LEPTIN RECEPTOR GENE POLYMORPHISMS AND Its EFFECT ON SOME PRODUCTIVE AND PHYSIOLOGICAL TRAITS IN LOCAL IRAQI CHICKEN

Authors

  • Mohammad A. K. Al Ani
  • Bassam G.M. Al Khatib

DOI:

https://doi.org/10.36103/kb89xk27

Keywords:

PCR, T16297C SNP, Sanger method, HDL, maturity.

Abstract

This study was conducted in poultry farm in college of agricultural engineering Sciences - University of Baghdad - animal production department. In this study 100 laying hens of local Iraqi chickens were used with age 67day old, were placed in individual cages, each cage was numbered from 1 to 100, for the period from 26/10/2021 till 24/7/2022 to detect the different genotypes of LEPR gene, determining the frequency and percentage of the relevant genotypes local Iraqi chickens, , Productive traits were measured from the sexual maturity in to 100 days for each chicken, the blood samples were collected from 100 laying hens at the 38 weeks of age from the brachial vein, the PCR reaction will done using specific primers, Then sanger sequencing technique were done and three genotypes of leptin receptor gene were obtained TT genotype (wild) , TC (heterozygous) and CC genotype( mutant), the C allele frequency were 0.74 had high significant difference (p<0.01) as compared with T allele were 0.26 ,there were significant effects (p<0.05) between the various genotypes on the feed intake showed CC   genotype had the high mean, followed by TC  then TT, in 1,2 and 6 periods.  The CC genotype had high significant effect (p<0.01) and significant effect (p<0.05) on albumin and HDL concentration respectively followed by TC genotype then TT genotype, The CC genotype   affect significantly on maturity age.

References

1. Al-Hassani, A. S.,D. H. Al-Hassani, and I. A. Abdul-Hassan, 2022. Insulin like growth factor (igf-2) gene polymorphisms influences certain biochemical parameters in broiler chickens. Iraqi Journal of Agricultural Sciences, 53(5):1249-1255. https://doi.org/10.36103/ijas.v53i5.1639

2. Al-khatib B.G.M., A.A. Noori and Q.I. Abdul Kareem, 2018. Association of G143831A SNP of growth hormone gene and some productive traits, physiological parameters of laying hens. Research journal of pharmaceutical, Biological and chemical sciences.9(5): 822-828.

3. Ashwell, C.M., S.M. Czerwinski, D.M. Brocht and J.P. McMurtry, 1999. Hormonal regulation of leptin expression in broiler chickens. Am. J. Physiol. 276, R226eR232.

https://doi.org/10.1152/ajpregu.1999.276.1.R226

4. Besbes, B 2009. Genotype evaluation and breeding of poultry for performance under sub-optimal village conditions. World's Poultry Science Journal (2): 260-271.

https://doi.org/10.1017/S0043933909000221

5. Bornelöv, S., E. Seroussi, S. Yosefi, S. Benjamini, S. Miyara, M. Ruzal, Grabherr, N. Rafati, A. M. Molin, K. Pendavis, S. C. Burgess, L. Andersson and M. Friedman-Einat, 2018. Comparative omics and feeding manipulations in chicken indicate a shift of the endocrine role of visceral fat towards reproduction. BMC Genom. 19: 295.

https://doi.org/10.17863/CAM.27601

6. Cassy S., M. Derouet, S. Crochet, S. Dridi, and M. Taouis, 2003. Leptin and insulin down regulate leptin receptor gene expression in chicken-derived leghorn male hepatoma cells, Poultry Science, vol. 82, no. 10: 1573–1579,

https://doi.org/10.1093/ps/82.10.1573

7. Dana N, EH Vander Waaij and JAM Van Arendonk 2011. Genetic and phenotypic parameter estimates for body weights and egg production in Horro Chicken of Ethipia. Tropical Animal Heath Production 43: 21-28. https://doi.org/10.1007/s11250-010-9649-4

8. Denbow, D.M., 2000. Involvement of central gastrin and cholecystokinin in the regulation of food intake in the neonatal chick. J. Appl. Anim. Res. 18, 129e136.

https://doi.org/10.1080/09712119.2000.9706334

9. Duncan DB 1955. Multiple Rang and Multiple F-test. Biometrics 11(1): 4-42.

10. Eidan, S. M., and S. A. Khudhir, 2023. Association between ATP1A1 gene polymorphisms with semen characteristics in Holstein bulls. Iraqi Journal of Agricultural Sciences, 54(2),330.337. https://doi.org/10.36103/ijas.v54i2.1706

11. El Moujahid E M, S. Chen, S. Jin, Y. Lu D. Zhang, C. Ji and N. Yang. 2014. Association of leptin receptor gene polymorphisms with growth and feed efficiency in meat-type chickens. Poultry Science, 93, 1910–1915. https://doi.org/10.3382/ps.2013-03674

12. Friedman-Einat, M., L.A. Cogburn, S. Yosefi, G. Hen, D. Shinder, A. Shirak, and E. Seroussi, 2014. Discovery and characterization of the first genuine avian leptin gene in the rock dove (Columba livia). Endocrinology 155, 3376e3384. https://doi.org/10.1210/en.2014-1273

13. Horev, G., P. Einat, T. Aharoni, Y. Eshdat, and M. Friedman-Einat. 2000. Molecular cloning and properties of the chicken leptin-receptor (CLEPR) gene. Mol. Cell. Endocrinol. 162:95–106. https://doi.org/10.1016/S0303-7207(00)00205-7

14. Huang, G., J. Li, H. Wang, X. Lan, and Y. Wang, 2014. Discovery of a novel functional leptin protein (LEP) in zebra finches: evidence for the existence of an authentic avian leptin gene predominantly expressed in the brain and pituitary. Endocrinology 155, 3385e3396. https://doi.org/10.1210/en.2014-1084

15. Lei M., C. Luo, X. Peng, M. Fang, Q. Nie, D. Zhang, G. Yang and X. Zhang 2007. Polymorphism of growth-correlated gene associated with fatness and muscle fiber traits in chicken’s. journal of poultry science 86: 835-842. https://doi.org/10.1093/ps/86.5.835

16. Lei, M. M., Q. H. Nie, X. Peng, D. X. Zhang, and X. Q. Zhang. 2005. Single nucleotide polymorphisms of the chicken insulin-like factor binding protein 2 gene associated with chicken growth and carcass traits. Poult. Sci. 84:1191–1198. https://doi.org/10.1093/ps/84.8.1191

17. Lei MM, S.Q. Wu, X B Shao, X W Li, Z Chen, S J Ying and Z D. Shi, 2014. Creating leptin-like bio functions by active immunization against chicken leptin receptor in growing chickens. Domestic Animal Endocrinology, 50, 55–64.

https://doi.org/10.1016/j.domaniend.2014.09.001

18. Lei MM, S.Q. Wu, X.W. Li, C.L. Wang, Z Chen and ZD. Shi., 2014 Leptin receptor signaling inhibits ovarian follicle development and egg laying in chicken hens. Reprod Biol Endocrinol.;12:25. https://doi.org/10.1186/1477-7827-12-25

19. Noori AA, Q.I. Abdul Kareem and G.M. Bassam, 2019. Fifth exon and partial off exon six polymer phisms growth hormone receptor (ghr) gene and its association with some productive and physiological traits of laying hens. Biochemical and Cellular Arshives (1): 1291-1296.

https://doi.org/10.35124/bca.2019.19.1.1291.

20. Ohkubo, T., M. Tanaka, and K. Nakashima. 2000. Structure and tissue distribution of chicken leptin receptor (cOb-R) mRNA. Biochim. Biophys. Acta 1491:303–308. https://doi.org/10.1016/S0167-4781(00)00046-4

21. Sas 2012. Statistical Analysis System, User’s Guide. Statistical. Version 9.1th ed. SAS. Institute Incorporated Cary. N.C. USA.

22. Seroussi, E., Y. Cinnamon, S. Yosefi, O.Genin, J.G. Smith, N. Rafati, S. Bornelov, L. Andersson and M. Friedman-Einat, 2015. Identification of the long-sought leptin in chicken and duck: expression pattern of the highly GC-rich avian leptin fits an autocrine/paracrine rather than endocrine function. Endocrinology 157, 737e751. https://doi.org/10.1210/en.2015-1634

23. Zhang, S., Y. Okuhara, M. Iijima, S. Takemi, I. Sakata, H. Kaiya, H. Teraoka and T. Kitazawa, 2020. Identification of pheasant ghrelin and motilin and their actions on contractility of the isolated gastrointestinal tract. Gen. Comp. Endocrinol. 285, 113294.

Downloads

Published

2025-04-24

Issue

Vol. 56 No. 2 (2025)

Section

Articles

License

Copyright (c) 2025 IRAQI JOURNAL OF AGRICULTURAL SCIENCES

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

How to Cite

Ratha, M. A. K. A. A., & Bassam G.M. Al Khatib. (2025). LEPTIN RECEPTOR GENE POLYMORPHISMS AND Its EFFECT ON SOME PRODUCTIVE AND PHYSIOLOGICAL TRAITS IN LOCAL IRAQI CHICKEN. IRAQI JOURNAL OF AGRICULTURAL SCIENCES, 56(2), 736-743. https://doi.org/10.36103/kb89xk27