ISOLATION, IDENTEFECATION AND EFFECINCY OF PSEUDOMONAS FLUORESCENS BACTERIA TO TERMITE MICROCEROTERMIS DIVERSUS
DOI:
https://doi.org/10.36103/ijas.v54i6.1859Keywords:
White ant , biocontrol , Iraqi environment , LocustAbstract
This study was conducted to isolate the bacteria Pseudomonase fluorescens from the termite, locust, and American cockroach in the Iraqi environment and to diagnose it based on morphological, biochemical, and molecular diagnosis using the polymerase chain reaction (PCR), as well as test its pathogenicity and efficacy to termites under laboratory conditions. The results of morphological , biochemical, and molecular diagnosis using polymerase chain reaction (PCR) tests showed the isolated bacterial isolates are similar to P. fluorescens .The results of efficiency of different isolates of P. fluorescens showed that they have a high pathogenicity towards termite workers in laboratory and incubation condition.
References
Abbott , W.S. 1925.A method of computing the effectiveness of an insecticide . J. Econ .Entom., 1 ( 18):265- 267
Abed ,Y.M., Abdul-Ratha ,H. A. and H.A Hadown. 2016. Effect of biofertilizer produced from local isolate of Pseudomonas putida and Pseudomonas fluorescens on some soil characteristics and yield of wheat (Triticum aestivum L) A – yield components , Iraqi Journal of Agricultural Sciences, 47(6):1404:1412. https://doi.org/10.36103/ijas.v47i6.468
Abed ,Y.M., H. A Abdul-Ratha and H.A. Hadown, 2016. Effect of biofertilizer produced from local isolate of Pseudomonas putida and Pseudomonas fluorescens on some soil characteristics and yield of wheat (Triticum aestivum L) B -Concentrations of some nutrients in soil. Iraqi Journal of Agricultural Sciences, 47(6):1413-1422. https://doi.org/10.36103/ijas.v47i6.470
Arab, A. , A. M. Costa- Leonardo, F. E. Casarin, A.C. Guaraldo and R. C. Chaves .2005. Foraging activity and demographic patterns of two termite species (Isoptera: Rhinotermitidae) living in urban landscapes in Southeastern Brazil. Eur. J. Entomol., 102(169): 691- 697.
Al-Shammari, H.I., H.K. Al-Zubaidy, 2017. Numerical response and effecincy of conversion of ingested food of predetor Dicrodiplosis manihoti Harris, (Diptera : Cecidomyidae ) for eggs densities of mealy bug Planococcus citri (Risso), (Hemiptera : Pseudococcidae) . Iraqi Journal of Agricultural Sciences . 48, (2):496-500. . https://doi.org/10.36103/ijas.v48i2.412
Al-waily ,D.S., L. A. Al-saad, and S. S. Al-dery. 2018. Formulation of Pseudomonas fluorescens as biopesticides agents soil born root pathogens. Iraqi Journal of Agricultural Sciences, 49(2):235-242. https://doi.org/10.36103/ijas.v49i2.227
Al-Zubaidy, H.K., and H.I. Al-Shammari, 2017. Growth threshold and degree days requirement for development and growth of citrus mealy bug Planococcus citri (Risso), Hemiptera : Pseudococcidae. Iraqi Journal of Agricultural Sciences 48 (2):501-506. https://doi.org/10.36103/ijas.v48i2.415
.Bontemps ,C, G., G. Golfier , , C. Cris – liebe , C. Sébastien , T. Talini and C. Boivin – Masson .2005. Microarray-based detection and typing of the rhizobium nodulation gene nodc: potential of DNA arrays to diagnose biological functions of interest . Journal Applied and Environmental Microbiology, 71 (12): 8042-8048.
Devi, K. K. , N. Seth, S. Kothamasi and D. Kothamasi. 2007. Hydrogen cyanide producing rhizobacteria kill subterranean termite Odontotermes obesus (Rambur) by cyanide poisoning under in-vitro conditions. Curr. Microbiol. , 54 (1) : 74–78
Devi, K. K. and D. Kothamasi .2009. Pseudomonas fluorescens CHA0 can kill subterranean termite Odontotermes obesus by inhibiting cytochrome c oxidase of the termite respiratory chain. FEMS Microbiol. Lett., 301(1): 195–200
Esmail,T.N., H.K. Shekhany, , F.M.Faraj, and S.A. Mustafa. 2019. Food Preference of Termite (Microcerotermes diversus Silv.) for Forest Trees in Erbil Governorate. Tikrit Journal for Agricultural Sciences ,19 (3):72-79
Fatima, S. and Anjum, T. 2017. Identification of a potential ISR determinant from Pseudomonas aeruginosa PM12 against Fusarium wilt in tomato. Frontiers in plant science, 8: 848.
Govorushko , S . 2019. Economic and ecological importance of termites: A global review . Entomol. Sci. , 22: 21–35.
Grimont, F. and P.A.D. Grimont. 2005 .Genus XXXXIV Serratia Bizio .In G.B.Garrity (Ed) , Bergey᾽s Manual of Systematic Bacteriology , the Gamma proteobacteria ) New : Springe , pp: 799-811.
Iskender , N.A. , O.F. Algur , Y. Aksu and A. Saral. 2017. Isolation, identification and characterization of biotechnologically important bacteria from microflora of Dryocosmus kuriphilus Yasumatsu (Hymenoptera: Cynipidae) . Biotechnology & Biotechnological Equipment , 31(3):505-510.
Sakthivel, U., S.Mahalakshmi, & B.Karthikeyan, 2009. Studies on isolation and characterization and its (Pseudomonas fluorescens) on yield of tomato. Methods. 1(1): 33–39.
Khan , K. I. , R. H. Jafri and A. Muzaffar. 2008 . Discovery and pathogenicity of Pseudomonas fluorescens against various species of termites. Punjab University Joumal of Zoology , 23(2):47-57..
Lacey , L.A. 2012 . Manual of Techniques in Invertebrate Pathology. 2nd ed., Elsevier Ltd . Printed and bound in great Britain. pp: 484.
Liu , S . , X. Lin, J.E. Behm ,H. Yuan , P. Stiblik , J. Sobotník , J .Gan, , S. Xia and X. Yang . 2019. Comparative responses of termite functional and taxonomic diversity to landuse change. Ecol. Entomol. , 44: 762–770
Luca R. .2020. Plant-Growth-Promoting Bacteria (PGPB) against Insects and Other Agricultural Pests. J. Agronomy , 10:1-12.
Manasa, K. , R. Subhash and S. Triveni .2017. .Isolation and characterisation of Pseudomonas fluorescens isolates from different rhizosphere soils of Telangana. Journal of Pharmacognosy and Phytochemistry, 6(3): 224-229.
Mohandkaci , H. O. , S. Khemili, F. Benzena and F. Halouane .2015. Isolation and identification of entomopathogenic bacteria from Algerian desert soil and their effects against migratory locust, Locusta migratoria (L.). Egyptian J . Biol. Pest Control., 25(3):739-746
Morrison, C. K., Arseneault, T., Novinscak, A. and M. Filion. 2016. Phenazine-1-carboxylic acid production by Pseudomonas fluorescens LBUM636 alters Phytophthora infestans growth and late blight development. Phytopathology, 107(3): 273-279.
Nepali, B., S. Bhattarai, and J. Shrestha. 2018. Identification of Pseudomonas fluorescens using different biochemical tests. International Journal of Applied Biology, 2(2): 27-32.
Oni, F.E., N .Geudens,. A.Adiobo, O.O.Omoboye, E.A.Enow, J.T. Onyeka, A.E. Salami, R.De De Mot, J.C.Martins, and M.Höfte. 2020. Biosynthesis and Antimicrobial Activity of Pseudodesmin and Viscosinamide Cyclic Lipopeptides Produced by Pseudomonads Associated with the Cocoyam Rhizosphere. Microorganisms 2020, 8, 1079.
Oni, F.E.; Q. Esmaeel,; J.T.Onyeka,; R.Adeleke, C. Jacquard, C. Clement, H. Gross, E.Ait Barka, and M. Höfte. 2022. Pseudomonas Lipopeptide-Mediated Biocontrol: Chemotaxonomy and Biological Activity. Molecules 2022, 27, 372. https://doi.org/10.3390/ molecules27020372
Othman ,B. A. and E. S. Kakey . 2021. Pesticides bioacumalation and their soil pollutant effect. Iraqi Journal of Agricultural Sciences, 52(1):36-47. https://doi.org/10.36103/ijas.v52i1.1234
Otsu , Y. , Y. Matsuda , H. Mori , H. Ueki , T. Nakajima and K. Fujiwara .2004. Stable phylloplane colonization by entomopathogenic bacterium Pseudomonas fluorescens KPM-018P and biological control of phytophagous ladybird beetles Epilachna vigintioctopunctata (Coleoptera: Coccinellidae). Biocontrol Sci. Technol., 14 (5) : 427–439
Qessqoui , R. , R. Bouharroud , A. Amarraque , A. Ajerrar ,E. Mayad , B. Chebli , M. Dadi , R. Elaini , F. Elfilali and A.S. Walters. 2017. Ecological applications of Pseudomonas as a biopesticide to control two-spotted mite Tetranychus urticae: chitinase and HCN production. Journal of Plant Protection Research, (57) 4: 409–416
Reddy, P. P. 2014. Plant Growth Promoting Rhizobacteria for HorticulturalCrop Protection .Us: Springer, pp : 330.
Rust, M. K. and N. Y. Su 2012. Managing social insects of urban importance. Annu. Rev. Entomol.,57: 355- 375
Saleh ,G.M. , S.A.Alash, H.Y.Fadil and H.B.Ali. 2020. The effect of termites extract on inhibition of growth of some pathogenic bacteria and synthesis of biofilm. Iraqi Journal of Agricultural Sciences,51(Special Issue):176-183. https://doi.org/10.36103/ijas.v51iSpecial.895
Scales, B. S., Dickson, R. P., Lipuma, J. J. and G. B. Huffnagle,. 2014. Microbiology, genomics, and clinical significance of the Pseudomonas fluorescens species complex, an unappreciated colonizer of humans. Clinical Microbiology Reviews. 27(4): 927–948.
Shareiji, I. H. .2019. Antagonism between the pathogenic bacteria Bacillus thuringiensis and the types of symbiont bacteria in the stomach of the terrestrial Microcerotermes diversus Silv and its effect on causing death to the members of the land. M.Sc. Thesis . faculty of Agriculture . Baghdad University. 138 pp..
Suman, B. , A. Vijaya Gopal ,R. Subhash and S. Triveni. 2015. Isolation and characterization of native Pseudomonas fluorescens isolates from rangareddy district telangana . progressive research- An International Journal Society for Scientific Development, 10 : 1865-1868
Vega, F. and H. Kaya .2011. Insect pathology. 2nd ed. .Academic Press .USA. pp: 508.
Willcox, M. D. 2007. Pseudomonas aeruginosa infection and inflammation during contact lens wear: a review. Optometry and Vision Science : Official Publication of the American Academy of Optometry, 84(4): 273¬278
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