CHARACTERIZATION OF UREASE EXTRACTED UNDER THE OPTIMUM CONDITIONS FROM SOME LOCAL PLANT SEEDS
DOI:
https://doi.org/10.36103/t5avmh82Keywords:
Cotton, plants, enzyme, extractionAbstract
This study was aimed to determine the optimum conditions of urease extracted from different plant sources and evaluate its biochemical properties. Eighteen types of plants commonly used namely Tomato, Chickpea, Sesame, Mustard, Lebbeck, Watermelon, Soybean, Cotton, Male Iraqi berries, Female Iraqi berries, Indian berries, Pumpkin, Muskmelon, Legumes, Cowpea, Radish, Pisum and Phaseolus were taken and tested for the presence and activity of urease. Cotton (Gossypium barbadense) was selected as the plant source with highest specific enzymatic activity among them (2.51 U/mg protein) when extracted the enzyme after ground seeds using 0.15 M of tris base buffer pH 8.5 at a ratio 1:10 (w:v) for 15 min. Also the results showed that urease was stable in pH 8, and has the highest activity at 37 oC at 60 min of reaction time. .
References
Amaal, A. H., Sahar, I. H., and Mohanad, J. M-Ridha. 2023. Determination of the optimum conditions for urease extracted from some local plants. Iragi Journal of Agricultural Sciences. 54(3):647-656. https://doi.org/10.36103/ijas.v54i3.1776
Babazadeh, N. S., Salehabadi, H., Zeidabadi, F., Souri, E. and M. Amanlou. 2017. Study of urease inhibitory activity by medicinal plants extract based on new catalyst for Berthelot reaction and Taguchi experimental design. Journal of the Iranian Chemical Society 15(Suppl):1-8. https://doi.org/10.1007/s13738-017-1255-1
Barrios, A.M. and S.J Lippard. 2000. Interaction of urea with a hydroxide bridged dinuclear nickel center: an alternative model for the mechanism of urease. Journal of the American Chemical Society. 122: 9172-9177. https://doi.org/10.1021/ja000202v
Bedan, D.S. 2020. Extraction, precipitation and characterization of urease from Vicia faba L. Al-Mustansiriyah Journal of Science. 31(1). https://doi.org/10.23851/mjs.v31i1.555
Bradford, M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein using the principle of protein-dye binding. Analytical Biochemistry Journal. 72: 248-254. https://doi.org/10.1016/0003-2697(76)90527-3
Cassone, A., De Bernardis, F., Mondello, F., Ciddia, T. and L. Agatensi. 1987. Evidence for a correlation between proteinase secretion and vulvoriginal candidsis. Journal of Infectious Disease. 156: 777-783. https://doi.org/10.1093/infdis/156.5.777
Daniel, R.M., Danson, M. J., Eisenthal, R., Lee, C. K. and M. E. Peterson. 2008. The effect of temperature on enzyme activity: new insights and their implications. Extremophiles Journal. 12:51–59. https://doi.org/10.1007/S00792-007-0089-7
Department of health and aging therapeutic good administration. 2011. Version 10. Guidance on Equivalence of Herbal Extracts. Australian Government. https://www.tga.gov.au/sites/default/files/cm-herbal-extracts-equivalence
EL-Hefnawy, M. E., M., Sakran, A.I., Ismail and Aboelfetoh, E.F. 2014. Extraction, purification, kinetic and thermodynamic properties of urease from germinating Pisum Sativum L. seeds. BMC Biochemistry Journal. 15:15. https://doi.org/10.1186/1471-2091-15-15
Ghazi, M. A. S., S. I. Hussein, S. D. Abbass, A. L. Ibrahim, and D. K. Abbas. 2021. Degradation of reactive dyes using immobilized peroxidase purified from Nigella sativa. Iragi Journal of Agricultural Sciences. 52(6):1365-1374. https://doi.org/10.36103/ijas.v52i6.1476
Hanaa, N. S., and S. I. Hussein. 2022. Assessment of purified collagenase inhibition activity from Staphylococcus aureus by some local plants extract. Iragi Journal of Agricultural Sciences. 53(5):1035-1047. https://doi.org/10.36103/ijas.v53i5.1617
Hirayama, C., Sugimura, M., Saito, H. and M. Nakamura. 2000. Purification and properties of urease from leaf of mulberry, Morus alba. Phytochemistry Journal. 53: 325-330.
https://doi.org/10.1016/S0031-9422(99)00521-X
Hussein, S. I. 2024. Purification and characterization of amylase extracted from local wheat. Iragi Journal of Agricultural Sciences. 3:54(5): 1183-1192. https://www.researchgate.net/publication/377489046
Hussein, S. I., Khalaf, A. F., Sameh M. A. and M. T. Salah. 2021. Determination of the optimum conditions for urease inhibition extracted from some local plants. Iraqi Journal of Agricultural Sciences. 52(4):802-809. https://doi.org/10.36103/ijas.v52i4.1389
Jabri, E., Carr, M.B., Hausinger, R.P. and P.A. Karplus. 1995. The crystal structure of urease from Klebsiella aerogenes. Science Journal. 268: 998-1004. https://doi.org/10.1126/science.7754395
Kappaun, K., Piovesan, A. R., Carlini C. R., and R. Ligabue-Braun. 2018. Ureases: Historicalaspects, catalytic, and non-catalytic properties - A review. Journal of Advanced Research. 13: 3-17. https://doi.org/10.1016/j.jare.2018.05.010
Krajewska, B. 2009. Urease I. Functional, catalytic and kinetic properties: a review. Journal of Molecular Catalysis B.59: 9-21. https://doi.org/10.1016/j.molcatb.2009.01.003
Lyer, P. K., Priya, V. V. and R. Gayathri. 2018. Assessment of urease activity in Pisum sativum seeds. Drug Invention Today.10: (9). https://www.researchgate.net/publication/327202311
Miyagawa, K., Sumida, M., Nakao, M., Harada, M., Yamamoto, H., Kusumi, T., Yoshizawa, K., Amachi T. and T. Nakayama 1999. Purification, characterization, and application of an acid urease from Arthrobacter mobilis. Journal of Biotechnology .68: 227–236. https://doi.org/10.1016/S0168-1656(98)00210-7
Mizobutsi, G. P., Finger, F.L., Ribeiro, R.A., Puschmann, R., Neves, L.D.M. and W.F.D. Mota. 2010. Effect of pH and temperature on peroxidase and polyphenol oxidases activities of litchi percarp. Journal of Science and Agriculture. 67(2):213-217. https://doi.org/10.1590/S0103-90162010000200013
Mobley, H.L., Island, M.D. and R.P. Hausinger, 1995. Molecular biology of microbial ureases. Microbiology Review Journal.59: 451-480. https://doi.org/10.1128/mr.59.3.451-480.1995
Modolo, L. V., Souza, A. X., Horta, P. D. Araujo, D. and A. de. Fatima, 2015. An overview on the potential of natural products as ureases inhibitors : A review q. Journal of Advanced Research. 6(1): 35-44. https://doi.org/10.1016/j.jare.2014.09.001
Pandy, P.C. and V. Pandy, 1991. Urease purification from the seeds of Cajanus cajan and its application in a biosensor construction. Applied Biochemistry and Biotechnology Journal. 31: 247-251. https://doi.org/0273-2229913105-0247k000
Predescu, N. C., Papuc, C., Nicorescu, V., Gajaila, I., Goran, G. V., Petcu, C. D. and G. Stefan. 2016. The influence of solid-to-solvent ratio and extraction method on total phenolic content, flavonoid content and antioxidant properties of some ethanolic plant extracts. Revista de Chimie -Bucharest- Original Edition. 67(10):1922-1927. https://www.researchgate.net/poblication/309740361
Qin, Y. and Cabral, J.M.S. 2002. Review properties and applications of urease. Biocatalys and Biotransformation Journal. 20(1):1–14. https://doi.org/10.1080/10242420290003236
Rachhpalsingh, N. and Nye, P. H. 2006. pH: The effect of soil-pH and high urea concentrations on urease activity in soil. Journal of Soil Science. 35(4):519-527. https://doi.org/10.1111/j.1365-2389.1984.tb00609.x
Segal, I. Biochemical Calculations 1976. 2ed edition. John Wiley and sons Inc. New York. https://www.scirp.org/reference/609629
Sekichi, F., K., Fukami, K., Takaoka, K., Miyake, T., Yamamoto, S., Tanaka, K., Ohn, H., Ishikura, M., Naruse and A. Kawabata 2009. Correlation between the abundance of Helicobacter pylori-derived urease and expression levels of PAR1 or PAR2 in the gastric tissue isolated from gastric cancer patients. Journal of Pharmacology and Science. 109:172P–172P. https://www.biomedcentral.com/1471-2091/15/15
Teba, J. H., and S. I. Hussein. 2022. Development of bioprocesses for production and purification of L-asparaginase from staphylococcus aureus, and invitro efficacy against human breast cancer cell line. Iragi Journal of Agricultural Sciences. 53(6):1525-1538. https://doi.org/10.36103/ijas.v53i6.1668
Whitaker, J. R. and R. A. Bernard, 1972. Experiments for an Introduction of Enzymology. The Wibber Press. Davis 1972. https://eclass.uowm.gr/modules/document/file.php/FOODQUAL105
Wilcox, A. A., Carroll, W.E., Sterling, R. E., Davis, H. A. and A. G. Ware. 1966. Use of the berthelot reaction in the automated analysis of serum urea nitrogen. Journal of Clinical Chemistry. 12(3): 151–157. https://pubmed.ncbi.nlm.nih.gov/5904232
Yi-Ywan, M., Chen, K., Anne C. and R. Burne. 1996. Streptococcus salivarius Urease: Genetic and biochemical characterization and expression in a dental plaque streptococcus. Infection and Immunity Journal. 585–592. https://doi.org/10.1128/IAI.64.2.585-592.1996
Zainab M. D. 2020. Urease activity level in crude extract from peels of some legumes and cucurbits. Journal of Global Pharma Technology. 12(2): 21-25. https://www.researchgate.net/publication/359221968
Zonia, L.E., Stebbins N.E. and J.C. Polacco. 1995. Essential role of urease in germination of nitrogen-limited arabidopsis-thaliana seeds. Journal of Plant Physiology. 107(4):1097–1103. https://doi.org/10.1104/pp.107.4.1097
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