EFFECT OF DIFFERENT LEVELS OF SALT AND DROUGHT STRESSES ON GENE EXPRESSION OF TWO TOLERANCE-DIFFERENT TOMATO CULTIVARS IN VITRO
DOI:
https://doi.org/10.36103/gc35eh69Keywords:
PEG, NaCl, proline, ions leakage, SDS-PAGE, abiotic stressAbstract
A lab experiment was conducted at the Plant Tissue Culture Lab / College of Agricultural Engineering Sciences / University of Baghdad. This experiment was aimed to investigate gene expression index in tomato (Solanum lycopersicum L.) after preparation of salt and simulated drought stresses. Two tomato cultivars were selected which claimed to exhibit different levels of tolerance toward abiotic stresses designated as salt-tolerant Yassamine (Y) and salt-sensitive GS12 (G) to assess the test. Seven day-old seedlings from both cultivars were grown in MS media supplemented with four concentrations of NaCl at 0, 50, 100 and 150 mM and four concentrations of PEG at 0, 10, 20, and 30% for 48 hours. The results were showed that Y cultivar exhibited more proline secretion and chlorophyll content when compared with G. In addition, Y cultivar showed less ion leakage and less affected by elevated abiotic stresses in term of seedling weight variation when compared to G counterparts. The SDS-PAGE gel analysis showed that Y cultivar showed more band intensity when compared with G suggested more corresponding gene expression of tolerant protein against abiotic stresses.
References
Ábrahám, E, C. Hourton-Cabassa, L. Erdei, and L. Szabados, 2010. Methods for Determination of Proline in Plants. In: Sunkar R. (eds) Plant Stress Tolerance. Methods in Molecular Biology (Methods and Protocols), 639: 317-331. Humana Press
Amjad, M, J. Akhtar, M. Anwar-ul-Haq, R. Ahmad, and M. Zaid, 2014. Characterization of comparative response to fifteen tomato (Lycopersicon esculentum Mill.) genotypes to NaCl stress. Journal of Agricultural Sciences and Technology. 16:851-862
Amini, A, A. Ehsanpour , Q.T Hoang, and J.S. Shin, 2007. Protein pattern changes in tomato under in vitro salt stress. Russ. J. Plant Physiol. 54:464–471
Annon, A.H. and I.J. Abdulrasool, 2020. Effect of gamma radiation and ethyl methanesulfonate (EMS) on potato salt stress tolerance in vitro. Iraqi Journal of Agricultural Sciences. 51(4): 982-990
Annon, A, K. Rathore , and K. Crosby, 2014. Overexpression of a tobacco osmotin gene in carrot (Daucus carota L.) enhances drought tolerance. In Vitro Cellular & Developmental Biology-Plant. 50(3): 299-306
Barthakur, S, V. Babu, and K.C. Bansal, 2001. Overexpression of osmotin induces proline accumulation and confers tolerance to osmotic stress in transgenic tobacco. J Plant Biochem Biotechnol. 10:31–37
Bhargava, S. and K. Sawant, 2013. Drought stress adaptation: metabolic adjustment and regulation of gene expression. Plant Breeding. 132: 21–32
Candar_Cakir B, E. Arican, and B. Zhang, 2016. Small RNA and degradome deep sequencing reveals drought‐and tissue‐specific micrornas and their important roles in drought‐sensitive and drought‐tolerant tomato genotypes. Plant Biotechnol J.14(8):1727–1746
Das M, H. Chauhan, A. Chhibbar, Q.M.R. Haq, and P. Khurana, 2010. High-efficiency transformation and selective tolerance against biotic and abiotic stress in mulberry, Morus indica cv. K2, by constitutive and inducible expression of tobacco osmotin. Transgenic Res. 20:231–246.
de Freitas, CDT, FCS Nogueira, IM Vasconcelos, JTA Oliveira, GB Domont, and MV Ramos, 2011. Osmotin purified from the latex of calotropis procera: biochemical characterization, biological activity and role in plant defense. Plant physiology and Biochemistry, 49(7):738-743
Ding Y, Y. Tao, C. Zhu, 2013. Emerging roles of microRNAs in the mediation of drought stress response in plants. J. Exp. Bot. 64: 3077– 3086
Florina F, V. Giancarla, P. Cerasela, and P. Sofia, 2013. The effect of salt stress on chlorophyll content in several Romanian tomato varieties. Journal of Horticulture, Forestry, and Biotechnology. 17(1): 363-367.
Florina F, 2012. Assessment of genetic diversity in a collection of local tomatoes by SDS-PAGE method. Journal of Horticulture, Forestry and Biotechnology. 16(3): 133-136.
Goel D, AK Singh, V Yadav, B Babbar, and KC Bansal, 2010. Overexpression of osmotin gene confers tolerance to salt and drought stresses in transgenic tomato (Solanum lycopersicum L.). Protoplasma 245:133–141
Horwitz, W, 2010. Official methods of analysis of AOAC International. Volume I, agricultural chemicals, contaminants, drugs/edited by William Horwitz. Gaithersburg (Maryland): AOAC International
Jambunathan, N, 2010. Determination and Detection of Reactive Oxygen Species (ROS), Lipid Peroxidation, and Electrolyte Leakage in Plants. In: Sunkar R. (eds) Plant Stress Tolerance. Methods in Molecular Biology (Methods and Protocols), vol 639: 291-297. Humana Press
Jacob P, H. Hirt, and A. Bendahmane, 2017. The heat‐ shock protein/chaperone network and multiple stress resistance. Plant Biotechnol J. 15: 405-414
Kahlaoui B, M. Hachicha, E. Misle, F. Fidalgo, and J Teixeira, 2018. Physiological and biochemical responses to the exogenous application of proline of tomato plants irrigated with saline water. Journal of the Saudi Society of Agricultural Sciences. 17(1):17-23
Khalifa N.S, 2012. Protein expression after NaCl treatment in two tomato cultivar differing in salt tolerance. Botanica. 54(2): 79-86
Knox, J, T. Hess, A. Daccache, and T. Wheeler, 2012. Climate change impacts on crop productivity in Africa and South Asia. Environmental Research Letters. 7(3): 32-34
Lawlor D.W. and G. Cornic, 2002. Photosynthetic carbon assimilation and associated metabolism in relation to water deficits in higher plants. Plant Cell Environ. 25: 275-294
Li, C. and B.H. Zhang, 2016. Micro, R.N.A.s in control of plant development. J. Cell Physiol. 231: 303–313
Li Q, HM Yu, XF Meng, JS Lin, YJ Li, and BK Hou, 2018. Ectopic expression of glycosyltransferase UGT76E11 increases flavonoid accumulation and enhances abiotic stress tolerance in Arabidopsis. Plant Biol. 20: 10-19
Murashige T and F Skoog, 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia plantarum 15(3): 473-497
Oh. SK, H,A, Jang, SS Lee, H,S, Cho, D. H. Lee, D, Choi, and S,Y Kwon, 2014. Cucumber Pti1-L is a cytoplasmic protein kinase involved in defense responses and salt tolerance. Journal of plant physiology. 171(10): 817-822
Ohama N, H Sato, K Shinozaki, and K Yamaguchi-Shinozaki, 2017. Transcriptional regulatory network of plant heat stress response. Trends Plant Sci. 22: 53-65
Pareek, A, SA Singla, SK Sopory, and A Grover, 2007. Analysis of salt stress-related transcriptome fingerprints from diverse plant species. Genomics-Assisted Crop Improvement. 5: 267-287
Parkh,i V, V. Kumar, G. Sunilkumar, LM Campbell, NK Singh, and KS Rathore, 2009. Expression of apoplastically secreted tobacco osmotin in cotton confers drought tolerance. Mol Breed. 23:625–639
Signorelli, S, F. Coitiño, O. Borsani, and J Monza, 2014. Molecular mechanisms for the reaction between OH radicals and proline: insights on the role as reactive oxygen species scavenger in plant stress. The Journal of Physical Chemistry B, 118(1):37-47
Sarwar, M, S Ahmad, M Chattha, M Alam, S Anjum, T Shafeeq, M Naseem, and A Mannan, 2019. Assesment of growth and productivity of cucumber (Cucumis sativus L.) genotypes under salt stress regime. Applied Ecology and Environmental Research. 17(5): 10793-10806
Seki, M, A. Kamei, K. Yamaguchi, and K. Shinozak, 2003. Molecular responses to drought, salinity and frost: common and different paths for plant protection. Current Opinion in Biotechnology. 14: 194–199
Song. N. H. and Y. J. Ahn, 2010. DcHsp17.7, a small heat shock protein from carrot, is upregulated under cold stress and enhances cold tolerance by functioning as a molecular chaperone. HortScience. 45:469-474
VanWallendael A, A Soltani, NC Emery, MM Peixoto, J Olsen, and DB Lowry, 2019. A Molecular view of plant local adaptation: incorporating stress-response networks. Annual Review of Plant Biology. 70(1): 559-583
Yordanov, I.,V, T Velikova, and T. Tsonev, 2003. Plant responses to drought and stress tolerance. Bulg. J. Plant Physiol. Special Issue: 187-206
Zhu, J.K, 2002. Salt and drought stress signal transduction in plants. Annu. Rev. Plant Biol. 53: 243-273.
Downloads
Published
Issue
Section
License
Copyright (c) 2024 IRAQI JOURNAL OF AGRICULTURAL SCIENCES
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
How to Cite
2.jpg)
2.jpg)
