SYNERGISTIC EFFECT OF ANTIBIOTIC WITH GREEN SYNTHESIZED SILVER NANO PARTICLES AGIANST UROPATHOGENIC E.COLI BIOFILM
DOI:
https://doi.org/10.36103/ijas.v54i6.1862Keywords:
antibiofilm, silver nanoparticles, optimization, synergistic, maca roots.Abstract
The bacterial ability to form biofilm had been complicated the infection healing process, due to antibiotic resistance. Urinary tract infection is an optimal example of such infections that caused by bacterial biofilms. So the current study aimed to find an alternative method to inhibit the uropathogenic bacteria using nanotechnology. Uropathogenic Escherichia coli (UPEC) was isolated from 110 clinical samples. These bacterial isolated were tested for their ability to form biofilm by two methods: Microtiter plate and congo red agar. Antibiotic susceptibility was detected to determine the multidrug-resistant isolates. An ecofriendly green method was depended for the synthesis of silver nanoparticles (AgNPs) using alcoholic extract of Lepidium meyenii yellow root. Silver nanoparticles were characterized by several techniques, and it was detected with a diameter of 44.89 nm. Anti-biofilm activity of AgNPs alone and with antibiotic was detected and the Scanning electron microscopy observations clearly indicated that 7.1825 mg/ml AgNPs prevented the biofilm formation. This is the first worldwide evidence-based research study about the biosynthesis of silver nanoparticles from alcoholic yellow root extract of plant Lepidium meyenii and use it against UPEC biofilm.
References
Al-Jubouri, A. K., N. H. Al-Saadi, and M. A. Kadhim. 2022. Anti-inflammatory and anti-bacterial activity of copper nanoparticles synthesized from Myrtus communis leaves extract. Iraqi Journal of Agricultural Sciences 53 (3):698-711. https://doi.org/10.36103/ijas.v53i3.1580
Al-Khafaji, M.H.M., S.M.A. Majeed, and R.Q., Basi, 2017. Biosynthesis of silver nanoparticles by food-origin E.coli and candida species and testing its antimicrobial activity against pathogenic bacteria and fungi. IOSR J. Pharm. Biol. Sci, 12,: 29-34.
Al-Khafaji, M.H.M., H.K. Tawfeeq, and B.A., Mahdii, 2016. Effect of combination of d-glycin and antibiotics on biofilm. World J Exp Biosci 4: 112-117
Al-taee, E. H. 2020. Effect of silver nanoparticles synthesized using leaves extract of olive on histopathological and cytogenetic effects in albino mice. Iraqi Journal of Agricultural Sciences. 51 (5):1448-57. https://doi.org/10.36103/ijas.v51i5.1155
Anantharama, N.A., K.S. Sheethal, K.S. and G., Mary, 2016. Green synthesis and its applications of magnesium oxide nanoparticles from the seeds of Lepedium sativum. International Journal of Recent Scientific Research, 7,: 14029-14032.
Ansari, M.A., H.M., Khan, A.A., Khan, S.S. Cameotra, and R., Pal, 2014. Antibiofilm efficacy of silver nanoparticles against biofilm of extended spectrum β-lactamase isolates of Escherichia coli and Klebsiella pneumo-niae. Applied Nanoscience, 4, pp.859-868.
Aslam, B., W., Wang, M. I., Arshad, M., Khurshid, S., Muzammil, M. H., Rasool, M. A., Nisar, R. F., Alvi, M. A., Aslam, M. U. Qamar, and M. K. F., Salamat, 2018. Antibiotic resistance: a rundown of a global crisis. Infection and drug resistance, pp.1645-1658.
Atwan, Q. S. and Hayder, N. H., 2020. Eco-friendly synthesis of Silver nanoparticles by using green method: Improved interaction and application in vitro and in vivo. The Iraqi Journal of Agricultural Science, 1(Special Issue):201-216 https://doi.org/10.36103/ijas.v51iSpecial.898
Barapatre, A., K.R. Aadil, and H., Jha, 2016. Synergistic antibacterial and antibiofilm activity of silver nanoparticles biosynthesized by lignin-degrading fungus. Bioresources and Bioprocessing, 3; 1-13.
Beyene, H. D., A. A., Werkneh, H. K. Bezabh, and T. G., Ambaye, 2017. Synthesis paradigm and applications of silver nanoparticles (AgNPs), a review. Sustainable Materials and Technologies, 13; 18-23.
Bloukh, S.H., Z., Edis, H.A. Sara, and M.A., Alhamaidah, 2021. Antimicrobial properties of Lepidium sativum L. facilitated silver nanoparticles. Pharmaceutics, 13(9).
Coffey, B.M. and G.G., Anderson, 2014. Biofilm formation in the 96-well microtiter plate. Pseudomonas Methods and Protocols, pp.631-641
Freeman, D. J., F. R., Falkiner, and C. T. Keane, 1989. New method for detecting slime production by coagulase negative staphylococci. Journal of Clinical Pathology, 42(8): 872-874
Funari, R. and A.Q., Shen, 2022. Detection and characterization of bacterial biofilms and biofilm-based sensors. ACS Sensors, 7(2), pp.347-357
Galatage, S.T., A.S., Hebalkar, S.V., Dhobale, O.R., Mali, P.S., Kumbhar, S.V. Nikade, and S.G., Killedar, 2021. Silver nanoparticles: properties, synthesis, character-rization, applications and future trends. Silver Micro-Nanoparticles Prop. Synth. Charact. Appl. IntechOpen.
doi: 10.5772/intechopen.99173
Hassan A, J, Usman F, Kaleem M, Omair A, Khalid and M. Iqbal. 2011. Evaluation of different detection methods of biofilm formation in the clinical isolates. Braz J Infect Dis; 15:305-11
Ibrahim, O.M.S., A.M. Abed, and N.Z., Yahea, 2020. Evaluation of biological activity of greenly synthesized silver nanoparticles using aloe vera gel extract as antibacterial agent In-vitro and In-vivo. Iraqi Journal of Agricultural Science, 51(6):1706-1715. https://doi.org/10.36103/ijas.v51i6.1199
Kader, A.A. and A.K., Kumar, 2004. Prevalence of extended spectrum beta-lactamase among multidrug resistant gram-negative isolates from a general hospital in Saudi Arabia. Saudi Medical Journal, 25(5), pp.570-574
Khatoon, Z., C.D., McTiernan, E.J., Suuronen, T.F. Mah, and E.I., Alarcon, 2018. Bacterial biofilm formation on implantable devices and approaches to its treatment and prevention. Heliyon, 4(12).
Krishnan, R., V. Arumugam, and S.K., Vasaviah, 2015. The MIC and MBC of silver nanoparticles against Enterococcus faecalis-a facultative anaerobe. J Nanomed Nanotec-nol, 6(3), p.285
Logeswari, P., S. Silambarasan, and J., Abraham, 2015. Synthesis of silver nanoparticles using plants extract and analysis of their antimicrobial property. Journal of Saudi Chemical Society, 19(3), 311-317
Mahmudin, L., E., Suharyadi, A.B.S. Utomo, and K., Abraha, 2015. Optical properties of silver nanoparticles for surface plasmon resonance (SPR)-based biosensor applications. Journal of Modern Physics, 6(08), p.1071
Okafor, F., A., Janen, T., Kukhtareva, V. Edwards, and M., Curley, 2013. Green synthesis of silver nanoparticles, their characterization, application and antibacterial activity. International Journal of Environmental Research and Public Health, 10(10), pp.5221-5238
Panda, P.S., U. Chaudhary, and S.K., Dube, 2016. Comparison of four different methods for detection of biofilm formation by uropathogens. Indian Journal of Pathology and Microbiology, 59(2), p.177
Prabhu, S. and E.K., Poulose, 2012. Silver nanoparticles: mechanism of antimicrobial action, synthesis, medical applications, and toxicity effects. International Nano Lette-rs, 2,.1-10
Raeispour, M. and R., Ranjbar, 2018. Antibiotic resistance, virulence factors and genotyping of Uropathogenic Escherichia coli strains. Antimicrobial Resistance & Infection Control, 7(1), pp.1-9
Rafique, M., I., Sadaf, M.S. Rafique, and M.B., Tahir, 2017. A review on green synthesis of silver nanoparticles and their applications. Artificial Cells, Nanomedicine, and Biotechnology, 45(7), pp.1272-1291
Saleh, G.M., S.A., Alash, H.Y. Fadil, and H.B., Ali 2020. The effect of termites extract on inhibition of growth of some pathogenic bacteria and synthesis of biofilm. Iraqi Journal of Agricultural Sciences. 51(Special Issue): 176-183. https://doi.org/10.36103/ijas.v51iSpecial.895
Schulze, A., F., Mitterer, J.P. Pombo, and S., Schild, 2021. Biofilms by bacterial human pathogens: Clinical relevance-development, composition and regulation-therapeutical strategies. Microbial Cell, 8(2), p.28
Shanmuganathan, R., I., Karuppusamy, M., Saravanan, H., Muthukumar, K., Ponnuchamy, V.S. Ramkumar, and A., Pugazhendhi, 2019. Synthesis of silver nanoparticles and their biomedical applications-a comprehensive review. Current Pharmaceutical Desi-gn, 25(24); 2650-2660
Singh, P., Y.J., Kim, H., Singh, C., Wang, K.H., Hwang, M.E.A. Farh, and D.C., Yang, 2015. Biosynthesis, characterization, and antimicrobial applications of silver nanoparticles. International Journal of Nanomedicine; 2567-2577
Stehfest, K., J. Toepel, and C., Wilhelm, 2005. The application of micro-FTIR spectroscopy to analyze nutrient stress-related changes in biomass composition of phytoplankton algae. Plant Physiology and Biochemistry, 43(7); 717-726
Suhail, R. N., S. A. Mustafa, and E. E. AL-Obodi. 2022. Effeciency of silver nanopart-icales as antibacterial against Aeromonas hydrophila isolated from infected common carp. Iraqi Journal of Agricultural Sciences. 53(3):589-597. https://doi.org/10.36103/ijas.v53i3.1568
Vestby, L.K., T., Grønseth, R. Simm, and L.L., Nesse, 2020. Bacterial biofilm and its role in the pathogenesis of disea-se. Antibiotics, 9(2), p.59
Vranic, S.M. and A., Uzunovic, 2016. Antimicrobial resistance of Escherichia coli strains isolated from urine at outpatient population: a single laboratory experie-nce. Materia Socio-medica, 28(2), p.121
Zhang, X., S., Yan, R.D. Tyagi, and R.Y., Surampalli, 2011. Synthesis of nanoparticles by microorganisms and their application in enhancing microbiological reaction rates. Chemosphere, 82(4): pp.489-494.
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