COMPARATIVE EVALUATION OF SYNTHETIC OVAPRIM AND NATURAL CARP PITUITARY EXTRACT FOR INDUCED SPAWNING IN CYPRINUS CARPIO IN SYRIA
DOI:
https://doi.org/10.36103/jqnpzv28Keywords:
CPE, Fecundity, Hatching rate, Latency period, Ovulation rateAbstract
This study compared the effectiveness of synthetic hormone Ovaprim and natural Carp pituitary extract (CPE) in inducing spawning in common Carp. A total of 12 females and 24 males were divided into two groups. The first group received a single dose of Ovaprim (0.5 ml/kg for females, 0.25 ml/kg for males). The second group received CPE: females were given two injections (10% + 90%, total 4 mg/kg), and males one dose (2 mg/kg). Fertilization was done by dry method 8–10 hours after injection. Hatching occurred after 46 hours in Ovaprim group and 42 hours in CPE group. Statistical analysis showed significantly better results for Ovaprim in ovulation rate (100% vs. 83.33%), fertilization rate (96.5% vs. 76%), and hatching rate (97% vs. 63.21%). Latency period was longer with Ovaprim (12h 38m vs. 10h 21m).
Received: 16/4/2025
Accepted: 23/7/2025
Published: 30/5/2026
References
Al-Hilli, A. M. S. 2009. Determination of Enetic and Phenotypic Variation In Common Carp (Cyprinus carpio). Higher Diploma Thesis. Technical College / Al-Musayyab / Technical Education Authority. 58p.
Al-Hilli, S. M. A. & KH. I. Salih. 2013. Use of some sexual inducers for hormonal induction in common carp (Cyprinus carpio) a-Domperidone. Al-Muthanna Journal of Agricultural Sciences. 1(2): 12.
Asal, F. Z. & Kh. I. Saleh. 2015. Use of some ovulation stimulating substances in artificial reproduction of common carp (Cyprinus carpio). Al-Furat Journal of Agricultural Sciences. 7(1): 96-104.
Azawy, M. S. & A. A-Al. Issa. 2019. Comparison of Some Somatic and Reproductive Characters for Tow Brood Stock of (Cyprinus Carpio). Journal of University of Babylon, Pure and Applied Sciences. 27(1): 10.
Beckman, W. C. 1962. The Freshwater Fishes of Syria and their General Biology and Management. FAO, Rome. 297p.
Brzuska, E. 2021. Reproduction effectiveness of carp (Cyprinus carpio L.) from Hungarian W breeding line after stimulating ovulation with spawning inducing agents of natural (CPH, hCG, PMSG) and/or synthetic origin (Ovopel, Dagin, Ovaprim, mGnRH-a). 532: 736023. https://doi.org/10.1016/j.aquaculture.2020.736023
Brzuska, E. and H. Białowąs. 2002. Artificial spawning of carp, Cyprinus carpio (L.). Aquaculture Research. 33(10): 753-765. https://doi.org/10.1046/j.1365- 2109.2002.00714.x
Çiçek, E., L. Jawad, S. Eagderi, H. R. Esmaeili, A. Mouludi-Saleh, S. Sungur. & R. Fricke. 2023. Freshwater fishes of Iraq: a revised and updated annotated checklist-2023. Zootaxa. 5357(1): 1-49. https://doi.org/10.11646/zootaxa.5357.1.1
Çiçek, E., R. Fricke, S. Eagderi. & S. Sungur. 2022. A review of the alien fishes of Turkish inland waters. Turkish Journal of Zoology. 46(1): 1-13. https://doi.org/10.3906/zoo-2109-13
Costello, C., L. Cao, S. Gelcich, M. A. Cisneros-Mata, C. M. Free, H. E. Froehlich, C. D. Golden, G. Ishimura, J. Maier. & I. Macadam-Somer. 2020. The future of food from the sea. Nature. 588(7836): 95–100.
Dariusz, K. Z., T. Katarzyna, K. K. Maciej, K. Slawomir, J. Marek, K. Luczynski, S. Maciej, S. Andrzej, K. Roman, M. Andrzej, G. Piotr. and K. Patrick. 2007. Spawning Agents and their Application. 403p.
Fahad, K. K. & Z. O. Shuhaib. 2021. Cultivation of different densities of common carp (Cyprinus carpio) in floating cages in the Euphrates River in Nasiriyah city. University of ThiQar Journal of agricultural research. 10(2):16-24. https://doi.org/10.54174/utjagr.v10i2.129
FAO. 2024. The State of World Fisheries and Aquaculture 2024. Blue Transformation in Action. Rome. Food and Agriculture Organization of the United Nations.
Garlock, T., F. Asche, J. Anderson, T. Bjørndal, G. Kumar, K. Lorenzen. & R. Tveteras. 2020. A global blue revolution: aquaculture growth across regions, species, and countries. Rev. Fish. Sci. Aquaculture. 28(1): 107–116.
Hamwi, N. and A. Al-Samman. 2017. Fish Farming and Its Diseases. Hama University Publications. 532p.
Hamwi, N., A. A. Ali. & F. Dib. 2025. Use of ovaprim hormone to induce ovulation and hatching in common carp (Cyprinus carpio) farmed in Syria. Latakia University Journal for Research and Scientific Studies - Biological Sciences Series. 47(1): 14.
Hamwi, N., N. Ali Basha, H. Al-Tajer & T. Farah. 2021. The efficacy of clove oil as an alternative anesthetic on common carp (Cyprinus carpio L.). Journal of Hama University. 4(16): 72-83.
Hossain, M. B., M. M. Rahman, M. G. Sarwer, M. Y. Ali, F. Ahamed, S. Rahman, B. Fulanda, M. M. Rahman, B. R. Subba. & M. Y. Hossain. 2013. Comparative study of carp pituitary gland (PG) extract and synthetic hormone ovaprim used in the induced breeding of stinging catfish, Heteropneustes fossilis (Siluriformes: Heteropneustidae). Our Nature. 10(1): 89-95.
https://doi.org/10.3126/on. v10i1.7755
IUCN. 2023. The IUCN Red List of Threatened Species. Version 2022-2. https://www.iucnredlist.org/ (accessed 12 July 2023).
Jelena, S., L. Berzi-Nagy, G. Gyalog, L. Ardo, Z. Vital, N. Plavsa, S. Krstovic, G. L. Fazekas, A. Horvath. & U. Ljubobratovic. 2024. The rank of intensification factors strength in intensive pond production of common carp (Cyprinus carpio L.). Aquaculture. 583: 740584. https://doi.org/10.1016/j.aquaculture.2024.740584
Karnai, L. & I. Szucs. 2018. Outlooks and Perspectives of the Common Carp Production. Roczniki. Annals. 1508-3535. https://doi.org/10.22004/ag.econ.293551
Lukistyowati, I. & R. Putra. 2023. Hematological and physiological characteristics of the common carp (Cyprinus carpio) reared in saline water and fed with fermented herbs Henni Syawal. AACL Bioflux, 2023; 16: 300-306 http://www.bioflux.com.ro/aacl.
More, P. R., R. Y. Bhandare, S. E. Shinde, T. S. Pathan. & D. L. Sonawane. 2010. Comparative study of synthetic hormones ovaprim and carp pituitary extract used in induced breeding of indian major carps. Libyan Agric. Res. Cen. J. Intl. 1(5): 288-295.
Naeem, M., A. Zuberi, A. Salam, M. Ashraf, N. Elahi, M. Ali, A. Ishtiaq, T. Malik, M. J. Khan, M. M. Ayaz, M. J. Iqbal. & B. Ahmad. 2011. Induced spawning, fecundity, fertilization rate and hatching rate of Grass carp (Ctenopharyngodon idella) by using a single intramuscular injection of ovaprim–C at a fish hatchery Faisalabad, Pakistan. Afr, J, Biotechnol. 10(53): 11048-11053. https://doi.org/10.5897/AJB10.1481
Nandeesha, M. C., K. G. R. Rao, R. Javanna, N. S. Parker, J. J. Parker, P. Varokese, H. P. Keshvanath & C. Shety. 1990. Induced spawning of indian major carps through single application of ovaprim C. in The Second Asian Fisheries Forum (Hirano, R, and I. Hanvu Eds.), Asian Fisheries Society, Manila, Philippines. 581–585p.
Paul, K., S. S. Chatterjee, P. Pai, A. Varshney, S. Juikar, V. Prasad. & S. Dasgupta. 2022. Viable smart sensors and their application in data driven agriculture. Computers and Electronics in Agriculture. 198: 107096. https://doi.org/10.1016/j.compag.2022.107096
Shahi, N., S. M. Mallik. & D. Sarma. 2022. Muscle growth in targeted knockout common carp (Cyprinus carpio) mstn gene with low off-target effects. Aquaculture. 547: 737423.
Solomon, S. G., L. O. Tiamiyu, A. Fada. & V. T. Okomoda. 2015. Ovaprim dosage on the spawning performance of cyprinus carpio. Fishery Technology. 52(4): 213 – 217.
Syndel International Inc. 2004. Selected Ovaprim Field Results. Vancover, British Colombia, Canada. 28p.
Weil, C., A. Fostier. & R. Billard. 1986. Induced Spawning (Ovulation and Spermiation) in Carp and Related Species. Aquaculture of Cyprinids, INRA. 2-85340-791-8. ffhal-02858278f. 119-137p.
Woynarovich, E. and A. Woynarovich. 1980. Modified technology for elimination of stickiness of common carp (Cyprinus carpio) eggs. Aquac. Hung. 2: 19–21.
Xue, L., X. Luo, JH. Xing, D. Wang. And DX. Zhang. 2023. Isolation and pathogenicity evaluation of Escherichia coli O157:H7 from common carp, Cyprinus carpio. Microb Pathog. 2023. Jul 15.,182:106250. Epub ahead of print. PMID : 37454944.
Yaron, Z., A. Bogomolnaya, S. Drori, I. Biton, J. Aizen, Z. Kulikovsky. & B. Levavi-Sivan. 2009. Spawning induction in the carp: past experience and future prospects: a Review. I. J. of Aquacult. Bam. 61(1): 5–26. https://doi.org/10.46989/001c.20537
Yaron, Z., B. Sivan, S. Drori. & Z. Kulikovsky. 2002. Spawning induction in cyprinids: hypophyseal and hypothalamic approaches. Bull. VUL RH Vodnany. 38: 181-193.
Yeasmin, S. M., M. A. Rahman, M. Haq, M. M. M. Hossain & M. H. Rahman. 2013. Effects of hormone on ovulation, fecundity, fertilization and hatching of common carp (Cyprinus carpio). IJAFS: ISSN 2308-4715. Int. j. anim. fish. sci. (An online version Journal): 01-07, August 2013, website: www.gurpukur.com or www.gscience.net. 1(1): 8p.
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