ROLE OF FOLIAR APPLICATION WITH PLANT EXTRACTS, AND ADDING HUMIC ACID IN GROWTH OF Calendula officinalis AND ITS CONTENT FROM ZEAXANTHIN AND LYCOPENE

Authors

  • Saja N. Al-Badri Al-Badri
  • Hussein A. Al-Amrani Al-Amrani

DOI:

https://doi.org/10.36103/46v3rt59

Keywords:

antioxidants, Organic farming, plant extracts, plant pigments

Abstract

The research was conducted at Research Station, College of Agricultural Engineering Sciences - University of Baghdad during fall seasons of 2021-2022 and 2022-2023 with the aim of studying the effect of foliar application with Moringa extract, yeast suspention, and adding humic acid on the vegetative and flowering growth of marigold plant and its content of plant pigments. A factorial experiment including three factors in (RCBD) with three replicates . First factor was foliar application with Moringa leaf extract at concentrations 4,6,8 % (M1,M2,M3), as well as yeast suspension at a concentration 5 and 10 g .L-1 (Y1,Y2), in addition to the control treatment (Y0), and Adding humic acid to the soil at concentrations 0 and 5 ml l-1 (H0,H1), respectively. The results showed a significant superiority for the interaction treatment M3Y2H1 in leaf area, plant and flower dry weight, and the content of Lycopene and Zeaxanthin for both seasons. It reached 75.92 dm², 23.81 g plant-1 , 0.520 g flower plant-1 , 22.95 µg g-1, and 47.27 µg g-1, respectively, for the first season, and 68.27 dm², 22.03 g plant-1, 0.477 g flower plant-1, 17.63 µg g-1, and 44.26 µg g-1, respectively, for the second season compared to the control treatment.

References

• Abdalla, M.M. 2013. The potential of Moringa oleifera extract as a biostimulant in enhancing the growth, biochemical and hormonal contents in rocket (Eruca vesicaria subsp. Sativa) plants. International Journal of Plant Physiology and Biochemistry, 5(3): 42-49.

DOI: 10.5897/IJPPB2012.026

• Abdel-Rahman, S.S.A.,and A.A.S. Abdel-Kader, 2020. Response of Fennel (Foeniculum vulgare, Mill) plants to foliar application of moringa leaf extract and benzyl adenine (BA). South African Journal of Botany, 129 : 113-122.

https://doi.org/10.1016/j.sajb.2019.01.037

• Abdul-Hussein, M.A.A., , A.K.H. Al-Tfeely,and S.A. Al-Jubouri, 2010. Effect of growth regulators and media composition on the micropropagation of Nyctaginaceae Bougainvillea Glabra. Kufa Journal of Agricultural Sciences, 2(1) : 100-108. https://www.researchgate.ne

• Al-Asheebi,S.A. , I. A. Al- Gehani , A. M. Kalifa and A. A. Hamad .2020. Effect of Humic Acid on Vegetative and Reproductive Growth of Squash (Cucurbita pepo L.) under Saline Stress Conditions, The Libyan Jo V. (25): No. (1-2): 66 -74

• Al-Asadi, M. H. S., and A. H. J. Al-Khaikani, 2019. Plant Hormones and their Physiological Effects. Baghdad: Daralwarith printing & ISBN: 9789922917108 ,Al-Qasim Alkhadraa University. pp; 332.

• Al-Bayati, M. Y. and A. K. A.-D. Al-Khalifa. 2019. Study of the response of chrysanthemum plants. Calendula officinalis L. to determine the effect of humic and fulvic acids on it under woody canopy conditions. Kirkuk University. Agricultural Engineering Sciences. 10 (1) 55 – 61. Doi:10.58928/KU19.10109

• Al-Dulaimi, A.F., F. F. Jumaa. 2020. Effect of foliar Spray with Yeast Suspension, Licorice Roots Extract, and Amino Quelant-K compound on the chemical content of Black Hamburg Grapes cultivar Berries , Diyala Agricultural Sciences Journal, Vol. 12 No. special Issue ,pp: 546–557

DOI: https://doi.org/10.52951/dasj.20121046

• Al-Qaisi A. A. D., S. H. M. Altai .2023. The Effect of Mycorrhiza Inoculation and Organic Fertilization on some Growth and Flowering Qualities of the Chrysanthemum Plant Calendula officinalis L., 4th International Conference

of Modern Technologies in Agricultural Sciences IOP Conf. Series: Earth and Environmental Science 1262 (2023) 082035 IOP Publishing. doi:10.1088/1755-1315/1262/8/082035.

• Al-Taweel, S.K.,and I.H.A Al-Anbari,. 2019. Moringa oleifera: A Review on the phytochemical screening, proximate analysis, medicinal, nutritional and plant bio stimulants values of its leaves, pods, seeds and roots. Plant Archives, 19(2) : 1612-1622.

• Alwan,N.M., F. J . Kadhim and S. A. Mahdi .2023. Effect of Licorice Organic Media and Disper Bloom Spraying on vegetative growth and Flowering Gerbera Jamesonii L., 4th International Conference of Modern Technologies in Agricultural Sciences IOP Conf. Series: Earth and Environmental Science 1262 : 042040 IOP Publishing .

doi:10.1088/1755-1315/1262/4/042040

• Alwan, N. M. and S. M. Sadiq.2019. Influence of tuberous root soaking in Salicylic Acid and foliar spray of plants with Benzyladenine on growth , flowering and tuberous root production of Ranunculus Asticus , Plant Archives Vol. 19 No. 2, 2019 pp: 4137-4142 ISSN:2581-6063,ISSN:0972-5210.

URL: http://www.plantarchives.org/19-2/4137-4142%20(5226).pdf

• Al-Zubaidi, S.A.K., and H.A.K. Al-Hasnawi, 2021. The effect of foliar feeding with brosol ,and ground application of humic on the growth and flowering of chrysanthemum (Calendula officinalis L.). Fayoum Journal of Agricultural Research and Development, 35(1) : 80-86. Doi: 10.21608/FJARD.2021.188566.

• Cucu, T., K. Huvaere, , M.A. Van Den Bergh, , C. Vinkx, , and J. Van Loco, 2012. A Simple and Fast HPLC Method to Determine Lycopene in Foods. Food Analytical Methods, 5 : 1221–1228. DOI: 10.1007/s12161-011-9354-6.

• De Miccolis, A.R.M., C. Rotolo ,D. Gerin , D. Abate ,S. Pollastro and F. Faretra .2019. Global transcriptome analysis and differentially expressed genes in grapevine after application of the yeast-derived defense inducer cerevisane. Pest Manag Sci. 75(7):2020–2033. doi: 10.1002/ps.5317.

• Gad, N., M.R., Abdel-Moez, and H. Kandil, 2012. Influence of cobalt and mycorrhizae mediated phosphorus on some higher plants growth and yield. Journal of Basic and Applied Sciences Research, 2(11), 11942-11951. www.textroad.com

• Hammad, S.A.R. and O.A.M. Ali.2014. Physiological and biochemical studies on drought tolerance of wheat plants by application of amino acids and yeast extract. Ann Agric Sci. ,59(1):133–145.

doi: 10.1016/j.aoas.2014.06.018.

• Hasan, A. M. .2019. Effect of Foliar Application of Humic Acid And Benzyladenine on Growth And Flowering of Pot Marigold (calendula Officinalis L.), The Journal of Duhok University, Agriculture and Veterinary Science, Vol. 22 No. 1 ,pp: 69-77.

DOI: https://doi.org/10.26682/avuod.2019.22.1.7.

DOI:https://doi.org/10.36103/ijas.v48i4.366.

• Kadhim, N.A. 2019. Pharmacognostical and Phytochemical Studies of (Calendula Officinalis L.) Cultivated In Iraq, Ph.D.dissertation. Baghdad: Mustansiriyah University, College of Pharmacy. doi:10.1007/s13205-016-0526-3 doi:10.5897/IJPPB2012.026.

• Malik, I.,and H.A Almrani,. 2023. Volatile Oil Yield and its Components of Menthol and Limonene of Peppermint Affected by Foliar Spray and Lighting Intensity. In: 4th International Conference of Modern Technologies in Agricultural Sciences. IOP Conf. Series: Earth and Environmental Science 1262.

doi:10.1088/1755-1315/1262/5/052008.

• Nassif, A.H., N.A.A.J. Al-Zubaidi, and R.M. Abd, 2023. The effect of organic and biological fertilization on the yield of black seed (Nigella Sativa L.). Journal of Agricultural, Environmental and Veterinary Sciences, 7(2) : 20-114.

https://doi.org/ 10.26389/ AJSRP.R190123.

• Oosterhuis, D., D. Loka, , E. Kawakami, and W. Pettigrew, 2014. The physiology of potassium in crop production. Advances in Agronomy, 126, 203-234.

https://doi.org/10.1016/B978-0-12-800132-5.00003-1.

• Paul, M.D. 2009. Medicinal Natural Products. Florida: John Wiley & Sons Ltd. Publication, formerly University of Nottingham, UK ,PP:539 .

• Preedy, V.R and V. B. Patel . 2021. Oxidative Stress and Dietary Antioxidants , Second edition, Publisher: Elsevier ,Science Direct ,PP: 257-269.

https://doi.org/10.1016/B978-0-12-819547-5.00028-6 .

• Rafiee, H., H Naghdi,., A. Mehrafarin, , N. Zarinpanjeh, , A. Sekara, ,and E. Zand, 2016. Application of plant biostimulants as new approach to improve the biological responses of medicinal plants- A Critical Review. Journal of Medicinal Plants, 15(59):6-39.

SID. https://sid.ir/paper/698320/en

• Salehi, M., F. Siahvashi and F. Davoodi, 2022. The evaluation of quantitative characteristics and essential oil composition of (Calendula officinalis L.) affected vermicompost and humic acid. Plant Production and Genetics, 3(1), 85-94.

DOI: 10.34785/J020.2022.357

• Salman, A.D., and E.J. Abdul Rasool, 2022. Effect of ozone enrichment and spraying with organic nutrient on nutrient and water use efficiency and fertilizer productivity of broccoli plant cultivated hydroponically with modified NFT technology. Iraqi Journal of Agricultural Sciences, 53(2), 660-668. https://doi.org/10.36103/ijas.v53i3.1576

• Saloom, Y. F., M. H.A. AL- Hasane and S. H.J. AL-Hchami. 2019. Response of Cultivated Cabbage in Plastic Bags for Spraying with Amino Acids, Fertilization with Humic Acid and Its Effect on Some Vegetative and Quality Traits. Plant Archives ,Vol. 19 (Supplement 1): 1234-1240.. e-ISSN:2581-6063 (online), ISSN:0972-5210

• Sardar, H., A. Nisar, , M.A. Anjum, , S. Naz, , S. Ejaz, , S. Ali, and R. Ahmad, 2021. Foliar spray of moringa leaf extract improves growth and concentration of pigment, minerals and stevioside in stevia (Stevia rebaudiana Bertoni). Industrial Crops and Products, 166, 113485.

https://doi.org/10.1016/j.indcrop.2021.113485

• Taih, M.A., F.A. EL-Emary, and M.M. EL-Fawy, 2023. Response of pot marigold plant (Calendula officinalis L.) to spraying with some growth biostimulants. Archives of Agriculture Sciences Journal, 6(2), 26-43.

DOI:https://dx.doi.org/10.21608/aasj. 2023.304063.

• Xu, J., J. Lin, S. Peng, H. Zhao, , Y. Wang, L. Rao, X. Liao, ,and L. Zhao, 2023. Development of an HPLC-PDA Method for the Determination of Capsanthin, Zeaxanthin, Lutein, β-Cryptoxanthin and β-Carotene Simultaneously in Chili Peppers and Products. Molecules, 28(5), 2362. doi:10.3390/molecules28052362.

• Zafar, J., A. Aqeel, F.I. Shah, N. Ehsan, U.F. Gohar, M. A. Moga,., D. Festila, C. Ciurea, M. Irimie, and R. Chicea, 2021. Biochemical and immunological implications of lutein and zeaxanthin. International Journal of Molecular Sciences, 22, 10910. doi.org/10.3390/ijms222010910.

Downloads

Published

2026-01-26

Issue

Section

Articles

How to Cite

Al-Badri , S. N. A.-B., & Al-Amrani , H. A. A.-A. (2026). ROLE OF FOLIAR APPLICATION WITH PLANT EXTRACTS, AND ADDING HUMIC ACID IN GROWTH OF Calendula officinalis AND ITS CONTENT FROM ZEAXANTHIN AND LYCOPENE. IRAQI JOURNAL OF AGRICULTURAL SCIENCES, 57(1), 55-70. https://doi.org/10.36103/46v3rt59