MOLECULAR DETECTION OF NEW STREPTOMYCES SPP. FROM IRAQI OIL CONTAMINATED SOIL
Keywords:Isolate, Antibacterial, Antiviral, genetic sequences
Streptomyces spp. is one of Actinomycete that produce variety of secondary metabolite (antibacterial, antifungal and anticancer product). Bacteria that used in this study was isolated from oily soil in Baghdad /Iraq. The morphological characters was gram positive, branching, spore former. The goal of this research was to find genetic polymorphisms of one bacterial isolate (assigned S1) and to assess the accurate phylogenetic distribution of this isolated based on ribosomal sequences. In this work one genomic region was amplified that covered a portion of the 16S rRNA sequences. According to the identified results, the currently investigated sample was positioned within the major phylogenetic clade of the Streptomyces albidoflavus sequences in the generated comprehensive tree. In conclusion, the present tree provided an inclusive tool for the confirmed identity of the sample under investigation As a result, the PCR-sequencing approach was used in the studied sample has presented a confirmed identity of this sample and showed the pattern of its phylogenetic distribution. This sort of observed diversity may suggest the possibility to utilize the current 16S rRNA sequences in the accurate detection and discrimination for the Streptomyces albidoflavus sequences.
Al-Abedi G.J.K. and Al-Amery M.A.M. molecular diagnosis and phylogenetic analysis of BABESIA spices isolated from ticks of infested cattle in Wasit governorate, Iraq. Iraqi Journal of Agricultural Sciences. 52(1):136-145. https://doi.org/10.36103/ijas.v52i1.1245
Ali, M., F. Abbas, and T. Mushtaq., 2021. Association of TGF-β2 gene polymorphism with Growth rate in local chickens. The Iraqi J. Vet. Med., 45(1): 9-16
Al-Obaidi R.M., Salih G.F. and Nore B.F.2021.bioactivity characterization of purified recombinant hypothetical protein coded by open reading FRAME-112 of STREPTOMYCES. Iraqi Journal of Agricultural Sciences. 52(2):502-511. https://doi.org/10.36103/ijas.v52i2.1314
Chater, K. F. 1993. Genetics of differentiation in Streptomyces.Annu Rev. Microbiol. 47: 685-713
Ensign, J. C. 1978. Formation, properties and germination of actinomycete spores. Annu Rev Microbiol32, 185-219
Gupta, R. S. 1999. What are Archaebacteria: Life’s Third Domain or Monoderm Prokaryotes Related to Gram-positive Bacteria? A new Proposal for the Classification of Prokaryotic Organisms. Molecular Microbiology. 29(3):695-707
Hashim, H.O., M.K. Mohammed, M.J. Mousa, HH. Abdulameer, AT. Abdulameer, S.A. Hassan and M.B. Al-Shuhaib. 2020. Infection with different strains of SARS-CoV-2 in patients with COVID-19. Archives of Biological Sciences. 25:72(4):585
Hassan, S.A. and M.T.S. Al-Khateeb, 2017. Biological pre –treatment use local wild strain of LIGNOLYTIC filamentous bacteria to improve in vitro dry matter digestibility and reduction lignin content of low quality roughages. Iraqi Journal of Agricultural science. 48: 6-11 (Special). https://doi.org/10.36103/ijas.v48iSpecial.238 9. Horinouchi, S. and T. Beppu. 1990. Autoregulatory factors of secondary metabolism and morphogenesis in actinomycetes. Crit. Rev. Biotechnol. 10: 191-204
Hussein S.I., Aziz G.M., Shanshal R.M. and Ghani A.L. 2018. Determination the optimum conditions of lactase production from local isolate of STREPTOMYCES SP. using sold state fermentation. Iraqi Journal of Agricultural Sciences. 49(4):685-693. https://doi.org/10.36103/ijas.v49i4.79
Khucharoenphaisan, K., N. Sripairoj and K. Sinma. 2012. Isolation and identification of actinomycetes from termite's gut against human pathogen. Asian J.Anim.Vet.Adv.,7:68-73
Kuster E. 1968. Taxonomy of soil Actinomycetes & Related Organisms. In : " Ecology of Soil Bacteria", eds. Gray (TRG) and Parkinson (D.), 322-336. Liverpool University Press, Liverpool
Lee, J.Y. and B.K. Hwang. 2002. Diversity of antifungal actinomycetes in various vegetative soils of Korea. Can. J. Microbiol. 48: 417
Letunic, I and P. Bork, 2019. Interactive tree of life (iTOL) v4: recentupdates and new developments. Nucleic Acids Res. 2:47(W1): W256-W259.
Miles, R.S., J.G. Collee., B.P. Marion., A.G. Fraser., A. Simmons. and B. Watt. 1996. test for identification of bacteria in Mackie and McCartney Practical Medical Microbiology (14th ed.): Churchill-Livingstone; p: 133-150
Nadhom, B. N. 2018. Study of molecular composition of virulence bacteria isolated from bovine mastitis with biofilim production, Iraqi Journal of Agricultural Sciences . 49(5):840-846. https://doi.org/10.36103/ijas.v49i5.44
Nascimento, T. P., Sales, A. E., Porto, C. S., Brandão, R. M. P., Takaki, G. M. C., Teixeira, J. A. C., ... & Porto, A. L. F. .2015. Production and characterization of new fibrinolytic protease from Mucor subtillissimus UCP 1262 in solid-state fermentation. Advances in Enzyme Research, 3(03), 81-91
Niladevi b, K. N. and P. Prema, 2008. Immobilization of laccase from Streptomyces psammoticus and its application in phenol removal using packed bed reactor. World Journal of Microbiology and Biotechnology. 24:1215-1222. Iraqi J. of Agricultural Sciences –8201:49(4):685-693
Niladevi, K.N., R.K. Sukumaran, and P. Prema, 2007. Utilization of rice straw for laccase production by Streptomyces Psammoticus in solid-state fermentation. Indian Journal of Microbiology and Biotechnology. 34 (10):665-74. Iraqi J. of Agricultural Sciences –8201:49(4):685-693
Rakesh, K.N., N., Dileep, S. Junide, and T. R. Prashith Kekuda, 2014. Optimization of culture conditions for production of antimicrobial metabolites by bioactive Streptomyces species SRDP-TK-07. An international Journal of Advances in Pharmaceutical science. 5 (1): 1809 -1816. The Iraqi J. of Agricultural Sciences –8201:49(4):685-693
Sievers, F. and D. G. Higgins. 2014. "Clustal Omega, accurate Alignment of Very Large Numbers of Sequences." In Multiple Sequence alignment Methods, pp. 105-116. Humana Press, Totowa, N.J.
Smit, G., M. H. Sraver, B. Lugtenberg, and J. W. Kijne. 1992. Flocculance of Saccharomyces cerevisiae cells induced by nutrient limitation, with cell surface hydrophobicity as a major determinant. Appl. Environ. Microbiol.58: 3709-3714
William, F. and R.J. Paul. 2006. Developing a new resource for drug discovery: Marine actinomycete bacteria. Nat. Chem. Biol., 2: 666-673
Zhang Z, S. Schwartz, L. Wagner and W. Miller. 2000. A greedy algorithm for aligning DNA sequences. J. Comput. Biol. 7(1-2):203-14.
Zheng, Z., W. Zeng, Y. Huang, Z .Yang, J. Li, H. Cai and W. Su. 2000. Detection of antitumor and antimicrobial activities in marine organism associated actinomycetes isolated from the Taiwan Strait, China. FEMS Microbiol. Lett. 188:87-9 1.
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