ISOLATION AND IDENTIFICATION OF BIOFILM PRODUCING ENTEROCOCCUS FAECALIS FROM ROOT CANAL
DOI:
https://doi.org/10.36103/ijas.v55iSpecial.1910Keywords:
bacterial infections, Polymerase chain reaction, teeth, antibiotic sensitivity.Abstract
This study was aimed to one of the most prevalent causes for endodontic treatment failure is the presence of Enterococcus faecalis bacterium within teeth root canals. To achieve successful treatment, it is so important to study E. faecalis behavior. The aim of study was to investigate biofilm production and antibiotic sensitivity of E. faecalis isolated from root canals. Results showed isolation of E. feacalis (65%) of samples, identified by specific gene by PCR technique. Most isolates were sensitive to Imipenem and resistant to Erythromycin, Clindamycin, Tetracycline and Trimethoprim. Strong biofilm production was detected among 29.5% of highest antibiotic resistant isolates. The results may indicate that infected root canals with E. feacalis may lead to serious complication for patients.
References
Akbari A. M., B. M. H. Soroush, M. Aghazadeh, F. Jafari, H. Beomide and M. Haghdoost. 2017. Virulence genes in biofilm producer Enterococcus faecalis isolates from root canal infections. Cell Molecular Biology, 63(5):55–59.
AL-Azzawi, H. A. M. 2014. Detection of Vancomycin-Resistance Among Methicillin –Resistance Staphylococcus Aureus and Their Effect on Autolysis. M.Sc. Thesis College of Science Baghdad University. pp: 98-99.
Al-Saadi F. S. 2007. A Study of Bacterial Resistance Causing Urinary Tract Infections for Some Antibiotics and their Production of β-lactamase Enzymes. M.Sc. Thesis College of Science Mustansiriya University. pp: 75-76.
Al-Ta’i, K. L. Sh. 2013. The Role of Partially Purified Lipocic Acid from E. faecalis Locally Isolated from Different Clinical Sources in Adhesion. M.Sc. Thesis College of Science. Baghdad University. pp: 72-77.
Brooks G.F., J.S. Butel and S.A. Morse. 2004. Medical Microbiology.
Butler, E., R. F. Oien, C. Lindholm, T. C.Olofsson, B. Nilson and A. Vasquez. 2014. A pilot study investigating lactic acid bacterial symbionts from the honeybee in inhibiting human chronic wound pathogens. Int Wound J. 13 (5): 729–737. Cell Molecular Biology, 121:1890-1897.
Ch’ng J., K.K.L. Chong, L.N. Lam, J.J. Wong and K.A. Kline. 2019. Biofilm-associated infection by enterococci. Nat Rev Microbiol,17, 82-94.
Clinical and Laboratory Standards Institute(CLSI). 2020. Performance Standards for Antimicrobial Susceptibility Testing. Approved Standard. CLSI Document M100. pp: 106-108.
Cogulu, D., A. Uzel , O. Oncag, S. D. Aksoy and C. Eronat. 2007. Detection of Enterococcus faecalis in necrotic teeth root canals by culture and polymerase chain reaction methods. European Journal of Dentistry, 01(04): 216–221.
Collee, J.G., A. G. Fraser, B. P. Marmison, and S. A. Simmon. 1996. Mackie and Macathneys. Practical Medical Microbiology. 14th ed, Churchill Livingstone, U.K. pp: 131-151.
Costerton J.W., L. Montanaro and C.R. Arciola. 2005. Biofilm in implant infections: its production and regulation. Int J Artif Organs 28: 1062-1068.
Diab, M., D. Salem and A. El-Shenawy. 2019. Detection of high level aminoglycoside resistance genes among clinical isolates of Enterococcus Species. Egypt J Med Hum Genet. Pp: 20, 28.
Dupre, I., S. Zanetti, A. M. Schito, G. Fadda and L. A. Sechi. 2003. Incidence of virulence determinants in clinical Enterococcus faecium and Enterococcus faecalis isolates collected in Sardinia (Italy). J. Med. Microbiol., 52: 491-498.
Fatemeh S. H. Hajar, K. Saeed, M. Golshan, A. D. Aram and F. S. Ahmad. 2020. Virulence determinants and biofilm formation in clinical isolates of Enterococcus: A cross-sectional study. J Acute Dis 9(1): 27-32.
Giridhara P. M., B. L. Umapathy and K. L. Ravikumar. 2011. Comparative study among clinical and commensal isolates of Enterococcus faecalis for presence of esp gene and biofilm production. Department of Microbiology, Kempegowda Institute of Medical Sciences, Banashankari II stage, Bangalore-560070, India, 28;5(5): 365-369.
Gustavo O. Z., M. P. Eliezer, P. S. Ricardo, M. T. Lu´cia, F. Jr. Jose´ and R. N. Ka´tia. 2011. Characterization of virulence factors and clonal diversity of Enterococcus faecalis isolates from treated dental root canals. Research in Microbiology (162) 151-158.
Haider, S. K. 2014. Detection of some Virulence Factors of Vancomycin-Resistant Enterococci. M.Sc. Thesis College of Science. Baghdad University. pp: 69-78.
Harley, J. P. and H. Prescott. 2002. Laboratory Exercises in Microbiology. 5th ed, The Mcgraw-Hill French pp: 98-105.
Hayes,J. R., L. L. English, P. J. Carter, T. Proescholdt, K. Y. Lee, D. D. Wagner, and D. G. White. 2003. Prevalence and antimicrobial resistance of Enterococcus Species isolated from retail meats. Appl. Environ. Microbiol., 69(12): 7153-7160.
Hossein S. K. and M. M. Ashraf. 2015. Assessment of biofilm formation by enterococci isolates from urinary tract infections with different virulence profiles. Journal of King Saud University – Science. 27 (4): 312-317.
Mi‑Ah K., R. Vinicius and M. Kyung‑San. 2020. Characterization of Enterococcus faecalis in different culture conditions. Nature Research 10(1):21867: 1-5.
Mohamed O. A. and E. B. Keith. 2018. Vancomycin-resistant enterococci: a review of antimicrobial resistance mechanisms and perspectives of human and animal health. Microbial Drug Resistance Vol. 24, N.5 Epidemiology. pp: 590-606.
Morello, J. A., H. E. Mizer, and P.A. Granato. 2006. Laboratory Manual and Work Book in Microbiology. 8th ed. McGraw-Hill Higher Education. New York. pp:95-99.
Papp-Wallace, K. M. A. Endimiani, M. A. Taracila, and R. A. Bonomo. 2011.
Carbapenems: Past, Present, and Future. Antimicrob. Agents Chemother. 55(11): 4943–4960.
Sedgley CM, SL Lennan and Clewell DB. 2004. Prevalence, phenotype and genotype of oral enterococci. Oral Microbiol Immunol 19, 95–101.
Sedláček I. 2007. Taxonomy of Prokaryotes. Masaryk University, Brno. 270(8) :24-27.
Shaimaa O. H. 2018. Effect of Biologically and Chemically Synthesized Silver Nanoparticles on Biofilm Forming Bacteria Isolated from Catheterized Iraqi Patients. Ph.D. Dissertation College of Medicine. Al-Qadisiyah University. pp: 88-95.
Susan A. 2015. Molecular Genetics Study of Enterococcus faecalis Isolated from Root Canal Infection of Human in Bagdad. M.Sc. Thesis College of Education for Pure Sciences / Ibn Al-Haitham of the University of Baghdad. pp:107-114.
Vesic D. and C. J. Kristich, 2012. MurAA is required for intrinsic cephalosporin resistance of Enterococcus faecalis. Antimicrob. Agents Chemother. 56(5):2443-2451.
Zoletti G. O, E M. Pereira, R P. Schuenck, LM. Teixeira and JF. Siqueira. 2011. Characterization of virulence factors and clonal diversity of Enterococcus faecalis isolates from treated dental root canals. Res Microbiol 162, 151-158.
Downloads
Published
Issue
Section
License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
How to Cite
2.jpg)
2.jpg)
