DETERMINATION OF THE OPTIMUM CONDITIONS FOR UREASE EXTRACTED FROM SOME LOCAL PLANTS
DOI:
https://doi.org/10.36103/ijas.v54i3.1776Keywords:
Sesame, plants, urease enzyme, optimizationAbstract
In the current study, Seventeen types of plants commonly used namely (Chickpeas, Tomato, Soybean, Mustard, Baker, Lebbeck, Bean, Sesame, Male Iraqi berries, Female Iraqi berries, Indian berries, Potato, Radish, Legumes, peas, Watermelon, and Phaseolus were obtained and screened for urease activity, among these plants, Sesame was chosen with maximum enzymatic activity, and it had the highest productivity of urease enzyme (1.623 U/mg protein). The optimum extraction ratio represented by 1:10 (W: V) after 90 minutes and 0.8414U/mg protein. Sodium phosphate buffer (0.1 M, pH 7.0) was chosen as the best extraction buffer with specific activity 0.9004U/mg protein.
References
Annu, Garg A, Urmila. 2016. Level of Cd in different types of soil of Rohtak district and its bioremediation. Journal of Environmental Chemical Engineering. 4:3797–3802
Barrios, A.M. and S.J Lippard, 2000."Interaction of urea with a hydroxide bridged di-nuclear nickel center: an alternative model for the mechanism of urease," Journal of the American Chemical Society.122: 9172-9177
Becker-Ritt, A.B., A.H. Martinelli, S. Mitidieri, V. Feder, G.E. Wasserman , M.H. Vainstein , J.T. Oliveira , L.M. Fiuza, G. Pasqualli and C.R. Carlini. 2007. "Antifungal activity of plant and bacterial ureases. Toxicon Journal. 50: 971-983
Bradford, M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein using the principle of protein-dye binding. Analytical Biochemistry Journal. 72: 248-254
Department of health and aging therapeutic good administration. 2011. Version 10. Guidance on Equivalence of Herbal Extracts. Australian Government
El-Hefnawy, M.E., M. Sakran, A. Ismail, and E.F. Aboelfetoh. 2014. Extraction, purification, kinetic and thermodynamic properties of urease from germinating Pisum Sativum L. seeds. BMC Biochemistry Journal. 15:15
Florkin, M. and M. Howaders. 1960, “Comparative Biochemistry”, New York: Academic Press
Ghazi, M. A., S. I. Hussein, S. D. Abbass, A. L. Ibrahim, and D. K. Abbas. 2021. Degradation of reactive dyes using immobilized peroxidase purified from Nigella sativa. Iraqi Journal of Agricultural Sciences. 52(6):1365-1374. https://doi.org/10.36103/ijas.v52i6.1476
Granick, S. 1937. Urease distribution in plants: General methods. Plant Physiology Journal. 12(2): 471–486
Guisan, J.M. 2014. Immobilization of enzyme and cells.2nd ed. Madrid, Spain
Hanaa, N. S. and S. I. Hussein.2022. Assessment of purified collagenase inhibition activity from Staphylococcus aureus by some local plants extract. Iraqi Journal of Agricultural Sciences. 53(5):1035-1047. https://doi.org/10.36103/ijas.v53i5.1617
Hussein, S. I. 2018. Efficiency of immobilized polyphenol oxidase on some textile dyes degradation using batch operation system by packed bed bioreactor. Iraqi journal of agricultural sciences.50 (3):943-950. https://doi.org/10.36103/ijas.v50i3.711
Hussein, S.I, A. F. Kaluf, M. A. Sameh, M.A. and M. T. Salah, 2021. Determination of the optimum conditions for urease inhibition extracted from some local plants. Iraqi Journal of Agricultural Sciences.52(4):802-809. https://doi.org/10.36103/ijas.v52i4.1389
Iyer, P. K., V. V. Priya, and R. Gayathri, 2018. Assessment of urease activity in Pisum sativum seeds. Drug Invention Today Joyrnal.10: (9).
Kandeler, E. 1996. Ammonium. In: Schinner, F., O¨ hlinger, R., Kandeler, E., Margesin, R. (Eds.), Methods in Soil Biology. Springer, Berlin, Heidelberg, New York, pp. 406–408
Karmali, K. et al. 2004. The use of Fourier transform infrared spectroscopy to assay for urease from Pseudomonas aeruginosa and Canavalia ensiformis. Analytical Biochemistry Journal.331, 115-21
Khalil-ur-Rehman, M. Y., M. A. Sheikh, and M. Arshad, 1999. Extraction and Evaluation of peroxidase from Vegetable Source. International Journal of Agriculture and Biology. 3:170-173
Kochhar, A., A. K. Gupta, and N. Kaur, 1999. Purification and immobilization of inulinase from Aspergillus candidus for producing fructose. Journal of Science Food and Agriculture. 79: 549-554
Lal R.B., D. E. Kissel, M. L. Cabrera, and A.P. Schwab. 1993. Kinetics of urea hydrolysis in wheat residue. Soil Biology and Biochemistry Journal. 25(8):1033–1036
Liu, J.Y. Xu, Y. Nie and G.A. Zhao. 2012. "Optimization production of acid urease by Enterobacter sp. in an approach to reduce urea in Chinese rice wine. Bioprocess and Biosystems Engineering Journal. 35: 651-658
Lubbers M.W., S. B. Rodriguez, N. K. Honey, and R. J. Thornton: 1996 Purification and characterization of urease from Schizosaccharomyces pombe. Canadian Journal of Microbiology. 42(2):132–140
Mahmood, B., F. 2018. Extraction, Purification and Characterization of Laccase from Locally Isolate of Cladophora sp. and using in Decolariztion of Some Industrial Dyes. Thesis. University of Anbar, College of Education for Pure Science, Department.
Mirbod, F., R.A. Schaller and T. Cole. , 2002. "Purification and characterization of urease Isolated from the pathogenic fungus Coccidioides imitis. Medical Mycology Journal.40: 35-44
Miyagawa, K., M. Sumida, M. Nakao, M. Harada, H. Yamamoto, T. Kusumi, K. Yoshizawa, T. Amachi and T. Nakayama 1999. Purification, characterization, and application of an acid urease from Arthrobacter mobilis. Journal of Biotechnology. 68:227–236
Mizobutsi, G. P., Finger, F.L., Ribeiro, R.A., Puschmann, R., Neves, L.D.M. and W.F.D. Mota. 2010. Effect of pH and temperature on peroxidase and polyphenol oxidases activities of litchi percarp. Journal of Science and Agriculture. 67(2):213-217
Mohammed, T.M. Mohammed, M.A., Mohammed, S.A. and A. S. Fahmy, 1999 " Purification of urease from water melon seeds for clinical diagnostic kits. Bioresource Technology Journal. 68: 215-223
Muck RE: 1982 Urease activity in bovine feces. Journal of Dairy Science. 65(11):2157–2163
Nilsang, S., S. Lertsiri, M. Suphantharika, and A. Assavanig. 2005. Optimization of enzymatic hydrolysis of fish soluble concentrate by commercial proteases. Journal of Food Engineering. 70: 571-578.
Pandy, P.C. and V. Pandy, 1991. , "Ureaspurification from the seeds of Cajanus cajan and its application in a biosensor construction. Applied Biochemistry and Biotechnology. 31:247-251
Pervin, S., et al., Research & Reviews in. 2013
Rachhpalsingh, Nye. 1984. pH: The effect of soil-pH and high urea concentrations on urease activity in soil. Journal of Soil Science. 35(4):519–527
Segel, J. J. 1976. Biochemical Calculation. John Wiley and sons
Sieprawska, A.K., K. Rafinska, J.W. Skierska, and B. Buszewski, 2020. The Influence of Plant Material Enzymatic Hydrolysis and Extraction Conditions on the Polyphenolic Profiles and Antioxidant Activity of Extracts: A Green and E_cient Approach
Teba J. H. and S. I. Hussein. 2022. Development of bioprocesses for production and purification of L-asparaginase from Staphylococcus aureus, and invitro efficacy against human breast cancer cell line. Iraqi Journal of Agricultural Sciences. 53(6):1525- 1538. https://doi.org/10.36103/ijas.v53i6.1668
Tortora G. J., B. R. Funke, and C. L. Case, 2004. Microbiology, (8th ed.). Pearson Education, Inc. San Francisco, New York.
Whitaker, J. R. and R. A. Bernard, 1972. Experiments for an Introduction of Enzymology. The Wibber Press. Davis 1972
Zhao X,Wang M,Wang H, Tang D, Huang J, Sun Y, 2019. Study on the remediation of Cd pollution by the biomineralization of ureaseproducing bacteria. International Journal of Environmental Research and Public Health. 16(268):1–14
Zusfahair, Ningsih, D. R., A. Fatoni, and D. S. Pertiwi. 2018. Determination of Urease Biochemical Properties of Asparagus Bean (Vigna unguiculata ssp sesquipedalis L.). The 12th Joint Conference on Chemistry. IOP Conf. Series: Materials Science and Engineering 349 (2018) 012073 doi:10.1088/1757-899X/349/1/012073.
Downloads
Published
Issue
Section
License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
How to Cite
2.jpg)
https://orcid.org/0000-0002-5774-5906 2.jpg)
