EVALUATION OF EFFICIENCY OF SOME PLANT EXTRACTS AGAINSTPATHOGEN VAIRIMORPHA(NOSEMA) CERANAE INDIGESTIVE OF HONEY BEES
DOI:
https://doi.org/10.36103/30ff9b24Keywords:
Mate Plant, Cinnamon plant, , Bee diseases.Abstract
This study aimed to evaluate the effectiveness of some plant extracts of yerba mate Ilex paraguariensis and Cinnamonum cassia against the pathogen Vairimorpha (Nosema) ceranae in the digestive system of workers of the global honey bee Apis mellifera L. at different concentrations, and showed The results of the laboratory study showed that the alcoholic extract and powders of mate and cinnamon plants at concentrations of 1, 2, and 3% were efficient concentrations and had no side effects on bees. The alcoholic extract of mate at a concentration of 3% was more efficient than the rest of the other concentrations and reached 2070, 1980, 1730, 1390,1080 and 70 x 103 spores / ml respectively with a reduction rate of 97%, The alcoholic cinnamon extract, at a concentration of 3%, was more efficient than the other concentrations, reaching 2600, 2350, 2260, 2190, 1750, 1390, and 1140 x 103 spores/ml respectively with a reduction rate of 56% after 1,3,7,14,21, and 28 days respectively. Yerba mate powder at a concentration of 3% was more efficient than the rest of the other concentrations, which reached 2470, 2240, 2080, 1750, 1460, and 1020 x 103 spores / ml respectively with a reduction rate of 61%, and cinnamon powder at a concentration of 3% reached 2530, 2440, 2280, 2050, 1920, and 1810 x 103 spores/ml respectively with a reduction of 33% after 1,3,7,14,21, and 28 days respectively.
Received: 12/6/2024
Accepted: 23/10/2024
Published: 31/3/2026
References
1.Al-Baghdadi,S.A.and Sadiq,F.H.(2023). .New records of Halictid bees (Hymenoptera: Halictinae) from Iraq.Bulletin Iraq National History Museum ,17(4):679-697.
https://doi.org/10.26842/binhm.2023.17.4.0679
2.Al-Obaydi,W.H. and Shaher,K.W.(2023). Study on the effect of bee strain and insemination method on the hygienic behavior of bee colonies tested using the pricking method. Earth and Enviromental Science ,1252:1-7.
https://10.1088/1088/1755-1315/1252/012017
3. Al-Rawi, K.M.and Abdul –Aziz,M.K.(2000). Design and Analysis of Agricultural Experiments. Dar Books for Printing and Publishing. University of Al Mosul. Ministry of Higher Education and Scientific Research- Iraq. 2nd Edition: pp:488 .
https://doi.lorg/10.1088/1755-1352/1/12017
4.Augul,R.S.(2018).Study on diversity of bees (Hymenoptera: Apoidea) from different regions of Iraq.Bulletin Iraq National History Museum ,15(1):57-75.
https://doi.org/10.26842/binhm.7.2018.15.1.057
5.Awwad,W.H.and Shaher, K.W.(2023) .Study on the effect of bee strain and pollination method on the hygienic behavior of honey bee colonies tested by liquid nitrogen freezing method.Earth and Enviromental Science, 1262:1-6.
https://doi.org/10.1088/1755-1315/1262/3/032028
6.Bernklau,E., Bjostad,L., Hogeboom,A., Carlisle,A. and Arathi,H.S.(2019).Dietary phytoc-hemicals, honey bee longevity and pathogen tolerance. Insects, 10(14):1-12.
https://doi.org/10.3390/insects10010014
7.Cilia,G., Garrido,C.,Bonetto,M., Tesoriero, D. and Nanetti,A.(2020).Effect of Api-Bioxal® and ApiHerb® treatments against Nosema ceranae infection in Apis mellifera investigated by two qPCR methods. Vet. Sci.,7(3):1-12.
http://doi.org/10.3390/vetsci7030125.
8.Dagher,I.J.and Shaher, K.W.(2023). Isolation and identification of Lactobacillus bacteria in the stomach of honey bees and recording a type of bacteria.Eurphrates Journal of Agriculture Science,15(1):335-341.
http://doi.org/10.18502/ijm.v15i3.12905
9.El-Sawalhi,S.,E.,Fayad,G.,Porras, Abou Fayad, A.and Abdel-Massih,R.M.(2021). The antibacterial activity of libanstin from Ilex paraguariensis (Yerba Mate). Fitoterapia, 153 : 104962.
https://doi.org/10.1016/j.fitote.2021.104962
10.Ferreira,L.M., Xavier,M.G.A., Dias,P.R.R. and Vieira,C.G.H.C.(2021).Extracts of cinnamon (Cinnamomum cassia) and mint (Mentha arvensis) for the fungi control of Sclerotinia sclerotiorum. Revista de Agricultura Neotropical, 8 (3):1-6.
https://doi.org/10.32404/rean.v8i3.6161
11.Folly,A.J.,Koch,H.,Farrell,I.W.,Stevenson,P.C. and Brown, M.J.F. (2021).Agri-environment scheme nectar chemistry can suppress the social epidemiology of parasites in an important pollinator.Proc.R. Soc.,288: 1-10.
https://doi.org/10.1098/rspb.2021.0363
12.Hadi,H.A.and Al-Jassany, R.F. (2023). Mole- cular detection of Nosema ceranae and determining the percentage of colony infection and workers in some provinces of
Iraq. Bionatura,8 (1):1-8.
http://doi.org/10.21931/RB/CSS/2023.08.04.63
13.Hamed,A.H.,Dhahir,S.A. and Abid,F.M. (2016).Determination of water soluble vitamins in Iraqi honey bee and compare with others types by high performance liquid chromato-graphy.Baghdad Science Journal, 13(25):447-457.
http://doi.org/10.21123/bsj.13.2.447-457
14.Kowalska,J., Tyburski,J., Matysiak,K., bowska,M.J.k., Łukaszyk J. and Krzyminska, J.(2021). Cinnamon as a useful preventive substance for the care of human and plant health. Molecules, 26:1-13.
http://doi.org/10.3390/molecules26175299
15.Lazar,R.N.,D.,Mot,E.,Alexa, Boldea, M. and Stef, L.(2021).Influence of essential oils on bioproductive indices and health of bee colonies. Scientific Papers. Series D. Animal Science, 5(2):247-253.
http://doi.org/10.1371/journal.pone .0227484
16.Najm,A.D. and Al-Jassany,R.F.(2023).A ch- ecklist of Megachilid bees (Hymenoptera:Megachilidae), with a new record genus from Iraq. Bulletin Iraq National History Museum ,17(4):571-588.
http://doi.org /10.1128/spectrum.00520-23
17.Okaily,R.A.;Mouhamed,A.J.,;Alqeragouly,A.A.,Hamad,B.S.,Kasem,E.M.,Al-Taweel, A. A. and Mahmood,E.A.(2015).Comparativa between populations of honey bee worker Apis melliphera L.for Baghdad and Babylon province by using outline – based geometric morphometric technique. Iraqi Journal of Biotechnology,14(2) : 108-118.
http://doi.org /10.1007/s13592-019-00
18.Pasca,C.,Matei,I.A.,Diaconeasa,Z.,Erler,S. and Dezmirrean,D.S.(2021).Biologically active extracts from different medicinal plants tests as a potential additives against bee pathogens. Antibiotics,10 (8):960-976.
http://doi.org / 10.3390/antibiotics10080960
19.Panek,M.,V.,J.,Boruvka,Nabelkova,K., Sim–unkova,A.,Zeidler, NovAk,D.,Cern,R. and Eticova, K.(2021).Efficacy of caffeine treatenent for wood protection— influence of wood and fungi species.Polymers,13:1-14.
https://doi.org/10.3390/polym13213758
20.Porrini,M.P., Fernanez,N.J., Garrido, P.M., Gende,L.B.,Medici,S.K and Eguaras.M.J. (2011). In vivo evaluation of antiparasitic activity of plant extracts on Nosema ceranae (Microsporidia) .Apidologie,42:700–707.
https://doi.org/ 10.1021/jf902255h
21.Porrini,M.P., Garrido,P.M., Gende,L.B., Ossini,C.R., Hermida,L., Marcangeli, J.A., and Eguaras, M.J.(2017).Oral administration of essential oils and main components: Study on honey bee survival and Nosema ceranae development. Journal of Apicultural Research ,56, (5): 616–624.
https://doi.org/10.1007/s13592-011-0076-y
22.Shahood,G.H., Al-Saffar H.H. and Mize,F.B.H.(2023).Revision of the genus Xylocopa Latreille,1802 (Hymenoptera: Apidae) with a new record of species in Iraq. Bulletin Iraq National History Museum ,17(3):519-530.
https://doi.org/10.26842/binhm.7.2023..0519
23.Tokarev,Y.S., Huang,W.F., Solter,L.F., Malysh ,J.M Becnel,J.J. and Vossbrinck, C.R.(2020). A formal redefinition of the genera Nosema and Vairimorpha (Microsporidia:Nosematid- ae) and reassignment of species based on molecular phylogenetics. J. Invertebr. Pathol. ,169:1-22.
Downloads
Published
Issue
Section
License
Copyright (c) 2026 Mohammed Shakir Manjy, Kamila Ward Shaher

This work is licensed under a Creative Commons Attribution 4.0 International License.

2.jpg)
