• Mohammed Fadhil Taleb
  • A. H. Ulaiwi



pancreas activity, cholesterol, triglycerides, recombinant and classical vaccine.


This study was aimed to determine whether chromium (Cr III) supplements have a beneficial effect on the insulin secretion and lipid profile of layers vaccinated against H5 avian influenza (AI). The study included serological parameter by ELISA kit and biochemical parameter for lipid profile test as well as to compare between highly pathogenic avian influenza (HPAI) recombinant (rH5N1) vaccine and classical (cH5N8) vaccine. The study was designed as follow: {G1: given rH5N1 vaccine only; G2: given cH5N8 only; G3: given rH5N1 with Cr III supplement; G4: given cH5N8 with Cr III supplement; G5: given Cr III supplement; G6: is negative control with no vaccination or supplementation}. Levels of insuline at 21 days old there were showed a significant increase in G3 and G5 as compared with another groups. At 42 days old, G3, G4 and G5 revealed the highest levels as compared with another groups. Similarity results for 60 days old groups. The results of lipid profile test at 60 days old showed the lowest level of cholesterol in G5 as compared to another groups and lowest levels of vLDL in G3, G4 and G5. However, there were no significant differences in triglycerides (Tri.Gs), HDL and LDL levels in all groups. In conclusion, Cr III supplementation can improve insulin secretion in layers vaccinated with AI H5 vaccines and it has a positive impact on lipid profile.


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Akbari, M. and M. Torki. 2013. Effects of dietary chromium picolinate and peppermint essential oil on growth performance and blood biochemical parameters of broiler chicks reared under heat stress conditions. Int. J. Biometeorol. 58(6): 1383-1391.

Alani, G. A. and A. A. Albadri. 2021. Reasons related to the deterioration of poultry industry for broiers in the middle and southern region of Iraq. Iraqi Journal of Agricultural Sciences. 52(2): 429-436.

Alders, R., J. A. Awuni, B. Bagnol, P. Farrell and N. de Haan. 2013. Impact of avian influenza on village poultry production globally. EcoHealth. 11(1): 63-72.

Alkaissy G. A. 2007. A comparative between, enzyme and yeast chromium in the feel on the intestinal microflora balance in broiler chicks. Iraqi J. Vet. Med. 31(1): 104-112.

Allawe, A. B. 2008. Study of effect of immunosuppresion caused by H9N2 in broiler. Iraqi J. Vet. Med. 32(2): 80-89.

Aufi, I. M., H. Y. Fadhil, A.W. Ali, F. G. Alhamdani and F. M. Owaid. 2020. Epidemiology of seasonal influenza outbreak among Iraqi population:2020. Iraqi Journal of Agricultural Sciences. 51(1): 454-461.

Capua, I. and D. J. Alexander. 2008. Ecology, epidemiology and human health implications of avian influenza virus infections. In Capua I. and D. J. Alexander (eds). Avian influenza and Newcastle disease: A Field and Laboratory Manual. Springer. pp:1-18.

Habibi, H. and N. Ghahtan. (2019). Effects of medicinal plant powder as feed additives on growth performance, carcass characteristics, and immune response of Coturnix japonica against avian influenza and Newcastle disease vaccine virus. Comp. Clin. Pathol. 28(6): 1581-1588.

Habibian, M., S. Ghazi, and M. M. Moeini. 2013. lack of effect of dietary chromium supplementation on growth performance and serum insulin, glucose, and lipoprotein levels in broilers reared under heat stress condition. Biol. Trace Elem. Res. 153(1): 205-211.

Hashem, S.H. and M.J. Alsaadi. 2019. Chromnium piclonat effects in diet of male rabbits exposed to heat stress on some productive traits and blood serum parameters. Iraqi Journal of Agricultural Sciences. 50(4): 1100-1108.

Jahanian, R. and E. Rasouli. 2015. Dietary chromium methionine supplementation could alleviate immunosuppressive effects of heat stress in broiler chicks. J. Anim. Sci. 93(7): 3355-3363.

Joody, A. A., I. A. Abdulhassan and D. H. Alhassani. 2018. Association of ghrelin gene polymorphisms and expression levels with some biochemical traits in broiler chickens. Iraqi Journal of Agricultural Sciences. 49(5): 271-278.

Khamas, E. J. 2008. Avian influenza (H9N2) outbreak in Iraq. Iraqi J. Vet. Med. 32(1): 223-230.

Lee, D.N., F. Y. Wu, Y. H. Cheng, R. S. Lin and P. C. Wu. 2003. Effects of dietary chromium picolinate supplementation on growth performance and immune responses of broilers. Asian-Australas. J. Anim. Sci. 16(2): 227-233.

Lindh, U. 2012. Biological functions of the elements. In Selinus O. (ed). Essentials of medical geology. Springer. pp: 129-177.

Lu, J. W., J. P. McMurtry and C. N. Coon. 2007. Developmental changes of plasma insulin, glucagon, insulin-like growth factors, thyroid hormones, and glucose concentrations in chick embryos and hatched chicks. Poult. Sci. 86(4): 673-683.

Marmett, B. and R. B. Nunes. 2016. Effects of chromium picolinate supplementation on control of metabolic variables: a systematic review. J. Food Nutr. Res. 4(10): 633-639.

Mir, N. A., P. K. Tyagi, A. K. Biswas, A. B. Mandal, S. A. Sheikh, C. Deo, D. Sharma, and A. K. Verma. 2017. Impact of feeding chromium supplemented flaxseed based diet on fatty acid profile, oxidative stability and other functional properties of broiler chicken meat. J. Food Sci. Technol. 54(12): 3899-3907.

Moeini, M. M., A. Bahrami, S. Ghazi and M. R. Targhibi. 2011. The effect of different levels of organic and inorganic chromium supplementation on production performance, carcass traits and some blood parameters of broiler chicken under heat stress condition. Biol. Trace Elem. Res. 144(1): 715-724.

Moghetti, P. and F. Tosi, 2020. Insulin resistance and PCOS: chicken or egg? J Endocrinol Invest. 44(3): 233-244.

Qaid, M. M. and M. M. Abdelrahman. 2016. Role of insulin and other related hormones in energy metabolism - A review. Cogent Food Agric. 2(1): 1-18.

Sahin, K., N. Sahin, M. Onderci, F. Gursu and G. Cikim. 2002. Optimal dietary concentration of chromium for alleviating the effect of heat stress on growth, carcass qualities, and some serum metabolites of broiler chickens. Biol. Trace Elem. Res. 89(1): 53-64.

SAS. 2012. Statistical Analysis System, User's Guide. Statistical. Version 9.1th ed. SAS. Inst. Inc. Cary. N.C. USA.

Swaroop, A., M. Bagchi, H. G. Preuss, S. Zafra-Stone, T. Ahmad and D. Bagchi. 2019. Benefits of chromium(III) complexes in animal and human health. In: Vincent J. B. (ed): The nutritional biochemistry of chromium (III). Elsevier. pp: 251-278.

Toghyani, M., M. Toghyani, M. Shivazad, A. Gheisari and R. Bahadoran. 2011. Chromium supplementation can alleviate the negative effects of heat stress on growth performance, carcass traits, and meat lipid oxidation of broiler chicks without any adverse impacts on blood constituents. Biol. Trace Elem. Res. 146(2): 171-180.

Ulaiwi, A. H. 2019. Role of some aromatic essential oil on immune status against infectious bronichitis vaccine and lipid profile of broiler chicken. Iraqi Journal of Agricultural Sciences. 50(6): 1630-1634.

Ulaiwi, A. H. 2023. Determination of dopamine and serotonin with immune state cd4 and cd8 of the Newcastle infection in broilers. Iraqi Journal of Agricultural Sciences. 54(4): 957-962.

Ulaiwi, A. H. 2023. DIVA molecular detection of suspected case of newcastle and encephalomylitis disease in layers. Iraqi Journal of Agricultural Sciences. 54(3): 890-895.

Vincent, J. B. 2000. The biochemistry of chromium. J. Nutr. 130(4): 715-718.

Xiao, F., D. Ao, B. Zhou, J. W. Spears, X. Lin and Y. Huang. 2016. Effects of supplemental chromium propionate on serum lipids, carcass traits, and meat quality of heat-stressed broilers. Biol. Trace Elem. Res. 176(2): 401-406.




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