EFFECT OF DEFFERENT LEVELS OF TURMERIC SUPPLEMENTATION WITH DIET ON HUMORAL IMMUNE RESPONSE TO NEWCASTLE AND INFECTIOUS BURSAL DISEASE VIRUS AND HISTOPATHOLOGICAL CHANGES OF SOME INTERNAL ORGAN OF BROILER CHICKENS

Abstract

In this study, evaluated the effect of turmeric dietary addition on immune response against the Newcastle disease virus (NDV) and Infectious bursal disease (IBDV). and histopathological changes in some internal  organs was evaluated in 42 day old broiler chicks. ninety-six Ross broiler chicks were reared from 1  to 42 days old and divided randomly into four groups, Feed and drinking water were offered ad libitum.  Group1 (G1) received turmeric (0.2%), while group2 (G2) received turmeric (0.4%), Group3 (G3) received turmeric (0.6 %) and group4 (G4) was the control positive received basal diet . At the end of the trial  five birds from each treatment were slaughtered  for assessment of  histological section, take same of organ (liver , spleen , and bursa of Fabricius) at 42 day.  Results of ELISA test titer revealed differences among various treatment groups at the end of the experiment. mean ELISA test values of G2 revealed a higher significant at (P< 0.05) in antibody titers  in comparsion with  other groups and control. specimen from liver ,spleen and bursa of fabricius were dissected out for histopathological examination and results of microscopic section showed increase in immune response of  G2 chicken : of microscopic sections of G2 histopathological changes in the  liver tissue revaled mamefisted by  focal mononuclear cells aggregation and proliferation of kupffers cells and slight congestion of blood sinusoids  and congestion of portal bloob vessels with few infiltration of inflammatory cells .The intrahepatic bile ductless exhibited dilation and hyperplasia of their epithelial linings with slight per portal fibrosis compared with G1,G3 which showed no clear histopathological changes. The microscopic examination of the spleen  revaled  range from mild (G1 and G3) to moderate hyperplasia of lymphoid follicles in (G2) . histopathological changes in the bursa of  fabricius  were characterized by moderate follicular hypertrophy  especially in G2 as compared with other treated group's and control .