MINITUBERS PRODUCTION OF FOUR POTATO (SOLANUM TUBEROSUM L.) CULTIVARS BY TISSUE CULTURE TECHNIQUE

Authors

DOI:

https://doi.org/10.36103/ijas.v53i5.1619

Keywords:

minitubers, potato cultivars, Solanum tuberosum L., plant tissue culture

Abstract

This study was aimed to produce potato minitubers via tissue culture technique by testing four cultivars; Agria, Nicola, SM 12-124-15 and JIP 1600-1 under in vitro, greenhouse and open-air field conditions. Nicola cultivar was superior upon the other cultivars under in vitro and greenhouse conditions by producing the best shoot multiplication (1.89 shoots/ explant and 13..27 leaves/ explant) and the highest number of minitubers (8.87 minitubers/ plant) in greenhouse whereas, SM 12-124-15 was better in the open field conditions by producing 11.25 tubers per plant, recording the highest mean weight of tubers (321.33 g), the highest weight of tubers per plant (3.615 kg), the bigger sizes of tubers (length 8.22 cm: width 3.43 cm) and the highest total yield per hectare (49.70 Ton/ ha). An interesting microtubers formation was noticed on SM 12-124-15 explants grown on MS medium supplemented with 0.5 mg/l-1 BA. The main conclusion drown from the current study is MS medium with basic vitamins and solidified by agar without the need of adding exogenous plant growth regulators can be used for mass seed tubers production based on potato genotype in vitro under optimized culture conditions with a low cost propagation system and shorter period of time than normal minitubers production process.

References

Asma, R., B. Askari, N. A. Abbasi, M. Bhatti and A. Quraishi .2001.. Effect of growth regulators on in vitro multiplication of potato. Int. J. Agri. Biol., 3: 181- 182

Badoni, A. and J. S. Chauhan 2010.. In vitro Sterilization Protocol for Micropropagation of Solanum tuberosum cv. Kufri Himalini. Academia Arena, 2(4).

Bostan, H. and E. Demirel .2004.. Obtaining PVX, PVY and PLRV-free micro tuber from Granola, Pasinler 92 and Casper potato (Solanum tuberosum L.) cultivars. Pak. J. Biol. Sci., 7(7): 1135-1139

Chandra R. and R. K. Birhman 1994. In vitro micropropagation in relation to pedigree in potato. J. Indian Potato Assoc. 21(1&2):87

Ebad, F. A. S., M. E. L. El-Sadek and A. A. El-Kazzaz 2015. Micropropagation of four potato cultivars in vitro. Academia Journal of Agricultural Research, 3(9), 184-188

Everson, J. and G. Renan 2003. Toxicidade de antibióticos no cultivo in vitro da batata em meios semi-sólido e líquido. Pesq. agropec. bras, Brasília, v. 38, n. 11, p. 1273-1279

Hoque, M.E. 2010. In vitro tuberization in potato (Solanum tuberosum L.). Plant Omics J., 3: 7-11

Fadladeen, L. H. and R. S. Toma, .2020. Embryo culture and in vitro clonal propagation of oak (Quercus aegilops L.). The Iraqi Journal of Agricultural Science, 51(1), 347-355

Jones E.D. 1991 Progress in seed potato technology. American Journal of Potato Research, 68(4), 247 – 248

Khadiga G. A., S. M. Rasheid and M. K. Mutasim .2015. Micro tuber induction of two potato (Solanum tuberosum L.) varieties namely, Almera and Diamant. Int. Res. J. Biol. Sci. 4(3): 84-89

Otroshy M. 2006. Utilization of tissue culture techniques in a seed potato tuber production scheme. PhD Thesis, Wageningen University, Wageningen, The Netherlands, 264 p

Qureshi M. A., Z. Gul, S. Ali, and A. R. Khan 2014. Comparative growth response of potato plantlets developed on liquid vs solidified (MS) medium using tissue culture technology. Int. J. Recent Sci. Res. 5(4):736-739

Murashige T. and F. Skoog 1962. A revised medium for rapid growth bioassay with tobacco tissue cultures. Physiol. Plant. 15(3): 473-497

SAS. 2001. SAS/ STAT User’s Guide for Personal Computers. Release 6.12. SAS Institute Inc. Cary, NC, USA

Steward, F. C., and S. M. Caplin 1951. A tissue culture from potato tuber: the synergistic action of 2, 4-D and of coconut milk. Science, 113(2940), 518-520

Struik P.C. and S. G. Wiersema 1999 Seed potato technology. Wageningen academic publishers Wageningen, The Netherlands, 383 p

Toma, R. S. 2018. Micropropagation response of three grape vines (Vitis vinifera L.) cultivars to ferrous, nitrate and growth regulators. Vegetos-An International Journal of Plant Research, 31(3), 126-131

Van Staden, J. and G. G. Dimalla 1976. Endogenous cytokinins and tuberization in the potato (Solanum tuberosum L.). Annals of Botany, 40(169), 1117-1119

Vinterhalter, D., I. Dragicevic and B. Vinterhalter, . 2008. Potato in vitro culture techniques and biotechnology. Potato I. Fruit, vegetable and cereal science and biotechnology, 2, 16-45

Wang, Y., M. A. R. Rashid, X. Li, C. Yao, L. Lu J. Bai and Z. Pan 2019. Collection and evaluation of genetic diversity and population structure of potato landraces and varieties in China. Frontiers in plant science, 10, 139

Wang P.J. , and C. V. Hu .1982.. In vitro mass tuberization and virus free seed potato production in Taiwan. Am. Pot. J. 59(1): 33-39

Yaseen, F. K., R. S. Toma, and D. Carbonera .2017. The Effects of Vitamins on Micropropagation of Desiree and Mozart Potatoes (Solanum Tuberosum L.). Science Journal of University of Zakho, 5(1), 53-56.

Downloads

Published

2022-10-25

How to Cite

Toma, R. S. (2022) “MINITUBERS PRODUCTION OF FOUR POTATO (SOLANUM TUBEROSUM L.) CULTIVARS BY TISSUE CULTURE TECHNIQUE”, IRAQI JOURNAL OF AGRICULTURAL SCIENCES, 53(5), pp. 1058–1066. doi: 10.36103/ijas.v53i5.1619.

Issue

Section

Articles