IN VITRO ANTIMICROBIAL ACTIVITY OF Lactobacillus parabuchneri NU14 AS A PROBIOTIC
DOI:
https://doi.org/10.36103/ijas.v54i6.1864Keywords:
toothpaste formula, oral diseases, bacteriocin.Abstract
The present study was designed to select a suitable isolate of Lactobacillus sp. in order to use it as a probiotic in a toothpaste for the prevention of dental disease. For this purpose, 30 Lactobacillus isolates were collected from different sources and subjected to a screening program to evaluate their antagonism activities against three oral pathogens: Staphylococcus aureus, E. coli and Streptococcus mutans. Eight Lactobacillus isolates were selected from primary screening according to their ability to inhibit the growth of three indicators which then exposed to a secondary screening to detect their ability to produce high amount of bacteriocin. Based on results, the isolate Lactobacillus parabuchneri.Nu14 was selected as a highest bacteriocin producing isolate. Next, several tests were performed to study the properties of Lactobacillus parabuchneri Nu14, in particular its biosafety and its suitability to be a successful probiotic. Results showed that Lactobacillus parabuchneri was resistance to β-lactams group such as penicillin and ampicillin whereas, it was sensitive to amoxicillin-clavulanate. In addition, results revealed that this bacterium was strongly adherent and good biofilm producer, has the ability to survive in pH 2 for 2hr; has the ability of surviving in the presence of 85.2% of lysozyme after 90 min; tolerant to 0.3% of bile salts for 2hr; had high auto-aggregation capability and high ability of adhering with S. mutans. Two toothpaste formulas were selected and optimized to choose the most useful one that can be used in this study. Based on results, L. parabuchneri Nu14 was successfully survive in formula II over 21 days.
References
Abd, F.N. and K. Luti, 2017. An Exploitation of Interspecies Interaction for Promoting Bacteriocin Production by Local Isolate of Bacillus sp. M.Sc. Thesis. Department of Biotechnology, College of science, University of Baghdad, pp: 85-86
Aihua Z., W. Shouli, C. Wenlian, Z. Xiaojiao, H. Fengjie, H. Xiaolong, G. Kun, R. Cynthia, H. Yanxia, Y. Herbert, and Z. J., Jinshui, 2020. Increased levels of conjugated bile acids are associated with human bile reflux Qastritis; 14:10(1):11601
AL-Gbouri and A. G. Hamzah, 2018. Evalution pf phyllanthus emblica extracts as antibacterial and antibiofilm against biofilm formation bacteria. Iraqi Journal of Agricultural Sciences; 49(1):22-40. https://doi.org/10.36103/ijas.v49i1.217
Ali Saadi Al-Baer; and A.H. Asmaa, 2017. Isolation and identification of Escherichia coli producing cytosine deaminase from Iraqi patients. International Journal of Advanced Research in Biological Sciences; 4:2348-9069
Annica Almstahl, L. Peter, E. Lars, and C. Anette, 2013. Fermentation of sugars and sugar alcohols by plaque Lactobacillus strains. Clinical Oral Investigations; 1465–1470
Bautista-Trujillo G. U., J. L. Solorio-Rivera, I. Renter ́ıa-Solo ́rzano, S. I. Carranza-German, J. A. Bustos-Martınez, R. I. Arteaga-Garibay, V. M. Baizabal-Aguirre, M. Cajero-Jua ́rez, A. Bravo-Patino and J. J. Valdez-Alarcon, 2013. Performance of culture media for the isolation and identification of Staphylococcus aureus from bovine mastitis; Journal of Medical Microbiology. 62, 369–376
Bei Zhang, W. Yanping and H. Qunce, 2016. Screening of probiotic activities of lactobacilli strains isolated from Traditional tibetan qula, a raw yak milk cheese. Asian Australas. Journal Anim. Sci; 29(10): 1490-1499
Christensen GD, WA. Simpson, JA, 1995. Adherence of coagulase negative Staphylococci to plastic tissue cultures: a quantitative model for the adherence of Staphylococci to medical devices. J Clin Microbiol; 22:996-1006
Collado, M. C., E. Isolauri, K. Laitinen and K., S. Salminen, 2010. Effect of mother’s weight on infant’s microbiota acquisition, composition, and activity during early infancy: A prospective follow-up study initiated in early pregnancy. The American Journal of Clinical Nutrition; 92(5), 1023–1030
De Keersmaecker SC, TL Verhoeven, J Desair, J Vanderleyden, and I Nagy, 2006. Strong antimicrobial activity of Lactobacillus rhamnosus GG against Salmonella typhimurium is due to accumulation of lactic acid. FEMS Microbiology Letter; 259: 89-96.
Devay, J.E., and W.C. Schnathorst, 1963. Single-cell isolation and preservation of bacterial cultures. Nature; 199, 775–777
Ehrmann, M., P. Kurzak, J. Bauer, and R. Vogel, 2002. Characterization of lactobacilli towards their use as probiotic adjuncts in poultry. J. Appl. Microbiol; 92, 966–975
Ferreira, C.L, L. Grześkowiak, M.C. Collado, and S. Salminen, 2011. In vitro evaluation of Lactobacillus gasseri strains of infant origin on adhesion and aggregation of specific pathogens. J. Food Prot; 74, 1482–1487
Freeman J, FR Falkiner, and CT Keane, 1989. New method for detecting slime production by coagulase negative Staphylococci. J Clin Pathol; 42:872-4
Gaurav K.S, G. Jayesh and D Meenakshi, 2018. Textbook of Cosmetic Formulations, Pages: 74-78
Gianfranco Pannella, J. Silvia, C. Francesca, V. Franca, I. Massimo, S. Mariantonietta, T. Patrizio, I. Caterina, S. Elena, and C. Raffaele, 2020. Effect of biofilm formation by Lactobacillus plantarum on the malolactic fermentation in model Wine. National Library of Medicine; 17;9(6):797
Haukioja A, V. Loimaranta, and J. Tenovuo, 2018. Probiotic bacteria affect the composition of salivary pellicle and streptococcal adhesion in vitro. Oral Microbial Immunology; 23(4):336–343
Holt, J.C. and N.R. Krieg, 1984 .Bergeys’ Manual of Systemic Bacteriology .4th (ed).William and Willkins,Baltimor.London; 9: 40-97
Israa T. and K. Luti, 2021. Probiotic application of vaginal Lactobacillus crispatus against some genitourinary tract pathogens. M.Sc. thesis. Department of biotechnology, Collage of science, University of Baghdad, pp:75-78.
Jorn A Aas, 2005. J Clin Microbiol. Defining the normal bacterial flora of the oral cavity. National Library of Medicine; 43 (11): 5721-32.
Jose E J, S. Padmanabhan, and B. A. Chitaranjan, 2013. Systemic consumption of probiotic curd and use of probiotic toothpaste to reduce Streptococcus mutans in plaque around orthodontic brackets. American Journal of Orthodontics and Dentofacial Orthopedics; 144: 67-72
Jukka H M. A role for oral health personnel in stroke prevention, 2017. National Library of Medicine; (8):26-28
Karaqlu, A. ; F. Aydin and S. Kilic. 2003. Antimicrobial activity and characteristics of bacteriocins produced by vaginal Lactobacilli. Turk.J.Med.Sci; 33:7-13
Karita M N, H. Jukka M, M. Anna, O. Jussi, O. Fernanda, M. Hellevi: 2018. Oral health in patients with renal disease: a longitudinal study from predialysis to kidney Transplantation; 22(1):339-347
Khaleel M. M. and A. A. Thaer. 2017. Using probiotic and inulin to prolong fermented dairy products shelef life. Iraqi Journal of Agricultural Sciences; 48 (2): 608-617. https://doi.org/10.36103/ijas.v48i2.428
Kos, B. V., J. Šušković, , S. Vuković, , M. Šimpraga, J. Frece, and S. Matošić, 2003. Adhesion and aggregation ability of probiotic strain Lactobacillus acidophilus M92. Journalcof applied microbiology; 94(6), 981-987.
Kamona, Z. K. A. and A.H. H. Alzobaay, 2021. Effect of essential oil extract from lemongrass (Cymbopogon citratus) leaves on viability of some pathogenic bacteria and sensory properties of fish balls. Iraqi Journal of Agricultural Sciences, 52(2): 268-275. https://doi.org/10.36103/ijas.v52i2.1288
Loesche WJ, 1996. "Ch. 99: Microbiology of dental decay and periodontal disease". In Baron S; et al. (eds.). Baron's Medical Microbiology (4th ed.). University of Texas Medical Branch. ISBN 978-0-9631172-1-2. PMID 21413316
Mahmoud S. T. and K. Luti. 2015. Enhancement of prodigiosin production by Serratia marcescens S23 via introducing microbial elicitor cells into culture medium. Iraqi. Journal of Science; 56(3A): 1938-1951
Mallikarjuna K, S. Gupta, S. Singh, B. Dadarya, P. Dausage, and P. Gupta, 2013. Probiotics in dentistry: review of the current status. International Journal of contemporary Dentistry; 4: 66-75
Manchenko GP, 1994. "Lysozyme": handbook of detection of enzymes on electrophoretic gels. Boca Raton, Fla.: CRC Press. pp. 223. ISBN 978-0-8493-8935-1
Maria Diaz, L. Victor, D. Beatriz, R. Begoña, F. and M. María, A, Cruz, 2016. Miguel. biofilm-forming capacity in biogenic amine-producing bacteria isolated from dairy products. National Library of Medicine; 12;7:591
Nagraj T, B Ravi, SN Sankara, and K Madhu, 2012. Probiotics and oral health. Journal of Indian Academy of Oral Medicine and Radiology; 24: 146-8
Nouralhuda D. and K. Luti, 2019. Study the optimal conditions for the production of lactic acid bacteria producing bacteriocin and evaluation of its probiotic potential. Thesis. Department of Biotechnology, College of Science, University of Baghdad. Pages:79-83
Rasheed, H. T. , K. J. K. Luti, and M. A. Alaubydi, 2020. A probiotic application of Lactobacillus acidophilus HT1 for the treatment of some skin pathogens. Iraqi Journal of Agricultural Sciences, 51(6):1559-1571. https://doi.org/10.36103/ijas.v51i6.1183
Santagati, M.; S. Marina; P. Francesco; A. Caterina and S. Stefania, 2012. Bacteriocin- producing oral Streptococci and inhibition of respiratory pathogens. Immunol. and Medical Microbiol J.; 22:167-171.
Shokryazdan, P., C. Sieo, R. Kalavathy, J. Liang, N. Alitheen, J Faseleh and Y. Ho., 2014. Probiotic potential of Lactobacillus strains with antimicrobial activity against some human pathogenic strains. Petanika J of Tropical Agricultural Science, 37(1):141-157
Stein, G. E., and M. Gurwith, 1984. Amoxicillin-potassium clavulanate, a beta-lactamase- resistant antibiotic combination. Clinical Pharmacy; 3(6), 591
Thomas J., P. Melvin, S. James, M. April, C. Shelley, K. Sharon, F. Marcelo, M. Romney, J. Amy, M. Tony, S. Michael, N. Audrey, J. Patricia, and D. Pranita, 2020. Performance Standards for Antimicrobial Susceptibility Testing. 30th edition, pp: 30.
Vinderola CG, JA Reinheimer, 2003. Lactic acid starter and probiotic bacteria: a comparative ‘‘in vitro’’ study of probiotic characteristics and biological barrier resistance. Food Research Int; 36:895–904
Vizoso Pinto MG, C. Franz, U. Schillinger, W. Holzapfel, 2006. Lactobacillus spp. within vitro probiotic properties from human faeces and traditional fermented products. Int J Food Microbiol; 109:205–214
Vlková, E.; V. M. Rada, and J, Šmehilová, 2008. Killer. Auto-aggregation and co-aggregation ability in bifidobacteria and clostridia. Folia Microbiol; 2008, 53, 263–269
Winn W. C., S. Allen; W. Janda, E. Koneman, E., G. Procop, P. Schreckenberger and G. Woods, 2006. Koneman’s color atlas and textbook of diagnostic microbiology.6th ed. Lippincott Williams and Wilkins, Philadelphia, USA. pp:201-203
Yang, P. B., Y. Tian, Q. Wang, W. Cong, 2015. Effect of different types of calcium carbonate on the lactic acid fermentation performance of Lactobacillus lactis. Biochemical Engineering Journal, 98, 38-46
Younes, J. A., M. Van der, H. C., E. Heuvel, H. Busscher and G. Reid, 2012. Adhesion forces and coaggregation between vaginal staphylococci and lactobacilli. PloS one; 7(5), e36917
Yavuz B., A. Belma, and O. Derya, 2007. Factors Influencing Autoaggregation and Aggregation of Lactobacillus delbrueckii subsp. bulgaricus Isolated from Handmade Yogurt. Journal of Food Protection, 70(1), Pages 223–227
Yassin, H.Y., A.K. Melconian, and S.S., Mahmood. 2022. Prevalence of exfoliative toxin genes among clinical isolates of Staphylococcus aureus in Iraq. Iraqi Journal of Agricultural Sciences, 53(2):465-470. https://doi.org/10.36103/ijas.v53i2.1554
Yonis, R.W., K.J. Luti, and G.M., Aziz, 2019. Statistical optimization of chitin bioconversion to produce an effective chitosan in solid state fermentation by Aspergillus flavus. Iraqi Journal of Agricultural Sciences, 50(3):916-927. https://doi.org/10.36103/ijas.v50i3.708
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