MICROPROPAGATION AND ASSESSMENT OF GENETIC FIDELITY OF REGENERATE BY RAPD MARKERS OF SOLANUM NIGRUM
DOI:
https://doi.org/10.36103/tnx6m684Keywords:
medicinal plant, plant growth regulators, molecular markersAbstract
In vitro propagation approach has been adopted for micropropagation and conservation of Solanum nigrum (black nightshade) to guarantee its sustainable phyto-industry and research availability. Within 1 month, in vitro shoot differentiated was accomplished by culturing shoot tip, nodal segment, and leaf on MS medium enriched with various BA (Benzyl Adenine) concentrations. A significant difference was observed with shoot tips compared with nodal explants and leaf at the shoot induction percentage and mean number of shoot per explant. The highest percentage of shoot induction (100%) and average number of micro shoot per shoo tip (9.200 shoot) were recorded on MS medium fortified with 1 mg/l BA. The findings showed that a satisfactory rooting percentage (100%) was recorded when I mg/l IBA (Indole Butyric Acid) added to half MS medium with an average number of roots (2.600) root/shoot, a mean root length is 8.600cm. rooted plantlets could be successfully acclimatized in the shade house with 100% survival rate. The genetic stability of the micropropagated plants investigated by the RAPD markers indicated monomorphic and true to type when compare with the original plant.
References
Alansi, S.; F. AL-Qurainy; M. Ndeem; S. Khan; A. AL-Shameri; M. Tarroum and A. R., Gaffar. 2020. An efficient micropropagation protocol via indirect organogenesis from callus of economically valuable crop date palm (Phoenix dactylifera L.) cultivars “Sagai and Khalas”. Pak. J. Bot., 52(6):2021-2030.
AL-Dabagh, F. M. K. and M. I. Salih. 2020. Chia seed as a source of in vitro establishment of Salvia hispanica L. plants. Iraqi Journal of Agricultural Sciences. 51(3):976-981. https://doi.org/10.36103/ijas.v51i3.1053
Al-Jubori, M.T., AL-Dabbagh, F. M. K. and E. W. Al-Ani. 2023. Optimizing media sterilization via chlorine dioxide and autoclaving of Paulowni micropropagation. Irqi Journal of Agricultural Sciences, 54(6):1737-1745. doi: 10.36103/ijas.v54i6.1872
Allison, D. O.; H. C. Pilkey and W. A. Powell . 2020. Improving Ex Vitro rooting and acclimatization techniques for micropropagated American Chestnut. Journal of Environmental Horticulture, 38 (4): 149–157
Al-Qurainy, F.; Nadeem, M.; Khan, S.; Alansi, S.; Tarroum, M.; Al-Ameri, A.A.; Gaafar, A.R.Z. and A. Alshameri. 2018. Rapid plant regeneration, validation of genetic integrity by ISSR markers and conservation of Reseda pentagyna an endemic plant growing in Saudi Arabia. Saudi J. Biol. Sci. 25:111–116.
Antony, J.J.J.; Shamshir, R.A.; Poobathy, R.; Chew, B.L. and S. Subramaniam. 2015. Somaclonal variations were not induced by the cryopreservation: levels of somaclonal variations of in vitro and thawed protocorms of Dendrobium Bobby Messina analysed by SCoT and TRAP DNA markers. South African J. Bot. 100: 148–157
Chirumamilla, C. P.; Chaitanya Gopu; Phanikanth Jogam and S. Taduri. 2020. Highly efficient rapid micropropagation and assessment of genetic fidelity of regenerants by ISSR and SCOT markers of Solanum khasianum. Plant Cell, Tissue and Organ Culture (PCTOC). https://doi.org/10.1007/s11240-020-01964-6
Choudhary, A. K.; P. Barman; S. Subramaniam; K. M. Pathi and S. Kaushik. 2014. In Vitro culture of economically important plant: Solanum nigrum. The Scitech Journal, 1(4): 23-25
Fadladeen, L. H., and R. S. Toma , 2020. Embryo culture and in vitro clonal propagation of oak (Quercus aegilops L.). Iraqi Journal of Agricultural Sciences, 51(1), 347-355. https://doi.org/10.36103/ijas.v51i1.934
Faisal, M.; A., Naseem; A., Mohammad and A., Abdulrahman. 2018. Auxin-cytokinin synergism in vitro of Ruta graveolens using shoot tip meristems. Saudi Journal of Biological Sciences. 25: 273-277
Hatzilazarou, S; K. Stefanos; T. Nendou and E. Athanasios. 2021. Conservation, regeneration and genetic stability of regenerants from alginate-encapsulated shoot explants of Gardenia jasminoides Ellis. Polymers, 13, 1666. https://doi.org/10.3390/polym13101666
Hong Yao, L.; T., Xin ; Y., Zhuo ; L., Huanyong; S., Chongzhen; W., Xiyang and X., Dan. 2020. Two novel polysaccharides from Solanum nigrum L. exert potential prebiotic effects in an in vitro fermentation model. International Journal of Biological Macromolecules. https://doi.org/10.1016/j.ijbiomac.2020.05.121
Hussein, A. E. and J. M. S. Jubrael. 2021. AFLP marker in genetic diversity assessment of fig (Ficus carica L.) populations in Kurdistan region-Iraq. Iraqi Journal of Agricultural Sciences, 52(4):859-867. https://doi.org/10.36103/ijas.v52i4.1393
Hussain, SA; N. Ahmad and M. Anis. 2018. Synergetic effect of TDZ and BA on minimizing the post-exposure effects on axillary shoot proliferation and assessment of genetic fidelity in Rauvolfia tetraphylla L. Rend Lincei. Sci. Fis. Nat., 29:109–115
Jogam, P.; D. Sandhya; M. S. Shekhawat; A. Alok; M. Manokari; S. Abbagani and V. R. Allini. 2020. Genetic stability analysis using DNA barcoding and molecular markers and foliar micro-morphological analysis of in vitro regenerated and in vivo grown plants of Artemisia vulgaris L. Industrial Crops and Products, 151:11247: 1-9
Kavitha, M.S.; E.G. Wesely and P. Mehalingam. 2012. Direct multiple shoot regeneration from shoot tip and nodal explants of Solanum nigrum L. A medicinal herb. Journal of Ornamental and Horticultural Plants, 2 (2): 65-72
Laila S. Younes; S., Rida A.; T., Reham W.; Q., Tamara S. and H., Fayha. 2019. Micropropagation of black nightshade (Solanum nigrum L.): A promising medicinal plant in Libya. The Libyan Journal of Agriculture, 24(2): 85-96
Lalia, S.; J. P.; P. T. Awasthi and G. Singh. 2007. Effect of IBA and NAA on rooting potential of stooled shoots of guava (Psidium guajava L.) cv. Sardar. Acta Hort., 735: 193-196.
Mamdouh, D; A., Mahgoub; M., Gabr; A., Ewais, E. and L., Smetanska. 2021. Genetic stability, phenolic, flavonoid, ferulic acid contents and antioxidant activity of micropropagated Lycium schweinfurthii. Plants. 10(10): 2089.
Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant, 15:473-497.
Oliya, B.K.; K., Chand; S., Thakuri, L.; K., Baniya, M.; ; K., Sah, A. and B., Pant. 2021. Assessment of genetic stability of micropropagated plants of Rhynchostylis retusa L. using RAPD markers. Sci. Hortic., 281, 110008
Osama. S.S. 2022. Micropropagation of grapevine (vitis vinifera L.) cvs. Red globe and superior. Irqi Journal of Agricultural Sciences, 53(4): 833-849. doi: 10.36103/ijas.v53i4.1596
Pirek, P.L.M. 1987. In vitro culture of higher plants. Handbook. Martinus Nijhoff Publishers, Dordrect. pp: 344.
Rathore, M.S.; G. Mastan, S.; P., Yadav; Bhatt, V.; D., Shekhawat N.S. and J. Chikara. 2016. Shoot regeneration from leaf explants of Withania coagulans (stocks) Dunal and genetic stability evaluation of regenerates with RAPD and ISSR markers. South African J. Bot. 102: 12–17
Rathore, V. and D. Gupta. 2013. In vitro Clonal Propagation of Solanum nigrum. Research Journal of Chemical and Environmental Sciences, 1(2): 32-33
Rawat, J.M.; B. Rawat and R. K. Agnihotri; A. Chandra and S. Nautiyal. 2013. In vitro propagation, genetic and secondary metabolite analysis of Aconitum violaceum Jacq. : A threatened medicinal herb. Acta Physiol. Plant. 35: 2589–2599
Saito, K. and H. Mizukami. 2002. Plant cell cultures as producers of secondary compounds. In: KM Oksman-Caldentey, WH Barx (eds.). Plant biotechnology and transgenic plants. Marcel Dekker Inc., New York, 77-108.
Salih, M. I.; F. M. K. Al Dabagh; L. M. H. AL-Mamoori and S. F. H. Kassad, 2018. The production of Abrus precatorius plants by In vitro micropropagation. Research Journal of Pharmaceutical, Biological and Chemical Sciences. 9(5): 218-223
Salih, M. I. and Al Dabagh, F. M. K. 2021. Comparative analysis of some phenolic acids of in vitro and in vivo grown plant leaves of Salvia hispanica. Irqi Journal of Agricultural Sciences, 52(1), pp. 189-195.DOI:https://doi.org/10.36103/ijas.v52i1.1250
Salman, O. N. , Al-Hayany, A. A. . and Ibrahim, K. M. 2022. Assessment of the success of micro grafting clementine timing on sour orange. Irqi Journal of Agricultural Sciences,53(4), PP. 825-832.
doi: 10.36103/ijas.v53i4.1595
Sarethy, I. P.; A., A. Kashyap; U., Bahal; N. Sejwal and R. Gabrani. 2014. Study of liquid culture system for micropropagation of the medicinal plant Solanum nigrum L. and its effect on antioxidant property, Acta Physiologiae Plantarum, 36(11): 2863-2870
Särkinen, T.; P., Poczai,.; E., Barboza G.; M., van der Weerden G.; M., Baden and S. Knapp. 2018. A revision of the old world black nightshades morelloid clade of (Solanum L.), Solanaceae. Phyto Keys, 106: 1–223
Shaik, S.; K. Goordiyal and M.P. Watt. 2018. The effects of micropropagation, acclimatization substrate and physiological age on the growth and Ca and Fe content of selected Solanum nigrum genotypes. South African Journal of Botany, 118, 19: 2–202,
Sharma, J.; S. Sharma; R. Shankarayan and S. Mallubhotla. 2021. Enhancement of solasodine content using signal molecules in shoot cultures of Solanum nigrum. Plant cell biotechnology and molecular biology. 22(11-12): 136-142.
Strydem, D.K. and H. T. Hartman. 1960. Effect of indole butyric acid on respiration and nitrogen metabolism in Marianna 2624 plum soft wood cuttings. Proc. Am. Soc. Hortic. Sci., 16:125-133.
Tikendra, L.; A., Thoungamba and P. Nongdam. 2019. Molecular genetic homogeneity assessment of micropropagated Dendrobium moschatum Sw. - A rare medicinal orchid, using RAPD and ISSR markers. Plant Gene, 19, 100196: 1-11
Toma, R. S. 2022 . Minitubers production of four potato (Solanum tuberosum L.) cultivars by tissue culture technique. Iraqi Journal of Agricultural Sciences, 53(5), 1058- 1066. https://doi.org/10.36103/ijas.v53i5.1619
Vemula, S; T. Koppula; P. Jogam and M. Mohammed. 2019. In vitro high frequency multiplication and assessment of genetic fidelity of Corallocarpus epigaeus: an endangered medicinal plant. Vegetos, 33: 63–73
Venugopal, R. B.; C. P. Kaviraj; S. Rao; F. T. Z., Jabeen and G. Kiran. 2005. Plant regeneration and in vitro flowering from leaf and nodal explants of Solanum nigrum L, An important medicinal plant. Plant Cell Biotechnology and Molecular Biology. 6 (1/2): 17-22
Vijendra, P.D.; Jayanna, S.G.; Kumar, V.; Gajula, H.; Rajashekar, J.; Sannabommaji, T.; Basappa, G. and C. M. Anuradha. 2017. Rapid in vitro propagation of Lucas aspera Spreng. A potential multipurpose Indian medicinal herb. Ind. Crop. Prod. 107: 281–287
Yasir, S. S.; R. M. Hamad and S. I. Neamah. 2022. Role of dimethyl sulfate on biochemical characteristics of Fragaria ananassn Duch under salinity stress in vitro. Iraqi Journal of Agricultural Sciences. 53(1):111-121. https://doi.org/10.36103/ijas.v53i1.1514
Yuan Yuan, C.; Y., Deng; K., Zheng; X., Hu; M, Zhu; X., Deng and X., I. Ruchun. 2019. An efficient micropropagation protocol for an endangered ornamental tree species (Magnolia sirindhorniae Noot. & Chalermglin) and assessment of genetic uniformity through DNA markers. Scientific Reports. 9: 9634: 1-10.
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