EFFECT OF CHEMICAL MEDIA COMPONENTS AND SILVER NANOPARTICLES ON THE INDUCTION OF CALLUS CULTURE AND ACCUMULATION OF SECONDARY COMPOUNDS IN A Trigonella foenum-graecum L.
DOI:
https://doi.org/10.36103/ijas.v55iSpecial.1891Keywords:
in vitro, nanoparticles, flavonoids, alkaloids.Abstract
This study was investigate the effect of components media and silver nanoparticles (Ag-NPs) synthesized in the laboratory on the induction of biomass and secondary metabolites of calli cultures induced from the cotyledons of Trigonella foenum-graecum L. The culture media LS and B5 were tested with the growth regulators 2,4-Dichlorophenoxyacetic acid (2,4-D) at a concentration of 1.0, 2.0 and 3.0 mg l-1 and 6-benzyladenine (BA) at a concentration of 0.0, 0.5, 1.0, 1.5 and 2.0 mg l-1, with the aim of studying its effect on callus induction. In comparison, Ag-NPs were used at a concentration of 0.0, 0.5, 1.0, 1.5 and 2.0 mg l-1 to study its effect on the production of some secondary compounds. The results showed that the mixture consisting of the culture media LS with 3.0 mg l-1 of 2,4-D and 1.0 mg L-1 of BA the highest percentage of callus induction and the minimum days to complete callus formation. While, the same media with 2.0 mg l-1 2,4-D and 1.0 mg l-1 of BA recorded the highest dry weight (DW) and relative water content (RWC). The combination B5×2.0 mg l-1 2,4-D×1.5 or 2.0 mg l-1 showed the highest fresh weight (FW) and the lowest time required to start callus induction, respectively. As for the production of SC, the B5 media with 2.0 mg l-1 of Ag-NPs achieved the highest concentration of alkaloid compounds represented by trigonelline and diosgenin, and flavonoids expressed by qurcetine, vitxein, rutin, apigenin, isovitexin, and kaemferol.
References
Aasim, M., F.S. Baloch, M.A. Nadeem, A. Bakhsh, M. Sameeullah, and S. Day, 2018. Fenugreek (Trigonella Foenum-graecum L.): An underutilized edible plant of modern world. Global Perspectives on Underutilized Crops, 381–408.
Ali, A., S. Mohammad, M.A. Khan, N.I. Raja, M. Arif, A. Kamil, and Z.R. Mashwani, 2019. Silver nanoparticles elicited in vitro callus cultures for accumulation of biomass and secondary metabolites in Caralluma tuberculata. Artifi, Cell, Nanomed. Biotechnol. 47(1): 715–724
Bhatla, S.C. and Lal, M.A. 2018. Plant Physiology, Development and Metabolism. Springer, pp: 1237
Djeridane, A., M. Yousfi, B. Nadjemi, D. Boutassouna, P. Stocker, and N. Vidal, 2006. Antioxidant activity of some algerian medicinal plants extracts containing phenolic compounds. Food Chem. 97: 654–660
Fazal, H., B.H Abbasi, N. Ahmad, and M. Ali, 2016. Elicitation of medicinally important antioxidant secondary metabolites with silver and gold nanoparticles in callus cultures of Prunella vulgaris L. Appl Biochem Biotechnol., 180:1076–1092
Gamborg, O.L., R.A. Miller, and K. Ojima, 1968. Nutrient requirements of suspension cultures of soybean root cells. Expert. Cell Res, 50: 151-158
Gupta, S.D., A. Agarwal, and S. Pradhan, 2018. Phytostimulatory e_ect of silver nanoparticles (AgNPs) on rice seeding growth: An insight from antioxidative enzyme activities and gene expression patterns. Ecotoxicol. Environ. Saf, 161: 624–633
Ibrahim, K.M. 2017. Applications in Plant Biotechnology, College of Biotechnology, Al-Nahrain Univ., pp: 680
Karimi, S, A. Yadollahi, R. Nazari-Moghadam, A. Imani, and K. Arzani, 2012. In vitro screening of almond (Prunus dulcis (mill.)) genotypes for drought tolerance. J Biol Environ Sci, 6:263–270
Kenny, O., T. J. Smyth, C.M. Hewage, and N.P. Brunton, 2013. Antioxidant properties and quantitative UPLC–MS analysis of phenolic compounds from extracts of fenugreek (Trigonella foenum-graecum) seeds and bitter melon (Momordica charantia) fruit. Food Chem¸ 141: 4295–4302
Lee, W.M, J.I. Kwak, and Y.J. An, 2012. Effect of silver nanoparticles in crop plants Phaseolus radiatus and Sorghum bicolor: media effect on phytotoxicity. Chemosphere, 86: 491–499
Li, P., Y. Mou, S. Lu, W. Sun, J. Lou, C. Yin, and L. Zhou, 2012. Quantitative determination of diosgenin in Dioscorea zingiberensis cell cultures by microplate-spectrophotometry and high-performance liquid chromatography. Afri J Pharm. Pharmacol., 6(15): 1186 – 1193
Linsmair, E.M. and F. Skoog, 1965. Organic growth factor requirement of tobacco tissue culture. Physiol. Plant, 18: 100–27
Luhata, L.P., C.N. Chick, N. Mori, K. Tanaka, H. Uchida, T. Hayashita and T. Usuki, 2022. Synthesis and antioxidant activity of silver nanoparticles using the odontonema strictum leaf extract. Molecules, 27(3210): 1-9
Mehrdel, B. and A.A. Aziz, 2018. The sensitivity of surface plasmon resonance damping for colloidal silver nanoparticles. J. Physic., Conference Series, 1083(1): 1-9
Murashige, T. and F. Skoog, 1962. A revised medium for rapid growth and bioassays with tobacco cultures. Physiol. Plant., 15: 473-497
Neamah, S.I. and A.F. Almehemdi, 2017. Extraction of natural compounds from callus inducted of common sage plant Salvia officinalis L. and their evalution of antioxidant activity. Iraq J Agri Sci, 48 (6): 1541-1548
Neamah, S. I. 2020. Inducing some secondary metabolites from callus culture derived from Plantago psyllium and Plantago major exposed to cobalt stress. Iraq J Agri Sci, 51(3): 938-943.
Salih, A.M., F. Al-Qurainy, S. Khan, M. Nadeem, M. Tarroum, and H.O. Shaikhaldein, 2022. Biogenic silver nanoparticles improve bioactive compounds in medicinal plant Juniperus procera in vitro. Front Plant Sci., 26(13): 962112
Seif Sahandi, M., A. Sorooshzadeh, H.S. Rezazadeh, and H.A. Naghdibadi, 2011. Effect of nanosilver and silver nitrate on seed yield of borage. J Med Plants Res, 5: 706–710
Shakeran, Z., M. Keyhanfari, G. Asghari, and M. Ghanadian, 2015. Improvement of atropine production by different biotic and abiotic elicitors in hairy root cultures of Datura metel. Turk J Biol, 39:111–118
Sindhu, G., G.L. Shyni, S., C.K. Pushpan, B. Nambisan, and A. Helen, 2018. Evaluation of anti-arthritic potential of Trigonella foenum-gracum L. (Fenugreek) mucilage against rheumatoid arthritis. Prostag Oth Lipid M, 138: 48 – 53.
Syed, O.A., Z. Rashid, M.H. Ahmad, R. Shukat, A. Ishaq, N. Muhammad and H.U. Rahman, 2020. Nutritional and therapeutic properties of fenugreek (Trigonella foenum-graecum): a review. Int J Food Proper, 23 (1): 1777–1791
Omezzine, F., M. Bouaziz, M. Daami-Remadi, M.S.J. Simmonds, and R. Haouala, 2014. Chemical composition and antifungal activity of Trigonella foenum-graecum L. varied with plant ploidy level and developmental stage. Arab J Chem, 10(2): 3622-3631
Park, H.J., K.S. Lee, E.K. Lee, and N.C. Park, 2019. Efficacy and safety of a mixed extract of Trigonella Foenum-graecum seed and Lespedeza cuneate in the treatment of testosterone deficiency syndrome: A randomized, double-blind, placebo-controlled trial. J. Urol., 201
Pidlisnyuk, V., T. Stefanovska, O. Zhukov, A. Medkow, P. Shapoval, V. Stadnik, and M. Sozanskyi, 2022. Impact of plant growth regulators to development of the second generation energy crop Miscanthus×giganteus produced two years in marginal post-military soil. Appl. Sci., (12881): 1-14
Prosposito, P., L. Burratti, and I. Venditti, 2020. Silver nanoparticles as colorimetric sensors for water pollutants. Chemosensors, 8(2): 1-26
Radovanovic, B., J. Mladenovic, A. Radovanovic, R. Pavlovic, and V. Nikolic, 2015. Phenolic composition, antioxidant, antimicrobial and cytotoxic activities of Allium porrum L. (serbia) extracts. J. Food Nut. Res., 3 (9): 564-569
Ruwali, P., N. Pandey, K. Jindal, and R. V.Singh. 2022. Fenugreek (Trigonella foenum-graecum): nutraceutical values, phytochemical, ethnomedicinal and pharmacological overview. S Afr J Bot, 151, 423-431
Ruaa, M. D.; S.A.Alsahib and.F. Ascar. 2021. Synthesis, characterization and cyclazation of pyran using Ag2O nanoparticle from natural source “ginger”. Iraqi Journal of Agricultural Sciences, 52(5):1171-1184. https://doi.org/10.36103/ijas.v52i5.1455
Suhail, R.N. S.A.Mustafa, and E.E. AL-Obodi. 2022. Efficiency of silver nanoparticales as antibacterial against Aeromonas hydrophila isolated from infected common carp. Iraqi Journal of Agricultural Sciences, 53(3):589-597. https://doi.org/10.36103/ijas.v53i3.1568
Vinay, S. and A. Afroz, 2015. Plant Tissue Culture. Springer, pp: 529
Wani, S.A. and P. Kumar, 2018. Fenugreek: A review on its nutraceutical properties and utilization in various food products. J. Saudi Society Agri. Sci ., 17: 97 – 106
Yaaqoob, L.A., R.M. Abed, Z.K. Kamona, M.F. Altaee, and R.W. Younis. 2022. Evaluation the ability of titanium oxide nanoparticles to increase the production of prodigiosin and phenasine from Serratia marcescens and Pseudomonas aeruginosa, respectively. Iraqi Journal of Agricultural Sciences, 53(3):496-504. https://doi.org/10.36103/ijas.v53i3.1557
Yasothai R. 2021. Chemical composition of fenugreek (Trigonella foenum-graecum L.) seed and galactomannan depleted fenugreek residue. The Pharma Innovation J., 10(7): 944-947.
Zhang, S., Y. Liu, S. Shen, Z. Liang, and D. Yang, 2011. Effect of elicitors on accumulation of phenolic acids and tanshinones in Salvia miltiorrhiza hairy root. Zhongguo Zhong Yao Za Zhi., 36: 1269-1274
Downloads
Published
Issue
Section
License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
2.jpg)
https://orcid.org/0000-0002-5774-5906 2.jpg)
