DETECTION OF GENES RESPONSIBLE FOR HEAVY METALS RESISTANCE IN LOCALLY ISOLATED PSEUDOMONAS SPP.

Authors

  • Al-Sajad M. S.
  • H.A.A. Alsalim

DOI:

https://doi.org/10.36103/wgz9vb91

Keywords:

plant growth promoting rhizobacteria (PGPR), P. aeruginosa, copA, copB and czcA.

Abstract

Plant growth-promoting rhizobacteria (PGPR) that can tolerate heavy metals, provide the basis for microbial inoculums showing heavy metals tolerance properties. This study was aimed to detect the heavy metal resistance genes in plant-growth-promoting Pseudomonas spp. isolated from many agricultural fields. The collected isolates were screened for their plant growth-promoting (PGP) traits, hydrolytic enzymes, Siderophore, ammonia, and indole-3-acetic acid (IAA). Then, subjected to concentrations of CuSO4, CdCl2, and ZnCl2 to determine the minimum inhibitory concentration (MIC). The DNA was extracted from the selected isolates then PCR test was achieved to detect copA, copB, and czcA genes, responsible for heavy metal resistance. Seventy Pseudomonas spp. isolates were obtained; 41 (58.57%), 6 (8.57%), and 15 (21.42%) isolate produced protease, cellulase, and pectinase, respectively. The isolates were positive for siderophore and ammonia production. However, 68 (97.14%) isolates have produced indole-3-acetic acid. Eight isolates were selected and identified as Pseudomonas aeruginosa using the Vitek 2 compact system. The isolates' resistance to heavy metals differed significantly. The isolate B49 had a higher resistance to CuSO4 (MIC = 3200 µg/ml) and ZnCl2 (MIC = 2600 µg/ml), while the isolate B66 recorded a higher resistance to CdCl2 (MIC = 1000 µg/ml).   copB, and czcA genes were detected in the eight P. aeruginosa isolates, while copA gene was detected in seven, except B69.

References

Adekanmbi, A. O., O. O. Adelowo, A. I. Okoh, and O. E. Fagade. 2019. Metal-resistance encoding gene-fingerprints in some bacteria isolated from wastewaters of selected printeries in Ibadan, South-western Nigeria. Journal of Taibah University for Science, 13(1): 266-273.

Agrawal, T., A. S. Kotasthane, A. Kosharia, , R. Kushwah, N. W. Zaidi, and U. S. Singh. 2017. Crop specific plant growth promoting effects of ACCd enzyme and siderophore producing and cynogenic fluorescent Pseudomonas. 3 Biotech, 7(1): 27.

Ahemad, M. 2019. Remediation of metalliferous soils through the heavy metal resistant plant growth promoting bacteria: paradigms and prospects. Arabian Journal of Chemistry, 12(7): 1365-1377.

Akinbowale, O. L., H. Peng, P. Grant, and M. D. Barton. 2007. Antibiotic and heavy metal resistance in motile aeromonads and pseudomonads from rainbow trout (Oncorhynchus mykiss) farms in Australia. International Journal of Antimicrobial Agents, 30(2): 177-182.

Akter, S., J. Kadir, A. S. Juraimi and H. M. Saud. 2016. In vitro evaluation of Pseudomonas bacterial isolates from rice phylloplane for biocontrol of Rhizoctonia solani and plant growth promoting traits. In Journal of Environmental Biology, 37(4): 597.

Al-Aboudi, H. J., Al-Rudainy, A. J. and A. A. M. Maktoof. 2022. Accumulation of lead and cadmium in tissue of cyprinus carpio collected from cages of Al-gharraf river/Thi qar /Iraq. Iraqi Journal of Agricultural Sciences, 53(4), 819-824.‏ https://doi.org/10.36103/ijas.v53i4.1594

Ali, S., S. Hameed, M. Shahid, M. Iqbal, G. Lazarovits and A Imran. 2020. Functional characterization of potential PGPR exhibiting broad-spectrum antifungal activity. Microbiological Research, 232, 126389.

Alsalim, H.A.A. 2020. Isolation and characterization of phosphate solubilizing Pseudomonas species and assess its efficacy as plant growth promoter. Biochemical and Cellular Archives, 20, 2301-2308.

Aljuboori, F. K., Ibrahim, B. Y., and A. H. Mohamed. 2022. Biological control of the complex disease of Rhizoctonia solani and Root-Knot nematode Meloidogyne javanica on chickpea by Glomus spp. and Pseudomonas sp. Iraqi Journal of Agricultural Sciences, 53(3): 669-676.‏ https://doi.org/10.36103/ijas.v53i3.1577

Al-Salmany, S. W. K. and I. A. Ibrahim. 2021. Phytoextraction of cadmium and lead from a contaminated soils using eucalyptus seeding. Iraqi Journal of Agricultural Sciences, 52(4):‏ 827–810 https://doi.org/10.36103/ijas.v52i4.1390

Benhalima, L., S. Amri, M. Bensouilah and R. Ouzrout. 2020. Heavy metal resistance and metallothionein induction in bacteria isolated from Seybouse river, Algeria. Appl Ecol Environ Res, 18(1): 1721-1737.

Chaiharn, M. and S. Lumyong. 2011. Screening and optimization of indole-3-acetic acid production and phosphate solubilization from rhizobacteria aimed at improving plant growth. Current Microbiology, 62(1): 173-181.

Chopra, A., S. Bobate, P. Rahi, A. Banpurkar, P. B. Mazumder and S. Satpute. 2020. Pseudomonas aeruginosa RTE4: A Tea Rhizobacterium With Potential for Plant Growth Promotion and Biosurfactant Production. In Frontiers in Bioengineering and Biotechnology, 8: 861.

David, B. V., G. Chandrasehar and P. N. Selvam. 2018. Pseudomonas fluorescens: a plant-growth-promoting rhizobacterium (PGPR) with potential role in biocontrol of pests of crops. In Crop improvement through microbial biotechnology. pp: 221-243. Elsevier.‏

Folasade, M. O. and O. A. Joshua. 2008. Some properties of extracellular protease from Bacillus licheniformis Lbb1-11 isolated from ‘iru’, A traditionally fermented African locust bean condiment. J. Biotechnol. Biochem. 3: 42-46.

Gallardo-Benavente, C., J. L. Campo-Giraldo, J. Castro-Severyn, A. Quiroz and J. M. Pérez-Donoso. 2021. Genomics Insights into Pseudomonas sp. CG01: An Antarctic Cadmium-Resistant Strain Capable of Biosynthesizing CdS Nanoparticles Using Methionine as S-Source. Genes, 12(2): 187.‏

Ghaima, K. K., A. I. Mohamed, W. Y. Al Meshhdany and A. A. Abdulhassan. 2017. Resistance and bioadsorption of cadmium by Pseudomonas aeruginosa isolated from agricultural soil. International Journal of Applied Environmental Sciences, 12(9): 1649-1660.‏

Holt, J.C.; N.R. Krieg, 1984. Bergeys’ Manual of Systemic Bacteriology .4th (ed). William and Willkins, Baltimor. London. 9: 40-97.

Ibrahim, A. M., R. A. Hamouda, N. E.-A. El-Naggar and F. M. Al-Shakankery. 2021. Bioprocess development for enhanced endoglucanase production by newly isolated bacteria, purification, characterization and in-vitro efficacy as anti-biofilm of Pseudomonas aeruginosa. Scientific Reports, 11(1): 1-24.

Ikhwan, A. and A. I. Putra. 2021. Industrial sludge active bacteria potency test of PT surabaya industrial estate rungkut (SIER) as a heavy metal bioremediator and biofertilizer. IOP conference series: Earth and Environmental Science, 752(1): 012005. 21. Imron, M. F., S. B. Kurniawan and S. R. S. Abdullah. 2021. Resistance of bacteria isolated from leachate to heavy metals and the removal of Hg by Pseudomonas aeruginosa strain FZ-2 at different salinity levels in a batch biosorption system. Sustainable Environment Research, 31(1): 1-13.‏

Jadhav, H. P., S. S. Shaikh and R. Z. Sayyed. 2017. Role of Hydrolytic Enzymes of Rhizoflora in Biocontrol of fungal Phytopathogens: an Overview. Rhizotrophs: Plant growth promotion to Bioremediation, pp:183-203.‏

Jeanvoine, A., A. Meunier, H. Puja, X. Bertrand, B. Valot and D Hocquet. 2019. Contamination of a hospital plumbing system by persister cells of a copper-tolerant high-risk clone of Pseudomonas aeruginosa. Water Research, 157: 579-586.

Kotasthane, A. S., T. Agrawal, N. W. Zaidi and U. S. Singh. 2017. Identification of siderophore producing and cynogenic fluorescent Pseudomonas and a simple confrontation assay to identify potential bio-control agent for collar rot of chickpea. 3 Biotech, 7(2): 1-8.

Li, K., R. R. Pidatala and W. Ramakrishna. 2012. Mutational, proteomic and metabolomic analysis of a plant growth promoting copper-resistant Pseudomonas spp. FEMS Microbiology Letters, 335(2): 140-148.

Malik, A., and A Aleem. 2011. Incidence of metal and antibiotic resistance in Pseudomonas spp. from the river water, agricultural soil irrigated with wastewater and groundwater. Environmental Monitoring and Assessment, 178(1): 293-308.

Martins, V. V., M. O. B. Zanetti, A. Pitondo-Silva and E. G. Stehling. 2014. Aquatic environments polluted with antibiotics and heavy metals: a human health hazard. Environmental Science and Pollution Research, 21(9): 5873-5878.

Marzban, A., G. Ebrahimipour, M. Karkhane, S. Mohseni, A. Moradi and H. Alaee. 2011. Study of heavy metal and antibiotic resistance on a Pseudomonas aeruginosa strain isolated from soils contaminated with coal tar. International Journal of Biotechnology and Biochemistry, 7(4): 451-456.

Nanda, M., V. Kumar and D. K Sharma. 2019. Multimetal tolerance mechanisms in bacteria: The resistance strategies acquired by bacteria that can be exploited to ‘clean-up’heavy metal contaminants from water. Aquatic toxicology, 212: 1-10.‏

Parasuraman, P., S. S. Pattnaik, S. Busi, N. Marraiki, A. M. Elgorban and A. Syed. 2020. Isolation and characterization of plant growth promoting rhizobacteria and their biocontrol efficacy against phytopathogens of tomato (Solanum lycopersicum L.). Plant Biosystems-An International Journal Dealing with all Aspects of Plant Biology, 1-7.‏

Payne, S.M. 1980. Synthesis and utilization of siderophores by Shigella flexneri. Journal of Bacteriol., 143(3): 1420-1424.

Pereira, S. I. A., L. Barbosa and P. M. L. Castro. 2015. Rhizobacteria isolated from a metal-polluted area enhance plant growth in zinc and cadmium-contaminated soil. International Journal of Environmental Science and Technology, 12(7): 2127-2142.

Pitondo‐Silva, A., G. B. Gonçalves and E. G. Stehling. 2016. Heavy metal resistance and virulence profile in Pseudomonas aeruginosa isolated from Brazilian soils. Apmis, 124(8): 681-688.

Rana, S., Sharma, R., and M. Kaur. 2020. Biocontrol potentialities of native Pseudomonas isolates of Himachal Pradesh against plant pathogenic fungi Dematophora sp., Fusarium sp., Pythium sp. and Sclerotium sp. of apple Rhizosphere. 9(5): 1079-1083.

Roosa, S., C. Vander Wauven, G. Billon, S. Matthijs, R. Wattiez and D. C. Gillan. 2014. The Pseudomonas community in metal-contaminated sediments as revealed by quantitative PCR: a link with metal bioavailability. Research in Microbiology, 165(8): 647-656.

SAS. 2012. Statistical Analysis System, User's Guide. Statistical. Version 9.1th ed. SAS. Inst. Inc. Cary. N.C. USA.

‏37. Sandilya, S. P., P. M. Bhuyan, V. Nageshappa, D. K. Gogoi and D. Kardong. 2017. Impact of Pseudomonas aeruginosa MAJ PIA03 affecting the growth and phytonutrient production of castor, a primary host-plant of Samia ricini. Journal of Soil Science and Plant Nutrition, 17(2): 499-514.

Al-Hilfy, I. H. H. 2022. Effect of brassinolide on some growth traits and biological yield of bread wheat. Iraqi Journal of Agricultural Sciences, 53(2): 322-328.‏ https://doi.org/10.36103/ijas.v53i2.1539

Suresh, P., S. Vellasamy, K. S. Almaary, T. M. Dawoud and Y. B. Elbadawi. 2021. Fluorescent pseudomonads (FPs) as a potential biocontrol and plant growth promoting agent associated with tomato rhizosphere. Journal of King Saud University - Science, 33(4): 101423.

Wadekar, S. V., and S. R. Kagne. 2020. Potential plant growth-promoting properties of Pseudomonas spp. isolated from the Rhizosphere of the Soybean Plant. Agbir, 36(4): 60-62.

Wallace, R. L., D. L. Hirkala and L. M. Nelson. 2017. Postharvest biological control of blue mold of apple by Pseudomonas fluorescens during commercial storage and potential modes of action. Postharvest Biology and Technology, 133: 1-11.

Yasin, B. O. O. Ali and T. S Rashid. 2021. Antagonistic activity and plant growth promoting rhizobacteria isolated from forest plant rhizosphere against Fusarium solani on thuja seedlings. Iraqi Journal of Agricultural Sciences, 52(6): 1508-1515. https://doi.org/10.36103/ijas.v52i6.1492

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2024-02-25

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How to Cite

Al-Sajad M. S., & H.A.A. Alsalim. (2024). DETECTION OF GENES RESPONSIBLE FOR HEAVY METALS RESISTANCE IN LOCALLY ISOLATED PSEUDOMONAS SPP . IRAQI JOURNAL OF AGRICULTURAL SCIENCES, 55(1), 361-370. https://doi.org/10.36103/wgz9vb91

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