THE INFLUENCE OF GENISTEIN IMPLANTATION ON OFFSPRING SEX RATIOS AND THEIR RELATION TO ESTROGEN LEVELS IN THE BLOOD OF IRAQI CHICKENS
DOI:
https://doi.org/10.36103/ijas.v54i4.1790Keywords:
phytoestrogen, primary and secondary sex ratios, Iraqi local chickens, Steroid hormones.Abstract
This study was carried out at the Poultry Research Station / Agricultural Research Department / Ministry of Agriculture, to investigate the effects of implanting genistein (GE) for the period of 1/February/2021 to 16/August/2021. into Iraqi local chickens at various ages on primary (PSF) and secondary (SSF) sex ratios of female, fertility (FE), and hatchability (HA) traits. At the age of 12 weeks, 100 hens and 20 roosters of Iraqi local chickens from the Poultry Research Station were used in this study. After numbering the hens, the birds were housed in individual cages and divided into four treatments (each with 25 chickens) as follows: T1: none implantation; T2, T3, and T4: implantation of 10 mg GE /kg weight at 14, 18, and 22 weeks of age, respectively. The experiment was divided into three periods, each for 28 weeks, and then rated according to the overall average and all of the traits studied. The results showed that implanting GE into hens had a positive influence on FE, PSF, SSF, and estrogen level (ES), especially at 18 weeks of age. There were also significant correlations between traits and ES in hens’ blood. It was also shown that the regression of most traits on ES is first order linear. As a result, it can be concluded that GE has a positive effect on ES, PSF, SSF, with the possibility of predicting sex ratios and sex offspring based on estrogen levels in the blood, and that implantation at 18 weeks of age has produced great results.
References
Al – Daraji, H. J. 2002. Effects of different cryoprotectants on the fertilizing capacity of frozen chicken semen. Iraqi J. of Agri. Sci. 33 (1): 207 – 212. Article
Al – Daraji, H. J. 2006. Influence of supplementing diluent of semen with pomegranate juice of roosters semen quality during in vitro storage. Iraqi J. of Agri. Sci. 37 (4): 149 – 158. Article.
Al – Daraji, H. J., R. H. Razuki, I. A. Abdul-Hassan and A. S. Ahmed. 2007. The relationship between the diluent provision of green tea infusion and lipid peroxidation during in vitro storage of roosters' semen. Iraqi Journal of Agricultural Science. 38(2):141 – 147. Article.
Al – Daraji, H. J., I. A. Abdul – Hassan, A. S. Ahmed, H. A. A. Mashadani, S. A. Naji and N. I. A. Hadithi. 2006. Effect of Uropygialectomy at two different ages on semen quality of white leghorn males. Iraqi J. of Agri. Sci. 37(1): 213 – 218. Article
Ataei, A. H. and F. Kırkpınar. 2021. Application of In-Ovo Injection of Some Substances for Manipulation of Sex and Improving Performance in Chicken. 5th International Students Science Congress. https://doi.org/10.52460/issc.2021.006. pp:1-8
Bautista, L.M., G. Silván, S. Cáceres, L. Martínez-Fernández, C. Bravo and J. C. Illera. 2013. Faecal sexual steroids in sex typing and endocrine status of great bustards. European Journal of Wildlife Research. 59 (6): 815 – 822. DOI 10.1007/s10344-013-0735-6
Bermann, M., A. Legarra, M. K. Hollifield, Y. Masuda, D. Lourenco and I. Misztal,. 2021. Validation of single step GBLUP genomic predictions from threshold models using the linear regression method: An application in chicken mortality. Journal of Animal Breeding and Genetics. 138 (1): 4 – 13. DOI: 10.1111/jbg.12507
Burhan, M., S. Oktavia and F. Fauziah,. 2020. Antioxidant activities of genistein: A review. Journal of Pharmaceutical Sciences and Medicine. 5 (10): 19 – 23. DOI: 10.47760/ijpsm.2020.v05i10.001
Chue, J. and C. A. Smith. 2011. Sex determination and sexual differentiation in the avian model. The FEBS journal. 278 (7): 1027 – 1034.
doi:10.1111/j.1742-4658.2011.08032.x
Domínguez-López, I., M. Yago-Aragón, A. Salas-Huetos, A. Tresserra-Rimbau and S. Hurtado-Barroso. 2020. Effects of dietary phytoestrogens on hormones throughout a human lifespan: A review. Nutrients. 12 (8): 2456. https://doi.org/10.3390/nu12082456
Duncan, D. B. 1955. Multiple range and multiple F tests. Biometrics. 11(1): 1 – 42. https://doi.org/10.2307/3001478
DuRant, S. E. , W.A. Hopkins, A. W. Carter, L.T., K. J. Navara and D. M. Hawley. 2016. Incubation temperature causes skewed sex ratios in a precocial bird. Journal of Experimental Biology. 219 (3): 4-2193)): 1961 – 1964. https://doi.org/10.1242/jeb.138263
Groothuis, T. G. G., B. Y. Hsu, N. Kumar and B. Tschirren,. 2019. Revisiting mechanisms and functions of prenatal hormone-mediated maternal effects using avian species as a model. Philosophical Transactions of the Royal Society B: Biological Sciences. 374 (B): 1 – 9. https://doi.org/10.1098/rstb.2018.0115
Guioli, S., D. Zhao, S. Nandi, M. Clinton and R. Lovell-Badge. 2020. Oestrogen in the chick embryo can induce chromosomally male ZZ left gonad epithelial cells to form an ovarian cortex that can support oogenesis. Development. 147 (4): dev181693. https://doi.org/10.1242/dev.181693
Hameed, A. S. S., P. S. Rawat, X. Meng and W. Liu. 2020. Biotransformation of dietary phytoestrogens by gut microbes: A review on bidirectional interaction between phytoestrogen metabolism and gut microbiota. Biotechnology Advances. 43: 107576. DOI: 10.1016/j.biotechadv.2020.107576
Hu, Y-Q, D-P. Bai, Y. Chen, Z-X. Lu, H-B. Zheng and F-Q. Xu. 2019. The degree of sex reversal in Muscovy ducks (Cairina moschata domestica) induced by an aromatase inhibitor. Sexual Development. 13 (3): 137 – 142. https://doi.org/10.1159/000502195
Ibarra, A. K. V., S. A. C. Pérez, A. A. Rodríguez, A. M. R. Torres, F. R. Hernández and R. C. Flores. 2020. In vitro sperm storage with poultry oviductal secretions. Veterinary Research Forum. 11 (3): 207 – 211. doi: 10.30466/vrf.2019.95854.2300
Abdel Hassan, I. A. R., H. J. Al-Daraji and D. H. Al-Hasani. 2006. Some physiological traits of hisex brown layers fed with supplemental sodium bicarbonate during heat stress. Iraqi J. of Agri. Sci. 37(4): 159 – 164. Article
Kalina, J., J. Mucksová, H. Yan and P. Trefil. 2012. Rapid sexing of selected Galliformes by polymerase chain reaction. Czech Journal Animal Science. 57(4): 187 – 192
Kasim, W. Y., S. A. Alshaheen and M. H. AL-Asadi. 2014. The effect of genistein on some productive and biochemical blood traits of quail (Coturnix coturnix japonica). Basrah Journal of Veterinary Research. 1 (1): 60 – 69. Article
Kupiec, T. C., P. Matthews and R. Ahmad. 2000. Dry-heat sterilization of parenteral oil vehicles. International journal of pharmaceutical compounding. 4(3): 223 – 224. Article
Love, O. P. and T. D. Williams. 2008. The adaptive value of stress-induced phenotypes: effects of maternally derived corticosterone on sex-biased investment, cost of reproduction, and maternal fitness. The American Naturalist. 172(4): 135 – 149. Article
Lv, Z., Xing, K., Li, G., Liu, D., and Guo, Y. 2018. Dietary genistein alleviates lipid metabolism disorder and inflammatory response in laying hens with fatty liver syndrome. Frontiers in Physiology. 9, 1493. https://doi.org/10.3389/fphys.2018.01493
Major, A.T. and C. A. Smith. 2016. Sex reversal in birds. Sexual Development.10 (5-6): 288 – 300. https://doi.org/10.1159/000448365
Nanda, I., K. Schlegelmilch, T. Haaf, M. Schartl and M. Schmid. 2008. Synteny conservation of the Z chromosome in 14 avian species (11 families) supports a role for Z dosage in avian sex determination. Cytogenetic and Genome Research.122 (2): 150 – 155. DOI: 10.1159/000163092
National Research Council. 1994. Nutrient Requirements of Poultry. 9th editor. Washington, D. C: National Academy Press. pp : 61
Navara, K. J. 2013. The role of steroid hormones in the adjustment of primary sex ratio in birds: compiling the pieces of the puzzle. Integrative and Comparative Biology. 53 (6): 923 – 937. https://doi.org/10.1093/icb/ict083
Ohbuchi, K., T. 2021. Hirokawa. Protein druggability assessment for natural products using in silico simulation: A case study with estrogen receptor and the flavonoid genistein. Gene. 791 145726. https://doi.org/10.1016/j.gene.2021.145726
Paitz, R.T., R. Angles and E. Cagney. 2020. In ovo metabolism of estradiol to estrone sulfate in chicken eggs: implications for how yolk estradiol influences embryonic development. General and Comparative Endocrinology. 287:113320.https://doi.org/10.1016/j.ygcen.2019.113320
Patel S., A. Homaei, A. B. Raju and B. R. Meher. 2018. The necessary evil for human health, and ways to tame it. Biomedicine and Pharmacotherapy.102:403 – 411. https://doi.org/10.1016/j.biopha.2018.03.078
Pike A. C., A. M. Brzozowski, R. E. Hubbard, T. Bonn, A. G. Thorsell and O. Engström. 1999. Structure of the ligand binding domain of oestrogen receptor beta in the presence of a partial agonist and a full antagonist. The EMBO Journal. 18 (17): 4608 – 4618. https://doi.org/10.1093/emboj/18.17.4608
Saberifar T, F. Samadi, B. Dastar, S. Hasani, M. Kazemifard and F. Ganji. 2021. Enhancement of productive performance, bone physical gharacteristics, and mineralization of Laying hens during the post-peak period by genistein. Archives of Razi Institute. 76 (2): 399 – 409. doi: 10.22092/ari.2020.342143.1454
Schwabl H. 1996. Environment modifies the testosterone levels of a female bird and its eggs. Journal of Experimental Zoology. 276 (2): 157 – 163. https://doi.org/10.1002/(SICI)1097-010X(19961001)276:2<157::AID-JEZ9>3.0.CO;2-N
Smith C. A., and A. H. Sinclair. 2004. Sex determination: insights from the chicken. Bioessays. 26 (2): 120 – 132. https://doi.org/10.1002/bies.10400
Takimoto C. H., K. Glover, X. Huang, S. A. Hayes, L. Gallot, and M. Quinn. 2003. Phase I pharmacokinetic andpharmacodyna-mic analysis of unconjugated soy isoflavones administered to individuals with cancer. Cancer Epidemiology and Prevention Biomarkers. 12 (11): 1213 – 1221. Article
Thangavel, P., A. Puga-Olguín, J. F. Rodríguez-Landa and R. C. Zepeda. 2019. Genistein as potential therapeutic candidate for menopausal symptoms and other related diseases. Molecules. 24 (21): 1 – 17. doi:10.3390/molecules24213892
Vizcarra, J., R. Alan, J. Kirby. 2015. Reproduction in Male Birds. Sturkie's Eavian Physiology, 6th: Elsevier. Academic Press. 667 – 693. https://doi.org/10.1016/B978-0-12-819770-7.00022-0
von Engelhardt, N., C. Dijkstra, S. Daan and T. G. Groothuis. 2004. Effects of 17-β-estradiol treatment of female zebra finches on offspring sex ratio and survival. Hormones and Behavior. 45 (5): 306 – 313. https://doi.org/10.1016/j.yhbeh.2003.12.009
Wagner, W. E. 2019. Using IBM® SPSS® statistics for research methods and social science statistics, 7th. Sage Publications. PP: 255
Wu G-J., J-T. Chen, Y-G. Cherng, C-C. Chang, S-H. Liu and R-M. Chen. 2020. Genistein improves bone healing via triggering estrogen receptor alpha-mediated expressions of osteogenesis-associated genes and consequent maturation of osteoblasts. Journal of Agricultural and Food Chemistry.68(39): 10639 – 10650. https://doi.org/10.1021/acs.jafc.0c02830
Wuttke W., H. Jarry and D. Seidlová-Wuttke. 2007. Isoflavones—safe food additives or dangerous drugs. Ageing research reviews. 6 (2): 150 – 188. https://doi.org/10.1016/j.arr.2007.05.001
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