DIVA MOLECULAR DETECTION OF SUSPECTED CASE OF NEWCASTLE AND ENCEPHALOMYLITIS DISEASE IN LAYERS
DOI:
https://doi.org/10.36103/ijas.v54i3.1778Keywords:
M gene, F gene, one-step RT-PCR, meso-velogenic ND.Abstract
The aim of the study investigates the molecular diagnosis between (the Avian encephalomyelitis virus and Newcastle disease virus and differentiating the infected from vaccinated animals (DIVA) strategies. The 1st detection by RT-PCR for(PMV-1) as primary detection then, the samples were prepared as in FTA card Whatman® and sent to (AniCon Labs) Germany to detect by one step RT-PCR (DIVA technique) between NDV and AEV genotype. The result of the molecular investigation as primary detection revealed three samples were positive with Ct values (34.0, 26.6, and 35.8) respectively. Then, in the detection of AEV the result showed all samples were negative. finally, by using (DIVA) with two primers to detect general (PMV1-9) by (M-gene) all samples were positive at Ct values (20.8, 20.1, and 25.2) respectively, and then path type by (F gene) to differentiate infected from vaccinated samples also all samples were positive with Ct values (23.8, 23.3 and 23.1) respectively. the study concluded that the samples can differentiate between AEV and NDV by using DIVA strategies to find pathotypes and differentiating between infected and vaccinal isolate based on certain amino acids in the primers.
References
Abolnik, C. 2017. History of Newcastle disease in South Africa. Onderstepoort J. Vet. Res. 84(4):1–7.
Ahmed, A.I. and S.M. Odisho.2018. Isolation identification and pathotyping of newcastle disease viruses from naturally infected chickens in Iraqi Kurdistan region. Iraqi J. Agri. Sci. 49(1):132-14. DOI: https://doi.org/10.36103/ijas.v49i1.216
Ali, Z., W. S. Taohid, M. M. Mohammad, B. Akramul, A. Al Momen, A.K. Shamsul, A.B. Zafar and M. Giasuddin. 2021. First report on the seroprevalence of avian encephalomyelitis virus antibody in Sonali (cross-bred) chickens in Bogura, Bangl.Vet. 8(1): 78–83.
Alphin, R.L., C.D. Ciaverelli, D.P. Hougentogler, K.J. Johnson, M.K. Rankin and E.R. Benson. 2010. Post outbreak disinfection of mobile equipment. Avian Dis. 54(1):772–776
Al-Shammari, A.A., A. Huda and A. M. Murtadha.2014. Molecular diagnosis of Newcastle disease Iraqi Virulent strain virus HN gene by specific primers design.2014. Kufa J. Vet. Med. Sci.5(2):196-203
Ayoub, M.A., W. K. Elfeil, D. El Boraey, H. Hammam and M. A. Nossair. 2019. Evaluation of some vaccination programs in protection of experimentally challenged broiler chicken against newcastle disease virus. Am. J. Anim. Vet. Sci. 14(3): 197-206
Barros, M.E., B. P. Rocha, F.A. Souza, F. S. Mendonça and J. Evencio-Neto. 2019. Severe outbreak of avian encephalomyelitis in laying hens in northeastern brazil. Braz J Poult. Sci. 21 (02):1–4.
Beenish, Z., I. Q. Javed, Z. Amir, A. Asim, A. Raheela, S. Haleema, A. Qurat. S. Razia, I. Irfan and A. Sobia. 2020. Detection and molecular characterization of virulent Newcastle disease virus in ducks (Anas platyrhynchos domesticus). Pakistan J. Zool.52(1):1-4.
Chen, H.T., J. Zhang, D. H. Sun, J. L. Zhang, X. P. Cai, X. T. Liu, Y. Z. Ding, L. N. Ma, S. H. Yang, L. Jin and Y. Liu. 2019. Development and application of multiplex PCR method for simultaneous detection of seven viruses in ducksVet. Res. Commun., 15(103): 1-10.
Chen, J.P. and C.H. Wang. 2002. Clinical epidemiologic and experimental evidence for the transmission of Newcastle disease virus through eggs. Avian Dis. 46(2):461–465
Cucco, M., I. Pellegrino and G. Malacarne. 2010. Immune challenge affects female condition and egg size in the grey partridge. J. Exp. Zool. A. Ecol. Genet. Physiol. 313(9):597–604.
Dimitrov, K.M., C. Abolnik, C. L. Afonso, E. Albina, J. Bahl, M. Berg, F.X. Briand, I. H. Brown, K. S. Choi and I. Chvala. 2019. Updated unified phylogenetic classification system and revised nomenclature for Newcastle disease virus. Infect. Genet. Evol. 74 (103917): 1-15.
Farkas,T., E. Sze´kely, S. Bela´k and I. Kiss.2009. Real-Time PCR-based pathotyping of newcastle disease virus by use of TaqMan minor groove binder probes.2009. J. clini. microbiol. 47(7): 2114–2123
Fatima, K.E, Y. A. Almofti, K. A. Abd-elrahman, M. A. Nouri and E. A. Eltilib. 2021. Structural analysis of avian encephalomyelitis virus polyprotein for development of multi epitopes vaccine using immunoinformatics approach. J. Pure. Appl. Microbiol.15(1):262-278
Freitas, E. S. and A. Back. 2015. New occurance of avian encephalomyelitis in broiler - is this an emerging disease. Braz. J. Poult. Sci.17(3):399–404
Hameed, S.S., Amjed H.U and Hamad S.M.2022. Diagnosis of E. coli isolated from arthritis in chicken by vitk and molecular methods. Iraqi J. Agri. Sci.53 (1),141-146. DOI: https://doi.org/10.36103/ijas.v53i1.1518
Hauck, R., C. G. Senties-Cue and Y. Wan. 2017. Evolution of avian encephalomyelitis virus during embryoadaptation. Vet. Microbiol. 204(12):1-7.
Hesham, A., K. Wael, A. Ahmed, T. Laila, G. Elsayed, M. Emad, A. Ahmad and T. Shaimaa. 2022. Efficacy of the newcastle disease virus genotype VII.1.1-matched vaccines in commercial broilers. Vaccines.10 (1): 1-17
Hussein, S. I., A.F. Khalaf, Y. Ahmed, B. Ahmed and A. Iyad. 2020. Determination of inhibition activity of α-amylase enzyme, antioxidant activity, antibacterial activity and phenolic compounds by using some medical plants. Iraqi J. Agri. Sci. 51(1):411-421.
DOI: https://doi.org/10.36103/ijas.v51i1.940
Knowles, N. J., T. Hovi, T. Hyypia, A. M. King and L. M. Lindberg. 2012. Family picornaviridae. In: King, A. M. Q., M. J. Adams, E. B. Carstens and E. J. Lefkowitz. editors. Virus taxonomy: classification and nomenclature of viruses: nineth report of the international committee on taxonomy of viruses. London: Elsevier. J. Gen. Virol.94(9): 2029–2035
Kommers, G.D., D.J. King, B.S. Seal and C.C. Brown. 2001. Virulence of pigeon‐origin Newcastle disease virus isolates for domestic chickens. Avian Dis. 45(4):906–921
Lau, S. K. P., P. C. Woo, C.C. Yip, K. S. Li, R. Y. Fan, R. Bai, Y. Huang, K. H. Chan and K. Y. Yuen. 2014. Chickens host diverse picornaviruses originated from potential interspecies transmission with recombination. J. Gen. Virol. 95(9):1929-1944
Magdalena, M., H. M. Bernd, G. Christian, R. O. Angela and C. M.Thomas. 2020. A novel recombinant newcastle disease virus vectored DIVA vaccine against peste des petits ruminants in goats. Vaccines.8(1): 205-211.
Mohammad, A.H., and A.S. Al-Hassani.2022. Effect of different levels of turmeric root powder to diet to some traits of broiler exposed to heat stress. Iraqi J. Agri. Sci. 53:(4), 950-957. DOI: https://doi.org/10.36103/ijas.v53i4.1607
Qiu, X., Y. Yu, S. Yu, Y. Zhan, N. Wei, C. Song, Y. Sun, L. Tan and C. Ding. 2014. Development of strand-specific Real-Time RT-PCR to distinguish viral RNAs during Newcastle disease virus infection. Sci. World J.Article ID 934851 01
Rahman, M.M., L. R. Barman, E. H. Chowdhury and M. R. Islam.2016. Detection of Newcastle disease virus of poultry by real time reverse transcription polymerase chain reaction. Bangl. Vet.33(1):16 – 22
Romer-Oberdorfer, A., O. Werner, J. Veits, T. Mebatsion and T. C. Mettenleiter. 2003. Contribution of the length of the HN protein and the sequence of the F protein cleavage site to Newcastle disease virus pathogenicity. J. Gen. Virol. 84 (11):3121-3129
Sameera, A., A.M. Muhammad, M. Khushi, Y. T. Muhammad, R. Masood, R. A. ul-Rahman and Z. S. Muhammad.2016. Genetic characterization and phylogeny of pigeon paramyxovirus isolate (PPMV-1) from Pakistan. Springer Plus. 5(1): 1295- 1312.
Shihab, I.M.2017. Effect of different levels of turmeric supplementation with diet on humoral immune response to newcastle and infectious bursal disease virus and histopathological changes to some internal organs of broiler chickens. Iraqi J. Agri. Sci. 48: (Special Issue): 041-044. DOI: https://doi.org/10.36103/ijas.v48iSpecial.251
Suarez, D. L. 2020. Newcastle Disease, Other Avian Paramyxoviruses, and Avian Metapneumovirus Infections. pp. 111–166. In: Diseases of Poultry, 14th ed. (Swayne, D. E., J. R. Glisson, L. R. McDougald, L.K. Nolan, D. L. Suarez and V. Nair. Wiley-Blackwell, Ames
Sultan, H.A. S. Talaat, W. K. Elfeil, K. Selim, M. A. Kutkat, S.A., Amer and K. S. Choi. 2020. Protective efficacy of the Newcastle disease virus genotype VII matched vaccine in commercial layers. Poult. Sci. 99(3):1275-1286
Xie, Z., M. I. Khan, T. Girshick and Z. Xie. 2005. Reverse transcriptase-polymerase chain reaction to detect avian encephalomyelitis virus. Avian Dis. 49(2):227-30.
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