Morgavi, D. P., Forano, E., Martin, C., & Newbold, C. J. (2010). Microbial ecosystem and methanogenesis in ruminants. Animal, 4(7), 1024–1036.
Moss, A. R., Givens, D. I., & Garnworthy, P. C. (1995). The effect of supplementing grass silage with barley on digestibility, in sacco degradability, rumen fermentation and methane production in sheep at two levels of intake. Animal Feed Science and Technology, 55(1-2), 9–33.
https://doi.org/10.1016/0377-8401(95)00799-S
Patra, A. K. (2012). Estimation of methane and nitrous oxide emissions from Indian livestock. Journal of Environmental Monitoring, 14(10), 2673–2684. http://pubs.rsc.org/doi:10.1039/C2EM30396E
SAS Institute. (2012). SAS user’s guide: Statistics. SAS Institute Inc.
Shibata, M., & Terada, F. (2010). Factors affecting methane production and mitigation in ruminants. Animal Science Journal, 81(1), 2–10.
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