CYTOTOXIC EFFECT OF PLASMA ACTIVATED MEDIUM ON THETREATED BREAST CANCER
DOI:
https://doi.org/10.36103/bm6h2e50Keywords:
plasma jet, caspases-9,TNF-α, and apoptosisAbstract
This study was aimed to evaluate the effect of plasma activated medium (PAM) on breast cancer cell lines (MCF7 and AMJ13) and compared it to non malignant one (ASCs). The cytotoxic effect of 10,15,20, and 25 min prepared PAM was measured and the results indicated that all cells were influenced in the exposure times (20 and 25)min, especially after 48 hours of incubation with lesser influence on normal cells. Based on the results 20 min was determined as the effective dose on 50% of cancer cells with minimal influence on normal one. Further, tumor necrosis factor-alpha (TNF-α) was assessed and the results indicated reduction in the level of this cytokine after PAM treatment, MCF7 showed significant 2reduction in both incubation intervals while AMJ13 and ASCs significantly varied only after 48hrs.Finally, caspase-9was analyzed to identify the apoptosis route and the obtained data revealed that both MCF7 and AMJ13raisedsignificantlyafter 24 and 48hrs.In contrast, ASC cells revealed non-significant changes in both intervals.
References
Abdulhasan, G. A. 2021. Lemongrass essential oil shows an antiproliferative effect on lung cancer cells and its role in expression of p53 gene. Iraqi Journal of Agricultural Sciences, 52(2), 359-366. https://doi.org/10.36103/ijas.v52i2.1297
Abdullah, W. K., and F. A. Maha, 2022. Caspase 9 gene expression and caspase 9 protein in chronic myeloid leukemia in Iraq. Iraqi Journal of Agricultural Sciences, 53(5), 994-1002. https://doi.org/10.36103/ijas.v53i5.1613
Al-Aamri, H. M., H. R. Irving, C. Bradley, and T.Meehan-Andrews. 2021. Intrinsic and extrinsic apoptosis responses in leukaemia cells following daunorubicin treatment. BMC cancer, 21(1): 1-10
Al-Attaby, A.K.T. and M.Q.D.Al-Lami. 2019. Role of calcium-regulating hormones, adipocytokines and renal function test in the progress of type 2 diabetes mellitus in a sample of Iraqi patients. The Iraqi Journal of Agricultural Science, 50(1), pp.343-351
An, L., X.Dou, M. Wang, W. Luo, Q.Ma, and X. Liu. 2020. Involvement of TNF-alpha and IL-10 in breast cancer and patient survival. Tropical Journal of Pharmaceutical Research, 19(10): 2033-2039
Ayeda, M. M., and J. M. Awda, 2023. Cytotoxic activity of basil seeds (Ocimum baslicum L) extracts on some breast cancer cell lines (in vitro). Iraqi Journal of Agricultural Sciences, 54(4), 928-938. https://doi.org/10.36103/ijas.v54i4.1782
Berberoglu, U., E.Yildirim, andO.Celen. 2004. Serum levels of tumor necrosis factor alpha correlate with response to neoadjuvant chemotherapy in locally advanced breast cancer. The International journal of biological markers, 19(2):130-134
Blackert, S., B. Haertel, K.Wende, T.von Woedtke, and U.Lindequist. 2013. Influence of non-thermal atmospheric pressure plasma on cellular structures and processes in human keratinocytes (HaCaT). Journal of dermatological science, 70(3): 173-181
Brentnall, M., L. Rodriguez-Menocal, , R.L.De Guevara, E.Cepero, and L.H.Boise. 2013. Caspase-9, caspase-3 and caspase-7 have distinct roles during intrinsic apoptosis. BMC cell biology, 14(1):1-9
Cheng, X., K. Rajjoub, A. Shashurin, D. Yan, J. H. Sherman, K.Bian, F.Murad, and M. Keidar. 2017. Enhancing cold atmospheric plasma treatment of cancer cells by static magnetic field. Bioelectromagnetics, 38(1): 53-62
Harley, J. C., N. Suchowerska, and D. R.McKenzie. 2020. Cancer treatment with gas plasma and with gas plasma-activated liquid: positives, potentials and problems of clinical translation. Biophysical reviews, 12(4): 989–1006.
Hashim, S. T. and M. M. Nema. 2018. Production and purification of chitinase from Aeromonas spp. And study its effect on some cancer cell lines in vitro. The Iraqi Journal of Agricultural Science, 49(3):438-444
Jo, A., H. M. Joh, T. H. Chungand J. W. Chung. 2020. Anticancer effects of plasma-activated medium produced by a microwave-excited atmospheric pressure argon plasma jet. Oxidative medicine and cellular longevity, 4205640
Kadhim, R. I., and E. D. Salman 2022. Genetic variability in the prncr1 gene among a sample of iraqi prostate cancer patients. Iraqi Journal of Agricultural Sciences, 53(3), 515-521. https://doi.org/10.36103/ijas.v53i3.1559
Keidar, M., A.Shashurin, O.Volotskova, M. Ann Stepp, P.Srinivasan, A.Sandler, and B. Trink. 2013. Cold atmospheric plasma in cancer therapy. Physics of Plasmas, 20(5): 057101
Kras, K. M., D. B.Hausman, and R. J. Martin. 2000. Tumor necrosis factor‐α stimulates cell proliferation in adipose tissue‐derived stromal‐vascular cell culture: promotion of adipose tissue expansion by paracrine growth factors. Obesity Research, 8(2): 186-193
Laroussi, M. 2020. Cold Plasma in medicine and healthcare: The new frontier in low temperature plasma applications. Frontiers in Physics, 8:74
Ly, L., X. Cheng, S. R.Murthy, T.Zhuang, O.Z.Jones, G.Basadonna, M.Keidar, and J. Canady. 2020. Canady cold plasma conversion system treatment: An effective inhibitor of cell viability in breast cancer molecular subtypes. Clinical Plasma Medicine, 19:100109
Maisch T., A. K. Bosserhoff, P. Unger, J. Heider, T. Shimizu, J. L. Zimmermann, G. E. Morfill, M. Landthaler, S. Karrer. 2017. Investigation of toxicity and mutagenicity of cold atmospheric argon plasma. Environ. Mol. Mutagen. 58:172–177
Mercogliano, M. F., S. Bruni, F. Mauro, P. V. Elizalde, and, R. Schillaci. 2021. Harnessing tumor necrosis factor alpha to achieve effective cancer immunotherapy. Cancers 2021, 13, 564
Mohamed, H., R.Clemen, E.Freund, J.W. Lackmann, K.Wende, J.Connors, E.K.Haddad, W.Dampier,B.Wigdahl, V.Miller, and S.Bekeschus. 2021. Non-thermal plasma modulates cellular markers associated with immunogenicity in a model of latent HIV-1 infection. PloS one, 16(3): 0247125
NA, A. 2023. Phenolic compounds content, antioxidant, antibacterial and antifungal activities of red onions skin. Iraqi Journal of Agricultural Sciences, 54(4):1050-1057. https://doi.org/10.36103/ijas.v54i4.1794
Olsson, M., and B.Zhivotovsky. 2011. Caspases and cancer. Cell death and differentiation, 18(9): 1441–1449
Omar, S.M. and B.M. Mohammed. 2020. Antigenotoxic effects of melatonin against chromosome damage induced by 7, 12-dymethylbenz (a) anthracene. The Iraqi Journal of Agricultural Science, 51(3): 916-923
Sersenová, D., Z.Machala, V.Repiská, and H.Gbelcová. 2021. Selective apoptotic effect of plasma activated liquids on human cancer cell lines. Molecules (Basel, Switzerland), 26(14): 4254
Tanaka, H., M.Mizuno, Y.Katsumata, K. Ishikawa, H.Kondo, H.Hashizume, Y.Okazaki, , S.ToyokuniK.Nakamura, N.Yoshikawa, and H.Kajiyama. 2019. Oxidative stress-dependent and-independent death of glioblastoma cells induced by non-thermal plasma-exposed solutions. Scientific Reports, 9(1): 1-12
Tang, D., R. Kang, T. V. Berghe, P. Vandenabeele, and G.Kroemer. 2019. The molecular machinery of regulated cell death. Cell research, 29(5): 347-364
Terefinko, D., A. Dzimitrowicz, A.Bielawska-Pohl, A.Klimczak, P. Pohl, and P.Jamroz. 2021. The influence of cold atmospheric pressure plasma-treated media on the cell viability, motility, and induction of apoptosis in human non-metastatic (MCF7) and metastatic (MDA-MB-231) breast cancer cell lines. International Journal of Molecular Sciences, 22(8): 3855
Wang, P., R. Zhou, P. Thomas, L. Zhao, R. Zhou, S. Mandal, M. K. Jolly, D. J. Richard, B. Rehm, K. Ostrikov, K., Dai, E. D. Williams, and E. W.Thompson, 2021. Epithelial-to-mesenchymaltransition enhances cancer cell sensitivity to cytotoxic effects of cold atmospheric plasmas in breast and bladder cancer systems. Cancers, 13(12): 2889.
Wang, Q., L. X. Zhang, Y. Yuan, X. Ou, Z. Zhu, and L. Zhang. 2016. The relationship between the Bcl-2/Bax proteins and the mitochondria-mediated apoptosis pathway in the differentiation of adipose-derived stromal cells into neurons. PloS one, 11(10): e0163327
Xiang, L., X.Xu, S.Zhang, D.Cai, and X. Dai.2018. Cold atmospheric plasma conveys selectivity on triple negative breast cancer cells both in vitro and in vivo. Free Radical Biology and Medicine, 124: 205-213
Xu, D., Y. Xu, Q.Cui, D.Liu, Z.Liu, X. Wang, Y.Yang, M.Feng, R.Liang, H. Chen, and K.Ye. 2018. Cold atmospheric plasma as a potential tool for multiple myeloma treatment. Oncotarget, 9(26): 18002
Yan, D., A. Malyavko, Q. Wang, K. K. Ostrikov, J. H.Sherman, and M.Keidar. 2021. Multi-modal biological destruction by cold atmospheric plasma: capability and mechanism. Biomedicines, 9(9): 1259
Yang, X., C. Yang, L.Wang, Z.Cao, Y.Wang, C. Cheng, and Y.Zhao. 2020. Inhibition of basal cell carcinoma cells by cold atmospheric plasma activated solution
and differential gene expression analysis. International Journal of Oncolo-gy, 56(5): 1262-1273
Zubor, P., Y.Wang, A.Liskova, M.Samec, L.Koklesova, Z.Dankova, A.Dørum, K.Kajo, D.Dvorska, V.Lucansky, B.Malicherova, I.Kasubova, J.Bujnak, M.Mlyncek, C. A.Dussan, P.Kubatka, D.Büsselberg, and O.Golubnitschaja. 2020. Cold atmospheric pressure plasma (CAP) as a new tool for the management of vulva cancer and vulvar premalignant lesions in gynaecologic-aloncology. International journal of molecular sciences, 21(21): 7988.
Downloads
Published
Issue
Section
License
Copyright (c) 2024 IRAQI JOURNAL OF AGRICULTURAL SCIENCES
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
How to Cite
2.jpg)
2.jpg)
