THE EFFECT OF HELICOBACTER PYLORI ON INTERLEUKIN-6 IN PATIENTS WITH ISCHEMIC HEART DISEASE
Keywords:IHD, H. pylori-IgG, H. pylori-IgM, IL-6, PCR, 16S rDNA, ELISA, DNA
Several studies have shown that the presence of Helicobacter pylori (H. pylori) may be a risk factor for Ischemic Heart Diseases (IHD). Therefore, it is needed to look into the relation between IHD and H. pylori infection. One hundred Iraqi patients with IHD and 60 healthy Iraqis as a control group participated in the current study. The ELISA assay was used for the measurement of the sera levels for anti- H. pylori IgG, IgM, and Interleukin-6 (IL-6). Also, H. pylori DNA in the human venous blood was investigated by the polymerase chain reaction (PCR) using 16S rDNA primers. Between IHD patients and the control group, H. pylori-IgG and IL-6 mean ± SD were (Patient =1.814±0.08, Control = 0.823±0.09, Patient = 49.73±6.60, Control = 0.21±0.02, respectively), and this results showed a highly significant variation (p < 0.05) in anti-H. pylori IgG and IL-6, while no significant variations (p > 0.05) were found in anti- H. pylori IgM. It was also found that PCR amplification of Helicobacter pylori DNA using 16S rDNA primers was positive in the venous blood of 81 (81%) IHD patients. The current study results proposed that there was a relation between H. pylori and Ischemic Heart Disease.
Abbas, L. M. R., A. J. Hashim and W. Al-Hadban 2020. Identification local isolates of Trichophyton mentagrophytes and detection of keratinase gene using PCR technique. Iraqi Journal of Agricultural Science, 51(6), 1534-1542. https://doi.org/10.36103/ijas.v51i6.1181
Abd Al Kareem, R. M. and W. S. Abd 2020. Impact of EBV on multiple in a sample of Iraqi females: Immunological and molecular study. Iraqi Journal of Science, 61(5): 1008-1015
Abd, W. S. and R. M. A. A. Kareem 2020, December. Impact of EBV on multiple sclerosis in some of the Iraqi males: Immunological and molecular study. In AIP Conference Proceeding, 2290(1): 020023. AIP Publishing LLC
Abd, W.S. and N.S. Abd 2021.Ischemic Heart Disease in correlation with some immunological parameters, the Fifth Scientific Conference for Scientific and Human Studies 2021, AIP conference proceeding. AIP Conference Proceedings 2414, 060005 (2023); https://doi.org/10.1063/5.0116787. Published Online: 13 February 2023
Abd W.S. Incidence of Psoriatic Arthritis in Psoriatic Patients in Some of Iraqi Patients according to some serological Tests. 2019 Journal of Pakistan Association of Dermatologists. 30(1): 72-80. https://www.jpad.com.pk/index.php/jpad/article/view/1304/1350.
Abd W.S. Abd N. S. Celiac and Thyroid Diseases in Some of Iraqi Patients with Juvenile Rheumatoid Arthritis. Indian Journal of Public Health Research & Development. 10(10).2072-2075. https://web.p.ebscohost.com.
Abd-Alrahman, T. Z., R. S. Aboud and S. D. Al-Ahmer 2017. Immunological and molecular detection of Helicobacter pylori in patients clinically diagnosed with chronic urticarial and atopic dermatitis. Current Research in Microbiology and Biotechnology, 5(3), 1082-1087
Abd Mohsen , W.S. 2005. ANA,RF and CRP in patients with some of rheumatic symptoms. Baghdad Science Journal. 2(4),638-644. https://www.iasj.net/iasj/article/67947
Abdullah, D., & Aloubaidy, R. M. . 2022. Genetic Polymorhism of Caspase 8 and 9 in Iraq. Iraqi Journal of Agricultural Science, 53(3),505–514. https://doi.org/10.36103/ijas.v53i3.1558
Afsharpooyan, S. and T. Mohammadian 2019. Seroepidemiology study of Helicobacter pylori infection and its effect on cardiovascular diseases in Karaj, Iran. Monoclonal Antibodies in Immunodiagnosis and Immunotherapy, 38(6), 277-281
Ahmed N.A. S.S. Mahmood, and A.H. Abbass. 2019. A comparative study of some virulence factors and phytogenetic characterization of escherichia.coli isolation causing urinary tract infection and The commensal gut microbiota. Iraqi Journal of Agricultural Sciences, 50(4):1193-1198. https://doi.org/10.36103/ijas.v50i4.763
Atta, R. Z. . and R. M. Aloubaidy. 2022. Genetic polymorphism of asthmain Iraq. Iraqi Journal of Agricultural Science: 53(2):288–296. https://doi.org/10.36103/ijas.v53i2.1536
AL-Jobori, M.B., M. T. AL-Oqaili and E. M. Abdulla 2011. Detection of 16S rRNA gene of Helicobacter pylori in patients with peptic ulcer and gastric carcinoma: molecular and bacteriological study. Egypt. Acad.J. Biology. Sci. 3(1): 95-104
AL-Obeidy, E. S. and B. N. Saeed 2011. H. Pylori infection in Iraqi patients with ischemic heart diseases. Journal of the Faculty of Medicine Baghdad, 53(1), 29-31
EL-Ageery, S. M., N. S. Gouda, I. M Fawzy, A. Bahy-Eldeen and R. Mahmoud 2020. Serological evidence of association between Helicobacter pylori infection and coronary artery disease. African Journal of Clinical and Experimental Microbiology, 21(2), 88-96
Fox, J. G., F. E. Dewhirst, Z. Shen, Y. Feng, N. S. Taylor, B. J. Paster, ... and I. Roa 1998. Hepatic Helicobacter species identified in bile and gallbladder tissue from Chileans with chronic cholecystitis. Gastroenterology, 114(4), 755-763
Furuto, Y., M. Kawamura, J. Yamashita, T. Yoshikawa, A. Namikawa, R. Isshiki, … and Y. Shibuya 2021. Relationship between Helicobacter pylori infection and arteriosclerosis. International Journal of General Medicine, 2021(14): 1533–1540
Ghaleb, R., H. Mansour, M. O. Amer, A. Ismael, A. S. Bhagavathula and A. E. Abd Elrazek 2017. Is there a role of Helicobacter Pylori infection in incidence of acute coronary syndrome?. Int. J. Adv. Biomed, 2(2), 25-33
Ghufran, Q. A. A. and W. S. Abd 2020 Immunological study on some of psoriatic iraqi patients treated with etanercept. International Journal of Psychosocial Rehabilitation, 24
Hasegawa, H., I. Mizoguchi, Y. Chiba, M. Ohashi, M. Xu and T. Yoshimoto 2016. Expanding diversity in molecular structures and functions of the IL-6/IL-12 heterodimeric cytokine family. Frontiers in Immunology, 7, 479
Huang, Y., X. G. FAN, Z. S. TANG, L. Liu, X. F. TIAN and N. Li 2006. Detection of Helicobacter pylori DNA in peripheral blood from patients with peptic ulcer or gastritis. Apmis, 114(12), 851-856
Hussein, A. M., and R. Al Sahlanee. 2022. Serological and molecular evaluation of MRP14 in thyroiditis and Its role in pro-inflammatory chemokines activation. Iraqi Journal of Agricultural Science: 53(6), 1368–1376. https://doi.org/10.36103/ijas.v53i6.1652
Ismael, M. K., M. R. Al-Haleem and R. S. Salman 2015. Evaluation of anti-Helicobacter pylori antibodies in a group of Iraqi patients with atherosclerosis and coronary artery disease. Iraqi Journal of Science, 56(1A), 81-88
Izadi, M., M. Fazel, S. H. Sharubandi, S. H. Saadat, M. M. Farahani, M. H. Nasseri, ... and S. Taheri 2012. Helicobacter species in the atherosclerotic plaques of patients with coronary artery disease. Cardiovascular Pathology, 21(4), 307-311
Jabir, N. R., C. K. Firoz, M. A. Kamal, G. A Damanhouri, M. N. Alama, A. S. Alzahrani, ... and S. Tabrez 2017. Assessment of genetic diversity in IL‐6 and RANTES promoters and their level in Saudi coronary artery disease patients. Journal of Clinical Laboratory Analysis, 31(5), e22092
Jafarzadeh, A., A. Esmaeeli Nadimi, M. Nemati, M. Tahmasbi and P. Ahmadi 2010. Serum concentrations of Helicobacter pylori IgG and the virulence factor CagA in patients with ischaemic heart disease. EMHJ-Eastern Mediterranean Health Journal, 16 (10), 1039-1044, 2010
Jamkhande, P. G., S. G. Gattani and S. A. Farhat 2016. Helicobacter pylori and cardiovascular complications: a mechanism based review on role of Helicobacter pylori in cardiovascular diseases. Integrative medicine research, 5(4), 244-249
Jha, H. C., J. Prasad and A. Mittal 2008. High immunoglobulin A seropositivity for combined Chlamydia pneumoniae, Helicobacter pylori infection, and high-sensitivity C-reactive protein in coronary artery disease patients in India can serve as atherosclerotic marker. Heart and vessels, 23(6), 390-396
Khan, M. A., M. J. Hashim, H. Mustafa, M. Y. Baniyas, S. K. B. M. Al Suwaidi, R. AlKatheeri, … and S. N. A. H. Lootah 2020. Global epidemiology of ischemic heart disease: Results from the global burden of disease study. Cureus, 12(7).
Knuuti, J., W. Wijns, A. Saraste, D. Capodanno, E. Barbato, C. Funck-Brentano, … and J. J. Bax 2020. 2019 ESC Guidelines for the diagnosis and management of chronic coronary syndromes: the Task Force for the diagnosis and management of chronic coronary syndromes of the European Society of Cardiology (ESC). European heart journal, 41(3), 407-477
Mohammed, S. A., D. A. Kadhum and M. K. Abbas 2019. Serologic study to detect a relation between myocardial infraction diseases and Helicobacter pylori infections in Baqubah City, Iraq. Indian Journal of Public Health, 10(8), 101
Morieri, M. L., A. Passaro and G. Zuliani 2017. Interleukin-6 “Trans-Signaling” and ischemic vascular disease: the important role of soluble gp130. Mediators of inflammation, 2017
Nakagawa, H., T. Tamura, Y. Mitsuda, Y. Goto, Y. Kamiya, T. Kondo, … and N. Hamajima 2013. Significant association between serum interleukin-6 and Helicobacter pylori antibody levels among H. pylori-positive Japanese adults. Mediators of inflammation, 2013:5
Piko, N., S. Bevc, R. Hojs, F. H. Naji and R. Ekart 2021. The association between pulse wave analysis, carotid-femoral pulse wave velocity and peripheral arterial disease in patients with ischemic heart disease. BMC Cardiovascular Disorders, 21(1), 1-9
Rasmi, Y. and M. H. Seyyed-Mohammadzad 2012. Frequency of Helicobacter pylori and cytotoxine associated gene A antibodies in patients with cardiac syndrome X. Journal of cardiovascular disease research, 3(1), 19-21
Reszka, E., B. Jegier, W. Wasowicz, M. Lelonek, M. Banach and R. Jaszewski 2008. Detection of infectious agents by polymerase chain reaction in human aortic wall. Cardiovascular Pathology, 17(5), 297-302.
Rose-John, S. 2018. Interleukin-6 family cytokines. Cold Spring Harbor perspectives in biology, 10(2), a028415
Saeideh, H. E. Davoud, F. Jalil and R. Jafar 2013. The presence of cagA genome in blood of infected patient with Helicobacter pylorias new marker.J. Bacteriol. Res.5(4).46-50
Sampasa-Kanyinga, H. and R. F. Lewis 2015. Frequent use of social networking sites is associated with poor psychological functioning among children and adolescents. Cyberpsychology, Behavior, and Social Networking, 18(7), 380-385
Tabarek A. A., and M. A. Reema. 2023. Genetic Polymorphisms of HLA-G Gene in Rheumatoid Arthritis. Iraqi Journal of Agricultural Science, 54(2), 378–387. https://doi.org/10.36103/ijas.v54i2.1712
Tomas, M. J., P. M. Baluyot, A. L. D. Santos, J. Faustino, E. Licudine, E. M. Mendoza, … & M. J. Tiburcio 2015. Interleukin 6 as a biomarker of ischemic heart disease. International Journal of Scientific and Research Publications, 5(7), 1-4
Villar-Fincheira, P., F. Sanhueza-Olivares, I. Norambuena-Soto, N. Cancino-Arenas, F. Hernandez-Vargas, R. Troncoso, ... and M. Chiong, 2021. Role of Interleukin-6 in vascular health and disease. Frontiers in Molecular Biosciences, 8, 79
Viswanath, V., S.R. Padmaraj and D. Roy 2015. Genetic instabilities in H. pylori infected cardiovascular diseases. International Journal of Scientific & Engineering Research, 6
Wang, B., P. Li, F. He, Y. Sha, X. Wan and L. Wang 2021. Spatiotemporal variations in ischemic heart disease mortality and related risk factors in China between 2010 and 2015: a multilevel analysis. BMC Public Health, 21(1), 1-10
Yakoob, J., S. Abid, W. Jafri, Z. Abbas, M. Islam, and Z. Ahmad 2006. Comparison of biopsy-based methods for the detection of Helicobacter pylori infection. British Journal of Biomedical Science, 63(4), 159-162
Yildirim, Z., B. Bozkurt, D. Ozol, F. Armutcu, R. Akgedik, H. Karamanli, ... and M. İkizek 2016. Increased exhaled 8‐isoprostane and interleukin‐6 in patients with Helicobacter pylori infection. Helicobacter, 21(5), 389-394.
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