EFFECIENCY OF SILVER NANOPARTICALES AS ANTIBACTERIAL AGAINST AEROMONAS HYDROPHILA ISOLATED FROM INFECTED COMMON CARP
DOI:
https://doi.org/10.36103/ijas.v53i3.1568Keywords:
antimicrobial–avitek2-cyprinus carpio- minimum inhibitory concentration-nanosilverAbstract
The present investigation was carried out to investigate the antibacterial efficiency of silver nanoparticles (AgNPs) in vitro against Aeromonas hydrophila using disc diffusion assay and minimum inhibitory concentration. The pathogenic A. hydrophila was isolated from infected common carp, usually diagnosed by chemical methods, and Avitek 2 compact device were used to confirm the diagnosis. The effectiveness of the prepared AgNPs was tested by chemical and biological (green synthesis using lemon extract) methods and were diagnosed by Fourier-transform infrared spectroscopy (FTIR), UV-Visible spectroscopy, Transmission electron microscopy (TEM), scanning electron microscope (SEM), which was spherical shape of the nanosilver and the size ranged between 30-50 nm. Results of disc diffusion assay showed that the chemical synthesized of AgNPs in 18hr recorded the highest inhibition zone followed by the bio-synthesized AgNPs and Oxytetracycline respectively. After 24 hr the highest inhibition zone was registered in Oxytetracycline, however after 5 days bio-synthesized AgNPs showed the higher inhibition zone which was significantly different(P≤0.05) in comparison to other products. Based on these results, both bio and chemical synthesized of AgNPs were effectively act as antibacterial against A.hydrophila. However, green synthesis using lemon extract is considered better antibacterial with low MIC than chemical AgNPs because lemon extract is regarded eco-friendly and also the low cost product compared to chemical AgNPs synthesis.
References
Abdelhamed, H., I. Ibrahim, W. Baumgartner, M.L. Lawrence and A. Karsi. 2017. Characterization of histopathological and ultrastructural changes in channel catfish experimentally infected with virulent Aeromonas hydrophila. Front. Microbiol. 8: 1-15
Al- Abodi, E. E,. Latif. I. and A. Farouk. 2018. Iraqi Patent No. Y10S977/73 C08J5/10 Baghdad, Iraq: central Organization for Standardization and Quality Control
Al-Fatlawy, H. N.K.and H. A. Al-Hadrawy. 2014. Isolation and characterization of A. hydrophila from the Al-Jadryia river in Baghdad (Iraq). American Journal of Educational Res. 2(8):658-662
Algammal, A. M., M. F. Mohamed, B. A. Tawfiek, W. N .Hozzein, W. M. El Kazzaz, and M. Mabrok. 2020. Molecular typing, antibiogram and PCR-RFLP based detection of Aeromonas hydrophila complex isolated from Oreochromis niloticus. Pathogens. 9(3): 238
Al-Rudainy, A. J. and H.A. Khalel. 2019. Histopathological changes (gills and liver) and clinical sings of common carp, Cyprinus carpio L. exposed to graphene nanoparticles. Iraqi J. Agric. Sci. 50(3):901-908
Al-Saphar, S. A.2012. Production of Alocal Probiotic and Its Effect on Growth of Common Carp Cyprinus carpio L. and Resistance to Pathogenic Bacteria Aeromonas hydrophila . M.Sc. Thesis, Baghdad. Vet. Medicen, Baghdad University. 4:51-55.
Al-Shmgani, H.S., W.H. Mohammed, G.M. Sulaiman and A.H. Saadoon. 2017. Biosynthesis of silver nanoparticles from Catharanthus roseus leaf extract and assessing theirantioxidant, antimicrobial, and wound-healing activities. Artif. Cells Nanomed. Biotechnol. 45: 1234–1240.
Asgary, V., A. Shoari, F. Baghbani-Arani, S. A. Shandiz, M. S. Khosravy, A. Janani and R. A. Cohan. 2016. Green synthesis and evaluation of silver nanoparticles as adjuvant in rabies veterinary vaccine. Int. J. nanomedicine, 11: 3597-360
Bauer, H., F. ParonettoBurns, W. A. and A.Einheber. 1966. The enhancing effect of the microbial flora on macrophage function and the immune response: a study in germfree mice. The Journal of Experimental Medicine, 123(6), 1013-1024
Chaloupka, K., Y. Malam and A. M. Seifalian. 2010. Nanosilver as a new generation of nanoproductin biomedical applications. Trends Biotechnol,28(11):580-588.
Dadosh, T. 2009. Synthesis of uniform silver nanoparticles with a controllable size. Materials Letters, 63(26): 2236-2238
El-Son, M. A., N. K. Abdelkhalek, A. M. El-Ashram and V. H. Zaki. 2019. Phenotypic and biochemical detection of Aeromonas hydrophila isolated from cultured Oreochromis niloticus during disease outbreaks. Int. J. Fish. Aquat. Stud. 7: 197-202
Halawai, B. K. 2003. Extra Chromosomal DNA Diversity and Drug Resistance in Aeromonas hydrophila of Aquaculture systems (Doctoral dissertation, Central Marine Fisheries Research InstitutE.24-102
Ibrahim, O. M. S., A. H. Saliem and S. I. Salih. 2016. Antibacterial activity of silver nanoparticles synthesized by Cinnamon zeylanicum bark extract against Staphylococcus aureus. Al-Anbar J. Vet. Sci. 9(1): 22-36
Igbinosa, I. H., E.U. Igumbor, F. Aghdasi, M. Tom and A.I. Okoh. 2012. Emerging Aeromonas species infections and their significance in public health. Sci. World J. 2012: 1-13.
Julinta, R. B., A. Roy, J. Singha, T. J. Abraham and P. K. Patil. 2017. Evaluation of efficacy of oxytetracycline oral and bath therapies in Nile tilapia, Oreochromis niloticus against Aeromonas hydrophila infection. Int. J. Curr. Microbiol. Appl. Sci. 6(7) : 62-76.
Korbekandi, H. and S. Iravani. 2012. Silver nanoparticles. In The delivery of nanoparticles. IntechOpen, 57: 1-11
Laith, A.R. and M. Najiah. 2014. Aeromonas hydrophila: antimicrobial susceptibility and histopathology of isolates from diseased catfish, Clarias gariepinus (Burchell). J. Aquac. Res. Development. 5(2): 2-7
Mog, M., S. Ngasotter, S. Tesia, D. Waikhom, S. P. Panda, S. Sharma and S. Varshney. 2020. Problems of antibiotic resistance associated with oxytetracycline use in aquaculture: A rev. J. Entomol. Zoolo. Studies, 8: 1075-1082
Morones, J. R., J. L. Elechiguerra, A. Camacho, K. Holt, J. B. Kouri, J. T. Ramírez and M. J. Yacaman. 2005. The bactericidal effect of silver nanoparticles. N5anotechnology, 16(10): 2346–2353
Muduli, C., G.Tripathi, K. Paniprasad, K. Kumar, R. K. Singh and G. Rathore. 2020. Virulence potential of Aeromonas hydrophila isolated from apparently healthy freshwater food fish. Biologia, 76:1005–1015.
Owuama, C. I. 2017. Determination of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) using a novel dilution tube method. African J. Microbiol. Res. 11(23): 977-980
Pincus, D. H. 2006. Microbial Identification Using The BioMérieux Vitek® 2 system. Encyclopedia of Rapid Microbiological Methods. Bethesda, MD: Parenteral Drug Association, 1-32
Qais, F. A., A. Shafiq, H. M. Khan, F. M. Husain, R. A. Khan, B. Alenazi and I. Ahmad. 2019. Antibacterial effect of silver nanoparticles synthesized using Murraya koenigii (L.) against multidrug-resistant pathogens. Bioinorganic Chemistry and Applications, 2(4):30-32
Rashid, M. M., M. S. Hossain,. S. M. and M. F. Ali. 2013. Isolation and identification of Aeromonas hydrophila from silver carp and its culture environment from Mymensingh Reg. J. of the Bangladesh Agri C. University, 11(2):373-376
Saavedra, M.J., S. Guedes-Novais, A. Alves, P. Rema, M. Tacão, A. Correia and A. Martínez-Murcia. 2004. Resistance to beta-lactam antibiotics in Aeromonas hydrophila isolated from rainbow trout (Oncorhynchus mykiss). Int. Microbiol. 7(3): 207-211
SAS. 2012. Statistical Analysis System, User's Guide. Statistical. Version 9.1th ed. SAS. Inst. Inc. Cary. N.C. USA Science,5:191-199
Siddiqi, K. S. and A. Husen. 2016. Green synthesis, characterization and uses of palladium/platinum nanoparticles. Nanoscale Res. Letters, 11(1): 1-13
Swain, P., S. K .Nayak, A .Sasmal, T. Behera, S. K. Barik, S. K. Swain and P. Jayasankar. 2014. Antimicrobial activity of metal based nanoparticles against microbes associated with diseases in aquaculture. W. J. of Microbiology and Biotechnology, 30(9): 2491-2502
Torres, J. L. 1990. Studies on Motile Aeromonas spp. Associated With Healthy and Epizootic Ulcerative Syndrome-Positive Fish (Doctoral dissertation, University Pertanian Malaysia), 3:26-64
Vimbela, G. V., S. M. Ngo, C. Fraze, L. Yang and D. A. Stout. 2017. Antibacterial properties and toxicity from metallic nanomaterials. Int. J. nanomedicine, 12: 3941-3965
Wong, K. K. and X. Liu. 2010. Silver nanoparticles—the real “silver bullet” in clinical medicine?, Med. Chem. Comm. 1(2):125-131
Yaqoob, A. A., T. Parveen, K. Umar and M. N. Mohamad Ibrahim. 2020. Role of nanomaterials in the treatment of wastewater: A review. Water, 12(2): 495.
Zorriehzahra, M. J., L. Yazdanpanah-Goharrizi, F. Rokhbakhsh-Zamin, N. Kazemipour, and B. Kheirkhah. 2020. Isolation, biochemical and molecular detection of Aeromonas hydrophila from cultured Oncorhynchus mykiss. Iranian Journal of Fisheries Sciences, 19(5):2422-2436.
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