• Ghufran M.M. Al-ghanimi
  • Alrubeii A. M. S.




meat spoilage, shelf life of meat, bacterial contamination.


This study was aimed to evaluate the effect of elastin hydrolysates on the microbial content and sensory evaluation of ground beef refrigerated at 2°C for 12 days. The study included 10 treatments: T1 without addition, T2 addition (1000ppm/kg meat) and T3 addition (500ppm/kg meat) of elastin hydrolysates produced by the elastase enzyme (E.H.E).T4 addition (1000ppm/kg meat) and T5 addition (500ppm/kg meat) of Elastin hydrolysates produced by the collagenase enzyme (E.H.C), T6 addition (1000ppm/kg meat) and T7 addition (500ppm/kg meat)of elastin hydrolysates produced by the trypsin enzyme (E.H.T), T8 addition and (1000ppm/kg meat) T9 addition (500ppm/kg meat) of elastin hydrolysate produced by the pepsin enzyme(E.H.P) and T10 added to the antioxidant BHA (0.01) Per kg of meat. The treatments were stored cold for periods of 1, 3, 6, 9 and 12 days, and some laboratory tests were conducted on them that showed the effect of elastin hydrolysates when their effectiveness was tested against Escherichia coli Staphylococcus aureus bacteria. When hydrolysates were added to cold-stored minced beef, the lowest number of bacteria was recorded, as they decreased the logarithm. Total Plate Count TPC37, total psychrophilic count TPC4, and E.coli bacteria. Addition treatments also recorded significant increase in degrees of tenderness, flavor, juiciness, and general acceptability.


Abbas, A. A., and K. A. Shakir. 2021. Evaluation of antioxidant functionality of fish collagen enzymic hydrolysate. Iraqi Journal of Agricultural Science, 52(4): 876-884.‏ https://doi.org/10.36103/ijas.v52i4.1395

Abed, I. J., M. E. Ahmed, and MH. S. AL-Shimmary. 2021. Rosemary volatile oil as a preservative agent in some canned meat fods. Iraqi Journal of Agricultural Sciences, 52(1); 155-162. https://doi.org/10.36103/ijas.v52i1.1247

Ahmad, D. E., and A. S. Al-Hani. 2016.‏"Estimating function supply for red meat in Iraq for the period 1995-2013." Iraqi Journal of Agricultural Sciences 47.6. doi:www.iasj.net/iasj/article/127360

Ahmed Al-Zubaidi, L., Al-Rubeii, A. M., and A. S. Al-Salmany. 2021. Effect of Cinnamon and Turmeric Nanoparticles Extract on microorganisms of Fresh Ground Beef During Cold Storage. In IOP Conference Series: Earth and Environmental Science 910(1):012058. IOP Publishing.‏ DOI 10.1088/1755-1315/910/1/012058

Ahmed S. M. Al-Salmany, and A. M. S. AL-Rubeii. 2020. Effect of Cinnamon and Turmeric Nanoparticles Extract In Quality Characteristics Of Ground Beef During Freeze Storage. Plant Archives 20(1):350356.‏ DOI 10.1088/1755-1315/910/1/012058

AL-Ghanimi, G. M. M., and A. S. AL-Rubeii. 2020. Effect of antioxidant potential of Astaxanthin and Allyl isothiocyanate in quality characteristics of raw ground beef meat during cold storage. Plant Archives, 20(1):673-679.

DOI: 10.1088/1755-1315/910/1/012074

Al-Ghanimi, G. M., and A. M. Al-Rubaii. 2023. Study of some Functional Properties of Elastin Extracted from Bovine Leather. In IOP Conference Series: Earth and Environmental Science 1214(1):012036. IOP Publishing. doi:10.1088/1755-1315/1214/1/012036

Alrubeii, A. M., and M. M. Alalaq 2018. The bio-preservation of buffalo meat manufactured (pastrama) by using lactobacillus plantarum bacteria. The Iraqi journal of agricultural sciences, 49(1):152-159. https://doi.org/10.36103/ijas.v49i1.219

Al-Rubeii, A. M., and H. G. Zahir. 2019. Effect of adding different Levels of Flaxseed powder as a source of Omega-3 on the chemical characteristics of Karadi Carcass Lambs. Basrah Journal of Agricultural Sciences, 32, 194-206. DOI:10.37077/25200860.2019.160

Al-Wasidi, A. S., A. M., Naglah, M., Refat, S. M., El-Megharbel, Kalmouch, A., and G. O. M. Moustafa, 2020. Synthesis, spectroscopic characterization and antimicrobial studies of Mn (II), Co (II), Ni (II), Cr (III) and Fe (III) melatonin drug complexes. Egyptian Journal of Chemistry, 63 (4): 1469-1481. https://doi.org/10.21608/ejchem.2020.21193.2263

Alwash, B. M. J., Saed, N. H. J., and ALqusay, S. A. 2017. The effect of alcoholic extract Eriobotrya japonica leaves on some bacterial genes and some chemical and microbiological properties of beef. The Iraqi Journal of Agricultural Science, 48(5):1231-1238.‏ https://doi.org/10.36103/ijas.v48i5.332

Andres, S., L. Huerga, J. Mateo, M. L. Tejido, R. Bodas and L. Moran 2014. The effect of quercetin dietary supplementation on meat oxidation processes and texture of fattening lambs. Meat Science, 96,806–811. https://doi.org/10.1016/j.meatsci.2013.09.020

Al-Sudani, H. M. Khalaf, and A.M. S. Al-Rubeii. 2021. The effect of nano bentonite supplementation in some carcass characteristics of awassi lambs." Systematic Reviews in Pharmacy 12(1):1103-1113. Print. doi:10.31838/srp.2021.1.153

Araúj M. K., A. M. Gumiela, K Bordin,., F. B. Luciano and R. E. F. de Macedo. 2018. Combination of garlic essential oil, allyl isothiocyanate, and nisin Z as bio-preservatives in fresh sausage. Meat Science, 143, 177-183. http://dx.doi.org/10.1016/j.meatsci.2018.05.002

Arihara, K. 2004. Functional foods. In W. K. Jensen, C. Devine, and M. Dikeman (Eds.), Encyclopedia of Meat Sciences pp:492−499. Oxford: Elsevier

Arihara, K. 2006. Strategies for designing novel functional meat products. Meat Science, 74, 219−229.

Baker, I. A., and J. E. Alkass, 2020. A comparative study on the effectiveness of adding rosemary and ginger extracts on meat quality during freezing storage. Iraqi Journal of Agricultural Sciences, 51(1):333-338.‏ https://doi.org/10.24126/jobrc.2023.17.1.693

Bauer A.W, D.M. Perry, W.M.M. Kirby. 1959. Single-disk antibiotic-sensitivity testing of staphylococci. AMA Arch Intern Med 1959;104:208. https://doi.org/10.1001/archinte.1959.00270080034004

Alfawaz, M., J. S. Smith, and I. J. Jeon. 1994. Maillard reaction products as antioxidants in pre-cooked ground beef. Food Chemistry, 51(3):311-318.‏ https://doi.org/10.1016/0308 8146(94)90032-9

Daniela F. O., B. Ruth, C. Gladys, C. Fernanda, M. Nora 2013. Kinetics of color and texture changes of beef during stor age J Food Sci Technol. 50 (4): 821-825’. doi: 10.1007/s13197-012-0885-7

Di Bernardini, R., P. Harnedy, D. Bolton, J. Kerry, E. O’Neill, A. M. Mullen and M. Hayes (2011). Antioxidant and antimicrobial peptidic hydrolysates from muscle protein sources and by-products. Food Chemistry, 124(4): 1296-1307. https://doi.org/10.1016/j.foodchem.2010.07.004

Duncan,B.D. 1955. Multiple range and multiple F test .Biometric.11:1-24.

Elmer, W. K, D. A. Stephen, V.R. Dowell, Jr. Herbert,.and M. Sommer. 1979. Color Atlas and text book of Diagnetic Microbiology.Priinted in the united starter of America.

Hać-Szymańczuk, E., E. Lipińska, and M. Stasiuk. 2011. ‘The effect of rosemary preparations on the microbial quality and TBARS value of model pork batters’, Acta Scientiarum Polonorum Technologia Alimentaria, 10(2):165–174.


Harrigan, W. F. and M. E. McCance.1976. Laboratory Methods in Food and Dairy Microbiology. Academic Press, London. DOI: 10.4236/ce.2016.714189

Hussein, S. I.; A. F. ,Kaluf; Y. Ahmed ;B. Ahmed; and A.Iyad .2020. Determination of inhibition activity of α- amylase enzyme, antioxidant activity, antibacterial activity and phenolic compounds by using some medical plants. Iraqi Journal of Agricultural Sciences:51(1):411-421. https://doi.org/10.36103/ijas.v51i1.940

Khalef, A. A. 2017. ‘Effect of pomegranate peel extract on some physical, microbial and sensory properties of birker made from frozen chicken meat. Tikrit University Journal of Agricultural Sciences. Special Issue of Proceedings of the Sixth Scientific Conference of Agricultu’,1813–1646. DOI: 10.1088/1755-1315/788-6

McCarthy, T. L., , J. P. Kerry, J. F. Kerry, P. B. Lynch, and D. J. Buckley. 2001. Evaluation of the antioxidant potential of natural food/plant extracts as compared with synthetic antioxidants and vitamin E in raw and cooked pork patties. Meat Science, 57:45–52. DOI:10.1080/19476330903572945

Mcclean, Stephen; BEGGS, B. Louise Welch, W. Robert. 2014. Antimicrobial activity of antihypertensive food-derived peptides and selected alanine analogues. Food Chemistry. 146: 443-447.


Nadalian, M., N. Kamaruzaman, M. S. M. Yusop, A. S. Babji and S. M. Yusop. 2019. Isolation, purification and characterization of antioxidative bioactive elastin peptides from poultry skin. Food Science of Animal Resources, 39(6):966.‏ DIO:10.5851/kosfa.2019.e90

Naveena, B. M., Vaithiyanathan, S., Muthukumar, M., A. R. Sen, Y. P. Kumar, M. Kiran, and K. R. Chandran. 2013. Relationship between the solubility, dosage and antioxidant capacity of carnosic acid in raw and cooked ground buffalo meat patties and chicken patties. Meat Science, 95(2): 195-202.https://doi.org/10.1016/j.meatsci.2013.04.043

Patel S. 2015. Functional food relevance of whey protein: A review of recent findings and scopes ahead. J. Funct. Foods:19(A):308-1936. https://doi.org/10.1016/j.jff.2015.09.040

Pereira, A. M., A. D. Costa, S. C. Dias, M. Casal and R. Machado. 2021. Production and purification of two bioactive antimicrobial peptides using a two-step approach involving an elastin-like fusion tag. Pharmaceuticals, 14(10):956.https://doi.org/10.3390/ph14100956

Quintieri, L., L. Caputo, L. Monaci, D. Deserio, M. Morea and F. Baruzzi. 2012. Antimicrobial efficacy of pepsin-digested bovine lactoferrin on spoilage bacteria contaminating traditional Mozzarella Cheese. Food Microbiology, 31(1):64-71. https://doi.org/10.1016/j.fm.2012.02.015

Roberts, D. and M. Greenwood 2003. Practical food microbiology, 3ed. Blackwell Publishing Ltd Blackwell Publishing Inc., 350 Main Street, Malden, Massachusetts 02148-5018, USA. DOI:10.1002/9780470757512

Saiga, A., T. Okumura, T. Makihara, S. Katsuta, T. Shimizu, R. Yamada. 2003. Angiotensin I-converting enzymes inhibitory peptides in a hydrolyzed chicken breast muscle extract. Journal of Agricultural and Food Chemistry, 51: 174−1745.


Saleh, A. M., and G. M. Mohammed. 2020. Studying the effect of adding different concentrations of Ataxanthin and allyl isothiocyanate and their synergistic action in lipid oxidation and some quality characteristics for minced veal meat at cold storage. Diyala Agricultural Sciences Journal, 12(special Issue): 664-674.


Salman, Z. O., B. M. J. Alwash, and E. J.Kadhim. 2019. Effect of essential oil of Cestrum nocturnum flowers cultivated in Iraq as antioxidant and elongation cold storage period of minced meat. Iraqi Journal of Agricultural Sciences, 50(2); 601-607. https://doi.org/10.36103/ijas.v2i50.659

Szmańko, T., Lesiów, T., and J. Górecka, 2021. The water-holding capacity of meat: A reference analytical method. Food Chemistry, 357, 129727.‏ https://doi.org/10.1016/j.foodchem.2021.129727

Sánchez, A., and A. Vázquez 2017. Bioactive peptides: A review. Food Quality and Safety, 1(1): 29-46. https://doi.org/10.1093/fqsafe/fyx006

Seligman, M., R. G., Eilberg and L. Fishman. 1975. Mineralization of elastin extracted from human aortic tissues. Calcified tissue research, 17:229-234 https://doi.org/10.1007/BF02546686

Singh, P. K., G. Jairath and S. S. Ahlawat. 2016. Nanotechnology: a future tool to improve quality and safety in meat industry. Journal of Food Science and Technology, 53(4): 1739-1749. https://doi.org/10.1007/s13197-015-2090-y

Taghipour, M. J., H. Ezzatpanah, and M. Ghahderijani. 2023. In vitro and in silico studies for the identification of anti-cancer and antibacterial peptides from camel milk protein hydrolysates. Plos one, 18(7): e0288260.‏ https://doi.org/10.1371/journal.pone.0288260

Théolier J, R. Hammami, P. Labelle, I. Fliss and J.Jean .2013. Isolation and identification of antimicrobial peptides derived by peptic cleavage of whey protein isolate. J Funct Foods.:5(2):706–14.


Vercruysse, L., J. van Camp and G. J. Smagghe. 2005. ACE Inhibitory peptides derived from enzymatic hydrolysates of animal muscle protein: A review. Journal of Agricultural and Food Chemistry, 53, 8106−8115.https://doi.org/10.1021/jf0508908

Wafaa, N., H. A., E. M. A. Elbarbary , H. A. Ibrahim, Mohamed and H. Jenssen. 2022. Effect of enzyme type and hydrolysis time on antibacterial and antioxidant activity of whey protein hydrolysates. Iraqi Journal of Agricultural Sciences, 53(6):1340-1357.‏ https://doi.org/10.36103/ijas.v53i6.165

Zainy, Z. I., and A. M. S. Alrubeii. 2023. Effect of replacement nitrite by beetroot and silybum marianum powder in physical characteristics and lipid oxidation for pasterrma. Iraqi Journal of Agricultural Sciences, 54(4):1131-1136. https://doi.org/10.36103/ijas.v54i4.1806

Zangana, B. S. R., andJ. S. H , Al-Shamery. 2016. Effect of partial replacement of some plant sources in quality and sensory characteristics of processed of goose meat burger. Iraqi Journal of Agricultural Sciences, 47(4):1089-1100.‏ https://doi.org/10.36103/ijas.v47i4.548

Zarzosa-Mreno, D., C. Avalos-Gómez, L. S. Ramírez-Texcalco, E. Torres-López, R. Ramírez-Mondragón, - J. O Hernández. Ramírez, and de la M. Garza, 2020. Lactoferrin and its derived peptides: an alternative for combating virulence mechanisms developed by pathogens. Molecules, 25(24): 5763. https://Doi:10.3390/molecules25245763.







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