FACTORS INVOLVED IN MICROPROPAGATION OF CHRYSANTHEMUM (CHRYSANTHEMUM MORIFOLIUM )
DOI:
https://doi.org/10.36103/fkvbje80Keywords:
BA, Chrysanthemum, Kinetin, Licorice extract, Sucrose, pH and Tissue cultureAbstract
Various concentrations of plant growth regulators added to MS (Murashige & Skoog) media were used to multiply chrysanthemum shoots. pH, sugar and licorice extract. Before being used as attest material, using the ex vitro obtained plant, a nodal piece was sterilized with 1.0% mercuric chloride (HgCl2) for five minutes.MS medium supplemented with BA at a lower concentration (1.0 mg.l-1) showed the best results when compared to other concentrations (8.7 shoots/explants, 7.5 cm, 17.1 leaves/explants and 4.0 nodes/explants)were studied. The average shoot length, nodes per explants, leaves per explants, and number of shoots per explants were all greater in the medium containing 30 g.l-1 sucrose, with values of 6.0 shoots per explants, 4.83 cm, 14.2 leaves per explants, and 3.6 nodes per explants, respectively Results showed that the effect of licorices extract gave the highest response percentage at concentration 50 ml.l-1 (13.5 shoots /explants, 8.5 node/explants, 3.5 leaves / explants and 4.82cm) respectively. It was shown that media with a pH adjustment of 5.7 produced the maximum nodes per shoot, leaves per shoot, average length of shoot, and number of shoots per explants (3.3 nodes per shoot, 14 leaves per shoot, 5.77 cm and 6 shoots per explants, respectively).Concerning the root formation stage, the concentration (0.1 mg.l-1) IBA with 1/4 MS salt strength gave the highest value for all parameters. While, the acclimatization stage showed the culture media supplemented with soil + Peat moss (1:1) gave the highest response percentage 100%. The best treatment was recorded at 1/2 MS salt strength with (2.5 mg.l-1) licorices extract for most characterize.
Received: 15/12/2023
Accepted: 3/3/2024
Published: 28/02/2026
References
Ahmad, T., Abbasi, N. A., Hafiz, I. A., & Ali, A. (2007). Comparison of sucrose and sorbitol as main carbon energy sources in microprogation of peach rootstock GF-677. Pakistan Journal of Botany, 39(4), 1269-1275. https://www.researchgate.net/profile/Ansar-Ali-5/publication/228508728_Comparison_of_sucrose_and_sorbitol_as_main_carbon_energy_sources_in_micropropagation_of_peach_rootstock_GF-677/links/58c637c192851c0ccbfba007/Comparison-of-sucrose-and-sorbitol-as-main-carbon-energy-sources-in-micropropagation-of-peach-rootstock-GF-677.pdf.
Ali, A., Ahmad, T. N., Abbasi A. and Hafiz, I. A. (2009). Effect of different media and growth regulators on in vitro shoot proliferation of olive cultivar ‘Moraiolo’. Pak. J. Bot., 41(2): 783-795. Doi. http://www.pakbs.org.
Al-Mamori, K. H. A. (2009). Micropropagation of Rosu sp. In vivo. Master Thesis, Department of Life Technologies plant, Musayyib technical college.
Amin, M.N., Rahman, M.M. and Manik, M.S. (2003). In vitro clonal propagation of Paederia foetida L: A medicinal plant of Bangladesh, Plant Tissue Cult., 13, 117-123. https://www.baptcb.org/view-details-article/241.
Arora, J. S. (1990). Introductory Ornamental Horticulture. Kalyani Publishers, New Delhi, p. 48. Flowers and Plants Association.
Aziz, R.A and Al-Taweel, S.K. (2019). Effect of plant growth retadants on stevia (Stevia rebaudiana Bertoni) acclimatization produced in vitro. Plant Archives, 19 (1): 1275-1284. https://www.cabidigitallibrary.org/doi/pdf/10.5555/20209904253.
Banno, K., Yoshida, K., Hayashi, S. and Tanabe, K. (2007). In vitro Propagation of Japanese Pear Cultivars. Japanese Society for Hort. Sci., vol. 58 (1): 37-42. https://www.semanticscholar.org/paper/In-Vitro-Propagation-of-Japanese-Pear-Cultivars-Banno-Yoshida/17bada7a66c35559220beaec55b120d2975feba9.
Bhatia, P. and Ashwath, N. (2005). Effect of medium pH on shoot regeneration from cotyledonary explants of tomato, Biotechnol., 4, 7-10. DOI: 10.3923/biotech.2005.7.10.
Chorpa, R. N. and Choi, I. C. (1956). Medicals plants, 2nd ed. USDA. A forest service northern experiment. Georgia, USA. (11) 1-27.
Danial, G.H., Ibrahim, D. A., Barket, S.A. and Khalil, B.M. (2010). Multiple shoots production from shoot tips of fig tree (ficus carica) and callus induction from leaf segments. Int. J.Pure Apple. Sc. Techno., 20(1):117-124.Available online www.ijopaasat.in.doi. https://www.researchgate.net/profile/Diaa-Ibrahim-4/publication/362813925_Multiple_Shoots_Production_from_Shoot_Tips_of_Fig_Tree_Ficus_carica_L_and_Callus_Induction_from_Leaf_Segments/links/6300b7dfe3c7de4c346ee341/Multiple-Shoots-Production-from-Shoot-Tips-of-Fig-Tree-Ficus-carica-L-and-Callus-Induction-from-Leaf-Segments.pdf?origin=publication_detail&_tp=eyJjb250ZXh0Ijp7ImZpcnN0UGFnZSI6InB1YmxpY2F0aW9uIiwicGFnZSI6InB1YmxpY2F0aW9uRG93bmxvYWQiLCJwcmV2aW91c1BhZ2UiOiJwdWJsaWNhdGlvbiJ9fQ&__cf_chl_tk=4Dk3hzGUZXuV0pVgtoaMAARk9KqG.BWv4RgIqhBUfow-1769869372-1.0.1.1-WAUrssCk21BMImV1xDZ2irUJE115M4_Ds2Lf5YvrEYwEbrahim, M. K.H. and Ibrahim, A. I. (2000). Influence of medium solidification and pH value on in vitro propagation of Maranta leuconeura cv. Kerchoviana. Scientia Horticulturae. Volume 86, Issue 3, 3 November, Pages 211-221. https://doi.org/10.1016/S0304-4238(00)00148-5.
George, E. F., Hall, M. A. and De Klerk, G. J. (2008). Plant Propagation by Tissue Culture. Volume 1. The Background, 3rd Edition, Published by Springer, Dordrecht, the Netherlands.
Haider, H. J. A. (2002).The use of some herbs extracts (weeds) to improve the storage ability and tissue culture of potato (Solanum tuberosum L.).PhD. thesis. College of Agriculture, University of Baghdad. Iraq.
Hasan, M.K., Ara, I., Mondal, M.S.A. and Kabir, Y. (2021).Phytochemistry, pharmacological activity,and potential health benefits of Glycyrrhiza glabra. Heliyon 7(6): 1-10. https://doi.org/10.1016/j.heliyon.2021.e07240.
Kajla S., Shashi, K., Anil, K. , Hidayatullah, M. and Manoj, K. S.(2018). Effect of Growth Regulators on in vitro Shoot Multiplication and Plant Regeneration of Rosa hybriad L. from Nodal Explants. International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Special Issue-7 pp. 3804-3811. https://www.researchgate.net/publication/328477494_Effect_of_Growth_Regulators_on_in_vitro_Shoot_Multiplication_and_Plant_Regeneration_of_Rosa_hybriad_L_from_Nodal_Explants/link/5e216c7f92851cafc38aa36d/download?_tp=eyJjb250ZXh0Ijp7ImZpcnN0UGFnZSI6InB1YmxpY2F0aW9uIiwicGFnZSI6InB1YmxpY2F0aW9uIn19.
Karim, M.Z., Amin, M.N., Asad, Z.U., Islam, S., Hassin, F. R and Alam, R. (2003). Rapid multiplication of Chrysanthemum morifolium through in vitro culture. Pak. J. Biol. Sci. 5(11): 1170-1172. DOI: 10.3923/pjbs.2002.1170.1172.
Karim, M.Z., Amin, M.N., Azad, M.A.K., Begum, F., Islam, M.M. and Alam, R. (2002). Effect of different Plant Growth regulators on in vitro Shoot Multiplication of Chrysanthemum morifolium. Online J. Biol. Sci. 3(6): 553-560. DOI: 10.3923/jbs.2003.553.560.
Long, V., Vinh, B., Don, N., Thuy, D. and Nhut, D. (2006). Microponic technology in disease free chrysanthemum production. J Appli Hort. 7. 67-73. DOI:10.37855/jah.2005.v07i02.18. https://www.horticultureresearch.net/jah/2005_7_2_67_71.PDF.
Meloni, A., Oiva, M.A., Ruiz, H.A. and Martinez, C.A. (2001). Contribution of praline and inorganic solutes to osmotic adjustment in cotton under salt stress. J Plant Nutri 24: 599-612. https://doi.org/10.1081/PLN-100104983.
Misal, V.D. and Chavan, A.M.(2025). Effect of Sucrose Concentration on In Vitro Shoot Regeneration in Stevia rebaudiana Bertoni. International Journal of Science and Research (IJSR). Volume 14 Issue 2, February. DOI: https://dx.doi.org/10.21275/SR25222145814.
Mohamad, S. A., Al-Fauri, A., Megdadib, H., Kair, M., Rida, A., Abu-Rommana, S. S. and Al-Ghzawi, A. (2010). In Vitro Multiplication of Chrysanthemum morifolium Ramat and it is Responses to NaCl Induced Salinity. Jordan Journal of Biological Sciences. Volume 3, Number 3, June, doi. ISSN 1995-6673 Pages 101 – 110. https://jjbs.hu.edu.jo/files/v3n3/In%20Vitro%20Multiplication%20of%20Chrysanthemum.pdf.
Dok, D.W. and Mok. M.C. (2001). Cytokinin metabolism and action. Annual Review in Plant physiology. Plant Mol. Biol. 52: 89-118.DOI: 10.1146/annurev.arplant.52.1.89.
Nhut, D.T., Don, N.T., An, T.T.T., Van T.P.T, Vu, N.H., Huyen, P.X. and Khiem, D.V. (2005). Microponic and hydroponic techniques in disease free chrysanthemum (Chrysanthemum sp.) production. J Applied Hort 7: 67-71. https://www.horticultureresearch.net/jah/2005_7_2_67_71.PDF.
Nowak, B., Miczynski, K. and Hudy, L. (2004).Sugar uptake and utilization during adventitious bud differentiation on in vitro leaf explant of Wegierka Zwykla plum (Prunus domestica), Plant Cell, Tissue Organ Cult., 76, 255-260. DOI:10.1023/B:TICU.0000009247.94819.02.
Razdan, M.K., (1993). Introduction and Techniques: An Introduction to Plant Tissue Culture, Oxford and IBH Publishing, New Delhi, Bambey, Calcutta. https://books.google.co.in/books?id=oe_liIY_tVsC&printsec=frontcover#v=onepage&q&f=false.
Sakano, K., (1990). Proton/phosphate stoichiometry in uptake of inorganic phosphate by cultured cells of Catharanthus roseus (L.) G. Don, Plant Physiol., 93, 479-483. https://www.jstor.org/stable/4276536.
SAS, (2001). SAS/ STAT User’s Guide for Personal Computers, Release 6.12, SAS Institute Inc.
Sauvaire, D. and Galgy, R. (1978). Multiplication of vegetative dela Cannal a sourse par bounturage in vitro. CRA Cad D Sci. Se., 287: 446-470.
Sedlak, J. and Paprstein, F. (2003). Influence of growth regulators on in vitro propagation of Pyrus communis cv. Koporecka. Acta Hort., (616): 379-382. https://www.ishs.org/ishs-article/616_57.
Smith, R.H.(2013).Plant Tissue Culture . Techniques and Experiments. 2nd Edition .Academic Press .San Diego, Newyork. PP (53-54). https://scholar.google.com/scholar_lookup?&title=Plant%20Tissue%20Culture%3A%20Techniques%20and%20Experiments&publication_year=2000&author=Smith%2CR.H.
Soliman, F, Glatt, C.E., Bath, K.G., Levita, L., Jones, R.M., Pattwell, S.S, Jing, D., Tottenham, N. and Amso, D. (2010): A genetic variant BDNF polymorphism alters extinction learning in both mouse and human. Science 327: 863–866.
Taha, F. H. (2002) .Some factors affecting the growth and multiplication of rootstock lemon Citrus Jambhiri Lush and Citrus volkameriana pasq in vivo. Master Thesis. College of Agriculture, University of Baghdad, Iraq.
Verma, O.P., Abha-Singh, A. Verma, S.K., Meetu, C. and Shukla, A. K. (2009). Standarization of growth regulators for rapid shoot proliferation in Chrysanthemum morifolium. Asian Journal of Bio-Science, 4(2): 337-339.doi. https://www.researchgate.net/publication/319643852_Standarization_of_growth_regulators_for_rapid_shoot_proliferation_in_chrysanthemum_morifolium.
Yaseen, S. A. (2022). Micorpropagation of Viburnum opulus (roseum) by using Single nodes. Iraqi Journal of Agricultural Sciences 53(6):1388- 1396.
Yildirim, H., Tilkat, E., Onay, A. and Ozen, H. Ç. (2007). In vitro Embryo Culture of Apricot (Pruns armeniaca L.) cv. Hacıhaliloğlu. International J. Sci. & Techn. 2(2): 99-104. https://www.researchgate.net/publication/237240997_In_Vitro_Embryo_Culture_of_Apricot_Prunus_armeniaca_L_cv_Hacohalilolu.
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