IMPACT OF FISH WASTE PROTEIN ENZYMETIC HYDROLYSATE ON GROWTH OF SOME OF Lactobacillus sp. AND Bifidobacterium sp.
DOI:
https://doi.org/10.36103/fe8qmt76Keywords:
Alternative media, cyprinus carpio, degree of hydrolysis, Lactic acid bacteria, peptone.Abstract
This study was aimed to investigate the impact of protein enzymatic hydrolysate extracted from waste of common carp (Cyprinus carpio) on some bacterial growth. Two type protein concentrates were prepared from defatted carp fish waste powder, the first at pH 2/4.5 and the second at pH 12/4.5. They were hydrolysed by pepsin enzyme lyophilized powder ≥ 2500 units/mg conc.1.5gm/100gm protein concentrates for (30, 60, 90, and 120) minutes. The obtained hydrolysates characterized in term of color, amino acids, minerals elements, degree of hydrolysis, solubility in addition to bacterial growth promotor and biomass production properties. The whiteness of acidic and basic protein concentrates were (68.62 and 67.09) %, respectively, their content of essential amino acids were (35.73 and 34.5)%, respectively, they contained similar concentrations of mineral elements. The highest degree of hydrolysis for the concentrate was acheived after 120 minutes of enzymatic reaction time, being (50.8 and 52.4)%, their solubilities were increased with increasing enzymatic reaction time, and highest values were recorded at pH 2 and 10. Number of colonies and biomass of Bifidobacterium infantis, Lactobacillus plantarum and Lactobacillus rhamnosus GG was in alternative media higher than commercial medium (MRS) .
References
1. Abbasiliasi, S., T., Joo Shun, A. , Tengku , B., Fatemeh, R., Nagasundara, M., Shuhaimi and B., Arbakariya. 2017. Fermentation factors influencing the production of bacteriocins by lactic acid bacteria: a review . The Royal Society of Chemistry . 7(5): 29395–29420. https://doi.org/10.1039/C6RA24579J
2. Abdulkarem, A.T., A.A., Hasan, H.R., Khudhur and S.M., Abed. 2024 . Overview of opportunistic bacteria. Kerbala Journal of Pharmaceutical Sciences, 1(23), 11 – 21.
3. Al Mamun , M.D. and R.P., Ghani. 2014 . Estimation of mineral content in some selected fishes of Bangladesh. International Conference on Chemical Engineering . 29(7):19–22.
4. AL- Shamary, E.I. , Z.T., Seedra and A.A. , Sheikh.2023.Invesigation of some bacterial species contaminated with some foods in Baghdad regions. IOP Conference Series, Earth and Environmental Sciences.1252(1): 1–11. http://dx.doi.org/10.1088/17551315/1252/1/012163
5. Alahmad, K. , X. , Wenshui , J., Qixing and X., Yanshun . 2022. Effect of the degree of hydrolysis on nutritional, functional, and morphological characteristics of protein hydrolysate produced from Bighead carp (Hypophthalmichthys nobilis) Using Ficin Enzyme.Foods,11(9),1–17. https://doi.org/10.3390/foods11091320
6. Al-ghanimi, G. M. M., and A. M. Alrubeii. 2024. Effect of elastin hedrolyses on the chemical composition and some oxidation indicators in cold-stored ground beef. Iraqi Journal of Agricultural Sciences, 55(2):885-893. https://doi.org/10.36103/wfj0ra89
7. Alhamid, M. A. M. and A. J. AL Mousawi. 2024 . Physicochemical properties of Aloe Vera gel and its utilization in preparing synbiotic fermented milk with Bifidobacterium Lactis BB – 12. Iraqi Journal of Agricultural Sciences, 55(2), 823 – 835. https://doi.org/10.36103/wg1js439
8. Al-Khshali, M.S. 2019. Effect of different ratio of addition of NaCl to the diets on some growth traits in grass carp ctenopharyngodon idella. Plant Archives, 2019, 19: 908–911.
9. Al-Noori, A. A., A. J. Abuelheni, and M.S.Al-Khshali. 2024. Effect of adding natural and nano zinc oxide to the dite on some growth parameters of Cyprinus carpio L. Iraqi Journal of Agricultural Sciences, 55(5):1612-1619. https://doi.org/10.36103/gz903610
10. Al-Lami, A.R., S.B, Abd-Aljalil and H.Y., Fadel. 2015. Isolation and identification of Cronobacter spp. obtained from different food samples in Iraq. World Journal of Experimental Biosciences . 3(2): 69 – 73. https://www.researchgate.net/publication/327744178
11. Andri, C., H., Dyah, D., Tutuk, D., Mohammad, C., Tan and M., Yusuf. 2022. Fish protein concentrate for human consumption: A review of its preparation by solvent extraction methods and potential for food applications . Annals of Agricultural Sciences. 67(1), 42 – 59 .
12. Anggraini, L. , Y., Marlida, j., Wizna and A., Mirzah. 2019. Optimization of nutrient medium for Pediococcus acidilactici DS15 to produce GABA. Journal of World's Poultry Research. 9(3): 139 – 146. http://dx.doi.org/10.36380/jwpr.2019.17
13. Bamgbose,T.,H.I., Atta and R., Anupkumar. 2021. Bacteriocins of lactic acid bacteria and their industrial application . Korean Society for Lactic Acid Bacteria and probiotics. 194(5): 4377 - 4399. http://dx.doi.org/10.1007s1201002203873
14. Barrangou, R., S. J., Lahtinen, F., Ibrahim and A. C., Ouwehand. 2011. Genus Lactobacilli. In: lactic acid bacteria: microbiological and functional aspects (4 ed.). London: CRC Press (Taylor & Francis Group). https://doi.org/10.1201/b11503
15. Berbegal, C., Y., Benavent-Gil, I., Pardo and S., Ferrer. 2015. A novel culture medium for oenococcus oeni malolactic starter production. Food Science and Technology. 64(1):25–31. https://doi.org/10.1016/j.lwt.2015.05.020
16. Chan , P. , P. , Matanjun , R. , Shapawi , C., Budiman and J., Shya Lee. 2019 . Chemical composition of the fillet, fins, bones and viscera of hybrid grouper (Epinephelus lanceolatus, Epinephelus fuscoguttatus) . Journal of Physics,37(3): 1 – 9 . http://dx.doi.org/10.1088/17426596/1358/1/012008
17. Chen , H., J., Niu , T., Qin , Q., Ma , L., Wang and Q., Shu. 2015 . Optimization of the medium for Lactobacillus acidophilus by PlackettBurman and steepest ascent experiment . Acta Sci. Pol. Technol. Aliment. 14(3), 227–232. https://doi.org/10.17306/j.afs.2015.3.24
18. Cheng , I. ; J., Liao , J., Ciou , L., Huang , Y., Chen and C., Hou. 2020 . Characterization of protein hydrolysates from Eel (Anguilla marmorata) and their application in herbal Eel extracts . Catalysts. 10(2): 1 – 12. https://doi.org/10.3390/catal10020205
19. Faisal, S.A., and Al-Khshali, M.S.2023. Study of the Chemical Properties of the Meat of some Fish Imported to Iraq. IOP Conference Series: Earth and Environmental Science, 2023, 1262(7), 072089
20. Fallah , M., S., Bahram and S.R., Javadian. 2015 . Fish peptone development using enzymatic hydrolysis of silver carp by-products as a nitrogen source in Staphylococcus aureus media . Food Sciences and Nutrition, 3(2): 153–157.
21. Hasan, K.S. , T., Al—Hadedee and J.M., Awda. 2019. Isolation, identification and evaluation of lactic acid bacteria as antibacterial activity . Biochemical and Cellular Archives, 19(1): 1 – 12.
22. Jasim, M. and A., Fayyadh. 2018. Isolation and identification of bacteria that produce amylase .Iraq Journal of market research and consumer protection.10(1): 17 – 26. http://DOI%20%3A%2010.35124/bca.2019.19.1.1339
23. Jogeir , T. , A., Albrektsen , B., Hope and A., Aksnes. 2006. Chemical composition, mineral content and amino acid and lipid profiles in bones from various fish species . Comparative Biochemistry and Physiology. 2(146): 395 – 401. https://doi.org/10.1016/j.cbpb.2006.11.020
24. Klompong, V., S., Benjakul, D., Kantachote and F., Shahidi . 2009 . Characteristics and use of yellow stripe trevally hydrolysate as culture media. Journal of Food Science, 74(3), 219 – 225. http://dx.doi.org/10.1111/j.17503841.2009.01213.x
25. Maktoof , A. , A. , Elherarlla , R., Jafar and E., Ethaib. 2020 . Identifying the nutritional composition of fish waste, bones, scales, and fins . International Conference of Pure and Engineering Sciences. 10(3): 145 – 156. http://dx.doi.org/10.1088/1757899X/871/1/012013
26. Malikil, K., H., Jamsari, and M., Yetti. 2023. New growth medium for culturing Lactic Acid Bacteria as probiotic consortium isolated from fermented fish (Budu). J. Anim. Feed Res. 13(3): 199 – 203. https://dx.doi.org/10.51227/ojafr.2023.30
27. Marie , S. , G., Paula , R.N., Ana , G.F., Carl and A.Z., Ahmad. 2021. Identifying the essential nutritional requirements of the probiotic bacteria Bifidobacterium animalis and Bifidobacterium longum through genome-scale modeling . npj Systems Biology and Applications. 7(47):1 – 15. http://dx.doi.org/10.1038/s41540021002074
28. Mejia – Gomez, C.E. and N., Balcazar. 2019. Isolation, characterisation and continuous culture of Lactobacillus spp. and its potential use for lactic acid production from whey. Food Science and Technology . 40(4): 1021 – 1028. http://dx.doi.org/10.1590/fst.29619
29. Meng , L., L., Shuang , L., Gefei, F. , Xuejing, Q., Yali, A., Yanan, L., Zhang , Z., Xingming, K., Huang and F., Zhen. 2022. Nutrient requirements of Lactobacillus acidophilus LA-5 and their application to fermented milk . J. Dairy Sci. 104:138–150. http://dx.doi.org/10.3168/jds.2020-18953
30. Mohammad, M.A. 2023 . Effect of adding garlic allium sativumpoder in diet on hematologicl, biochemial and histopathological criteria of Cyprinus Carpio L. Iraqi Journal of Agricultural Sciences. 54(4):1040 - 1049. https://doi.org/10.36103/ijas.v54i4.1793
31. Muhsin, S.N. and A.F., Hassan. 2019. The protective effect of Lactobacillus against ciprofloxacin and levofloxacin associated diarrhea in sample of iraqi patients. Iraqi Journal of Pharmaceutical Sciences. 28(2): 174 – 179. https://doi.org/10.31351/vol28iss2pp174179
32. Najem, E.S., O.G., Jalil, O.S, Majeed and W.S., Hassan. Isolation and identification of Pathogenic Bacteria from Cyprinus carpio L. by Vitek 2 System. Diyala Journal for Veterinary sciences, 2(2), 62 – 70.
33. Nurdiani , R., M., Ramadhan, A., Asep and M., Firdaus. 2022. Characteristics of fish protein hydrolysate from Mackerel (Scomber Japonicus) by products . Journal of Hunan University Natural Sciences.49(1): 75 – 83. http://dx.doi.org/10.55463/issn.16742974.49.1.10
34. Oscar , j. , J., Pedro and S., Liliana . 2019 . Review of Lactobacillus in the food industry and their culture media . Revista Colombiana de Biotecnología . 25(2):63 – 76. http://dx.doi.org/10.15446/rev.colomb.biote.v21n2.81576
35. Peter , J. , M. A. , Bechtel , J. M., Watson, L., Karen and M. B., John. 2019 . Properties of bone from Catfish heads and frames . Food Sci Nutr. 7(8):1396–1405. http://dx.doi.org/10.1002/fsn3.974
36. Petrut , S. , E., Rusu, I.S., Tudorache, D. , Pelinescu, I., Sarbu, I., Stoical and T., Vassu.2019. Influence of various carbon sources on growth and biomass accumulation of some lactic acid bacteria strains. Rev. Chim. 70(7):2434 – 2438. http://dx.doi.org/10.37358/RC.19.7.7356
37. Raji , R.A., K.O., Ebere and S., Oludayo 2022. Lactic acid bacteria and the food industry - a comprehensive review . International Journal of Health Sciences and Research.12(5): 128 – 142. http://dx.doi.org/10.52403/ijhsr.20220516
38. Ramezani Z. , E. , Rajabzadeh Ghatarmi, S.F., Hosseini and J.M., Regenstein. 2020 . Functional properties and antioxidant activities of protein hydrolysates from orangefin ponyfish (Photopectoralis bindus) . Iranian Journal of Fisheries Sciences , 19(6): 3001 – 3017. https://doi.org/10.22092/IJFS.2020.122930
39. Rosmawati , E.A., T., Abu Bakar and I.S., Muhammad .2018. Chemical composition, amino acid and collagen content of Snakehead (Channa striata) fish skin and bone . Scientific Research Journal .5(1):1-4. http://dx.doi.org/10.1007/s12562-01812488
40. Saeed, A., G., Rabin, O., Sulaiman, K., Albert and A., Salam. 2019 . Cultivation media for lactic acid bacteria used in dairy products . Journal of Dairy Research . 86(4): 490 – 502. http://dx.doi.org/10.1017/S002202991900075X
41. Setijawati , D. , A., Abdul, S.Y. , Hefti, D., Wardani , M.D. , Pratomo , D., Ersyah and H., Nurul. 2019 . Characteristics of peptone from the Mackerel (Scomber japonicas) head by-product as bacterial growth media . Biosc.Biotech.Res, 12 (4), 829 – 836. http://dx.doi.org/10.21786/bbrc/12.4/1
42. Shimadzu . 2019 . Simultaneous analysis of amino acids using automatic pretreatment function of prominence TM-i integrated LC system . Application News No. L529A .
43. Srikanya , A., K., Dhanapal, K., Sravani1, K., Madhavi and G., Kumar. 2017. A Study on optimization of fish protein hydrolysate preparation by enzymatic hydrolysis from Tilapia fish waste mince . Int.J.Curr.Microbiol.App.Sci 6(12): 3220 – 3229.
44. Shukry, A. Y., I. H. Alkafagy, and M. S. Al-Khshali. 2020. Relationship of the leptin hormone gene with some of the growth characteristics of common carp Cyprinus carpio L. Plant archives, 20(1): 1225-1230.
45. Tanoto, J., M.N., Florecita, M., Sanaky and A., Sugata. 2023 . Antibacterial activity of Lactobacillus plantarum F75 against Escherichia coli and Staphylococcus aureus. AIP Conf. Proc, 2614(1), 1 – 15.
46. Tessema, A., A., Getahun, S., Mengistou, T., Fetahi and E., Dejen . 2020. Reproductive biology of common carp (Cyprinus carpio Linnaeus, 1758) in Lake Hayq, Ethiopia. Fisheries and Aquatic Sciences , 23(16),1 – 10. http://dx.doi.org/10.1186/s41240-020-00162x
47. Tyas,U., A., Cindarbhumi, M., Khuangga , E. , Rahayu, M., Nur Cahyanto,S., Nurfiyani and E., Zulaichah. 2020 . Preparation of indigenous lactic acid bacteria starter cultures for large scale production of fermented milk . Digital Press Life Sciences .2(10):1 – 7. http://dx.doi.org/10.29037/digitalpress.22327
48. Vignesh, R., G., Anbarasi, M.P., Arulmoorthy, K., Mohan, A.C., Rathiesh and M., Srinivasan . 2015. Variations in the nutritional composition of the head and bone flours of tilapia (Oreochromis mossambicus) adapted to estuarine and freshwater environments . Journal of Microbiology, Biotechnology and Food Sciences . 4(4): 358 – 364. http://dx.doi.org/10.15414/jmbfs.2015.4.4.358-364
49. Yarlina, V.B., M., Djali and R., Andoyo. 2019. A review of protein hydrolysis fermented foods and their potential for health benefits . International Conference on Food and Bio-Industry. 8(15), 1 – 7 .
50. Yousef, T.A., and M. S. Al-Khshali. 2023. Relationship of growth hormone receptor gene with some of productive traits of common carp Cyprinus carpio. Iraqi Journal of Agricultural Sciences, 54 (3): 777- 783. https://doi.org/10.36103/ijas.v54i3.1760
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