EFFICACY OF SILVER ZEOLITE ON HEMATOLOGY AND SURVIVAL RATE IN CYPRINUS CARPIO INFECTED WITH SAPROLEGNIA SPP.
DOI:
https://doi.org/10.36103/ijas.v55iSpecial.1901Keywords:
carp- clinoptilolite- copper sulphate-fungusAbstract
This study was undertaken to evaluate the effect of silver zeolite (SZ) on hematological parameters and survival rate in common carp, Cyprinus carpio infected with Saprolegnia spp. A total of 120 fish (weight 100 g) were divided into six treated groups in duplicate (10 fish/tank) as follows: T1, T2, T3 and T4 that infected with Saprolegnia spp. (2×104 zoospore/ml) and treated with SZ at (100,200 and 300 mg/l) while T4 infected with Saprolegnia spp. and treated with copper sulfate )5g/100 L(; C+ group, fish were infected with Saprolegnia without treatment and C- served as control negative group. Following 14 days from infection with Saprolegnia spp. and treatment with SZ, considerable changes were observed in blood indices. In pre-treatment, results showed a significant increased (P≤0.05) in WBC count in all infected fish (C+, T1, T2,T3 and T4) in comparison to C- group. RBC, Hb content and PCV% registered a significantly decreased (P≤0.05) in all infected fish (C+,T1, T2,T3 and T4) relative to the control group (C-). Post treatment, WBC registered significantly decreased in treated groups (T1,T2,T3 and T4) compared to C+ in WBC count. The best survival was recorded in T3 (85%) followed by T2 and T1 (65%, and 60%) respectively. In conclusion Silver zeolite seems to be a valuable disinfectant agent against Saprolegnia spp.; at the dose of 300 mg/l.
References
Abdulathem, A. A. and A. J. Al-Rudainy. 2021. Effect of dietary zeolite on ammonia toxicity and on some of blood parameters in common carp Cyprinus carpio. Iraqi Journal of Agricultural Sciences. 52(5): 1276–1283. https://doi.org/10.36103/ijas.v52i5.1465
Al-Hassani, T.S and S.A. Mustafa. 2022. Efficiency of synbiotic as feed additives on growth performance, survival rate and health status in common carp challenged with Saprolegnia spp. Iraqi Journal of Agricultural Sciences. 53(2): 397-405. https://doi.org/10.36103/ijas.v53i2.1548
Al-Rudainy, A.J., S.A. Mustafa, A.A. Ashor and M.T. Bader. 2023. The role of kaolin on hematological, biochemical and survival rate of Cyprinus carpio challenged with Pseudomonas aeruginosa. Iraqi Journal of Agricultural Sciences. 54(2): 472-477. https://doi.org/10.36103/ijas.v54i2.1723
Ashour, A. A., S. A. Mustafa and S. N. Yassein. 2017. Histopathological studies on common carp (Cyprinus carpio L.) infected with Saprolegnia spp. and treated with Virkon®. Mirror Res. Vet. Sci. Anim. 6(1): 19–30
Ashour, A. A., N. M. Salman, S. A. Mustafa and R. O. Nemah. 2019. Evaluation of hydrogen peroxide on controlling saprolegniasis in common carp, Cyprinus carpio L. Biochem. Cell. Arch. 19(2): 4247-4252
Fadhal, A. A. and S. A Mustafa. 2020. Influence of phytase enzyme on growth performance and survival rate challenged with Saprolegnia spp. in common carp. Iraqi Journal of Agricultural Sciences. 51(5): 1458–1465. https://doi.org/10.36103/ijas.v51i5.1156
Faik, H. H. and S.A. Mustafa. 2023. Role of vitamin C and E on genotoxicity, hematological and biochemical investigation in Cyprinus carpio following zinc oxide nanoparticals exposure. Iraqi Journal of Agricultural Sciences. 54(3): 716–723. https://doi.org/10.36103/ijas.v54i3.1752
Gill, T. S. and A. Epple. 1993. Stress-related changes in the hematological profile of the American eel (Anguilla rostrata). Ecotoxicol. Environ. Saf. 25(2): 227–235
Hamad, S.M. S A. Mustafa and A.M. Kane. 2019. Evaluation The Ozone Treatment to Control the Infection of Saprolegniasis in Cyprinus carpio L. J. Phys.: Conf. Ser. 1294.
Hlial, S,M. S. A. Mustafa and E.E. Obodi. 2021. Antifungal activity of curcumin- silver nanoparticles against Saprolegnia spp. in common carp. Plant Ach. 21(1): 148-154.
Jamalzadeh, H. R., A. Keyvan, M. R. Ghomi and F. Gherardi. 2009. Comparison of blood indices in healthy and fungal infected Caspian salmon (Salmo trutta caspius). Afr. J. Biotechnol. 8(2): 319-322
Johari, S. A., M. R. Kalbassi, M. Soltani, and I. J. Yu. 2016. Application of nanosilver-coated zeolite as water filter media for fungal disinfection of rainbow trout (Oncorhynchus mykiss) eggs. Aquac. Int. 24(1): 23–38
Juncey, K., and B. Ross. 1982. A Guide to Tilapia Feed and Feeding. Institute of aquaculture Universty of Striling. Scotland. 125 P
Madrid, A., P. Godoy, S. González, L. Zaror, A. Moller, E. Werner, M. Cuellar, J. Villena and I. Montenegro. 2015. Chemical characterization and anti-oomycete activity of Laureliopsis philippianna essential oils against Saprolegnia parasitica and S. australis. Molecules. 20(5): 8033–8047
McLeay, D. J. 1973. Effects of a 12-hr and 25-day exposure to kraft pulp mill effluent on the blood and tissues of juvenile coho salmon (Oncorhynchus kisutch). J. Fish. Res. B. Can.30(3): 395–400.
Mustafa, S.A. 2012. An Integrated Approach to Assess Impact of Environmental Stress in Carp, Cyprinus carpio L.: Biochemical, Genotoxic,Histopathological and Individual Level Effects, Ph.D. Dissertation,. Dept. of Biomed. and Biol. Sci.. Fac. of Sci.. Uniu. Plymouth. 77 P
Mustafa, S.A. and A.A. Ashor. 2021. Effect of chronic exposure to silver nanoparticles on histopathological manifestations of common carp, Cyprinus carpio L. Biochem. Cell. Arch. 21(1): 2719-2725
Mustafa, S.A., A.J. Al-Rudainy and K.A. AL-Faragi. 2019. Assessment of hydrogen peroxide on histology and survival rate in common carp, Cyprinus carpio L. infected with saprolegniasis. Iraqi Journal of Agricultural Sciences. 50(2):697-704. ttps://doi.org/10.36103/ijas.v2i50.669
Mustafa, S. A. and A. N. Jha. 2022. Impact of Hypoxia and Dead Zones on Habitat Destruction and Fish Population: In: K.K. Jha and M. Campbell (Eds) Dynamics and Interrelations Between Nature, Science, and Society, New York, p. 158-160.
Nikawa, H., T. Yamamoto, T. Hamada, M. B. Rahardjo, H. Murata and S. Nakanoda. 1997. Antifungal effect of zeolite incorporated tissue conditioner against Candida albicans growth and/or acid production. J. Oral Rehabil. 24(5): 350–357
Okumuş, İ. 2002. Rainbow trout broodstock management and seed production in Turkey: Present practices, constraints and the future. Turkish J. Fish. Aquat. Sci. 2(1): 41-56
SAS 2018. Statistical Analysis System, User’s Guide. Statistical. Version 9’, SAS. Institute Inc. USA
Seth, N. and K. K. Saxena. 2003. Hematological responses in a freshwater fish Channa punctatus due to fenvalerate. Bull. Environ. Contam. Toxicol B. 71(6): 1192–1199
Shah, A. F., A. S. Bhat, F. A. Bhat, M. H. Balkhi, A. Abubakr and I. Ahmad. 2015. Alteration in haemato-biochemical profiles of rainbow trout Oncorhynchus mykiss affected by Saprolegnia spp-A potential constraint for culture of trout in Kashmir Himalaya. Iran. J. Fish Sci.14(4): 970-984
Shah, S. L. and A. Altindag 2004. Hematological parameters of tench (Tinca tinca L.) after acute and chronic exposure to lethal and sublethal mercury treatments. Bull. Environ. Contam. Toxicol. B. 73(5): 911–918
Suhail, R. N., S.A. Mustafa, and E. E. AL-Obodi. 2022. Effeciency of silver nanoparticales as antibacterial against Aeromonas hydrophila isolated from infected common carp. Iraqi Journal of Agricultural Sciences. 53(3), 589-597. https://doi.org/10.36103/ijas.v53i3.1568
Trusch, F., L. Loebach, S. Wawra, , E. Durward, A. Wuensch, N. A. Iberahim, , I. De Bruijn, K. MacKenzie, A. Willems and A. Toloczko. 2018. Cell entry of a host-targeting protein of oomycetes requires gp96. Nat. Commun. 9(1): 1–12
Uchida, T., N. Maru, M. Furuhata, A. Fujino, S. Muramoto, A. Ishibashi, K. Koshiba, T. Shiba and T. Kikuchi. 1992. Anti-bacterial zeolite balloon catheter and its potential for urinary tract infection control. Hinyokika Kiyo. Acta Urologica Japonica. 38(8): 973–978
Yanong, R. P. 2003. Fungal Diseases in Fish. Black Sea J. Agric. 7–8
Zaki, M. S., O. M. Fawzi and J. E. Jackey 2008. Pathological and biochemical studies in Tilapia nilotica infected with Saprolegnia parasitica and treated with potassium permanganate. J. Agric. Environ Sci. 3(5): 677–680.
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